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image coordinate file is incomplete #8

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slaide opened this issue Mar 21, 2024 · 0 comments
Open

image coordinate file is incomplete #8

slaide opened this issue Mar 21, 2024 · 0 comments

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@slaide
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slaide commented Mar 21, 2024

the image coordinate file contains the column headers "i, j, k, x(mm), y(mm), z(um)", which is unclear and incomplete.
the x,y,z coordinates is the physical position relative to the top left corner of the plate, which is fine.
i,j,k are the site indices within the well (for x,y,z, respectively).

there are several issues here:

  1. the well where the image was taken is not present in this data. the only way to relate these coordinates to an image file is by knowing the order in which the images were acquired, which is the same order of entries in this table.
  2. these coordinates are per site, not even per image. images in different channels may be recorded at different z coordinates (through use of the channel offset functionality). the coordinates should therefore be entered per image, not per site.
  3. using i,j,k to indicate the position within the well is not intuitive. the columns should have clearer names, e.g. 'site x' instead of 'i', and then rename 'x (mm)' to 'plate x (mm)' for clearer distinction.
  4. there should be a timestamp present for each image (which is currently completely missing in this file). while the image files themselves have a timestamp associated, the time coordinate for an image may also be relevant for several applications, and the image file timestamp may not be preserved across file system transfers.
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