From f00476fe87ee28a40f7a89e886630f92a2f08924 Mon Sep 17 00:00:00 2001 From: josschavezf Date: Mon, 20 May 2024 09:55:42 -0400 Subject: [PATCH] update images width --- vignettes/nemo_slideseq.Rmd | 35 +++++++++++++++++++++++++++-------- 1 file changed, 27 insertions(+), 8 deletions(-) diff --git a/vignettes/nemo_slideseq.Rmd b/vignettes/nemo_slideseq.Rmd index 01c412e1c..fe300c4a5 100644 --- a/vignettes/nemo_slideseq.Rmd +++ b/vignettes/nemo_slideseq.Rmd @@ -11,32 +11,49 @@ vignette: > Log in to your [Terra bio](https://app.terra.bio/) account and go to the left menu, select Library > Datasets -![](images/nemo_slideseq/01_menu.png) +```{r, echo=FALSE, out.width='30%', fig.align='center'} +knitr::include_graphics("images/nemo_slideseq/01_menu.png") +``` On the datasets page, scroll down until you find the NeMO database -![](images/nemo_slideseq/02_datasets.png) +```{r, echo=FALSE, out.width='80%', fig.align='center'} +knitr::include_graphics("images/nemo_slideseq/02_datasets.png") +``` -![](images/nemo_slideseq/03_datasets.png) +```{r, echo=FALSE, out.width='80%', fig.align='center'} +knitr::include_graphics("images/nemo_slideseq/03_datasets.png") +``` # Load the dataset to a terra workspace By clicking on the Browse data button, you will be re-directed to the NeMO website. Use the filters menu to select the Slide-seq technology, then chose a sample to download and ddd the file to the cart. -![](images/nemo_slideseq/04_nemo.png) -![](images/nemo_slideseq/05_samples.png) + +```{r, echo=FALSE, out.width='80%', fig.align='center'} +knitr::include_graphics("images/nemo_slideseq/04_nemo.png") +``` + + +```{r, echo=FALSE, out.width='80%', fig.align='center'} +knitr::include_graphics("images/nemo_slideseq/05_samples.png") +``` When selecting the sample, you will see multiple files available to download, including the fasta files. Uncheck the fasta files boxes and keep only the expression.mex.tar.gz file. Click the Download button and select the option "Export to terra". -![](images/nemo_slideseq/06_files.png) +```{r, echo=FALSE, out.width='80%', fig.align='center'} +knitr::include_graphics("images/nemo_slideseq/06_files.png") +``` You will be directed back to your terra bio account. Select the Workspace to add your dataset. You will find the new file under the Data tab > file section. -![](images/nemo_slideseq/07_data.png) +```{r, echo=FALSE, out.width='90%', fig.align='center'} +knitr::include_graphics("images/nemo_slideseq/07_data.png") +``` # Open the dataset @@ -46,7 +63,9 @@ https://data.nemoarchive.org/biccn/grant/rf1_macosko/macosko/spatial_transcripto Open an cloud environment, either using Jupyter notebooks or RStudio. Open the terminal and load the file to your session by running the command `wget `. Uncompress the file, you will get a folder with three files: -![](images/nemo_slideseq/08_files.png) +```{r, echo=FALSE, out.width='50%', fig.align='center'} +knitr::include_graphics("images/nemo_slideseq/08_files.png") +``` Use these files to create a Giotto object and start running the analysis.