From 3c3b9b1a3c849d59cc93467eb3da6b4527bcf469 Mon Sep 17 00:00:00 2001 From: David Rach <91153676+DavidRach@users.noreply.github.com> Date: Wed, 23 Oct 2024 02:08:32 -0400 Subject: [PATCH] Initial Readme landing page. --- README.md | 8 ++++++++ RedditExample_CompensationR.qmd | 3 ++- 2 files changed, 10 insertions(+), 1 deletion(-) create mode 100644 README.md diff --git a/README.md b/README.md new file mode 100644 index 0000000..0413c01 --- /dev/null +++ b/README.md @@ -0,0 +1,8 @@ +## Cytometry in R: Examples + +So you want to analyze Cytometry data in R, and are struggling with your code? We were in your shoes not that long-ago!. + +This repository represents our contribution back to the broader cytometry community in the form of code and code-explanations. + +We hope this reduces the barriers (and similarly gnashing of teeth, hair-pulling, and cursing outburst directed at your code) that we experienced when first getting started. + diff --git a/RedditExample_CompensationR.qmd b/RedditExample_CompensationR.qmd index 79ebbea..7e1a57a 100644 --- a/RedditExample_CompensationR.qmd +++ b/RedditExample_CompensationR.qmd @@ -129,9 +129,10 @@ flowframe_comped <- compensate(flowframe, TheComps[[1]]) head(flowframe_comped@exprs, 10) ``` -# Visualizing (ggcyto) As you might be able to tell, FSC SSC parameters remain the same, while the values for the Fluorophore columns have been adjusted. This is due to no columns being present for FSC SSC Time etc. in the Spillover matrix. +# Visualizing (ggcyto) + We can visualize this with the with the `r Biocpkg("ggcyto")` package to see the effects for the before vs. after: ```{r}