diff --git a/test/io/configs/expected/aliases.config b/test/io/configs/expected/aliases.config
index fa4b22109dd..42e79283142 100644
--- a/test/io/configs/expected/aliases.config
+++ b/test/io/configs/expected/aliases.config
@@ -5,7 +5,6 @@ db-extra-search-path = "public"
 db-max-rows = 1000
 db-plan-enabled = false
 db-pool = 10
-db-pool-timeout = 3600
 db-pre-request = "check_alias"
 db-prepared-statements = true
 db-root-spec = "open_alias"
diff --git a/test/io/configs/expected/boolean-numeric.config b/test/io/configs/expected/boolean-numeric.config
index bfa15b0f093..245b9b54631 100644
--- a/test/io/configs/expected/boolean-numeric.config
+++ b/test/io/configs/expected/boolean-numeric.config
@@ -5,7 +5,6 @@ db-extra-search-path = "public"
 db-max-rows = ""
 db-plan-enabled = false
 db-pool = 10
-db-pool-timeout = 3600
 db-pre-request = ""
 db-prepared-statements = false
 db-root-spec = ""
diff --git a/test/io/configs/expected/boolean-string.config b/test/io/configs/expected/boolean-string.config
index bfa15b0f093..245b9b54631 100644
--- a/test/io/configs/expected/boolean-string.config
+++ b/test/io/configs/expected/boolean-string.config
@@ -5,7 +5,6 @@ db-extra-search-path = "public"
 db-max-rows = ""
 db-plan-enabled = false
 db-pool = 10
-db-pool-timeout = 3600
 db-pre-request = ""
 db-prepared-statements = false
 db-root-spec = ""
diff --git a/test/io/configs/expected/defaults.config b/test/io/configs/expected/defaults.config
index 6bdc3298d15..4b7560f8dbd 100644
--- a/test/io/configs/expected/defaults.config
+++ b/test/io/configs/expected/defaults.config
@@ -5,7 +5,6 @@ db-extra-search-path = "public"
 db-max-rows = ""
 db-plan-enabled = false
 db-pool = 10
-db-pool-timeout = 3600
 db-pre-request = ""
 db-prepared-statements = true
 db-root-spec = ""
diff --git a/test/io/configs/expected/no-defaults-with-db-other-authenticator.config b/test/io/configs/expected/no-defaults-with-db-other-authenticator.config
index 9a7c9b53751..e2fc5229de2 100644
--- a/test/io/configs/expected/no-defaults-with-db-other-authenticator.config
+++ b/test/io/configs/expected/no-defaults-with-db-other-authenticator.config
@@ -5,7 +5,6 @@ db-extra-search-path = "public,extensions,other"
 db-max-rows = 100
 db-plan-enabled = true
 db-pool = 1
-db-pool-timeout = 100
 db-pre-request = "test.other_custom_headers"
 db-prepared-statements = false
 db-root-spec = "other_root"
diff --git a/test/io/configs/expected/no-defaults-with-db.config b/test/io/configs/expected/no-defaults-with-db.config
index 413513efee3..ecc54bc3c63 100644
--- a/test/io/configs/expected/no-defaults-with-db.config
+++ b/test/io/configs/expected/no-defaults-with-db.config
@@ -5,7 +5,6 @@ db-extra-search-path = "public,extensions,private"
 db-max-rows = 1000
 db-plan-enabled = true
 db-pool = 1
-db-pool-timeout = 100
 db-pre-request = "test.custom_headers"
 db-prepared-statements = false
 db-root-spec = "root"
diff --git a/test/io/configs/expected/no-defaults.config b/test/io/configs/expected/no-defaults.config
index a995811d698..921cd7b3d1a 100644
--- a/test/io/configs/expected/no-defaults.config
+++ b/test/io/configs/expected/no-defaults.config
@@ -5,7 +5,6 @@ db-extra-search-path = "public,test"
 db-max-rows = 1000
 db-plan-enabled = true
 db-pool = 1
-db-pool-timeout = 100
 db-pre-request = "please_run_fast"
 db-prepared-statements = false
 db-root-spec = "openapi_v3"
diff --git a/test/io/configs/expected/types.config b/test/io/configs/expected/types.config
index b6dfe133309..b4b5f279513 100644
--- a/test/io/configs/expected/types.config
+++ b/test/io/configs/expected/types.config
@@ -5,7 +5,6 @@ db-extra-search-path = "public"
 db-max-rows = ""
 db-plan-enabled = false
 db-pool = 10
-db-pool-timeout = 3600
 db-pre-request = ""
 db-prepared-statements = true
 db-root-spec = ""
diff --git a/test/io/configs/no-defaults-env.yaml b/test/io/configs/no-defaults-env.yaml
index 59684b63099..7ada3625699 100644
--- a/test/io/configs/no-defaults-env.yaml
+++ b/test/io/configs/no-defaults-env.yaml
@@ -7,7 +7,6 @@ PGRST_DB_EXTRA_SEARCH_PATH: public, test
 PGRST_DB_MAX_ROWS: 1000
 PGRST_DB_PLAN_ENABLED: true
 PGRST_DB_POOL: 1
-PGRST_DB_POOL_TIMEOUT: 100
 PGRST_DB_PREPARED_STATEMENTS: false
 PGRST_DB_PRE_REQUEST: please_run_fast
 PGRST_DB_ROOT_SPEC: openapi_v3
diff --git a/test/io/configs/no-defaults.config b/test/io/configs/no-defaults.config
index e2468295431..6c67ad15605 100644
--- a/test/io/configs/no-defaults.config
+++ b/test/io/configs/no-defaults.config
@@ -5,7 +5,6 @@ db-extra-search-path = "public, test"
 db-max-rows = 1000
 db-plan-enabled = true
 db-pool = 1
-db-pool-timeout = 100
 db-pre-request = "please_run_fast"
 db-prepared-statements = false
 db-root-spec = "openapi_v3"
diff --git a/test/io/test_io.py b/test/io/test_io.py
index 063911dfb9f..081bad107ff 100644
--- a/test/io/test_io.py
+++ b/test/io/test_io.py
@@ -167,7 +167,6 @@ def run(
     "Run PostgREST and yield an endpoint that is ready for connections."
     env = env or {}
     env["PGRST_DB_POOL"] = "1"
-    env["PGRST_DB_POOL_TIMEOUT"] = "1"
 
     with tempfile.TemporaryDirectory() as tmpdir:
         if port: