diff --git a/client/src/api/schema/schema.ts b/client/src/api/schema/schema.ts
index 1ec1d6fc39d1..1f4dabcf05ed 100644
--- a/client/src/api/schema/schema.ts
+++ b/client/src/api/schema/schema.ts
@@ -9254,7 +9254,7 @@ export interface components {
              * Type
              * @enum {string}
              */
-            type: "ftp" | "posix" | "s3fs" | "azure" | "onedata" | "webdav" | "dropbox" | "googledrive";
+            type: "ftp" | "posix" | "s3fs" | "azure" | "onedata" | "webdav" | "dropbox" | "googledrive" | "elabftw";
             /** Variables */
             variables?:
                 | (
@@ -17599,7 +17599,7 @@ export interface components {
              * Type
              * @enum {string}
              */
-            type: "ftp" | "posix" | "s3fs" | "azure" | "onedata" | "webdav" | "dropbox" | "googledrive";
+            type: "ftp" | "posix" | "s3fs" | "azure" | "onedata" | "webdav" | "dropbox" | "googledrive" | "elabftw";
             /** Uri Root */
             uri_root: string;
             /**
diff --git a/client/src/components/FileSources/FileSourceTypeSpan.vue b/client/src/components/FileSources/FileSourceTypeSpan.vue
index 890649e4cf86..8d6f5d6324b7 100644
--- a/client/src/components/FileSources/FileSourceTypeSpan.vue
+++ b/client/src/components/FileSources/FileSourceTypeSpan.vue
@@ -12,6 +12,7 @@ const MESSAGES = {
     webdav: "This is a remote file source plugin based on the WebDAV protocol.",
     dropbox: "This is a file source plugin that connects with the commercial Dropbox service.",
     googledrive: "This is a file source plugin that connects with the commercial Google Drive service.",
+    elabftw: "This is a remote file source that connects with an eLabFTW instance.",
 };
 
 interface Props {
diff --git a/doc/source/admin/data.md b/doc/source/admin/data.md
index 15fbecbade4a..f0c4e827d08b 100644
--- a/doc/source/admin/data.md
+++ b/doc/source/admin/data.md
@@ -373,6 +373,19 @@ configuration).
 
 ![](file_source_dropbox_configuration.png)
 
+#### `elabftw`
+
+The syntax for the `configuration` section of `elabftw` templates looks like this.
+
+![](file_source_elabftw_configuration_template.png)
+
+At runtime, after the `configuration` template is expanded, the resulting dictionary
+passed to Galaxy's file source plugin infrastructure looks like this and should match a subset
+of what you'd be able to add directly to `file_sources_conf.yml` (Galaxy's global file source
+configuration).
+
+![](file_source_elabftw_configuration.png)
+
 ### YAML Syntax
 
 ![galaxy.files.templates.models](file_source_templates.png)
diff --git a/doc/source/admin/file_source_elabftw_configuration.png b/doc/source/admin/file_source_elabftw_configuration.png
new file mode 100644
index 000000000000..f7dacc2f520c
Binary files /dev/null and b/doc/source/admin/file_source_elabftw_configuration.png differ
diff --git a/doc/source/admin/file_source_elabftw_configuration_template.png b/doc/source/admin/file_source_elabftw_configuration_template.png
new file mode 100644
index 000000000000..a830dfaa1e36
Binary files /dev/null and b/doc/source/admin/file_source_elabftw_configuration_template.png differ
diff --git a/doc/source/admin/gen_diagrams.py b/doc/source/admin/gen_diagrams.py
index 3e1d57a53be4..0f54f6e7bdcb 100644
--- a/doc/source/admin/gen_diagrams.py
+++ b/doc/source/admin/gen_diagrams.py
@@ -10,6 +10,8 @@
     AzureFileSourceTemplateConfiguration,
     DropboxFileSourceConfiguration,
     DropboxFileSourceTemplateConfiguration,
+    eLabFTWFileSourceConfiguration,
+    eLabFTWFileSourceTemplateConfiguration,
     FileSourceTemplate,
     FtpFileSourceConfiguration,
     FtpFileSourceTemplateConfiguration,
@@ -61,6 +63,8 @@
     FtpFileSourceConfiguration: "file_source_ftp_configuration",
     WebdavFileSourceTemplateConfiguration: "file_source_webdav_configuration_template",
     WebdavFileSourceConfiguration: "file_source_webdav_configuration",
+    eLabFTWFileSourceTemplateConfiguration: "file_source_elabftw_configuration_template",
+    eLabFTWFileSourceConfiguration: "file_source_elabftw_configuration",
 }
 
 for clazz, diagram_name in class_to_diagram.items():
diff --git a/doc/source/admin/search_for_new_screenshots.py b/doc/source/admin/search_for_new_screenshots.py
index ea09034da8d4..7007d3a32ecf 100644
--- a/doc/source/admin/search_for_new_screenshots.py
+++ b/doc/source/admin/search_for_new_screenshots.py
@@ -23,6 +23,7 @@
     "user_file_source_form_full_azure.png",
     "user_file_source_form_full_ftp.png",
     "user_file_source_form_full_webdav.png",
+    "user_file_source_form_full_elabftw.png",
 ]
 
 
diff --git a/doc/source/admin/user_file_source_form_full_elabftw.png b/doc/source/admin/user_file_source_form_full_elabftw.png
new file mode 100644
index 000000000000..0ecfe98c78bb
Binary files /dev/null and b/doc/source/admin/user_file_source_form_full_elabftw.png differ
diff --git a/lib/galaxy/files/sources/elabftw.py b/lib/galaxy/files/sources/elabftw.py
index bcb0449fbbf5..a3e8439fc73a 100644
--- a/lib/galaxy/files/sources/elabftw.py
+++ b/lib/galaxy/files/sources/elabftw.py
@@ -6,15 +6,21 @@
 overview, try out the live demo [4]. The scope of this implementation is exporting data from and importing data to
 eLabFTW as file attachments of *already existing* experiments and resources. Each user can configure their preferred
 eLabFTW instance entering its URL and an API Key.
-
 File sources reference files via a URI, while eLabFTW uses auto-incrementing positive integers. For more details read
 galaxyproject/galaxy#18665 [5]. This leads to the need to declare a mapping between said identifiers and Galaxy URIs.
 
 Those take the form ``elabftw://demo.elabftw.net/entity_type/entity_id/attachment_id``, where:
+
 - ``entity_type`` is either 'experiments' or 'resources'
 - ``entity_id`` is the id (an integer in string form) of an experiment or resource
 - ``attachment_id`` is the id (an integer in string form) of an attachment
 
+For the user-defined file sources use case (when users configure one or more instances of the FilesSource via a file
+source template), Galaxy URIs have a different scheme and authority, taking the form ``gxuserfiles://file_source_id/
+entity_type/entity_id/attachment_id``, where:
+
+- ``file_source_id`` is the file source identifier assigned by Galaxy
+
 This implementation uses both ``aiohttp`` and the ``requests`` libraries as underlying mechanisms to communicate with
 eLabFTW via its REST API [6]. A significant limitation of the implementation is that, due to the fact that the API does
 not have an endpoint that can list attachments for several experiments and/or resources with a single request, when
@@ -27,6 +33,7 @@
 unknown.
 
 References:
+
 - [1] https://www.elabftw.net/
 - [2] https://doc.elabftw.net/user-guide.html#experiments
 - [3] https://doc.elabftw.net/user-guide.html#resources
@@ -47,6 +54,7 @@
 from textwrap import dedent
 from time import time
 from typing import (
+    Any,
     AsyncIterator,
     cast,
     Dict,
@@ -78,6 +86,7 @@
 from galaxy.files.sources import (
     AnyRemoteEntry,
     BaseFilesSource,
+    DEFAULT_SCHEME,
     FilesSourceOptions,
     FilesSourceProperties,
     PluginKind,
@@ -169,14 +178,29 @@ def __init__(self, *args, **kwargs: Unpack[eLabFTWFilesSourceProperties]):
         self._endpoint = kwargs["endpoint"]  # meant to be accessed only from `_get_endpoint()`
         self._api_key = kwargs["api_key"]  # meant to be accessed only from `_create_session()`
 
-    def get_prefix(self) -> Optional[str]:
-        return None
+    def get_prefix(self, user_context: OptionalUserContext = None) -> Optional[str]:
+        endpoint: ParseResult = self._get_endpoint(user_context=user_context)
+        return self.id if self.scheme not in {"elabftw", DEFAULT_SCHEME} else (endpoint.netloc or None)
+        # it would make better sense to return
+        # `self.id if self.scheme == USER_FILE_SOURCES_SCHEME else (endpoint.netloc or None)`, where
+        # `USER_FILE_SOURCES_SCHEME` comes from `galaxy.managers.file_source_instances`; however, that would lead to a
+        # circular import (maybe `USER_FILE_SOURCES_SCHEME` should be moved to a module in a layer deeper than
+        # `galaxy.managers`)
 
     def get_scheme(self) -> str:
-        return "elabftw"
+        return self.scheme if self.scheme and self.scheme != DEFAULT_SCHEME else "elabftw"
+        # it would make better sense to return `self.scheme if self.scheme == USER_FILE_SOURCES_SCHEME else "elabftw"`,
+        # but the same circular import issue as above arises
 
-    def get_uri_root(self) -> str:
-        return super().get_uri_root()
+    def score_url_match(self, url: str) -> int:
+        parsed_url = urlparse(url)
+        return sum(
+            int(check)
+            for check in (
+                parsed_url.scheme == self.get_scheme(),
+                parsed_url.netloc == self.get_prefix(),
+            )
+        )
 
     def to_relative_path(self, url: str) -> str:
         parsed_url = urlparse(url)
@@ -225,9 +249,11 @@ def _get_session_headers(
 
         Meant to be used only by `_create_session()` and `_create_session_async()`.
         """
-        props = dict(
-            **(options.extra_props if options and options.extra_props else {}),
-            **self._serialization_props(user_context),
+        props = {}
+        props.update(self._props)
+        props.update(options.extra_props if options and options.extra_props else {})
+        props.update(
+            {key: value for key, value in self._serialization_props(user_context).items() if value is not None}
         )
         headers = {
             "Authorization": props.get("api_key", self._api_key),
@@ -243,9 +269,11 @@ def _get_endpoint(
         """
         Retrieve the endpoint from the constructor, or override it via a :class:`FileSourceOptions` object.
         """
-        props = dict(
-            **(options.extra_props if options and options.extra_props else {}),
-            **self._serialization_props(user_context),
+        props = {}
+        props.update(self._props)
+        props.update(options.extra_props if options and options.extra_props else {})
+        props.update(
+            {key: value for key, value in self._serialization_props(user_context).items() if value is not None}
         )
         endpoint = props.get("endpoint", self._endpoint)
         # given that `options.extra_props` is of `eLabFTWFilesSourceProperties` type, it should be a string
@@ -254,9 +282,14 @@ def _get_endpoint(
         return urlparse(endpoint)
 
     def _serialization_props(self, user_context: OptionalUserContext = None) -> eLabFTWFilesSourceProperties:
-        effective_props = {}
+        effective_props: Dict[str, Any] = {}
 
         for key, val in self._props.items():
+            if key in {"api_key", "endpoint"} and user_context is None:
+                # prevent exception while expanding `${user.user_vault.read_secret('preferences/elabftw/api_key')}` or
+                # `${user.preferences['elabftw|endpoint']}` without `user_context`
+                effective_props[key] = None
+                continue
             effective_props[key] = self._evaluate_prop(val, user_context=user_context)
 
         return cast(eLabFTWFilesSourceProperties, effective_props)
@@ -383,6 +416,7 @@ async def collect_async_iterator(async_iter: AsyncIterator) -> list:
                             self._yield_entity_types(
                                 endpoint,
                                 session,
+                                user_context=user_context,
                             )
                         )
                     )
@@ -423,6 +457,7 @@ async def collect_async_iterator(async_iter: AsyncIterator) -> list:
                                     else None
                                 ),
                                 writable=self.writable,
+                                user_context=user_context,
                             )
                         )
                     )
@@ -453,6 +488,7 @@ async def collect_async_iterator(async_iter: AsyncIterator) -> list:
                                 cast(str, wrapped_entity.entity_id) if retrieve_entities else cast(str, entity_id),
                                 endpoint,
                                 session,
+                                user_context=user_context,
                             )
                         )
                     )
@@ -530,9 +566,11 @@ async def collect_async_iterator(async_iter: AsyncIterator) -> list:
         # always matches such value.
         return (entries := [wrapped_entry.entry for wrapped_entry in wrapped_entries]), len(entries)
 
-    @staticmethod
     async def _yield_entity_types(
-        endpoint: ParseResult, session: aiohttp.ClientSession
+        self,
+        endpoint: ParseResult,
+        session: aiohttp.ClientSession,
+        user_context: OptionalUserContext = None,
     ) -> AsyncIterator[eLabFTWRemoteEntryWrapper[RemoteDirectory]]:
         """
         List the root directory, i.e. "/".
@@ -563,7 +601,7 @@ async def _yield_entity_types(
             RemoteDirectory(
                 **{
                     "name": "Experiments",
-                    "uri": f"elabftw://{endpoint.netloc}/experiments",
+                    "uri": f"{self.get_scheme()}://{self.get_prefix(user_context=user_context)}/experiments",
                     "path": "/experiments",
                     "class": "Directory",
                 }
@@ -573,7 +611,7 @@ async def _yield_entity_types(
             RemoteDirectory(
                 **{
                     "name": "Resources",
-                    "uri": f"elabftw://{endpoint.netloc}/resources",
+                    "uri": f"{self.get_scheme()}://{self.get_prefix(user_context=user_context)}/resources",
                     "path": "/resources",
                     "class": "Directory",
                 }
@@ -583,8 +621,8 @@ async def _yield_entity_types(
         yield experiments
         yield resources
 
-    @staticmethod
     async def _yield_entities(
+        self,
         entity_type: str,
         endpoint: ParseResult,
         session: aiohttp.ClientSession,
@@ -593,6 +631,7 @@ async def _yield_entities(
         query: Optional[str] = None,
         order: Optional[str] = None,
         writable: bool = False,
+        user_context: OptionalUserContext = None,
     ) -> AsyncIterator[eLabFTWRemoteEntryWrapper[RemoteDirectory]]:
         """List an entity type, i.e. either "/experiments" or "/resources"."""
         url = urljoin(
@@ -659,7 +698,10 @@ def validate_and_register_entity(item, mapping: Dict[int, dict]) -> Literal[True
                     RemoteDirectory(
                         **{
                             "name": entity["title"],
-                            "uri": f"elabftw://{endpoint.netloc}/{entity_type}/{entity['id']}",
+                            "uri": (
+                                f"{self.get_scheme()}://{self.get_prefix(user_context=user_context)}"
+                                f"/{entity_type}/{entity['id']}"
+                            ),
                             "path": f"/{entity_type}/{entity['id']}",
                             "class": "Directory",
                         }
@@ -672,12 +714,13 @@ def validate_and_register_entity(item, mapping: Dict[int, dict]) -> Literal[True
         if timeout:
             raise aiohttp.ServerTimeoutError
 
-    @staticmethod
     async def _yield_attachments(
+        self,
         entity_type: str,
         entity_id: str,
         endpoint: ParseResult,
         session: aiohttp.ClientSession,
+        user_context: OptionalUserContext = None,
     ) -> AsyncIterator[eLabFTWRemoteEntryWrapper[RemoteFile]]:
         """List attachments of a specific entity, e.g. "/resources/48"."""
         url = urljoin(
@@ -712,7 +755,10 @@ async def _yield_attachments(
                 RemoteFile(
                     **{
                         "name": upload["real_name"],
-                        "uri": f"elabftw://{endpoint.netloc}/{entity_type}/{entity_id}/{upload['id']}",
+                        "uri": (
+                            f"{self.get_scheme()}://{self.get_prefix(user_context=user_context)}"
+                            f"/{entity_type}/{entity_id}/{upload['id']}"
+                        ),
                         "path": f"/{entity_type}/{entity_id}/{upload['id']}",
                         "class": "File",
                         "size": upload["filesize"],
diff --git a/lib/galaxy/files/templates/examples/production_elabftw.yaml b/lib/galaxy/files/templates/examples/production_elabftw.yaml
new file mode 100644
index 000000000000..4523466e8927
--- /dev/null
+++ b/lib/galaxy/files/templates/examples/production_elabftw.yaml
@@ -0,0 +1,35 @@
+- id: elabftw
+  version: 0
+  name: eLabFTW
+  description: |
+    eLabFTW is a free and open source electronic lab notebook from Deltablot. It can keep track of experiments,
+    equipment, and materials from a research lab. Each lab can either host their own installation or go for Deltablot's
+    hosted solution. This template configuration allows you to connect to an eLabFTW instance of your choice.
+  variables:
+    endpoint:
+      label: eLabFTW instance endpoint (e.g. https://demo.elabftw.net)
+      type: string
+      help: |
+        The endpoint of the eLabFTW server you are connecting to. This should be the full URL including the protocol
+        (http or https) and the domain name.
+    writable:
+      label: Allow Galaxy to export data to eLabFTW?
+      type: boolean
+      default: true
+      help: |
+        Allow Galaxy to write data to this eLabFTW instance. Set it to "Yes" if you want to export data from Galaxy to
+        eLabFTW, set it to "No" if you only need to import data from eLabFTW to Galaxy. Keep in mind that your API key
+        must have matching permissions.
+  secrets:
+    api_key:
+      label: API Key
+      help: |
+        The API key to use to connect to the eLabFTW server. Navigate to the _Settings_ page on your eLabFTW server and
+        go to the _API Keys_ tab to generate a new key. Choose "Read/Write" permissions to enable both importing and
+        exporting data. "Read Only" API keys still work for importing data to Galaxy, but they will cause Galaxy to
+        error out when exporting data to eLabFTW.
+  configuration:
+    type: elabftw
+    endpoint: "{{ variables.endpoint }}"
+    api_key: "{{ secrets.api_key }}"
+    writable: "{{ variables.writable }}"
diff --git a/lib/galaxy/files/templates/models.py b/lib/galaxy/files/templates/models.py
index 967d83ea0894..6752f0e4d5ea 100644
--- a/lib/galaxy/files/templates/models.py
+++ b/lib/galaxy/files/templates/models.py
@@ -30,7 +30,9 @@
     UserDetailsDict,
 )
 
-FileSourceTemplateType = Literal["ftp", "posix", "s3fs", "azure", "onedata", "webdav", "dropbox", "googledrive"]
+FileSourceTemplateType = Literal[
+    "ftp", "posix", "s3fs", "azure", "onedata", "webdav", "dropbox", "googledrive", "elabftw"
+]
 
 
 class PosixFileSourceTemplateConfiguration(StrictModel):
@@ -195,6 +197,22 @@ class WebdavFileSourceConfiguration(StrictModel):
     writable: bool = False
 
 
+class eLabFTWFileSourceTemplateConfiguration(StrictModel):  # noqa
+    type: Literal["elabftw"]
+    endpoint: Union[str, TemplateExpansion]
+    api_key: Union[str, TemplateExpansion]
+    writable: Union[bool, TemplateExpansion] = True
+    template_start: Optional[str] = None
+    template_end: Optional[str] = None
+
+
+class eLabFTWFileSourceConfiguration(StrictModel):  # noqa
+    type: Literal["elabftw"]
+    endpoint: str
+    api_key: str
+    writable: bool = True
+
+
 FileSourceTemplateConfiguration = Union[
     PosixFileSourceTemplateConfiguration,
     S3FSFileSourceTemplateConfiguration,
@@ -204,6 +222,7 @@ class WebdavFileSourceConfiguration(StrictModel):
     WebdavFileSourceTemplateConfiguration,
     DropboxFileSourceTemplateConfiguration,
     GoogleDriveFileSourceTemplateConfiguration,
+    eLabFTWFileSourceTemplateConfiguration,
 ]
 FileSourceConfiguration = Union[
     PosixFileSourceConfiguration,
@@ -214,6 +233,7 @@ class WebdavFileSourceConfiguration(StrictModel):
     WebdavFileSourceConfiguration,
     DropboxFileSourceConfiguration,
     GoogleDriveFileSourceConfiguration,
+    eLabFTWFileSourceConfiguration,
 ]
 
 
@@ -284,6 +304,7 @@ def template_to_configuration(
     "webdav": WebdavFileSourceConfiguration,
     "dropbox": DropboxFileSourceConfiguration,
     "googledrive": GoogleDriveFileSourceConfiguration,
+    "elabftw": eLabFTWFileSourceConfiguration,
 }