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. pipeline parameters

Generic options

Less common options for the pipeline, typically set in a config file.

Parameter Description Type Default Required Hidden
publish_dir_mode Method used to save pipeline results to output directory.
HelpThe Nextflow publishDir option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what

Other parameters

Parameter Description Type Default Required Hidden
samplesheet samplesheet string '' True
enable_conda use conda (not supported) boolean
collect combine reads from multiple fastq files into one? boolean
skip_flye skip flye assembly boolean
skip_alignments skip alignment to initial assembly and reference boolean
flye_mode flye mode to use string '--nano-hq'
flye_args extra args for flye string ''
polish_pilon use pilon to polish with short reads boolean
medaka_model medaka model to use string '[email protected]:consesus'
lift_annotations run liftoff to lift over annotations boolean
out results directory string './out'
scaffold_ragtag Scaffold with ragtag boolean
scaffold_links Scaffold with links boolean
scaffold_longstitch Scaffold with longstitch boolean
busoc_db path to busco db string /dss/dsslegfs01/pn73so/pn73so-dss-0000/becker_common/software/busco_db
porechop run porechop boolean
use_ref Use a reference genome boolean True
busco_lineage busco lineage to use string "brassicales_odb10"
jelly_is_reads not used string True
kmer_length k-mer length integer 21
read_length read length (if null, estimate) integer
dump Dump kmers from jellyfish boolean
genome_size Expected genome size, if null estimate from kmers integer
hifi HiFi reads available? boolean
lima Use lima on hifi reads boolean
pacbio_primers File containing pacbio primers string
hifi_ont Merge hifi and ont reads boolean
hifi_only Only hifi reads boolean
hifi_args Extra arguments for hifiasm string
polish_medaka polish with medaka string