README.md

Intro to Bulk RNA-Seq Bioinformatics using Tufts University Galaxy Server

Description

This course is an introduction to RNA sequencing using Galaxy on Tufts University's Galaxy server. The workshop is developed by the Data Intensive Studies Center (DISC) and Tufts Research Technology. For Galaxy Support, see the Galaxy documentation or email TTS Research Technology [email protected].

Goals

Bioinformatics for RNAseq has the following workflow steps

Materials Needed

• Chrome web browser
• Account on Tufts High Performance Compute Cluster
• VPN if accessing the HPC from off campus

Table of Contents

• Introduction Slides
• Introduction to Galaxy
• Next: Process Raw Reads
• Read Alignment
• Gene quantification
• Differential Expression

Acknowledgement

Thanks to bioinformatics intern Meg Farely for help developing this workshop. Much of this explanation has been adapted from these two sources:

• Harvard Chan Bioinformatics Core RNAseq training
• DESeq2 Vignette