diff --git a/modules/illumina.nf b/modules/illumina.nf index ba702a3..056a7cc 100644 --- a/modules/illumina.nf +++ b/modules/illumina.nf @@ -264,7 +264,7 @@ process callConsensusFreebayes { done # apply ambiguous variants first using IUPAC codes. this variant set cannot contain indels or the subsequent step will break - bcftools consensus -f ${ref} -I ${sampleName}.ambiguous.norm.vcf.gz > ${sampleName}.ambiguous.fa + bcftools consensus -s - -f ${ref} -I ${sampleName}.ambiguous.norm.vcf.gz > ${sampleName}.ambiguous.fa # Get viral contig name from reference CTG_NAME=\$(head -n1 ${ref} | sed 's/>//') diff --git a/nextflow.config b/nextflow.config index d1a9373..3cc794e 100644 --- a/nextflow.config +++ b/nextflow.config @@ -5,7 +5,7 @@ manifest { description = 'Epi2me compatible Nextflow pipeline for processing ARTIC tiling amplicon Illumina sequencing reads from monkeypox virus (MPXV) samples.' mainScript = 'main.nf' nextflowVersion = '>=20.01.0' - version = '1.0.12' + version = '1.0.13' } // Load base.config by default for all pipelines