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drtaPlotBrowseTraces.m
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function handles=drtaPlotBrowseTraces(handles)
% draqPlotSpikePreview(handles)
%
% Plots preview for spike trains
%
%
persistent auto_sign;
if isempty(auto_sign)
auto_sign=1;
end
do_notch60=1;
%Location of plots
left_axis=0.17;
right_axis=0.78;
bottom_offset=0.145;
height_delta=0.782;
figure(handles.w.drtaBrowseTraces);
set(gcf,'doublebuffer','on') %Reduce plot flicker
scaling = handles.draq_p.scaling;
offset = handles.draq_p.offset;
noch=handles.draq_p.no_spike_ch;
samp_bef=floor(handles.draq_p.ActualRate*handles.p.dt_pre_snip);
samp_aft=floor(handles.draq_p.ActualRate*handles.p.dt_post_snip);
data=drtaGetTraceData(handles);
%First determine whether this is hit, cr, etc
digi=[];
digi = data(:,handles.draq_p.no_chans);
%Please note that bit 1 is not used for spm because it is used in different
%programs for something else such as splus=1 sminus=0
try
shiftdata30=bitand(digi,2+4+8+16);
shift_dropc_nsampler=bitand(digi,1+2+4+8+16+32);
%Please note that there are problems with the start of the trial.
%Because of this we start looking 2 sec and beyond
shiftdata30(1:handles.draq_p.ActualRate*handles.p.exclude_secs)=0;
% shift_dropc_nsampler(1:handles.draq_p.ActualRate*handles.p.exclude_secs)=0;
odor_on=[];
switch handles.p.which_protocol
case {1,6}
%dropcspm
odor_on=find(shiftdata30==18,1,'first');
case 5
%dropcspm conc
t_start=find(shift_dropc_nsampler==1,1,'first');
if (sum((shift_dropc_nsampler>=2)&(shift_dropc_nsampler<=7))>2.4*handles.draq_p.ActualRate)&...
~isempty(find((shift_dropc_nsampler(t_start:end)>=2)&(shift_dropc_nsampler(t_start:end)<=7),1,'first'))
% odor_on=find((shift_dropc_nsampler>=2)&(shift_dropc_nsampler<=7),1,'first');
odor_on=t_start+find((shift_dropc_nsampler(t_start:end)>=2)&(shift_dropc_nsampler(t_start:end)<=7),1,'first')-1;
end
end
% try
% close(1)
% catch
% end
% hFig=figure(1);
% set(hFig, 'units','normalized','position',[.2 .4 .7 .25])
% plot(shiftdata30)
%Hit
if sum(shiftdata30==8)>0.05*handles.draq_p.ActualRate
drtaBrowseTraces('setTrialsOutcome',handles.w.drtaBrowseTraces,'Hit ');
%set(handles.trialOutcome,'String','Hit ');
else
%Miss
if sum(shiftdata30==10)>0.05*handles.draq_p.ActualRate
drtaBrowseTraces('setTrialsOutcome',handles.w.drtaBrowseTraces,'Miss');
%set(handles.trialOutcome,'String','Miss');
else
%CR
if sum(shiftdata30==12)>0.05*handles.draq_p.ActualRate
drtaBrowseTraces('setTrialsOutcome',handles.w.drtaBrowseTraces,'CR ');
%set(handles.trialOutcome,'String','CR ');
else
%FA
if sum(shiftdata30==14)>0.05*handles.draq_p.ActualRate
drtaBrowseTraces('setTrialsOutcome',handles.w.drtaBrowseTraces,'FA ');
else
%Short
if(length(find(shiftdata30>=1,1,'first'))==1)&(length(find(shiftdata30==8,1,'first'))~=1)&(length(find(shiftdata30==10,1,'first'))~=1)...
(length(find(shiftdata30==12,1,'first'))~=1)&(length(find(shiftdata30>0))>handles.draq_p.ActualRate*0.75)
drtaBrowseTraces('setTrialsOutcome',handles.w.drtaBrowseTraces,'Short');
else
drtaBrowseTraces('setTrialsOutcome',handles.w.drtaBrowseTraces,'Inter');
end
end
end
end
end
catch
end
if (handles.p.whichPlot~=11)
%Display analog records
if (handles.p.which_display==1)
%Display all traces
if do_notch60==1
notch60HzFilt = designfilt('bandstopiir','FilterOrder',2, ...
'HalfPowerFrequency1',59,'HalfPowerFrequency2',61, ...
'DesignMethod','butter','SampleRate',floor(handles.draq_p.ActualRate));
data=filtfilt(notch60HzFilt,data);
end
%Now proceed to plot all channels
switch handles.p.whichPlot
case 1
%Raw data
data1=data;
% case 2
% %Raw data -mean
% szdata=size(data);
% baseline=zeros(szdata(1),szdata(2));
% mean_data=mean(data,1);
% baseline=repmat(mean_data,szdata(1),1);
% data1=data-baseline;
case {2,3,4,5,6,7,8,9,10}
%Filter with different bandwidths
switch handles.p.whichPlot
case 2
fpass=[1 100];
case 3 %High Theta 6-10
fpass=[6 14];
case 4 %Theta 2-12
fpass=[1 4];
case 5 %Beta 15-36
fpass=[15 30];
case 6 %Gamma1 35-65
fpass=[35 45];
case 7 %Gamma2 65-95
fpass=[65 95];
case 8 %Gamma 35-95
fpass=[35 95];
case {9,10} %Spikes 500-5000
fpass=[500 5000];
end
% 20th order filter
bpFilt = designfilt('bandpassiir','FilterOrder',20, ...
'HalfPowerFrequency1',fpass(1),'HalfPowerFrequency2',fpass(2), ...
'SampleRate',floor(handles.draq_p.ActualRate));
data1=filtfilt(bpFilt,data);
%From wave_clus amp_detect
% sr=floor(handles.draq_p.ActualRate);
% [b,a]=ellip(2,0.1,40,[fpass(1) fpass(2)]*2/sr);
% data1=filtfilt(b,a,data);
if handles.p.whichPlot==10
%Calculate the moving variance
data1=movvar(data1,ceil(0.05*handles.draq_p.ActualRate));
end
end
if (handles.p.doSubtract==1)
data2=data1;
for tetr=1:4
for jj=1:4
if handles.p.subtractCh(4*(tetr-1)+jj)<=18
if handles.p.subtractCh(4*(tetr-1)+jj)<=16
%Subtract one of the channels
data1(:,(tetr-1)*4+jj)=data2(:,(tetr-1)*4+jj)-data2(:,handles.p.subtractCh((tetr-1)*4+jj));
else
if handles.p.subtractCh(4*(tetr-1)+jj)==17
%Subtract tetrode mean
data1(:,(tetr-1)*4+jj)=data2(:,(tetr-1)*4+jj)-mean(data2(:,(tetr-1)*4+1:(tetr-1)*4+4),2);
else
%Subtract average of all electrodes
data1(:,(tetr-1)*4+jj)=data2(:,(tetr-1)*4+jj)-mean(data2,2);
end
end
end
end
end
end
CHID = [1:16];
for (ii=1:noch)
bottom=bottom_offset+(0.80/noch)*(ii-0.5);
height=height_delta/noch;
s_handle(ii)=subplot('Position', [left_axis bottom right_axis height]);
ii_from=floor((handles.draq_p.acquire_display_start+handles.p.start_display_time)...
*handles.draq_p.ActualRate+1);
ii_to=floor((handles.draq_p.acquire_display_start+handles.p.start_display_time...
+handles.p.display_interval)*handles.draq_p.ActualRate);
plot(data1(ii_from:ii_to,CHID(ii)));
sz_dat=length(data1);
tim=[0 sz_dat];
%Calculate 2.5 SD
%Now plot 3xmedian(std)
datavec=data1(:,CHID(ii));
sdvec=zeros(1,ceil(length(datavec)/1000));
jj=0;
for kk=1:1000:length(datavec)-1000
jj=jj+1;
sdvec(jj)=std(datavec(kk:kk+1000));
end
%Set threshold to 2.5 or -2.5 xSD
two_half_med_SD=2.5*median(sdvec);
%If this is zero this is the differentially subtracted
%channel, set 2.5SD high
if two_half_med_SD==0
two_half_med_SD=100;
end
if handles.p.set2p5SD==1
handles.p.threshold(ii)=two_half_med_SD;
end
if handles.p.setm2p5SD==1
handles.p.threshold(ii)=-two_half_med_SD;
end
%Set threshold to nxSD
nxSD=handles.p.nxSD*median(sdvec);
%If nxSD=0 this is the differentially subtracted channel
if nxSD==0
nxSD=1000;
handles.p.threshold(ii)=nxSD;
end
if handles.p.setnxSD==1
handles.p.threshold(ii)=nxSD;
end
%Set threshold to uv
if handles.p.setThr==1
handles.p.threshold(ii)=handles.p.thrToSet;
end
hold on
plot(tim,[handles.p.threshold(ii) handles.p.threshold(ii)],'r');
if exist('odor_on')~=0
if ~isempty(odor_on)
plot([odor_on odor_on],[-handles.draq_p.prev_ylim(ii) handles.draq_p.prev_ylim(ii)],'r');
end
end
drtaThresholdSnips('update_p',handles.w.drtaThresholdSnips,handles);
hold off
ylim(s_handle(ii),[-handles.draq_p.prev_ylim(ii) handles.draq_p.prev_ylim(ii)]);
set(gca,'YTick',[-handles.draq_p.prev_ylim(ii)+(handles.draq_p.prev_ylim(ii)/3) handles.draq_p.prev_ylim(ii)-(handles.draq_p.prev_ylim(ii)/3)]);
tick_label={};
tick_label{1}=num2str(floor(-2*handles.draq_p.prev_ylim(ii)/3));
tick_label{2}=num2str(floor(2*handles.draq_p.prev_ylim(ii)/3));
set(gca,'YTickLabel',tick_label);
xlim(s_handle(ii),[1 1+handles.p.display_interval*handles.draq_p.ActualRate]);
ylabel(s_handle(ii),num2str(ii));
if ii==1
%xlabel('Time (sec)');
dt=handles.p.display_interval/5;
dt=round(dt*10^(-floor(log10(dt))))/10^(-floor(log10(dt)));
d_samples=dt*handles.draq_p.ActualRate;
set(gca,'XTick',0:d_samples:handles.p.display_interval*handles.draq_p.ActualRate);
time=handles.p.start_display_time;
jj=1;
while time<(handles.p.start_display_time+handles.p.display_interval)
tick_label{jj}=num2str(time);
time=time+dt;
jj=jj+1;
end
tick_label{jj}=num2str(time);
set(gca,'XTickLabel',tick_label);
else
set(gca,'XTick',0:d_samples:handles.p.display_interval*handles.draq_p.ActualRate);
set(gca,'XTickLabel','');
end
end
else
%Display only one trace
% try
% close 10
% catch
% end
%
% figure(10)
ii=handles.p.which_display-1;
bottom=bottom_offset+(0.80/noch)*(1-0.5);
height=height_delta;
s_handle(ii)=subplot('Position', [left_axis bottom right_axis height]);
% notch60HzFilt = designfilt('bandstopiir','FilterOrder',2, ...
% 'HalfPowerFrequency1',59,'HalfPowerFrequency2',61, ...
% 'DesignMethod','butter','SampleRate',floor(handles.draq_p.ActualRate));
if do_notch60==1
notch60HzFilt = designfilt('bandstopiir','FilterOrder',2, ...
'HalfPowerFrequency1',59,'HalfPowerFrequency2',61, ...
'DesignMethod','butter','SampleRate',floor(handles.draq_p.ActualRate));
data=filtfilt(notch60HzFilt,data);
end
%Now proceed to plot this channel
switch handles.p.whichPlot
case 2
fpass=[1 100];
case 3 %High Theta 6-10
fpass=[6 14];
case 4 %Theta 2-12
fpass=[1 4];
case 5 %Beta 15-36
fpass=[15 30];
case 6 %Gamma1 35-65
fpass=[35 45];
case 7 %Gamma2 65-95
fpass=[65 95];
case 8 %Gamma 35-95
fpass=[35 95];
case {9,10} %Spikes 500-5000
fpass=[500 5000];
end
if handles.p.whichPlot==1
%Raw data
data1=data;
else
%20th order filter
bpFilt = designfilt('bandpassiir','FilterOrder',20, ...
'HalfPowerFrequency1',fpass(1),'HalfPowerFrequency2',fpass(2), ...
'SampleRate',floor(handles.draq_p.ActualRate));
data1=filtfilt(bpFilt,data);
% %From erpimage
% %bandpass filter the data 3rd order
% [B A]=butter(3,fpass*2/floor(handles.draq_p.ActualRate));
% data1=filtfilt(B,A,data);
%From wave_clus amp_detect
% sr=floor(handles.draq_p.ActualRate);
% [b,a]=ellip(2,0.1,40,[fpass(1) fpass(2)]*2/sr);
% data1=filtfilt(b,a,data);
if handles.p.whichPlot==10
%Calculate the moving variance
data1=movvar(data1,ceil(0.05*handles.draq_p.ActualRate));
end
end
if (handles.p.doSubtract==1)
data2=data1;
for tetr=1:4
for jj=1:4
if handles.p.subtractCh(4*(tetr-1)+jj)<=18
if handles.p.subtractCh(4*(tetr-1)+jj)<=16
%Subtract one of the channels
data1(:,(tetr-1)*4+jj)=data2(:,(tetr-1)*4+jj)-data2(:,handles.p.subtractCh((tetr-1)*4+jj));
else
if handles.p.subtractCh(4*(tetr-1)+jj)==17
%Subtract tetrode mean
data1(:,(tetr-1)*4+jj)=data2(:,(tetr-1)*4+jj)-mean(data2(:,(tetr-1)*4+1:(tetr-1)*4+4),2);
else
%Subtract average of all electrodes
data1(:,(tetr-1)*4+jj)=data2(:,(tetr-1)*4+jj)-mean(data2,2);
end
end
end
end
end
end
CHID = [1:16];
ii_from=floor((handles.draq_p.acquire_display_start+handles.p.start_display_time)...
*handles.draq_p.ActualRate+1);
ii_to=floor((handles.draq_p.acquire_display_start+handles.p.start_display_time...
+handles.p.display_interval)*handles.draq_p.ActualRate);
%plot(data2(ii_from:ii_to,CHID(ii)));
hold off
plot(data1(ii_from:ii_to,CHID(ii)),'-b');
hold on
% Commented out plot the trace to use in a figure for publication
% figure(1)
% time=(1:length(data1(ii_from:ii_to,CHID(ii))))/handles.draq_p.ActualRate;
% plot(time,data1(ii_from:ii_to,CHID(ii)),'-b');
% ylim([-2500 2500])
%This is equation 3.1 of Quiroga et al Neural Comp 16:1661 (2004)
% quiroga_thr=4*median(abs(data1(ii_from:ii_to,CHID(ii)))/0.6745);
% plot([ii_from ii_to], [quiroga_thr quiroga_thr],'-c');
% plot([ii_from ii_to], [-quiroga_thr -quiroga_thr],'-c');
%Now plot 3xmedian(std)
datavec=data1(:,CHID(ii));
sdvec=zeros(1,ceil(length(datavec)/1000));
jj=0;
for kk=1:1000:length(datavec)-1000
jj=jj+1;
sdvec(jj)=std(datavec(kk:kk+1000));
end
two_half_med_SD=2.5*median(sdvec);
plot([1 ii_to-ii_from+1],[two_half_med_SD two_half_med_SD],'-c');
hold on
plot([1 ii_to-ii_from+1],[-two_half_med_SD -two_half_med_SD],'-c');
three_t_med_SD=3*median(sdvec);
plot([1 ii_to-ii_from+1],[three_t_med_SD three_t_med_SD],'-y');
hold on
plot([1 ii_to-ii_from+1],[-three_t_med_SD -three_t_med_SD],'-y');
sz_dat=length(data1);
tim=[0 sz_dat(1)];
hold on
if isfield(handles.p,'last_threshold')
plot(tim,[handles.p.last_threshold(ii) handles.p.last_threshold(ii)],'y');
end
%Set threshold if requested by user
%If this is zero this is the differentially subtracted
%channel, set 2.5SD high
if two_half_med_SD==0
two_half_med_SD=100;
end
if handles.p.set2p5SD==1
handles.p.threshold(ii)=two_half_med_SD;
end
if handles.p.setm2p5SD==1
handles.p.threshold(ii)=-two_half_med_SD;
end
%Set threshold to nxSD
nxSD=handles.p.nxSD*median(sdvec);
%If nxSD=0 this is the differentially subtracted channel
if nxSD==0
nxSD=1000;
handles.p.threshold(ii)=nxSD;
end
if handles.p.setnxSD==1
handles.p.threshold(ii)=nxSD;
end
%Set threshold to uv
if handles.p.setThr==1
handles.p.threshold(ii)=handles.p.thrToSet;
end
drtaThresholdSnips('update_p',handles.w.drtaThresholdSnips,handles);
plot(tim,[handles.p.threshold(ii) handles.p.threshold(ii)],'r');
thr=handles.p.threshold(ii)
if exist('odor_on')~=0
if ~isempty(odor_on)
plot([odor_on odor_on],[-handles.draq_p.prev_ylim(ii) handles.draq_p.prev_ylim(ii)],'r');
end
end
hleg1 = legend('Voltage','2.5xSD','2.5xSD','3xSD','3xSD','Threshold','Threshold','Discard');
hold off
% if (0)
% hold on
% data_0 = filtfilt(b,1,data( floor((handles.draq_p.acquire_display_start+handles.p.start_display_time)*handles.draq_p.ActualRate+1):...
% floor((handles.draq_p.acquire_display_start+handles.p.start_display_time+handles.p.display_interval)*handles.draq_p.ActualRate),ii));
% plot(data_0);
% hold off
% end
ylim(s_handle(ii),[-handles.draq_p.prev_ylim(ii) handles.draq_p.prev_ylim(ii)]);
set(gca,'YTick',[-handles.draq_p.prev_ylim(ii)+(handles.draq_p.prev_ylim(ii)/3) handles.draq_p.prev_ylim(ii)-(handles.draq_p.prev_ylim(ii)/3)]);
tick_label={};
tick_label{1}=num2str(floor(-2*handles.draq_p.prev_ylim(ii)/3));
tick_label{2}=num2str(floor(2*handles.draq_p.prev_ylim(ii)/3));
set(gca,'YTickLabel',tick_label);
xlim(s_handle(ii),[1 1+handles.p.display_interval*handles.draq_p.ActualRate]);
ylabel(s_handle(ii),num2str(ii));
xlabel('Time (sec)');
dt=handles.p.display_interval/5;
dt=round(dt*10^(-floor(log10(dt))))/10^(-floor(log10(dt)));
d_samples=dt*handles.draq_p.ActualRate;
set(gca,'XTick',0:d_samples:handles.p.display_interval*handles.draq_p.ActualRate);
time=handles.p.start_display_time;
jj=1;
while time<(handles.p.start_display_time+handles.p.display_interval)
tick_label{jj}=num2str(time);
time=time+dt;
jj=jj+1;
end
tick_label{jj}=num2str(time);
set(gca,'XTickLabel',tick_label);
%Stop here if you want to save the figure
pffft=1;
end
else
%This will plot the non-spike traces (traces reporting on
%digital values, sniffing, etc
drtaShowDigital(handles);
end
function [p_handle s_handle]= drtaSetupBrowse(handles)
%Setup the plots
d_samples=0;
noch=handles.draq_p.no_spike_ch;
for (ii=1:noch)
bottom=bottom_offset+(0.80/noch)*(ii-0.5);
height=height_delta/noch;
s_handle(ii)=subplot('Position', [left_axis bottom right_axis height]);
p_handle(ii)= plot(zeros(handles.p.display_interval*handles.draq_p.ActualRate,1));
v_max=(handles.draq_p.prev_ylim(ii)*handles.draq_p.pre_gain*handles.draq_p.daq_gain/1000000);
v_min=-(handles.draq_p.prev_ylim(ii)*handles.draq_p.pre_gain*handles.draq_p.daq_gain/1000000);
scaling = handles.draq_p.scaling;
offset = handles.draq_p.offset;
nat_max=(v_max-offset)/scaling;
nat_min=(v_min-offset)/scaling;
ylim(s_handle(ii),[nat_min nat_max]); %Note numbers are native
set(gca,'YTick',[( ((nat_max+nat_min)/2)-((nat_max-nat_min)/3) ) (nat_max+nat_min)/2 ( ((nat_max+nat_min)/2)+ ((nat_max-nat_min)/3) )]);
tick_label{1}=num2str(floor(-2*handles.draq_p.prev_ylim(ii)/3));
tick_label{2}='';
tick_label{3}=num2str(floor(2*handles.draq_p.prev_ylim(ii)/3));
set(gca,'YTickLabel',tick_label);
xlim(s_handle(ii),[1 1+handles.p.display_interval*handles.draq_p.ActualRate]);
ylabel(s_handle(ii),num2str(ii));
if ii==1
xlabel('Time (sec)');
dt=handles.p.display_interval/5;
dt=round(dt*10^(-floor(log10(dt))))/10^(-floor(log10(dt)));
d_samples=dt*handles.draq_p.ActualRate;
set(gca,'XTick',0:d_samples:handles.p.display_interval*handles.draq_p.ActualRate);
time=handles.p.start_display_time;
jj=1;
while time<(handles.p.start_display_time+handles.p.display_interval)
tick_label{jj}=num2str(time);
time=time+dt;
jj=jj+1;
end
tick_label{jj}=num2str(time);
set(gca,'XTickLabel',tick_label);
else
set(gca,'XTick',0:d_samples:handles.p.display_interval*handles.draq_p.ActualRate);
set(gca,'XTickLabel','');
end
end