Human kidney reference

[WesDe]

Hi,

I have seen that you have recently added human kidney reference from the recent biorxiv by Lake et al and that's great ! I have a couple of questions about this reference.

The pipeline used in the publication to produce the reference is based on home made normalization, home made batch effect correction and analysis with Pagoda2. I was wondering if it was compatible with the Map/Query method from Seurat ? The fact that the reference was obtained from another tool than Seurat will not impact the Map/Query result ?

If I follow the instruction from issue Map/Query, Do I need to preprocess my data with the same normalization method than the reference ?

Best,
WesDe