Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

Header not contain PG line and duplicate SQ line #20

Open
manburst opened this issue Apr 6, 2021 · 0 comments
Open

Header not contain PG line and duplicate SQ line #20

manburst opened this issue Apr 6, 2021 · 0 comments

Comments

@manburst
Copy link

manburst commented Apr 6, 2021

Hello
I have tried to align with command ./graphmap2/bin/Linux-x64/graphmap2 align -r ref/ecoliref.fa --gtf ref/ecoliref.gtf -d tex1/tex/call_pass/newbasecalltest/high_tex_chop_trim.fastq -o tex1/tex/call_pass/newbasecalltest/quality_graph.sam
Then I need to convert it into a bam file but it showed an error that the PG line is duplicate and also PG line is missing so I cannot convert them.
Both references were downloaded from ensembl.
Any suggestion to solve this?
Here is a screenshot from sam file output
image
Thanks in advance.

Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Labels
None yet
Projects
None yet
Development

No branches or pull requests

1 participant