Less common options for the pipeline, typically set in a config file.
Parameter |
Description |
Type |
Default |
Required |
Hidden |
publish_dir_mode |
Method used to save pipeline results to output directory. HelpThe Nextflow publishDir option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what |
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Parameter |
Description |
Type |
Default |
Required |
Hidden |
samplesheet |
samplesheet |
string |
'' |
True |
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enable_conda |
use conda (not supported) |
boolean |
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collect |
combine reads from multiple fastq files into one? |
boolean |
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skip_flye |
skip flye assembly |
boolean |
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skip_alignments |
skip alignment to initial assembly and reference |
boolean |
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flye_mode |
flye mode to use |
string |
'--nano-hq' |
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flye_args |
extra args for flye |
string |
'' |
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polish_pilon |
use pilon to polish with short reads |
boolean |
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medaka_model |
medaka model to use |
string |
'[email protected]:consesus' |
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lift_annotations |
run liftoff to lift over annotations |
boolean |
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out |
results directory |
string |
'./out' |
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scaffold_ragtag |
Scaffold with ragtag |
boolean |
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scaffold_links |
Scaffold with links |
boolean |
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scaffold_longstitch |
Scaffold with longstitch |
boolean |
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busoc_db |
path to busco db |
string |
/dss/dsslegfs01/pn73so/pn73so-dss-0000/becker_common/software/busco_db |
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porechop |
run porechop |
boolean |
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use_ref |
Use a reference genome |
boolean |
True |
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busco_lineage |
busco lineage to use |
string |
"brassicales_odb10" |
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jelly_is_reads |
not used |
string |
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True |
kmer_length |
k-mer length |
integer |
21 |
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read_length |
read length (if null, estimate) |
integer |
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dump |
Dump kmers from jellyfish |
boolean |
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genome_size |
Expected genome size, if null estimate from kmers |
integer |
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hifi |
HiFi reads available? |
boolean |
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lima |
Use lima on hifi reads |
boolean |
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pacbio_primers |
File containing pacbio primers |
string |
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hifi_ont |
Merge hifi and ont reads |
boolean |
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hifi_only |
Only hifi reads |
boolean |
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hifi_args |
Extra arguments for hifiasm |
string |
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polish_medaka |
polish with medaka |
string |
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