Run docker steps, now what?? #407
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faizjab
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* General SCENIC questions
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I am running pySCENIC in command line via docker. I have run the three Docker steps:
docker run -it --rm
-v /data:/data
aertslab/pyscenic:0.12.0 pyscenic grn
--num_workers 6
-o /data/expr_mat.adjacencies.tsv
/data/expr_mat.tsv
/data/allTFs_hg38.txt
docker run -it --rm
-v /data:/data
aertslab/pyscenic:0.12.0 pyscenic ctx
/data/expr_mat.adjacencies.tsv
/data/hg19-tss-centered-5kb-7species.mc9nr.genes_vs_motifs.rankings.feather
/data/hg19-tss-centered-10kb-7species.mc9nr.genes_vs_motifs.rankings.feather
--annotations_fname /data/motifs-v9-nr.hgnc-m0.001-o0.0.tbl
--expression_mtx_fname /data/expr_mat.tsv
--mode "custom_multiprocessing"
--output /data/regulons.csv
--num_workers 6
docker run -it --rm
-v /data:/data
aertslab/pyscenic:0.12.0 pyscenic aucell
/data/expr_mat.tsv
/data/regulons.csv
-o /data/auc_mtx.csv
--num_workers 6
I have now got three files:
expr_mat.tsv
regulons.csv
auuc_mtx.csv
What do I do now?
How do I complete step 8 (clustering and projection)?
After that how do I create an integrated loom file for visualisation in SCope?
Any help would be greatly appreciated
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