From fd1ea6eccb05cd2270874081bbff8772cff452f4 Mon Sep 17 00:00:00 2001 From: Peter Carbonetto Date: Wed, 13 Jan 2021 23:33:35 -0600 Subject: [PATCH 01/50] Working on FitTopicModel and fast_topics_demo example script. --- DESCRIPTION | 9 +++-- NAMESPACE | 3 ++ R/fasttopics.R | 81 +++++++++++++++++++++++++++++++++++++++++ docs/fast_topics_demo.R | 29 +++++++++++++++ docs/fasttopics.Rmd | 0 man/FitTopicModel.Rd | 46 +++++++++++++++++++++++ man/RunQuantileNorm.Rd | 4 +- 7 files changed, 167 insertions(+), 5 deletions(-) create mode 100644 R/fasttopics.R create mode 100644 docs/fast_topics_demo.R create mode 100644 docs/fasttopics.Rmd create mode 100644 man/FitTopicModel.Rd diff --git a/DESCRIPTION b/DESCRIPTION index cb24822..4e5b7a7 100644 --- a/DESCRIPTION +++ b/DESCRIPTION @@ -1,7 +1,7 @@ Package: SeuratWrappers Title: Community-Provided Methods and Extensions for the Seurat Object -Version: 0.3.0 -Date: 2020-09-29 +Version: 0.3.1 +Date: 2021-01-11 Authors@R: c( person(given = 'Rahul', family = 'Satija', email = 'rsatija@nygenome.org', role = 'aut', comment = c(ORCID = '0000-0001-9448-8833')), person(given = 'Andrew', family = 'Butler', email = 'abutler@nygenome.org', role = 'aut', comment = c(ORCID = '0000-0003-3608-0463')), @@ -22,7 +22,8 @@ Remotes: MacoskoLab/liger, SaskiaFreytag/schex@031320d, cole-trapnell-lab/monocle3, mojaveazure/seurat-disk, - powellgenomicslab/Nebulosa + powellgenomicslab/Nebulosa, + stephenslab/fastTopics Depends: R (>= 3.5.0) biocViews: @@ -46,6 +47,7 @@ Collate: 'cogaps.R' 'conos.R' 'fast_mnn.R' + 'fasttopics.R' 'glmpca.R' 'liger.R' 'monocle3.R' @@ -67,6 +69,7 @@ Suggests: fishpond, monocle3, CoGAPS, + fastTopics, glmpca, Nebulosa, presto diff --git a/NAMESPACE b/NAMESPACE index f52b8f4..53101f0 100644 --- a/NAMESPACE +++ b/NAMESPACE @@ -7,6 +7,7 @@ S3method(as.Seurat,cell_data_set) S3method(as.Seurat,list) S3method(as.cell_data_set,Seurat) export(ALRAChooseKPlot) +export(FitTopicModel) export(ReadAlevin) export(ReadVelocity) export(RunALRA) @@ -23,6 +24,7 @@ export(RunVelocity) export(as.cell_data_set) importFrom(BiocManager,install) importFrom(Matrix,Matrix) +importFrom(Matrix,t) importFrom(Matrix,writeMM) importFrom(Seurat,"DefaultAssay<-") importFrom(Seurat,"Idents<-") @@ -71,6 +73,7 @@ importFrom(rlang,duplicate) importFrom(rsvd,rsvd) importFrom(stats,as.dist) importFrom(stats,pnorm) +importFrom(stats,prcomp) importFrom(stats,quantile) importFrom(stats,sd) importFrom(stats,setNames) diff --git a/R/fasttopics.R b/R/fasttopics.R new file mode 100644 index 0000000..ef1687f --- /dev/null +++ b/R/fasttopics.R @@ -0,0 +1,81 @@ +#' @title Add Title Here +#' +#' @description Add description here. +#' +#' @param object Describe input argument "object" here. +#' +#' @param k Describe input argument "k" here. +#' +#' @param assay Describe input argument "assay" here. +#' +#' @param features Describe input argument "features" here. +#' +#' @param reduction.name Describe input argument "reduction.name" here. +#' +#' @param reduction.key Describe input argument "reduction.key" here. +#' +#' @param verbose Describe input argument "verbose" here. +#' +#' @param \dots Describe "..." here. +#' +#' @return Describe the return value here. +#' +#' Clarify that, unfortunately, "factors" and "loadings" in fastTopics +#' mean the opposite of what they mean in Seurat. +#' +#' @seealso \code{\link[fastTopics]{fit_topic_model}} +#' +#' @importFrom stats prcomp +#' @importFrom Matrix t +#' +#' @export +#' +FitTopicModel <- function (object, k = 3, assay = NULL, features = NULL, + reduction.name = "multinom_topic_model", + reduction.key = "k_", verbose = TRUE, ...) { + + # Check the input arguments, and that fastTopics is installed. + CheckPackage(package = "stephenslab/fastTopics") + if (!inherits(object,"Seurat")) + stop("\"object\" must be a Seurat object",call. = FALSE) + + # Get the n x m counts matrix, where n is the number of samples + # (cells) and m is the number of selected genes. + assay <- assay %||% DefaultAssay(object) + DefaultAssay(object) <- assay + features <- features %||% VariableFeatures(object) + X <- GetAssayData(object,"counts") + if (length(features) == 0) + features <- rownames(X) + else + features <- intersect(features,rownames(X)) + X <- X[features,] + X <- t(X) + + # TO DO: Remove any columns that are all zeros. + + # Fit the multinomial topic model using fastTopics. + fit <- fit_topic_model(X,k,verbose = verbose,...) + class(fit) <- c("list","multinom_topic_model_fit") + + # Retrieve the factors matrix (n x k) and loadings matrix (m x k). + embeddings <- fit$L + loadings <- fit$F + colnames(embeddings) <- paste0(reduction.key,1:k) + colnames(loadings) <- paste0(reduction.key,1:k) + + # Add the topic model fit to the Seurat object. + object[[reduction.name]] <- + CreateDimReducObject(embeddings,loadings,assay = assay,key = reduction.key, + global = TRUE,misc = fit) + + # Add a PCA dimension reduction from the mixture proportions. + out <- prcomp(fit$L) + colnames(out$x) <- paste0("PC_",1:k) + colnames(out$rotation) <- paste0("PC_",1:k) + object[["pca"]] <- + CreateDimReducObject(out$x[,-k],out$rotation[,-k],assay = assay, + key = "PC_",global = TRUE) + + return(LogSeuratCommand(object)) +} diff --git a/docs/fast_topics_demo.R b/docs/fast_topics_demo.R new file mode 100644 index 0000000..df66eb5 --- /dev/null +++ b/docs/fast_topics_demo.R @@ -0,0 +1,29 @@ +# Load the packages used in the analysis below. +library(Seurat) +library(SeuratData) +library(SeuratWrappers) +library(fastTopics) + +# Set seed to generate results that are reproducible. + +# Load the UMI count data. +InstallData("pbmc3k") +data(pbmc3k) + +# No pre-processing is needed. + +# Fit topic model to UMI counts. Note that it may take several minutes +# for the model fitting to complete. +pbmc3k <- FitTopicModel(pbmc3k,k = 6,numiter.main = 10,numiter.refine = 10) + +# Plot the top two PCs of the mixture proportions. +Idents(pbmc3k) <- pbmc3k$seurat_annotations +DimPlot(pbmc3k) + +# Create a Structure plot. +fit <- Misc(Reductions(pbmc3k,"multinom_topic_model")) +p <- structure_plot(fit,grouping = Idents(pbmc3k),gap = 30) + +# Fit non-negative matrix factorization to UMI counts. +# TO DO. + diff --git a/docs/fasttopics.Rmd b/docs/fasttopics.Rmd new file mode 100644 index 0000000..e69de29 diff --git a/man/FitTopicModel.Rd b/man/FitTopicModel.Rd new file mode 100644 index 0000000..a1584ff --- /dev/null +++ b/man/FitTopicModel.Rd @@ -0,0 +1,46 @@ +% Generated by roxygen2: do not edit by hand +% Please edit documentation in R/fasttopics.R +\name{FitTopicModel} +\alias{FitTopicModel} +\title{Add Title Here} +\usage{ +FitTopicModel( + object, + k = 3, + assay = NULL, + features = NULL, + reduction.name = "fasttopics", + reduction.key = "k_", + verbose = TRUE, + ... +) +} +\arguments{ +\item{object}{Describe input argument "object" here.} + +\item{k}{Describe input argument "k" here.} + +\item{assay}{Describe input argument "assay" here.} + +\item{features}{Describe input argument "features" here.} + +\item{reduction.name}{Describe input argument "reduction.name" here.} + +\item{reduction.key}{Describe input argument "reduction.key" here.} + +\item{verbose}{Describe input argument "verbose" here.} + +\item{\dots}{Describe "..." here.} +} +\value{ +Describe the return value here. + +Clarify that, unfortunately, "factors" and "loadings" in fastTopics +mean the opposite of what they mean in Seurat. +} +\description{ +Add description here. +} +\seealso{ +\code{\link[fastTopics]{fit_topic_model}} +} diff --git a/man/RunQuantileNorm.Rd b/man/RunQuantileNorm.Rd index 7678e6d..0e1ca5a 100644 --- a/man/RunQuantileNorm.Rd +++ b/man/RunQuantileNorm.Rd @@ -47,10 +47,10 @@ the dataset with the largest number of cells is used.} \item{do.center}{Centers the data when scaling factors (useful for less sparse modalities like methylation data). (default FALSE)} -\item{max_sample}{Maximum number of cells used for quantile normalization of each cluster +\item{max_sample}{Maximum number of cells used for quantile normalization of each cluster and factor. (default 1000)} -\item{eps}{The error bound of the nearest neighbor search. (default 0.9) Lower values give more +\item{eps}{The error bound of the nearest neighbor search. (default 0.9) Lower values give more accurate nearest neighbor graphs but take much longer to computer.} \item{refine.knn}{whether to increase robustness of cluster assignments using KNN graph.(default TRUE)} From 8baf086aa6e7b9a9973f354b4f7b9d6fc0313ee5 Mon Sep 17 00:00:00 2001 From: Peter Carbonetto Date: Wed, 13 Jan 2021 23:43:26 -0600 Subject: [PATCH 02/50] Made a few small improvements to the fastTopics demo. --- docs/fast_topics_demo.R | 5 +++-- 1 file changed, 3 insertions(+), 2 deletions(-) diff --git a/docs/fast_topics_demo.R b/docs/fast_topics_demo.R index df66eb5..b233014 100644 --- a/docs/fast_topics_demo.R +++ b/docs/fast_topics_demo.R @@ -5,6 +5,7 @@ library(SeuratWrappers) library(fastTopics) # Set seed to generate results that are reproducible. +set.seed(1) # Load the UMI count data. InstallData("pbmc3k") @@ -14,7 +15,7 @@ data(pbmc3k) # Fit topic model to UMI counts. Note that it may take several minutes # for the model fitting to complete. -pbmc3k <- FitTopicModel(pbmc3k,k = 6,numiter.main = 10,numiter.refine = 10) +pbmc3k <- FitTopicModel(pbmc3k,k = 6) # Plot the top two PCs of the mixture proportions. Idents(pbmc3k) <- pbmc3k$seurat_annotations @@ -22,7 +23,7 @@ DimPlot(pbmc3k) # Create a Structure plot. fit <- Misc(Reductions(pbmc3k,"multinom_topic_model")) -p <- structure_plot(fit,grouping = Idents(pbmc3k),gap = 30) +p <- structure_plot(fit,grouping = Idents(pbmc3k),gap = 25) # Fit non-negative matrix factorization to UMI counts. # TO DO. From 4fb6cad689c993bf3646334fd0f139faa015d9d9 Mon Sep 17 00:00:00 2001 From: Peter Carbonetto Date: Thu, 14 Jan 2021 09:28:33 -0600 Subject: [PATCH 03/50] Filled out more of the roxygen2 docs for FitTopicModel. --- R/fasttopics.R | 65 ++++++++++++++++++++++++++++++++++---------- man/FitTopicModel.Rd | 59 +++++++++++++++++++++++++++++++++------- 2 files changed, 100 insertions(+), 24 deletions(-) diff --git a/R/fasttopics.R b/R/fasttopics.R index ef1687f..b082ca2 100644 --- a/R/fasttopics.R +++ b/R/fasttopics.R @@ -1,30 +1,67 @@ -#' @title Add Title Here +#' @title Fit a Multinomial Topic Model Using fastTopics #' #' @description Add description here. #' -#' @param object Describe input argument "object" here. +#' @param object A Seurat object. #' -#' @param k Describe input argument "k" here. +#' @param k The number of topics. Must be 2 or more. #' -#' @param assay Describe input argument "assay" here. +#' @param assay Name of assay to use; defaults to the default +#' assay of the object. #' -#' @param features Describe input argument "features" here. +#' @param features A list of features to use for fitting the model. If +#' \code{features = NULL}, all variable features; see +#' \code{\link[Seurat]{VariableFeatures}}. #' -#' @param reduction.name Describe input argument "reduction.name" here. +#' @param reduction.name Name of the outputted reduction. #' -#' @param reduction.key Describe input argument "reduction.key" here. +#' @param reduction.key Key for the outputted reduction. #' -#' @param verbose Describe input argument "verbose" here. +#' @param verbose When \code{verbose = TRUE}, information about the +#' progress of the model fitting is printed to the console. See +#' \code{\link[fastTopics]{fit_poisson_nmf}} for an explanation of the +#' output. #' -#' @param \dots Describe "..." here. +#' @param \dots \dots Additional arguments passed to +#' \code{fit_topic_model}; see +#' \code{\link[fastTopics]{fit_topic_model}} for details #' #' @return Describe the return value here. #' #' Clarify that, unfortunately, "factors" and "loadings" in fastTopics #' mean the opposite of what they mean in Seurat. -#' +#' +#' @author Peter Carbonetto +#' +#' @references +#' Dey, K. K., Hsiao, C. J. and Stephens, M. (2017). Visualizing the +#' structure of RNA-seq expression data using grade of membership +#' models. \emph{PLoS Genetics} \bold{13}, e1006599. +#' #' @seealso \code{\link[fastTopics]{fit_topic_model}} #' +#' @examples +#' set.seed(1) +#' +#' # Load the PBMC data. +#' data(pbmc_small) +#' +#' # Fit the multinomial topic model to the raw UMI count data; no +#' # pre-processing is needed. +#' pbmc_small <- FitTopicModel(pbmc_small,k = 3) +#' +#' # This plot shows the cells projected onto the top 2 principal +#' # components (PCs) of the topic model mixture proportions. +#' Idents(pbmc_small) <- pbmc_small$letter.idents +#' DimPlot(pbmc_small,reduction = "pca") +#' +#' # Once fitted topic model is extracted, many functions from the +#' # fastTopics package can be used. For example, the Structure plot +#' # provides an evocative visual summary of the estimated mixture +#' # proportions for each cell. +#' fit <- Misc(Reductions(pbmc_small,"multinom_topic_model")) +#' structure_plot(fit,grouping = Idents(pbmc_small),gap = 5) +#' #' @importFrom stats prcomp #' @importFrom Matrix t #' @@ -52,8 +89,6 @@ FitTopicModel <- function (object, k = 3, assay = NULL, features = NULL, X <- X[features,] X <- t(X) - # TO DO: Remove any columns that are all zeros. - # Fit the multinomial topic model using fastTopics. fit <- fit_topic_model(X,k,verbose = verbose,...) class(fit) <- c("list","multinom_topic_model_fit") @@ -69,13 +104,15 @@ FitTopicModel <- function (object, k = 3, assay = NULL, features = NULL, CreateDimReducObject(embeddings,loadings,assay = assay,key = reduction.key, global = TRUE,misc = fit) - # Add a PCA dimension reduction from the mixture proportions. + # Add a PCA dimension reduction calculated from the mixture + # proportions. out <- prcomp(fit$L) colnames(out$x) <- paste0("PC_",1:k) colnames(out$rotation) <- paste0("PC_",1:k) object[["pca"]] <- CreateDimReducObject(out$x[,-k],out$rotation[,-k],assay = assay, key = "PC_",global = TRUE) - + + # Output the updated Seurat object. return(LogSeuratCommand(object)) } diff --git a/man/FitTopicModel.Rd b/man/FitTopicModel.Rd index a1584ff..c9606a8 100644 --- a/man/FitTopicModel.Rd +++ b/man/FitTopicModel.Rd @@ -2,35 +2,43 @@ % Please edit documentation in R/fasttopics.R \name{FitTopicModel} \alias{FitTopicModel} -\title{Add Title Here} +\title{Fit a Multinomial Topic Model Using fastTopics} \usage{ FitTopicModel( object, k = 3, assay = NULL, features = NULL, - reduction.name = "fasttopics", + reduction.name = "multinom_topic_model", reduction.key = "k_", verbose = TRUE, ... ) } \arguments{ -\item{object}{Describe input argument "object" here.} +\item{object}{A Seurat object.} -\item{k}{Describe input argument "k" here.} +\item{k}{The number of topics. Must be 2 or more.} -\item{assay}{Describe input argument "assay" here.} +\item{assay}{Name of assay to use; defaults to the default +assay of the object.} -\item{features}{Describe input argument "features" here.} +\item{features}{A list of features to use for fitting the model. If +\code{features = NULL}, all variable features; see +\code{\link[Seurat]{VariableFeatures}}.} -\item{reduction.name}{Describe input argument "reduction.name" here.} +\item{reduction.name}{Name of the outputted reduction.} -\item{reduction.key}{Describe input argument "reduction.key" here.} +\item{reduction.key}{Key for the outputted reduction.} -\item{verbose}{Describe input argument "verbose" here.} +\item{verbose}{When \code{verbose = TRUE}, information about the +progress of the model fitting is printed to the console. See +\code{\link[fastTopics]{fit_poisson_nmf}} for an explanation of the +output.} -\item{\dots}{Describe "..." here.} +\item{\dots}{\dots Additional arguments passed to +\code{fit_topic_model}; see +\code{\link[fastTopics]{fit_topic_model}} for details} } \value{ Describe the return value here. @@ -41,6 +49,37 @@ mean the opposite of what they mean in Seurat. \description{ Add description here. } +\examples{ +set.seed(1) + +# Load the PBMC data. +data(pbmc_small) + +# Fit the multinomial topic model to the raw UMI count data; no +# pre-processing is needed. +pbmc_small <- FitTopicModel(pbmc_small,k = 3) + +# This plot shows the cells projected onto the top 2 principal +# components (PCs) of the topic model mixture proportions. +Idents(pbmc_small) <- pbmc_small$letter.idents +DimPlot(pbmc_small,reduction = "pca") + +# Once fitted topic model is extracted, many functions from the +# fastTopics package can be used. For example, the Structure plot +# provides an evocative visual summary of the estimated mixture +# proportions for each cell. +fit <- Misc(Reductions(pbmc_small,"multinom_topic_model")) +structure_plot(fit,grouping = Idents(pbmc_small),gap = 5) + +} +\references{ +Dey, K. K., Hsiao, C. J. and Stephens, M. (2017). Visualizing the +structure of RNA-seq expression data using grade of membership +models. \emph{PLoS Genetics} \bold{13}, e1006599. +} \seealso{ \code{\link[fastTopics]{fit_topic_model}} } +\author{ +Peter Carbonetto +} From 75e624048ee6b7d7629d8245e004f79e7dd2e234 Mon Sep 17 00:00:00 2001 From: Peter Carbonetto Date: Thu, 14 Jan 2021 14:07:30 -0600 Subject: [PATCH 04/50] Made several improvements to the roxygen2 docs for FitTopicModel, and fixed the handling of the features (in particular, we avoid using VariableFeatures). --- DESCRIPTION | 4 +-- NAMESPACE | 2 ++ R/fasttopics.R | 85 ++++++++++++++++++++++++++++++++++++-------- man/FitTopicModel.Rd | 70 ++++++++++++++++++++++++++++++------ 4 files changed, 134 insertions(+), 27 deletions(-) diff --git a/DESCRIPTION b/DESCRIPTION index 4e5b7a7..ea54492 100644 --- a/DESCRIPTION +++ b/DESCRIPTION @@ -1,7 +1,7 @@ Package: SeuratWrappers Title: Community-Provided Methods and Extensions for the Seurat Object -Version: 0.3.1 -Date: 2021-01-11 +Version: 0.3.2 +Date: 2021-01-14 Authors@R: c( person(given = 'Rahul', family = 'Satija', email = 'rsatija@nygenome.org', role = 'aut', comment = c(ORCID = '0000-0001-9448-8833')), person(given = 'Andrew', family = 'Butler', email = 'abutler@nygenome.org', role = 'aut', comment = c(ORCID = '0000-0003-3608-0463')), diff --git a/NAMESPACE b/NAMESPACE index 53101f0..2cb4f67 100644 --- a/NAMESPACE +++ b/NAMESPACE @@ -24,6 +24,7 @@ export(RunVelocity) export(as.cell_data_set) importFrom(BiocManager,install) importFrom(Matrix,Matrix) +importFrom(Matrix,colSums) importFrom(Matrix,t) importFrom(Matrix,writeMM) importFrom(Seurat,"DefaultAssay<-") @@ -56,6 +57,7 @@ importFrom(Seurat,as.Graph) importFrom(Seurat,as.Seurat) importFrom(Seurat,as.SingleCellExperiment) importFrom(cowplot,theme_cowplot) +importFrom(fastTopics,fit_topic_model) importFrom(ggplot2,aes_string) importFrom(ggplot2,geom_line) importFrom(ggplot2,geom_point) diff --git a/R/fasttopics.R b/R/fasttopics.R index b082ca2..f0a6086 100644 --- a/R/fasttopics.R +++ b/R/fasttopics.R @@ -1,6 +1,28 @@ #' @title Fit a Multinomial Topic Model Using fastTopics #' -#' @description Add description here. +#' @description Fits a multinomial topic model to the count data, +#' hiding most of the complexities of model fitting. The default +#' optimization settings used here are intended to work well in a wide +#' range of data sets, although some fine-tuning may be needed for +#' more difficult cases. +#' +#' @details The topic model can be understood as a dimensionality +#' reduction, in which the mixture proportions matrix, \code{L}, an n +#' x k matrix, defines a projection of the cells onto a +#' (k–1)-dimension space (n is the number of cells and k is the number +#' of topics). Therefore, we use the mixture proportions to define the +#' \dQuote{cell embedding} in the \code{DimReduc} object. +#' +#' There is a confusing bit of terminology here, because "loadings" +#' means something different depending on the context In fastTopics, +#' the \code{L} matrix is sometimes referred to as the "loadings +#' matrix" (this is the convention used in factor analysis), but it is +#' \emph{not} the same as the "feature loadings" matrix used here, +#' which adopts the convention used for principal component +#' analysis. To avoid confusion, we only refer to \code{L} as the +#' mixture proportions matrix. +#' +#' See \code{\link[fastTopics]{fit_topic_model}} for more details. #' #' @param object A Seurat object. #' @@ -10,8 +32,8 @@ #' assay of the object. #' #' @param features A list of features to use for fitting the model. If -#' \code{features = NULL}, all variable features; see -#' \code{\link[Seurat]{VariableFeatures}}. +#' \code{features = NULL}, \emph{all} features are used; see +#' \code{\link[Seurat]{VariableFeatures}}. NOTE: EXPAND ON THIS. #' #' @param reduction.name Name of the outputted reduction. #' @@ -22,15 +44,40 @@ #' \code{\link[fastTopics]{fit_poisson_nmf}} for an explanation of the #' output. #' -#' @param \dots \dots Additional arguments passed to -#' \code{fit_topic_model}; see -#' \code{\link[fastTopics]{fit_topic_model}} for details -#' -#' @return Describe the return value here. -#' -#' Clarify that, unfortunately, "factors" and "loadings" in fastTopics -#' mean the opposite of what they mean in Seurat. -#' +#' @param \dots Additional arguments passed to \code{fit_topic_model}; +#' see \code{\link[fastTopics]{fit_topic_model}} for details. +#' +#' @return A Seurat object with the multinomial topic model fit stored +#' as a \code{\link[Seurat]{DimReduc}} object. The cell embeddings +#' are the mixture proportions, stored as an n x k matrix in the +#' \code{cell.embeddings} slot, where n is the number of cells, and k +#' is the number of topics. This is the same as the \code{L} matrix +#' output in \code{fit_topic_model}. +#' +#' The feature loadings (the \code{feature.loadings} slot) are an m x +#' k matrix in which column i is the vector of relative gene +#' expression levels in topic i. This is the same as the \code{F} +#' output in \code{fit_topic_model}. +#' +#' Apply \code{\link[Seurat]{Misc}} to the \code{DimReduce} object to +#' access the \dQuote{"multinom_topic_model_fit"} object outputted by +#' \code{\link[fastTopics]{fit_topic_model}}, which contains more +#' information about the model fit; see the example for an +#' illustration of how to do this, and see +#' \code{\link[fastTopics]{fit_topic_model}} more information about +#' the "multinom_topic_model_fit" object. +#' +#' An additional PCA dimension reduction is provided to, for example, +#' quickly visualize the cells using +#' \code{\link[Seurat]{DimPlot}}. The principal components are +#' computed from the topic model mixture proportions. However, this is +#' for convenience only, and we recommend extracting the +#' \dQuote{"multinom_topic_model_fit"} object and dedicated +#' visualization tools such \code{\link[fastTopics]{structure_plot}} +#' that are provided in the fastTopics package. +#' +#' NOTE: There are only k - 1 PCs. +#' #' @author Peter Carbonetto #' #' @references @@ -41,13 +88,15 @@ #' @seealso \code{\link[fastTopics]{fit_topic_model}} #' #' @examples +#' library(Seurat) +#' library(fastTopics) #' set.seed(1) #' #' # Load the PBMC data. #' data(pbmc_small) #' #' # Fit the multinomial topic model to the raw UMI count data; no -#' # pre-processing is needed. +#' # pre-processing is needed. Note that all #' pbmc_small <- FitTopicModel(pbmc_small,k = 3) #' #' # This plot shows the cells projected onto the top 2 principal @@ -63,7 +112,9 @@ #' structure_plot(fit,grouping = Idents(pbmc_small),gap = 5) #' #' @importFrom stats prcomp +#' @importFrom Matrix colSums #' @importFrom Matrix t +#' @importFrom fastTopics fit_topic_model #' #' @export #' @@ -80,15 +131,19 @@ FitTopicModel <- function (object, k = 3, assay = NULL, features = NULL, # (cells) and m is the number of selected genes. assay <- assay %||% DefaultAssay(object) DefaultAssay(object) <- assay - features <- features %||% VariableFeatures(object) X <- GetAssayData(object,"counts") - if (length(features) == 0) + if (is.null(features)) features <- rownames(X) else features <- intersect(features,rownames(X)) X <- X[features,] X <- t(X) + # Remove all-zero columns. + i <- which(colSums(X > 0) >= 1) + features <- features[i] + X <- X[,i] + # Fit the multinomial topic model using fastTopics. fit <- fit_topic_model(X,k,verbose = verbose,...) class(fit) <- c("list","multinom_topic_model_fit") diff --git a/man/FitTopicModel.Rd b/man/FitTopicModel.Rd index c9606a8..7c59509 100644 --- a/man/FitTopicModel.Rd +++ b/man/FitTopicModel.Rd @@ -24,8 +24,8 @@ FitTopicModel( assay of the object.} \item{features}{A list of features to use for fitting the model. If -\code{features = NULL}, all variable features; see -\code{\link[Seurat]{VariableFeatures}}.} +\code{features = NULL}, \emph{all} features are used; see +\code{\link[Seurat]{VariableFeatures}}. NOTE: EXPAND ON THIS.} \item{reduction.name}{Name of the outputted reduction.} @@ -36,27 +36,77 @@ progress of the model fitting is printed to the console. See \code{\link[fastTopics]{fit_poisson_nmf}} for an explanation of the output.} -\item{\dots}{\dots Additional arguments passed to -\code{fit_topic_model}; see -\code{\link[fastTopics]{fit_topic_model}} for details} +\item{\dots}{Additional arguments passed to \code{fit_topic_model}; +see \code{\link[fastTopics]{fit_topic_model}} for details.} } \value{ -Describe the return value here. +A Seurat object with the multinomial topic model fit stored +as a \code{\link[Seurat]{DimReduc}} object. The cell embeddings +are the mixture proportions, stored as an n x k matrix in the +\code{cell.embeddings} slot, where n is the number of cells, and k +is the number of topics. This is the same as the \code{L} matrix +output in \code{fit_topic_model}. -Clarify that, unfortunately, "factors" and "loadings" in fastTopics -mean the opposite of what they mean in Seurat. +The feature loadings (the \code{feature.loadings} slot) are an m x +k matrix in which column i is the vector of relative gene +expression levels in topic i. This is the same as the \code{F} +output in \code{fit_topic_model}. + +Apply \code{\link[Seurat]{Misc}} to the \code{DimReduce} object to +access the \dQuote{"multinom_topic_model_fit"} object outputted by +\code{\link[fastTopics]{fit_topic_model}}, which contains more +information about the model fit; see the example for an +illustration of how to do this, and see +\code{\link[fastTopics]{fit_topic_model}} more information about +the "multinom_topic_model_fit" object. + +An additional PCA dimension reduction is provided to, for example, +quickly visualize the cells using +\code{\link[Seurat]{DimPlot}}. The principal components are +computed from the topic model mixture proportions. However, this is +for convenience only, and we recommend extracting the +\dQuote{"multinom_topic_model_fit"} object and dedicated +visualization tools such \code{\link[fastTopics]{structure_plot}} +that are provided in the fastTopics package. + +NOTE: There are only k - 1 PCs. } \description{ -Add description here. +Fits a multinomial topic model to the count data, + hiding most of the complexities of model fitting. The default + optimization settings used here are intended to work well in a wide + range of data sets, although some fine-tuning may be needed for + more difficult cases. +} +\details{ +The topic model can be understood as a dimensionality +reduction, in which the mixture proportions matrix, \code{L}, an n +x k matrix, defines a projection of the cells onto a +(k–1)-dimension space (n is the number of cells and k is the number +of topics). Therefore, we use the mixture proportions to define the +\dQuote{cell embedding} in the \code{DimReduc} object. + +There is a confusing bit of terminology here, because "loadings" +means something different depending on the context In fastTopics, +the \code{L} matrix is sometimes referred to as the "loadings +matrix" (this is the convention used in factor analysis), but it is +\emph{not} the same as the "feature loadings" matrix used here, +which adopts the convention used for principal component +analysis. To avoid confusion, we only refer to \code{L} as the +mixture proportions matrix. + +See \code{\link[fastTopics]{fit_topic_model}} for more details. } \examples{ +library(Seurat) +library(fastTopics) set.seed(1) # Load the PBMC data. data(pbmc_small) # Fit the multinomial topic model to the raw UMI count data; no -# pre-processing is needed. +# pre-processing is needed. Note that all pbmc_small <- FitTopicModel(pbmc_small,k = 3) # This plot shows the cells projected onto the top 2 principal From d362c19992f7434024d004b0857a05d50b7ad464 Mon Sep 17 00:00:00 2001 From: Peter Carbonetto Date: Fri, 15 Jan 2021 14:10:00 -0600 Subject: [PATCH 05/50] Improved fasttopics example; fixed PCA reduction key. --- R/fasttopics.R | 16 +++++++++------- man/FitTopicModel.Rd | 8 +++++--- 2 files changed, 14 insertions(+), 10 deletions(-) diff --git a/R/fasttopics.R b/R/fasttopics.R index f0a6086..911a84b 100644 --- a/R/fasttopics.R +++ b/R/fasttopics.R @@ -96,12 +96,14 @@ #' data(pbmc_small) #' #' # Fit the multinomial topic model to the raw UMI count data; no -#' # pre-processing is needed. Note that all +#' # pre-processing is needed. #' pbmc_small <- FitTopicModel(pbmc_small,k = 3) #' -#' # This plot shows the cells projected onto the top 2 principal +#' # This plot shows the cells projected onto the 2 principal #' # components (PCs) of the topic model mixture proportions. -#' Idents(pbmc_small) <- pbmc_small$letter.idents +#' DimPlot(pbmc_small,reduction = "pca_topics") +#' +#' # Compare against PCA performed on the transformed count data. #' DimPlot(pbmc_small,reduction = "pca") #' #' # Once fitted topic model is extracted, many functions from the @@ -162,11 +164,11 @@ FitTopicModel <- function (object, k = 3, assay = NULL, features = NULL, # Add a PCA dimension reduction calculated from the mixture # proportions. out <- prcomp(fit$L) - colnames(out$x) <- paste0("PC_",1:k) - colnames(out$rotation) <- paste0("PC_",1:k) - object[["pca"]] <- + colnames(out$x) <- paste0("TOPICPC_",1:k) + colnames(out$rotation) <- paste0("TOPICPC_",1:k) + object[["pca_topics"]] <- CreateDimReducObject(out$x[,-k],out$rotation[,-k],assay = assay, - key = "PC_",global = TRUE) + key = "TOPICPC_",global = TRUE) # Output the updated Seurat object. return(LogSeuratCommand(object)) diff --git a/man/FitTopicModel.Rd b/man/FitTopicModel.Rd index 7c59509..1793bfa 100644 --- a/man/FitTopicModel.Rd +++ b/man/FitTopicModel.Rd @@ -106,12 +106,14 @@ set.seed(1) data(pbmc_small) # Fit the multinomial topic model to the raw UMI count data; no -# pre-processing is needed. Note that all +# pre-processing is needed. pbmc_small <- FitTopicModel(pbmc_small,k = 3) -# This plot shows the cells projected onto the top 2 principal +# This plot shows the cells projected onto the 2 principal # components (PCs) of the topic model mixture proportions. -Idents(pbmc_small) <- pbmc_small$letter.idents +DimPlot(pbmc_small,reduction = "pca_topics") + +# Compare against PCA performed on the transformed count data. DimPlot(pbmc_small,reduction = "pca") # Once fitted topic model is extracted, many functions from the From 8a6b8aec5578aaa511303466935d52d5a4b81e03 Mon Sep 17 00:00:00 2001 From: Peter Carbonetto Date: Fri, 15 Jan 2021 14:30:56 -0600 Subject: [PATCH 06/50] Revised the FitTopicModel roxygen2 docs. --- R/fasttopics.R | 87 +++++++++++++++++++++++--------------------- man/FitTopicModel.Rd | 82 +++++++++++++++++++---------------------- 2 files changed, 83 insertions(+), 86 deletions(-) diff --git a/R/fasttopics.R b/R/fasttopics.R index 911a84b..feedf96 100644 --- a/R/fasttopics.R +++ b/R/fasttopics.R @@ -1,26 +1,29 @@ +# TO DO: +# - Implement FitPoissonNMF. +# - Implement vignette. + #' @title Fit a Multinomial Topic Model Using fastTopics #' -#' @description Fits a multinomial topic model to the count data, +#' @description Fits a multinomial topic model to the raw count data, #' hiding most of the complexities of model fitting. The default #' optimization settings used here are intended to work well in a wide #' range of data sets, although some fine-tuning may be needed for -#' more difficult cases. +#' larger or more complex data sets. #' #' @details The topic model can be understood as a dimensionality #' reduction, in which the mixture proportions matrix, \code{L}, an n #' x k matrix, defines a projection of the cells onto a #' (k–1)-dimension space (n is the number of cells and k is the number -#' of topics). Therefore, we use the mixture proportions to define the -#' \dQuote{cell embedding} in the \code{DimReduc} object. +#' of topics). The topic mixture proportions define the \dQuote{cell +#' embedding} in the \code{DimReduc} object. #' -#' There is a confusing bit of terminology here, because "loadings" -#' means something different depending on the context In fastTopics, +#' \emph{A warning about confusing terminology:} In fastTopics, #' the \code{L} matrix is sometimes referred to as the "loadings -#' matrix" (this is the convention used in factor analysis), but it is -#' \emph{not} the same as the "feature loadings" matrix used here, -#' which adopts the convention used for principal component -#' analysis. To avoid confusion, we only refer to \code{L} as the -#' mixture proportions matrix. +#' matrix" (because this is the convention used in factor +#' analysis). However, this is \emph{not} the same as the "feature +#' loadings" matrix used in Seurat, which adopts the convention used n +#' principal component analysis. To avoid confusion, we refer to +#' \code{L} as the mixture proportions matrix. #' #' See \code{\link[fastTopics]{fit_topic_model}} for more details. #' @@ -32,8 +35,9 @@ #' assay of the object. #' #' @param features A list of features to use for fitting the model. If -#' \code{features = NULL}, \emph{all} features are used; see -#' \code{\link[Seurat]{VariableFeatures}}. NOTE: EXPAND ON THIS. +#' \code{features = NULL}, \emph{all} features are used; in +#' particular, ; see \code{\link[Seurat]{VariableFeatures}} is \emph{not +#' used to pre-select features. #' #' @param reduction.name Name of the outputted reduction. #' @@ -47,36 +51,34 @@ #' @param \dots Additional arguments passed to \code{fit_topic_model}; #' see \code{\link[fastTopics]{fit_topic_model}} for details. #' -#' @return A Seurat object with the multinomial topic model fit stored -#' as a \code{\link[Seurat]{DimReduc}} object. The cell embeddings -#' are the mixture proportions, stored as an n x k matrix in the -#' \code{cell.embeddings} slot, where n is the number of cells, and k -#' is the number of topics. This is the same as the \code{L} matrix -#' output in \code{fit_topic_model}. -#' -#' The feature loadings (the \code{feature.loadings} slot) are an m x -#' k matrix in which column i is the vector of relative gene -#' expression levels in topic i. This is the same as the \code{F} -#' output in \code{fit_topic_model}. +#' @return A Seurat object, in which the multinomial topic model fit +#' is stored as a Seurat \code{\link[Seurat]{DimReduc}} object. The +#' cell embeddings (stored in the \code{cell.embeddings} slot) are the +#' mixture proportions; this is the n x k matrix \code{L} outputted by +#' \code{fit_topic_model} (n is the number of cells and k is the +#' number of topics). +#' +#' The feature loadings (stored in the \code{feature.loadings} slot) +#' are an m x k matrix of relative expression levels; this is the +#' matrix \code{F} outputted by \code{fit_topic_model}. #' #' Apply \code{\link[Seurat]{Misc}} to the \code{DimReduce} object to #' access the \dQuote{"multinom_topic_model_fit"} object outputted by #' \code{\link[fastTopics]{fit_topic_model}}, which contains more -#' information about the model fit; see the example for an -#' illustration of how to do this, and see +#' information about the multinomial topic model fit; see the example +#' here for an illustration of how to access the model fit, and see #' \code{\link[fastTopics]{fit_topic_model}} more information about #' the "multinom_topic_model_fit" object. #' -#' An additional PCA dimension reduction is provided to, for example, -#' quickly visualize the cells using -#' \code{\link[Seurat]{DimPlot}}. The principal components are -#' computed from the topic model mixture proportions. However, this is -#' for convenience only, and we recommend extracting the -#' \dQuote{"multinom_topic_model_fit"} object and dedicated -#' visualization tools such \code{\link[fastTopics]{structure_plot}} -#' that are provided in the fastTopics package. -#' -#' NOTE: There are only k - 1 PCs. +#' An additional PCA dimension reduction on \code{k-1} dimensions is +#' provided. This could be useful, for example, to quickly visualize +#' the cells using \code{\link[Seurat]{DimPlot}}. The principal +#' components are computed from the topic mixture +#' proportions. However, this reduction is provided for convenience +#' only, and we recommend to extract the +#' \dQuote{"multinom_topic_model_fit"} object and use the dedicated +#' visualization tools that are provided in the fastTopics package +#' such as \code{\link[fastTopics]{structure_plot}}. #' #' @author Peter Carbonetto #' @@ -96,20 +98,21 @@ #' data(pbmc_small) #' #' # Fit the multinomial topic model to the raw UMI count data; no -#' # pre-processing is needed. +#' # pre-processing or pre-selection of genes is needed. #' pbmc_small <- FitTopicModel(pbmc_small,k = 3) #' #' # This plot shows the cells projected onto the 2 principal -#' # components (PCs) of the topic model mixture proportions. +#' # components (PCs) of the topic mixture proportions. #' DimPlot(pbmc_small,reduction = "pca_topics") #' -#' # Compare against PCA performed on the transformed count data. +#' # Compare this against the top two PCs of the transformed count +#' # data. #' DimPlot(pbmc_small,reduction = "pca") #' #' # Once fitted topic model is extracted, many functions from the -#' # fastTopics package can be used. For example, the Structure plot -#' # provides an evocative visual summary of the estimated mixture -#' # proportions for each cell. +#' # fastTopics package can be used for analysis and visualization. For +#' # example, the Structure plot provides an evocative visual summary of +#' # the estimated mixture proportions for each cell. #' fit <- Misc(Reductions(pbmc_small,"multinom_topic_model")) #' structure_plot(fit,grouping = Idents(pbmc_small),gap = 5) #' diff --git a/man/FitTopicModel.Rd b/man/FitTopicModel.Rd index 1793bfa..1b6306b 100644 --- a/man/FitTopicModel.Rd +++ b/man/FitTopicModel.Rd @@ -23,10 +23,6 @@ FitTopicModel( \item{assay}{Name of assay to use; defaults to the default assay of the object.} -\item{features}{A list of features to use for fitting the model. If -\code{features = NULL}, \emph{all} features are used; see -\code{\link[Seurat]{VariableFeatures}}. NOTE: EXPAND ON THIS.} - \item{reduction.name}{Name of the outputted reduction.} \item{reduction.key}{Key for the outputted reduction.} @@ -40,60 +36,57 @@ output.} see \code{\link[fastTopics]{fit_topic_model}} for details.} } \value{ -A Seurat object with the multinomial topic model fit stored -as a \code{\link[Seurat]{DimReduc}} object. The cell embeddings -are the mixture proportions, stored as an n x k matrix in the -\code{cell.embeddings} slot, where n is the number of cells, and k -is the number of topics. This is the same as the \code{L} matrix -output in \code{fit_topic_model}. - -The feature loadings (the \code{feature.loadings} slot) are an m x -k matrix in which column i is the vector of relative gene -expression levels in topic i. This is the same as the \code{F} -output in \code{fit_topic_model}. +A Seurat object, in which the multinomial topic model fit +is stored as a Seurat \code{\link[Seurat]{DimReduc}} object. The +cell embeddings (stored in the \code{cell.embeddings} slot) are the +mixture proportions; this is the n x k matrix \code{L} outputted by +\code{fit_topic_model} (n is the number of cells and k is the +number of topics). + +The feature loadings (stored in the \code{feature.loadings} slot) +are an m x k matrix of relative expression levels; this is the +matrix \code{F} outputted by \code{fit_topic_model}. Apply \code{\link[Seurat]{Misc}} to the \code{DimReduce} object to access the \dQuote{"multinom_topic_model_fit"} object outputted by \code{\link[fastTopics]{fit_topic_model}}, which contains more -information about the model fit; see the example for an -illustration of how to do this, and see +information about the multinomial topic model fit; see the example +here for an illustration of how to access the model fit, and see \code{\link[fastTopics]{fit_topic_model}} more information about the "multinom_topic_model_fit" object. -An additional PCA dimension reduction is provided to, for example, -quickly visualize the cells using -\code{\link[Seurat]{DimPlot}}. The principal components are -computed from the topic model mixture proportions. However, this is -for convenience only, and we recommend extracting the -\dQuote{"multinom_topic_model_fit"} object and dedicated -visualization tools such \code{\link[fastTopics]{structure_plot}} -that are provided in the fastTopics package. - -NOTE: There are only k - 1 PCs. +An additional PCA dimension reduction on \code{k-1} dimensions is +provided. This could be useful, for example, to quickly visualize +the cells using \code{\link[Seurat]{DimPlot}}. The principal +components are computed from the topic mixture +proportions. However, this reduction is provided for convenience +only, and we recommend to extract the +\dQuote{"multinom_topic_model_fit"} object and use the dedicated +visualization tools that are provided in the fastTopics package +such as \code{\link[fastTopics]{structure_plot}}. } \description{ -Fits a multinomial topic model to the count data, +Fits a multinomial topic model to the raw count data, hiding most of the complexities of model fitting. The default optimization settings used here are intended to work well in a wide range of data sets, although some fine-tuning may be needed for - more difficult cases. + larger or more complex data sets. } \details{ The topic model can be understood as a dimensionality reduction, in which the mixture proportions matrix, \code{L}, an n x k matrix, defines a projection of the cells onto a (k–1)-dimension space (n is the number of cells and k is the number -of topics). Therefore, we use the mixture proportions to define the -\dQuote{cell embedding} in the \code{DimReduc} object. +of topics). The topic mixture proportions define the \dQuote{cell +embedding} in the \code{DimReduc} object. -There is a confusing bit of terminology here, because "loadings" -means something different depending on the context In fastTopics, +\emph{A warning about confusing terminology:} In fastTopics, the \code{L} matrix is sometimes referred to as the "loadings -matrix" (this is the convention used in factor analysis), but it is -\emph{not} the same as the "feature loadings" matrix used here, -which adopts the convention used for principal component -analysis. To avoid confusion, we only refer to \code{L} as the -mixture proportions matrix. +matrix" (because this is the convention used in factor +analysis). However, this is \emph{not} the same as the "feature +loadings" matrix used in Seurat, which adopts the convention used n +principal component analysis. To avoid confusion, we refer to +\code{L} as the mixture proportions matrix. See \code{\link[fastTopics]{fit_topic_model}} for more details. } @@ -106,20 +99,21 @@ set.seed(1) data(pbmc_small) # Fit the multinomial topic model to the raw UMI count data; no -# pre-processing is needed. +# pre-processing or pre-selection of genes is needed. pbmc_small <- FitTopicModel(pbmc_small,k = 3) # This plot shows the cells projected onto the 2 principal -# components (PCs) of the topic model mixture proportions. +# components (PCs) of the topic mixture proportions. DimPlot(pbmc_small,reduction = "pca_topics") -# Compare against PCA performed on the transformed count data. +# Compare this against the top two PCs of the transformed count +# data. DimPlot(pbmc_small,reduction = "pca") # Once fitted topic model is extracted, many functions from the -# fastTopics package can be used. For example, the Structure plot -# provides an evocative visual summary of the estimated mixture -# proportions for each cell. +# fastTopics package can be used for analysis and visualization. For +# example, the Structure plot provides an evocative visual summary of +# the estimated mixture proportions for each cell. fit <- Misc(Reductions(pbmc_small,"multinom_topic_model")) structure_plot(fit,grouping = Idents(pbmc_small),gap = 5) From cd3f14338de9a21af80c998a87c3cd202d323c7d Mon Sep 17 00:00:00 2001 From: Peter Carbonetto Date: Fri, 15 Jan 2021 14:51:45 -0600 Subject: [PATCH 07/50] Completed fast_topics_demo.R script. --- R/fasttopics.R | 6 +++++- docs/fast_topics_demo.R | 33 +++++++++++++++++++++------------ docs/fasttopics.Rmd | 11 +++++++++++ man/FitTopicModel.Rd | 3 ++- 4 files changed, 39 insertions(+), 14 deletions(-) diff --git a/R/fasttopics.R b/R/fasttopics.R index feedf96..fb09480 100644 --- a/R/fasttopics.R +++ b/R/fasttopics.R @@ -1,6 +1,9 @@ # TO DO: +# # - Implement FitPoissonNMF. +# # - Implement vignette. +# #' @title Fit a Multinomial Topic Model Using fastTopics #' @@ -87,7 +90,8 @@ #' structure of RNA-seq expression data using grade of membership #' models. \emph{PLoS Genetics} \bold{13}, e1006599. #' -#' @seealso \code{\link[fastTopics]{fit_topic_model}} +#' @seealso \code{\link{FitPoissonNMF}}, +#' \code{\link[fastTopics]{fit_topic_model}} #' #' @examples #' library(Seurat) diff --git a/docs/fast_topics_demo.R b/docs/fast_topics_demo.R index b233014..acbe915 100644 --- a/docs/fast_topics_demo.R +++ b/docs/fast_topics_demo.R @@ -1,30 +1,39 @@ # Load the packages used in the analysis below. library(Seurat) library(SeuratData) -library(SeuratWrappers) +# library(SeuratWrappers) library(fastTopics) # Set seed to generate results that are reproducible. set.seed(1) -# Load the UMI count data. +# Load (and, if necessary, install) the PBMC 3k data. InstallData("pbmc3k") data(pbmc3k) -# No pre-processing is needed. +# No pre-processing or pre-selection of genes is needed. -# Fit topic model to UMI counts. Note that it may take several minutes -# for the model fitting to complete. +# Fit the multinomial topic model to raw UMI counts. Note that it may +# take several minutes for the model fitting to complete on this data +# set. pbmc3k <- FitTopicModel(pbmc3k,k = 6) -# Plot the top two PCs of the mixture proportions. +# This plot shows the cells projected onto the two principal +# components (PCs) of the topic mixture proportions. Idents(pbmc3k) <- pbmc3k$seurat_annotations -DimPlot(pbmc3k) - -# Create a Structure plot. +DimPlot(pbmc3k,reduction = "pca_topics",pt.size = 1) + +# Compare this against the top two PCs of the transformed counts. +pbmc3k <- FindVariableFeatures(pbmc3k) +pbmc3k <- ScaleData(pbmc3k) +pbmc3k <- RunPCA(pbmc3k) +DimPlot(pbmc3k,reduction = "pca",pt.size = 1) + +# Once fitted topic model is extracted, many functions from the +# fastTopics package can be used for analysis and visualization. For +# example, the Structure plot provides an evocative visual summary of +# the estimated mixture proportions for each cell. fit <- Misc(Reductions(pbmc3k,"multinom_topic_model")) -p <- structure_plot(fit,grouping = Idents(pbmc3k),gap = 25) +structure_plot(fit,grouping = Idents(pbmc3k),gap = 25) -# Fit non-negative matrix factorization to UMI counts. -# TO DO. diff --git a/docs/fasttopics.Rmd b/docs/fasttopics.Rmd index e69de29..07dacd8 100644 --- a/docs/fasttopics.Rmd +++ b/docs/fasttopics.Rmd @@ -0,0 +1,11 @@ +--- +title: "Running GLM-PCA on a Seurat Object" +date: 'Compiled: `r format(Sys.Date(), "%B %d, %Y")`' +output: + github_document: + html_preview: true + toc: true + toc_depth: 3 + html_document: + df_print: kable +--- diff --git a/man/FitTopicModel.Rd b/man/FitTopicModel.Rd index 1b6306b..3b78e1c 100644 --- a/man/FitTopicModel.Rd +++ b/man/FitTopicModel.Rd @@ -124,7 +124,8 @@ structure of RNA-seq expression data using grade of membership models. \emph{PLoS Genetics} \bold{13}, e1006599. } \seealso{ -\code{\link[fastTopics]{fit_topic_model}} +\code{\link{FitPoissonNMF}}, + \code{\link[fastTopics]{fit_topic_model}} } \author{ Peter Carbonetto From 811d618481fc926ff939bd12c89a5a30ccc13286 Mon Sep 17 00:00:00 2001 From: Peter Carbonetto Date: Fri, 15 Jan 2021 15:04:13 -0600 Subject: [PATCH 08/50] First build of the fasttopics vignette. --- docs/fast_topics_demo.R | 2 +- docs/fasttopics.Rmd | 41 +++- docs/fasttopics.html | 491 ++++++++++++++++++++++++++++++++++++++++ docs/fasttopics.md | 102 +++++++++ 4 files changed, 632 insertions(+), 4 deletions(-) create mode 100644 docs/fasttopics.html create mode 100644 docs/fasttopics.md diff --git a/docs/fast_topics_demo.R b/docs/fast_topics_demo.R index acbe915..87bb9b1 100644 --- a/docs/fast_topics_demo.R +++ b/docs/fast_topics_demo.R @@ -1,7 +1,7 @@ # Load the packages used in the analysis below. library(Seurat) library(SeuratData) -# library(SeuratWrappers) +library(SeuratWrappers) library(fastTopics) # Set seed to generate results that are reproducible. diff --git a/docs/fasttopics.Rmd b/docs/fasttopics.Rmd index 07dacd8..da3fdb6 100644 --- a/docs/fasttopics.Rmd +++ b/docs/fasttopics.Rmd @@ -1,11 +1,46 @@ --- -title: "Running GLM-PCA on a Seurat Object" +title: "Add title here" date: 'Compiled: `r format(Sys.Date(), "%B %d, %Y")`' output: github_document: html_preview: true - toc: true - toc_depth: 3 html_document: df_print: kable --- + +*Add introductory text here.* + +*Add citation here.* + +We begin by loading the packages used to perform the analysis. + +```{r load-pkgs, message=FALSE, warning=FALSE} + +``` + +```{r load-packages} +library(Seurat) +library(SeuratData) +library(SeuratWrappers) +library(fastTopics) +``` + +We set the seed so that the results can be reproduced. + +```{r set-seed} +set.seed(1) +``` + +Load---and, if necessary, install---the PBMC 3k data set. + +```{r load-data} +InstallData("pbmc3k") +data(pbmc3k) +``` + +This is the version of R and the packages that were used to generate +these results. + +```{r session-info} +sessionInfo() +``` diff --git a/docs/fasttopics.html b/docs/fasttopics.html new file mode 100644 index 0000000..bf898df --- /dev/null +++ b/docs/fasttopics.html @@ -0,0 +1,491 @@ + + + + + + + + + + + + + +Add title here + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + +
+ + + + + + + +

Add introductory text here.

+

Add citation here.

+

We begin by loading the packages used to perform the analysis.

+
library(Seurat)
+library(SeuratData)
+library(SeuratWrappers)
+library(fastTopics)
+

We set the seed so that the results can be reproduced.

+
set.seed(1)
+

Load—and, if necessary, install—the PBMC 3k data set.

+
InstallData("pbmc3k")
+
## Warning: The following packages are already installed and will not be
+## reinstalled: pbmc3k
+
data(pbmc3k)
+

This is the version of R and the packages that were used to generate these results.

+
sessionInfo()
+
## R version 3.6.2 (2019-12-12)
+## Platform: x86_64-apple-darwin15.6.0 (64-bit)
+## Running under: macOS Catalina 10.15.7
+## 
+## Matrix products: default
+## BLAS:   /Library/Frameworks/R.framework/Versions/3.6/Resources/lib/libRblas.0.dylib
+## LAPACK: /Library/Frameworks/R.framework/Versions/3.6/Resources/lib/libRlapack.dylib
+## 
+## locale:
+## [1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8
+## 
+## attached base packages:
+## [1] stats     graphics  grDevices utils     datasets  methods   base     
+## 
+## other attached packages:
+## [1] fastTopics_0.4-23       SeuratWrappers_0.3.2    pbmc3k.SeuratData_3.1.4
+## [4] SeuratData_0.2.1        Seurat_3.2.3           
+## 
+## loaded via a namespace (and not attached):
+##   [1] Rtsne_0.15            colorspace_1.4-1      deldir_0.1-29        
+##   [4] ggridges_0.5.2        spatstat.data_1.4-3   leiden_0.3.3         
+##   [7] listenv_0.8.0         MatrixModels_0.4-1    remotes_2.1.0        
+##  [10] ggrepel_0.9.0         fansi_0.4.0           codetools_0.2-16     
+##  [13] splines_3.6.2         knitr_1.26            polyclip_1.10-0      
+##  [16] zeallot_0.1.0         jsonlite_1.6          mcmc_0.9-6           
+##  [19] ica_1.0-2             cluster_2.1.0         png_0.1-7            
+##  [22] uwot_0.1.10           shiny_1.4.0           sctransform_0.3.2    
+##  [25] BiocManager_1.30.10   compiler_3.6.2        httr_1.4.2           
+##  [28] backports_1.1.5       assertthat_0.2.1      Matrix_1.2-18        
+##  [31] fastmap_1.0.1         lazyeval_0.2.2        cli_2.0.0            
+##  [34] later_1.0.0           htmltools_0.4.0       quantreg_5.54        
+##  [37] prettyunits_1.1.1     tools_3.6.2           rsvd_1.0.2           
+##  [40] igraph_1.2.5          coda_0.19-3           gtable_0.3.0         
+##  [43] glue_1.3.1            RANN_2.6.1            reshape2_1.4.3       
+##  [46] dplyr_0.8.3           rappdirs_0.3.1        Rcpp_1.0.5           
+##  [49] spatstat_1.64-1       scattermore_0.7       vctrs_0.2.1          
+##  [52] nlme_3.1-142          lmtest_0.9-38         xfun_0.11            
+##  [55] stringr_1.4.0         globals_0.13.0        mime_0.8             
+##  [58] miniUI_0.1.1.1        lifecycle_0.1.0       irlba_2.3.3          
+##  [61] goftest_1.2-2         future_1.18.0         MASS_7.3-51.4        
+##  [64] zoo_1.8-7             scales_1.1.0          hms_0.5.2            
+##  [67] promises_1.1.0        spatstat.utils_1.17-0 parallel_3.6.2       
+##  [70] SparseM_1.78          RColorBrewer_1.1-2    yaml_2.2.0           
+##  [73] reticulate_1.16       pbapply_1.4-3         gridExtra_2.3        
+##  [76] ggplot2_3.3.0         rpart_4.1-15          stringi_1.4.3        
+##  [79] rlang_0.4.5           pkgconfig_2.0.3       matrixStats_0.56.0   
+##  [82] evaluate_0.14         lattice_0.20-38       ROCR_1.0-11          
+##  [85] purrr_0.3.3           tensor_1.5            patchwork_1.0.1      
+##  [88] htmlwidgets_1.5.1     cowplot_1.0.0         tidyselect_0.2.5     
+##  [91] RcppAnnoy_0.0.18      plyr_1.8.5            magrittr_1.5         
+##  [94] R6_2.4.1              pillar_1.4.3          mgcv_1.8-31          
+##  [97] fitdistrplus_1.1-1    survival_3.1-8        abind_1.4-5          
+## [100] tibble_2.1.3          future.apply_1.6.0    crayon_1.3.4         
+## [103] KernSmooth_2.23-16    plotly_4.9.2          rmarkdown_2.3        
+## [106] progress_1.2.2        grid_3.6.2            data.table_1.12.8    
+## [109] digest_0.6.23         xtable_1.8-4          tidyr_1.0.0          
+## [112] httpuv_1.5.2          MCMCpack_1.4-5        RcppParallel_4.4.2   
+## [115] munsell_0.5.0         viridisLite_0.3.0     quadprog_1.5-8
+ + + + +
+ + + + + + + + + + + + + + + diff --git a/docs/fasttopics.md b/docs/fasttopics.md new file mode 100644 index 0000000..a93f4a0 --- /dev/null +++ b/docs/fasttopics.md @@ -0,0 +1,102 @@ +Add title here +================ +Compiled: January 15, 2021 + +*Add introductory text here.* + +*Add citation here.* + +We begin by loading the packages used to perform the analysis. + +``` r +library(Seurat) +library(SeuratData) +library(SeuratWrappers) +library(fastTopics) +``` + +We set the seed so that the results can be reproduced. + +``` r +set.seed(1) +``` + +Load—and, if necessary, install—the PBMC 3k data + set. + +``` r +InstallData("pbmc3k") +``` + + ## Warning: The following packages are already installed and will not be + ## reinstalled: pbmc3k + +``` r +data(pbmc3k) +``` + +This is the version of R and the packages that were used to generate +these results. + +``` r +sessionInfo() +``` + + ## R version 3.6.2 (2019-12-12) + ## Platform: x86_64-apple-darwin15.6.0 (64-bit) + ## Running under: macOS Catalina 10.15.7 + ## + ## Matrix products: default + ## BLAS: /Library/Frameworks/R.framework/Versions/3.6/Resources/lib/libRblas.0.dylib + ## LAPACK: /Library/Frameworks/R.framework/Versions/3.6/Resources/lib/libRlapack.dylib + ## + ## locale: + ## [1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8 + ## + ## attached base packages: + ## [1] stats graphics grDevices utils datasets methods base + ## + ## other attached packages: + ## [1] fastTopics_0.4-23 SeuratWrappers_0.3.2 pbmc3k.SeuratData_3.1.4 + ## [4] SeuratData_0.2.1 Seurat_3.2.3 + ## + ## loaded via a namespace (and not attached): + ## [1] Rtsne_0.15 colorspace_1.4-1 deldir_0.1-29 + ## [4] ggridges_0.5.2 spatstat.data_1.4-3 leiden_0.3.3 + ## [7] listenv_0.8.0 MatrixModels_0.4-1 remotes_2.1.0 + ## [10] ggrepel_0.9.0 fansi_0.4.0 codetools_0.2-16 + ## [13] splines_3.6.2 knitr_1.26 polyclip_1.10-0 + ## [16] zeallot_0.1.0 jsonlite_1.6 mcmc_0.9-6 + ## [19] ica_1.0-2 cluster_2.1.0 png_0.1-7 + ## [22] uwot_0.1.10 shiny_1.4.0 sctransform_0.3.2 + ## [25] BiocManager_1.30.10 compiler_3.6.2 httr_1.4.2 + ## [28] backports_1.1.5 assertthat_0.2.1 Matrix_1.2-18 + ## [31] fastmap_1.0.1 lazyeval_0.2.2 cli_2.0.0 + ## [34] later_1.0.0 htmltools_0.4.0 quantreg_5.54 + ## [37] prettyunits_1.1.1 tools_3.6.2 rsvd_1.0.2 + ## [40] igraph_1.2.5 coda_0.19-3 gtable_0.3.0 + ## [43] glue_1.3.1 RANN_2.6.1 reshape2_1.4.3 + ## [46] dplyr_0.8.3 rappdirs_0.3.1 Rcpp_1.0.5 + ## [49] spatstat_1.64-1 scattermore_0.7 vctrs_0.2.1 + ## [52] nlme_3.1-142 lmtest_0.9-38 xfun_0.11 + ## [55] stringr_1.4.0 globals_0.13.0 mime_0.8 + ## [58] miniUI_0.1.1.1 lifecycle_0.1.0 irlba_2.3.3 + ## [61] goftest_1.2-2 future_1.18.0 MASS_7.3-51.4 + ## [64] zoo_1.8-7 scales_1.1.0 hms_0.5.2 + ## [67] promises_1.1.0 spatstat.utils_1.17-0 parallel_3.6.2 + ## [70] SparseM_1.78 RColorBrewer_1.1-2 yaml_2.2.0 + ## [73] reticulate_1.16 pbapply_1.4-3 gridExtra_2.3 + ## [76] ggplot2_3.3.0 rpart_4.1-15 stringi_1.4.3 + ## [79] rlang_0.4.5 pkgconfig_2.0.3 matrixStats_0.56.0 + ## [82] evaluate_0.14 lattice_0.20-38 ROCR_1.0-11 + ## [85] purrr_0.3.3 tensor_1.5 patchwork_1.0.1 + ## [88] htmlwidgets_1.5.1 cowplot_1.0.0 tidyselect_0.2.5 + ## [91] RcppAnnoy_0.0.18 plyr_1.8.5 magrittr_1.5 + ## [94] R6_2.4.1 pillar_1.4.3 mgcv_1.8-31 + ## [97] fitdistrplus_1.1-1 survival_3.1-8 abind_1.4-5 + ## [100] tibble_2.1.3 future.apply_1.6.0 crayon_1.3.4 + ## [103] KernSmooth_2.23-16 plotly_4.9.2 rmarkdown_2.3 + ## [106] progress_1.2.2 grid_3.6.2 data.table_1.12.8 + ## [109] digest_0.6.23 xtable_1.8-4 tidyr_1.0.0 + ## [112] httpuv_1.5.2 MCMCpack_1.4-5 RcppParallel_4.4.2 + ## [115] munsell_0.5.0 viridisLite_0.3.0 quadprog_1.5-8 From 8dee4810618d3870a965083bdadd7e71bfc02991 Mon Sep 17 00:00:00 2001 From: Peter Carbonetto Date: Fri, 15 Jan 2021 15:19:40 -0600 Subject: [PATCH 09/50] More additions to the fasttopics vignette. --- docs/fasttopics.Rmd | 61 +++++++++++++++++++++++++++++++++++++++------ 1 file changed, 54 insertions(+), 7 deletions(-) diff --git a/docs/fasttopics.Rmd b/docs/fasttopics.Rmd index da3fdb6..6b30d28 100644 --- a/docs/fasttopics.Rmd +++ b/docs/fasttopics.Rmd @@ -1,24 +1,29 @@ --- -title: "Add title here" +title: "Analyzing Seurat data using fastTopics" date: 'Compiled: `r format(Sys.Date(), "%B %d, %Y")`' output: github_document: html_preview: true html_document: df_print: kable + highlight: textmate + theme: readable --- *Add introductory text here.* +*Point to the fastTopics website and vignettes.* + *Add citation here.* +```{r knitr-opts, include=FALSE} +knitr::opts_chunk$set(comment = "#",collapse = TRUE,results = "hold", + fig.align = "center",dpi = 120) +``` + We begin by loading the packages used to perform the analysis. ```{r load-pkgs, message=FALSE, warning=FALSE} - -``` - -```{r load-packages} library(Seurat) library(SeuratData) library(SeuratWrappers) @@ -31,11 +36,53 @@ We set the seed so that the results can be reproduced. set.seed(1) ``` -Load---and, if necessary, install---the PBMC 3k data set. +Load---and, if necessary, install---the PBMC 3k data set containing +transcription profiles for 2,700 cells. -```{r load-data} +```{r load-data, warning=FALSE} InstallData("pbmc3k") data(pbmc3k) +dim(GetAssayData(pbmc3k)) +``` + +Fit the multinomial topic model to the raw UMI counts---*no +pre-processing or pre-selection of genes is needed.* Note that it may +take several minutes to complete this model fitting step. + +```{r fit-model, results="hide", cache=TRUE} +pbmc3k <- FitTopicModel(pbmc3k,k = 6) +``` + +To fit a topic model, we must specify $K$, the number of topics. +Here, we have chosen $K = 6$ topics. In most settings, a good choice +of $K$ will not be known in advance, so you will you want to explore +the results from topic models at different settings of $K$. + +This plot shows the cells projected onto the top two principal +components (PCs) obtained from the topic mixture proportions. + +```{r pca-1, fig.width=4, fig.height=3} +Idents(pbmc3k) <- pbmc3k$seurat_annotations +DimPlot(pbmc3k,reduction = "pca_topics",pt.size = 1) +``` + +Compare this against PCs 1 and 2 of the transformed counts: + +```{r pca-2, fig.width=4, fig.height=3} +pbmc3k <- FindVariableFeatures(pbmc3k) +pbmc3k <- ScaleData(pbmc3k) +pbmc3k <- RunPCA(pbmc3k) +DimPlot(pbmc3k,reduction = "pca",pt.size = 1) +``` + +Once fitted topic model is extracted, many functions from the +fastTopics package can be used for analysis and visualization. For +example, the Structure plot provides an evocative visual summary of +the estimated mixture proportions for each cell. + +```{r structure-plot, fig.width=7.5, fig.height=2} +fit <- Misc(Reductions(pbmc3k,"multinom_topic_model")) +structure_plot(fit,grouping = Idents(pbmc3k),gap = 25) ``` This is the version of R and the packages that were used to generate From da9b1c92aeb37a7817d62d485f066a23f2a1b7db Mon Sep 17 00:00:00 2001 From: Peter Carbonetto Date: Fri, 15 Jan 2021 16:01:08 -0600 Subject: [PATCH 10/50] Some minor adjustments to the fasttopics vignette. --- docs/fast_topics_demo.R | 39 --------------------------------------- docs/fasttopics.Rmd | 34 +++++++++++++++++++++++++--------- 2 files changed, 25 insertions(+), 48 deletions(-) delete mode 100644 docs/fast_topics_demo.R diff --git a/docs/fast_topics_demo.R b/docs/fast_topics_demo.R deleted file mode 100644 index 87bb9b1..0000000 --- a/docs/fast_topics_demo.R +++ /dev/null @@ -1,39 +0,0 @@ -# Load the packages used in the analysis below. -library(Seurat) -library(SeuratData) -library(SeuratWrappers) -library(fastTopics) - -# Set seed to generate results that are reproducible. -set.seed(1) - -# Load (and, if necessary, install) the PBMC 3k data. -InstallData("pbmc3k") -data(pbmc3k) - -# No pre-processing or pre-selection of genes is needed. - -# Fit the multinomial topic model to raw UMI counts. Note that it may -# take several minutes for the model fitting to complete on this data -# set. -pbmc3k <- FitTopicModel(pbmc3k,k = 6) - -# This plot shows the cells projected onto the two principal -# components (PCs) of the topic mixture proportions. -Idents(pbmc3k) <- pbmc3k$seurat_annotations -DimPlot(pbmc3k,reduction = "pca_topics",pt.size = 1) - -# Compare this against the top two PCs of the transformed counts. -pbmc3k <- FindVariableFeatures(pbmc3k) -pbmc3k <- ScaleData(pbmc3k) -pbmc3k <- RunPCA(pbmc3k) -DimPlot(pbmc3k,reduction = "pca",pt.size = 1) - -# Once fitted topic model is extracted, many functions from the -# fastTopics package can be used for analysis and visualization. For -# example, the Structure plot provides an evocative visual summary of -# the estimated mixture proportions for each cell. -fit <- Misc(Reductions(pbmc3k,"multinom_topic_model")) -structure_plot(fit,grouping = Idents(pbmc3k),gap = 25) - - diff --git a/docs/fasttopics.Rmd b/docs/fasttopics.Rmd index 6b30d28..404e5c1 100644 --- a/docs/fasttopics.Rmd +++ b/docs/fasttopics.Rmd @@ -14,7 +14,17 @@ output: *Point to the fastTopics website and vignettes.* -*Add citation here.* +If you find the **fastTopics** package useful for your work, please +cite: + +> Kushal K. Dey, Chiaowen Joyce Hsiao and Matthew Stephens (2017). +> [Visualizing the structure of RNA-seq expression data using grade of membership models.][countclust-paper] +> *PLoS Genetics* **13**, e1006599. +> +> Peter Carbonetto, Kevin Luo, Kushal Dey, Joyce Hsiao and Matthew +> Stephens (2021). fastTopics: fast algorithms for fitting topic models +> and non-negative matrix factorizations to count data. R package +> version 0.4-11. [https://github.com/stephenslab/fastTopics][fasttopics] ```{r knitr-opts, include=FALSE} knitr::opts_chunk$set(comment = "#",collapse = TRUE,results = "hold", @@ -28,6 +38,7 @@ library(Seurat) library(SeuratData) library(SeuratWrappers) library(fastTopics) +library(cowplot) ``` We set the seed so that the results can be reproduced. @@ -49,7 +60,7 @@ Fit the multinomial topic model to the raw UMI counts---*no pre-processing or pre-selection of genes is needed.* Note that it may take several minutes to complete this model fitting step. -```{r fit-model, results="hide", cache=TRUE} +```{r fit-model, results="hide", message=FALSE, cache=TRUE} pbmc3k <- FitTopicModel(pbmc3k,k = 6) ``` @@ -61,26 +72,28 @@ the results from topic models at different settings of $K$. This plot shows the cells projected onto the top two principal components (PCs) obtained from the topic mixture proportions. -```{r pca-1, fig.width=4, fig.height=3} +```{r pca-1, fig.width=4.5, fig.height=3} Idents(pbmc3k) <- pbmc3k$seurat_annotations -DimPlot(pbmc3k,reduction = "pca_topics",pt.size = 1) +DimPlot(pbmc3k,reduction = "pca_topics",pt.size = 1) + + theme_cowplot(font_size = 10) ``` Compare this against PCs 1 and 2 of the transformed counts: -```{r pca-2, fig.width=4, fig.height=3} +```{r pca-2, fig.width=4.5, fig.height=3, message=FALSE} pbmc3k <- FindVariableFeatures(pbmc3k) pbmc3k <- ScaleData(pbmc3k) pbmc3k <- RunPCA(pbmc3k) -DimPlot(pbmc3k,reduction = "pca",pt.size = 1) +DimPlot(pbmc3k,reduction = "pca",pt.size = 1) + + theme_cowplot(font_size = 10) ``` -Once fitted topic model is extracted, many functions from the -fastTopics package can be used for analysis and visualization. For +Once the fitted topic model is extracted, many functions from the +**fastTopics** package can be used for analysis and visualization. For example, the Structure plot provides an evocative visual summary of the estimated mixture proportions for each cell. -```{r structure-plot, fig.width=7.5, fig.height=2} +```{r structure-plot, fig.width=7.5, fig.height=1.75, results="hide", message=FALSE} fit <- Misc(Reductions(pbmc3k,"multinom_topic_model")) structure_plot(fit,grouping = Idents(pbmc3k),gap = 25) ``` @@ -91,3 +104,6 @@ these results. ```{r session-info} sessionInfo() ``` + +[fasttopics]: https://github.com/stephenslab/fastTopics +[countclust-paper]: https://doi.org/10.1371/journal.pgen.1006599 From 737a8fc784bc6779d40abb347f71556927f78b10 Mon Sep 17 00:00:00 2001 From: Peter Carbonetto Date: Fri, 15 Jan 2021 16:17:20 -0600 Subject: [PATCH 11/50] Made a few small revisions to the vignette. --- R/fasttopics.R | 7 +- docs/fasttopics.Rmd | 41 ++-- docs/fasttopics.html | 188 ++++++++++------- docs/fasttopics.md | 469 +++++++++++++++++++++++++++++++++++++------ 4 files changed, 542 insertions(+), 163 deletions(-) diff --git a/R/fasttopics.R b/R/fasttopics.R index fb09480..f9a6250 100644 --- a/R/fasttopics.R +++ b/R/fasttopics.R @@ -1,9 +1,4 @@ -# TO DO: -# -# - Implement FitPoissonNMF. -# -# - Implement vignette. -# +# TO DO: Implement FitPoissonNMF. #' @title Fit a Multinomial Topic Model Using fastTopics #' diff --git a/docs/fasttopics.Rmd b/docs/fasttopics.Rmd index 404e5c1..746e679 100644 --- a/docs/fasttopics.Rmd +++ b/docs/fasttopics.Rmd @@ -10,21 +10,24 @@ output: theme: readable --- -*Add introductory text here.* - -*Point to the fastTopics website and vignettes.* +Here we illustrate the use of the [fastTopics][fasttopics] Seurat +wrapper to analyze a Seurat data set. This vignette is only intended +to introduce the basic fastTopics interface for Seurat objects---for +background and practical guidance on analysis of single-cell RNA-seq +data using a topic model, please see the +[fastTopics vignettes][fasttopics-vignettes]. If you find the **fastTopics** package useful for your work, please cite: -> Kushal K. Dey, Chiaowen Joyce Hsiao and Matthew Stephens (2017). -> [Visualizing the structure of RNA-seq expression data using grade of membership models.][countclust-paper] -> *PLoS Genetics* **13**, e1006599. -> -> Peter Carbonetto, Kevin Luo, Kushal Dey, Joyce Hsiao and Matthew -> Stephens (2021). fastTopics: fast algorithms for fitting topic models -> and non-negative matrix factorizations to count data. R package -> version 0.4-11. [https://github.com/stephenslab/fastTopics][fasttopics] +Kushal K. Dey, Chiaowen Joyce Hsiao and Matthew Stephens (2017). +[Visualizing the structure of RNA-seq expression data using grade of +membership models.][countclust-paper] *PLoS Genetics* **13**, e1006599. + +Peter Carbonetto, Kevin Luo, Kushal Dey, Joyce Hsiao and Matthew +Stephens (2021). fastTopics: fast algorithms for fitting topic models +and non-negative matrix factorizations to count data. R package +version 0.4-23. [https://github.com/stephenslab/fastTopics][fasttopics] ```{r knitr-opts, include=FALSE} knitr::opts_chunk$set(comment = "#",collapse = TRUE,results = "hold", @@ -70,7 +73,7 @@ of $K$ will not be known in advance, so you will you want to explore the results from topic models at different settings of $K$. This plot shows the cells projected onto the top two principal -components (PCs) obtained from the topic mixture proportions. +components (PCs) of the topic mixture proportions. ```{r pca-1, fig.width=4.5, fig.height=3} Idents(pbmc3k) <- pbmc3k$seurat_annotations @@ -78,7 +81,7 @@ DimPlot(pbmc3k,reduction = "pca_topics",pt.size = 1) + theme_cowplot(font_size = 10) ``` -Compare this against PCs 1 and 2 of the transformed counts: +Compare this against the top two PCs of the transformed counts: ```{r pca-2, fig.width=4.5, fig.height=3, message=FALSE} pbmc3k <- FindVariableFeatures(pbmc3k) @@ -88,13 +91,20 @@ DimPlot(pbmc3k,reduction = "pca",pt.size = 1) + theme_cowplot(font_size = 10) ``` +The fitted topic model---a "multinom_topic_model" object---is stored +in the "misc" slot: + +```{r extract-fit} +fit <- Misc(Reductions(pbmc3k,"multinom_topic_model")) +``` + Once the fitted topic model is extracted, many functions from the **fastTopics** package can be used for analysis and visualization. For example, the Structure plot provides an evocative visual summary of -the estimated mixture proportions for each cell. +the estimated mixture proportions for each cell. Here, we have grouped +the cells by previously inferred labels. ```{r structure-plot, fig.width=7.5, fig.height=1.75, results="hide", message=FALSE} -fit <- Misc(Reductions(pbmc3k,"multinom_topic_model")) structure_plot(fit,grouping = Idents(pbmc3k),gap = 25) ``` @@ -106,4 +116,5 @@ sessionInfo() ``` [fasttopics]: https://github.com/stephenslab/fastTopics +[fasttopics-vignettes]: https://stephenslab.github.io/fastTopics/articles [countclust-paper]: https://doi.org/10.1371/journal.pgen.1006599 diff --git a/docs/fasttopics.html b/docs/fasttopics.html index bf898df..2b19dd3 100644 --- a/docs/fasttopics.html +++ b/docs/fasttopics.html @@ -11,7 +11,7 @@ -Add title here +Analyzing Seurat data using fastTopics - @@ -361,86 +371,110 @@ -

Add title here

+

Analyzing Seurat data using fastTopics

Compiled: January 15, 2021

-

Add introductory text here.

-

Add citation here.

+

Here we illustrate the use of the fastTopics Seurat wrapper to analyze a Seurat data set. This vignette is only intended to introduce the basic fastTopics interface for Seurat objects—for background and practical guidance on analysis of single-cell RNA-seq data using a topic model, please see the fastTopics vignettes.

+

If you find the fastTopics package useful for your work, please cite:

+

Kushal K. Dey, Chiaowen Joyce Hsiao and Matthew Stephens (2017). Visualizing the structure of RNA-seq expression data using grade of membership models. PLoS Genetics 13, e1006599.

+

Peter Carbonetto, Kevin Luo, Kushal Dey, Joyce Hsiao and Matthew Stephens (2021). fastTopics: fast algorithms for fitting topic models and non-negative matrix factorizations to count data. R package version 0.4-23. https://github.com/stephenslab/fastTopics

We begin by loading the packages used to perform the analysis.

library(Seurat)
 library(SeuratData)
 library(SeuratWrappers)
-library(fastTopics)
+library(fastTopics) +library(cowplot)

We set the seed so that the results can be reproduced.

set.seed(1)
-

Load—and, if necessary, install—the PBMC 3k data set.

-
InstallData("pbmc3k")
-
## Warning: The following packages are already installed and will not be
-## reinstalled: pbmc3k
-
data(pbmc3k)
+

Load—and, if necessary, install—the PBMC 3k data set containing transcription profiles for 2,700 cells.

+
InstallData("pbmc3k")
+data(pbmc3k)
+dim(GetAssayData(pbmc3k))
+# [1] 13714  2700
+

Fit the multinomial topic model to the raw UMI counts—no pre-processing or pre-selection of genes is needed. Note that it may take several minutes to complete this model fitting step.

+
pbmc3k <- FitTopicModel(pbmc3k,k = 6)
+

To fit a topic model, we must specify \(K\), the number of topics. Here, we have chosen \(K = 6\) topics. In most settings, a good choice of \(K\) will not be known in advance, so you will you want to explore the results from topic models at different settings of \(K\).

+

This plot shows the cells projected onto the top two principal components (PCs) of the topic mixture proportions.

+
Idents(pbmc3k) <- pbmc3k$seurat_annotations
+DimPlot(pbmc3k,reduction = "pca_topics",pt.size = 1) +
+  theme_cowplot(font_size = 10)
+

+

Compare this against the top two PCs of the transformed counts:

+
pbmc3k <- FindVariableFeatures(pbmc3k)
+pbmc3k <- ScaleData(pbmc3k)
+pbmc3k <- RunPCA(pbmc3k)
+DimPlot(pbmc3k,reduction = "pca",pt.size = 1) +
+  theme_cowplot(font_size = 10)
+

+

The fitted topic model—a “multinom_topic_model” object—is stored in the “misc” slot:

+
fit <- Misc(Reductions(pbmc3k,"multinom_topic_model"))
+

Once the fitted topic model is extracted, many functions from the fastTopics package can be used for analysis and visualization. For example, the Structure plot provides an evocative visual summary of the estimated mixture proportions for each cell. Here, we have grouped the cells by previously inferred labels.

+
structure_plot(fit,grouping = Idents(pbmc3k),gap = 25)
+

This is the version of R and the packages that were used to generate these results.

-
sessionInfo()
-
## R version 3.6.2 (2019-12-12)
-## Platform: x86_64-apple-darwin15.6.0 (64-bit)
-## Running under: macOS Catalina 10.15.7
-## 
-## Matrix products: default
-## BLAS:   /Library/Frameworks/R.framework/Versions/3.6/Resources/lib/libRblas.0.dylib
-## LAPACK: /Library/Frameworks/R.framework/Versions/3.6/Resources/lib/libRlapack.dylib
-## 
-## locale:
-## [1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8
-## 
-## attached base packages:
-## [1] stats     graphics  grDevices utils     datasets  methods   base     
-## 
-## other attached packages:
-## [1] fastTopics_0.4-23       SeuratWrappers_0.3.2    pbmc3k.SeuratData_3.1.4
-## [4] SeuratData_0.2.1        Seurat_3.2.3           
-## 
-## loaded via a namespace (and not attached):
-##   [1] Rtsne_0.15            colorspace_1.4-1      deldir_0.1-29        
-##   [4] ggridges_0.5.2        spatstat.data_1.4-3   leiden_0.3.3         
-##   [7] listenv_0.8.0         MatrixModels_0.4-1    remotes_2.1.0        
-##  [10] ggrepel_0.9.0         fansi_0.4.0           codetools_0.2-16     
-##  [13] splines_3.6.2         knitr_1.26            polyclip_1.10-0      
-##  [16] zeallot_0.1.0         jsonlite_1.6          mcmc_0.9-6           
-##  [19] ica_1.0-2             cluster_2.1.0         png_0.1-7            
-##  [22] uwot_0.1.10           shiny_1.4.0           sctransform_0.3.2    
-##  [25] BiocManager_1.30.10   compiler_3.6.2        httr_1.4.2           
-##  [28] backports_1.1.5       assertthat_0.2.1      Matrix_1.2-18        
-##  [31] fastmap_1.0.1         lazyeval_0.2.2        cli_2.0.0            
-##  [34] later_1.0.0           htmltools_0.4.0       quantreg_5.54        
-##  [37] prettyunits_1.1.1     tools_3.6.2           rsvd_1.0.2           
-##  [40] igraph_1.2.5          coda_0.19-3           gtable_0.3.0         
-##  [43] glue_1.3.1            RANN_2.6.1            reshape2_1.4.3       
-##  [46] dplyr_0.8.3           rappdirs_0.3.1        Rcpp_1.0.5           
-##  [49] spatstat_1.64-1       scattermore_0.7       vctrs_0.2.1          
-##  [52] nlme_3.1-142          lmtest_0.9-38         xfun_0.11            
-##  [55] stringr_1.4.0         globals_0.13.0        mime_0.8             
-##  [58] miniUI_0.1.1.1        lifecycle_0.1.0       irlba_2.3.3          
-##  [61] goftest_1.2-2         future_1.18.0         MASS_7.3-51.4        
-##  [64] zoo_1.8-7             scales_1.1.0          hms_0.5.2            
-##  [67] promises_1.1.0        spatstat.utils_1.17-0 parallel_3.6.2       
-##  [70] SparseM_1.78          RColorBrewer_1.1-2    yaml_2.2.0           
-##  [73] reticulate_1.16       pbapply_1.4-3         gridExtra_2.3        
-##  [76] ggplot2_3.3.0         rpart_4.1-15          stringi_1.4.3        
-##  [79] rlang_0.4.5           pkgconfig_2.0.3       matrixStats_0.56.0   
-##  [82] evaluate_0.14         lattice_0.20-38       ROCR_1.0-11          
-##  [85] purrr_0.3.3           tensor_1.5            patchwork_1.0.1      
-##  [88] htmlwidgets_1.5.1     cowplot_1.0.0         tidyselect_0.2.5     
-##  [91] RcppAnnoy_0.0.18      plyr_1.8.5            magrittr_1.5         
-##  [94] R6_2.4.1              pillar_1.4.3          mgcv_1.8-31          
-##  [97] fitdistrplus_1.1-1    survival_3.1-8        abind_1.4-5          
-## [100] tibble_2.1.3          future.apply_1.6.0    crayon_1.3.4         
-## [103] KernSmooth_2.23-16    plotly_4.9.2          rmarkdown_2.3        
-## [106] progress_1.2.2        grid_3.6.2            data.table_1.12.8    
-## [109] digest_0.6.23         xtable_1.8-4          tidyr_1.0.0          
-## [112] httpuv_1.5.2          MCMCpack_1.4-5        RcppParallel_4.4.2   
-## [115] munsell_0.5.0         viridisLite_0.3.0     quadprog_1.5-8
+
sessionInfo()
+# R version 3.6.2 (2019-12-12)
+# Platform: x86_64-apple-darwin15.6.0 (64-bit)
+# Running under: macOS Catalina 10.15.7
+# 
+# Matrix products: default
+# BLAS:   /Library/Frameworks/R.framework/Versions/3.6/Resources/lib/libRblas.0.dylib
+# LAPACK: /Library/Frameworks/R.framework/Versions/3.6/Resources/lib/libRlapack.dylib
+# 
+# locale:
+# [1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8
+# 
+# attached base packages:
+# [1] stats     graphics  grDevices utils     datasets  methods   base     
+# 
+# other attached packages:
+# [1] cowplot_1.0.0           fastTopics_0.4-23       SeuratWrappers_0.3.2   
+# [4] pbmc3k.SeuratData_3.1.4 SeuratData_0.2.1        Seurat_3.2.3           
+# 
+# loaded via a namespace (and not attached):
+#   [1] Rtsne_0.15            colorspace_1.4-1      deldir_0.1-29        
+#   [4] ggridges_0.5.2        spatstat.data_1.4-3   farver_2.0.1         
+#   [7] leiden_0.3.3          listenv_0.8.0         remotes_2.1.0        
+#  [10] MatrixModels_0.4-1    ggrepel_0.9.0         fansi_0.4.0          
+#  [13] codetools_0.2-16      splines_3.6.2         knitr_1.26           
+#  [16] polyclip_1.10-0       zeallot_0.1.0         jsonlite_1.6         
+#  [19] mcmc_0.9-6            ica_1.0-2             cluster_2.1.0        
+#  [22] png_0.1-7             uwot_0.1.10           shiny_1.4.0          
+#  [25] sctransform_0.3.2     BiocManager_1.30.10   compiler_3.6.2       
+#  [28] httr_1.4.2            backports_1.1.5       assertthat_0.2.1     
+#  [31] Matrix_1.2-18         fastmap_1.0.1         lazyeval_0.2.2       
+#  [34] cli_2.0.0             later_1.0.0           prettyunits_1.1.1    
+#  [37] htmltools_0.4.0       quantreg_5.54         tools_3.6.2          
+#  [40] rsvd_1.0.2            igraph_1.2.5          coda_0.19-3          
+#  [43] gtable_0.3.0          glue_1.3.1            RANN_2.6.1           
+#  [46] reshape2_1.4.3        dplyr_0.8.3           rappdirs_0.3.1       
+#  [49] Rcpp_1.0.5            spatstat_1.64-1       scattermore_0.7      
+#  [52] vctrs_0.2.1           nlme_3.1-142          lmtest_0.9-38        
+#  [55] xfun_0.11             stringr_1.4.0         globals_0.13.0       
+#  [58] mime_0.8              miniUI_0.1.1.1        lifecycle_0.1.0      
+#  [61] irlba_2.3.3           goftest_1.2-2         future_1.18.0        
+#  [64] MASS_7.3-51.4         zoo_1.8-7             scales_1.1.0         
+#  [67] hms_0.5.2             promises_1.1.0        spatstat.utils_1.17-0
+#  [70] parallel_3.6.2        SparseM_1.78          RColorBrewer_1.1-2   
+#  [73] yaml_2.2.0            reticulate_1.16       pbapply_1.4-3        
+#  [76] gridExtra_2.3         ggplot2_3.3.0         rpart_4.1-15         
+#  [79] stringi_1.4.3         rlang_0.4.5           pkgconfig_2.0.3      
+#  [82] matrixStats_0.56.0    evaluate_0.14         lattice_0.20-38      
+#  [85] ROCR_1.0-11           purrr_0.3.3           tensor_1.5           
+#  [88] labeling_0.3          patchwork_1.0.1       htmlwidgets_1.5.1    
+#  [91] tidyselect_0.2.5      RcppAnnoy_0.0.18      plyr_1.8.5           
+#  [94] magrittr_1.5          R6_2.4.1              pillar_1.4.3         
+#  [97] mgcv_1.8-31           fitdistrplus_1.1-1    survival_3.1-8       
+# [100] abind_1.4-5           tibble_2.1.3          future.apply_1.6.0   
+# [103] crayon_1.3.4          KernSmooth_2.23-16    plotly_4.9.2         
+# [106] rmarkdown_2.3         progress_1.2.2        grid_3.6.2           
+# [109] data.table_1.12.8     digest_0.6.23         xtable_1.8-4         
+# [112] tidyr_1.0.0           httpuv_1.5.2          MCMCpack_1.4-5       
+# [115] RcppParallel_4.4.2    munsell_0.5.0         viridisLite_0.3.0    
+# [118] quadprog_1.5-8
diff --git a/docs/fasttopics.md b/docs/fasttopics.md index a93f4a0..d2aef84 100644 --- a/docs/fasttopics.md +++ b/docs/fasttopics.md @@ -1,9 +1,11 @@ -Add title here +Analyzing Seurat data using fastTopics ================ Compiled: January 15, 2021 *Add introductory text here.* +*Point to the fastTopics website and vignettes.* + *Add citation here.* We begin by loading the packages used to perform the analysis. @@ -21,82 +23,419 @@ We set the seed so that the results can be reproduced. set.seed(1) ``` -Load—and, if necessary, install—the PBMC 3k data - set. +Load—and, if necessary, install—the PBMC 3k data set containing +transcription profiles for 2,700 cells. ``` r InstallData("pbmc3k") +data(pbmc3k) +dim(GetAssayData(pbmc3k)) +# [1] 13714 2700 ``` - ## Warning: The following packages are already installed and will not be - ## reinstalled: pbmc3k +Fit the multinomial topic model to the raw UMI counts—*no pre-processing +or pre-selection of genes is needed.* Note that it may take several +minutes to complete this model fitting step. ``` r -data(pbmc3k) +pbmc3k <- FitTopicModel(pbmc3k,k = 6) ``` +To fit a topic model, we must specify \(K\), the number of topics. Here, +we have chosen \(K = 6\) topics. In most settings, a good choice of +\(K\) will not be known in advance, so you will you want to explore the +results from topic models at different settings of \(K\). + +This plot shows the cells projected onto the top two principal +components (PCs) obtained from the topic mixture proportions. + +``` r +Idents(pbmc3k) <- pbmc3k$seurat_annotations +DimPlot(pbmc3k,reduction = "pca_topics",pt.size = 1) +``` + + + +Compare this against PCs 1 and 2 of the transformed counts: + +``` r +pbmc3k <- FindVariableFeatures(pbmc3k) +pbmc3k <- ScaleData(pbmc3k) +# Centering and scaling data matrix +pbmc3k <- RunPCA(pbmc3k) +# PC_ 1 +# Positive: FTL, FTH1, COTL1, CST3, OAZ1, ACTB, LGALS1, S100A4, AIF1, FCER1G +# TMSB4X, S100A6, TYROBP, LST1, PSAP, TYMP, SAT1, S100A11, CTSS, SPI1 +# SERPINA1, LYZ, TMSB10, IFITM3, HLA-DRB1, FCN1, CFD, HLA-DPA1, VIM, GSTP1 +# Negative: MALAT1, IL32, LTB, CCL5, CTSW, CD247, CD2, NKG7, LINC00926, ACAP1 +# TCL1A, CST7, GZMA, FGFBP2, NCR3, BEX2, GZMK, HOPX, GNLY, MAL +# SAMD3, STK17A, MYC, XCL1, SPON2, NELL2, LDLRAP1, ZAP70, XCL2, PRF1 +# PC_ 2 +# Positive: FTL, TYROBP, S100A8, S100A9, FCN1, AIF1, FTH1, LYZ, CTSS, LST1 +# CFD, S100A6, TYMP, SAT1, SERPINA1, LGALS2, S100A11, PSAP, S100A4, CST3 +# FCER1G, IFITM3, CFP, APOBEC3A, LGALS1, SPI1, LGALS3, TIMP1, NPC2, IFI30 +# Negative: ACTG1, STMN1, TUBA1B, TYMS, ZWINT, GZMA, KIAA0101, TK1, RRM2, HMGB2 +# H2AFZ, DUT, HMGA1, FEN1, MYBL2, BIRC5, GINS2, GAPDH, SRSF3, NKG7 +# HSP90AA1, PTTG1, IL32, FABP5, ASF1B, MKI67, RANBP1, KIFC1, CENPM, ACTB +# PC_ 3 +# Positive: CD74, HLA-DRA, HLA-DPB1, HLA-DQB1, HLA-DQA1, HLA-DRB1, HLA-DPA1, HLA-DQA2, HLA-DRB5, LYZ +# HLA-DMB, CD1C, CST3, VIM, LGALS2, HLA-DMA, MS4A1, CD79A, CLEC10A, LINC00926 +# CD79B, GSTP1, MS4A6A, TCL1A, S100A9, FCER1A, PLD4, FCN1, S100A8, IFI6 +# Negative: PPBP, GNG11, SPARC, PF4, AP001189.4, SDPR, ITGA2B, CLU, CD9, TREML1 +# GP9, LY6G6F, CMTM5, NRGN, TUBB1, RP11-367G6.3, C6orf25, GP1BA, RGS18, F13A1 +# CA2, SCGB1C1, RUFY1, CLDN5, SEPT5, CLEC1B, ITGB3, HIST1H2AC, TUBA4A, CTTN +# PC_ 4 +# Positive: CD74, HLA-DQB1, HLA-DQA1, HLA-DRA, HLA-DQA2, HLA-DPB1, CD79A, HLA-DRB1, MS4A1, HLA-DPA1 +# HLA-DRB5, CD79B, HLA-DMB, CD1C, LINC00926, GPX1, TCL1A, HLA-DMA, KIAA0125, PPBP +# CLEC10A, P2RX5, PPP1R14A, PF4, PLD4, SPARC, FCER1A, GNG11, ITM2C, ITGA2B +# Negative: NKG7, GZMA, GNLY, PRF1, FGFBP2, SPON2, CTSW, FCGR3A, CST7, GZMB +# AKR1C3, CLIC3, GZMH, CCL5, CD247, XCL2, HOPX, IFITM2, CCL4, XCL1 +# FCER1G, TTC38, S100A4, CTSC, IL32, LST1, MS4A7, SERPINA1, AIF1, SAMD3 +# PC_ 5 +# Positive: ZWINT, KIAA0101, RRM2, HMGB2, AQP3, FEN1, TYMS, H2AFZ, GINS2, KIFC1 +# TK1, MYBL2, HN1, BIRC5, LTB, S100A8, FTL, NUSAP1, CDC45, CKS1B +# DUT, CENPM, MKI67, STMN1, CDCA5, ASF1B, S100A9, GAPDH, CFD, AIF1 +# Negative: NKG7, CD74, HLA-DQA1, GNLY, SPON2, HLA-DPB1, FGFBP2, HLA-DQA2, HLA-DRA, GZMB +# HLA-DQB1, PRF1, HLA-DPA1, HLA-DRB1, GZMA, CTSW, HLA-DRB5, CLIC3, AKR1C3, CST7 +# CD1C, XCL2, CCL4, XCL1, HOPX, CCL5, CLEC10A, HLA-DMB, TTC38, FCER1A +DimPlot(pbmc3k,reduction = "pca",pt.size = 1) +``` + + + +Once fitted topic model is extracted, many functions from the fastTopics +package can be used for analysis and visualization. For example, the +Structure plot provides an evocative visual summary of the estimated +mixture proportions for each cell. + +``` r +fit <- Misc(Reductions(pbmc3k,"multinom_topic_model")) +structure_plot(fit,grouping = Idents(pbmc3k),gap = 25) +# Perplexity automatically changed to 83 because original setting of 100 was too large for the number of samples (255) +# Perplexity automatically changed to 65 because original setting of 100 was too large for the number of samples (201) +# Perplexity automatically changed to 38 because original setting of 100 was too large for the number of samples (120) +# Perplexity automatically changed to 37 because original setting of 100 was too large for the number of samples (115) +# Perplexity automatically changed to 6 because original setting of 100 was too large for the number of samples (24) +# Perplexity automatically changed to 2 because original setting of 100 was too large for the number of samples (10) +``` + + + + # Read the 516 x 6 data matrix successfully! + # OpenMP is working. 1 threads. + # Using no_dims = 1, perplexity = 100.000000, and theta = 0.100000 + # Computing input similarities... + # Building tree... + # Done in 0.15 seconds (sparsity = 0.725084)! + # Learning embedding... + # Iteration 50: error is 46.544798 (50 iterations in 0.07 seconds) + # Iteration 100: error is 44.191974 (50 iterations in 0.06 seconds) + # Iteration 150: error is 44.191098 (50 iterations in 0.06 seconds) + # Iteration 200: error is 44.191097 (50 iterations in 0.06 seconds) + # Iteration 250: error is 44.191097 (50 iterations in 0.05 seconds) + # Iteration 300: error is 0.267504 (50 iterations in 0.06 seconds) + # Iteration 350: error is 0.265744 (50 iterations in 0.06 seconds) + # Iteration 400: error is 0.265741 (50 iterations in 0.06 seconds) + # Iteration 450: error is 0.265742 (50 iterations in 0.06 seconds) + # Iteration 500: error is 0.265742 (50 iterations in 0.06 seconds) + # Iteration 550: error is 0.265742 (50 iterations in 0.06 seconds) + # Iteration 600: error is 0.265742 (50 iterations in 0.05 seconds) + # Iteration 650: error is 0.265742 (50 iterations in 0.06 seconds) + # Iteration 700: error is 0.265742 (50 iterations in 0.06 seconds) + # Iteration 750: error is 0.265742 (50 iterations in 0.06 seconds) + # Iteration 800: error is 0.265742 (50 iterations in 0.06 seconds) + # Iteration 850: error is 0.265742 (50 iterations in 0.06 seconds) + # Iteration 900: error is 0.265742 (50 iterations in 0.06 seconds) + # Iteration 950: error is 0.265742 (50 iterations in 0.06 seconds) + # Iteration 1000: error is 0.265742 (50 iterations in 0.06 seconds) + # Fitting performed in 1.14 seconds. + # Read the 358 x 6 data matrix successfully! + # OpenMP is working. 1 threads. + # Using no_dims = 1, perplexity = 100.000000, and theta = 0.100000 + # Computing input similarities... + # Building tree... + # Done in 0.10 seconds (sparsity = 0.948269)! + # Learning embedding... + # Iteration 50: error is 42.965050 (50 iterations in 0.04 seconds) + # Iteration 100: error is 41.889224 (50 iterations in 0.04 seconds) + # Iteration 150: error is 41.888879 (50 iterations in 0.04 seconds) + # Iteration 200: error is 41.888918 (50 iterations in 0.04 seconds) + # Iteration 250: error is 41.888924 (50 iterations in 0.04 seconds) + # Iteration 300: error is 0.154191 (50 iterations in 0.04 seconds) + # Iteration 350: error is 0.154156 (50 iterations in 0.04 seconds) + # Iteration 400: error is 0.154155 (50 iterations in 0.04 seconds) + # Iteration 450: error is 0.154155 (50 iterations in 0.04 seconds) + # Iteration 500: error is 0.154155 (50 iterations in 0.04 seconds) + # Iteration 550: error is 0.154155 (50 iterations in 0.04 seconds) + # Iteration 600: error is 0.154155 (50 iterations in 0.04 seconds) + # Iteration 650: error is 0.154155 (50 iterations in 0.04 seconds) + # Iteration 700: error is 0.154155 (50 iterations in 0.04 seconds) + # Iteration 750: error is 0.154155 (50 iterations in 0.04 seconds) + # Iteration 800: error is 0.154155 (50 iterations in 0.04 seconds) + # Iteration 850: error is 0.154155 (50 iterations in 0.04 seconds) + # Iteration 900: error is 0.154155 (50 iterations in 0.04 seconds) + # Iteration 950: error is 0.154155 (50 iterations in 0.04 seconds) + # Iteration 1000: error is 0.154155 (50 iterations in 0.04 seconds) + # Fitting performed in 0.73 seconds. + # Read the 356 x 6 data matrix successfully! + # OpenMP is working. 1 threads. + # Using no_dims = 1, perplexity = 100.000000, and theta = 0.100000 + # Computing input similarities... + # Building tree... + # Done in 0.10 seconds (sparsity = 0.948886)! + # Learning embedding... + # Iteration 50: error is 44.127036 (50 iterations in 0.04 seconds) + # Iteration 100: error is 42.960180 (50 iterations in 0.04 seconds) + # Iteration 150: error is 42.946318 (50 iterations in 0.03 seconds) + # Iteration 200: error is 42.946371 (50 iterations in 0.04 seconds) + # Iteration 250: error is 42.946352 (50 iterations in 0.04 seconds) + # Iteration 300: error is 0.264561 (50 iterations in 0.04 seconds) + # Iteration 350: error is 0.264515 (50 iterations in 0.03 seconds) + # Iteration 400: error is 0.264520 (50 iterations in 0.04 seconds) + # Iteration 450: error is 0.264520 (50 iterations in 0.03 seconds) + # Iteration 500: error is 0.264520 (50 iterations in 0.03 seconds) + # Iteration 550: error is 0.264520 (50 iterations in 0.04 seconds) + # Iteration 600: error is 0.264520 (50 iterations in 0.04 seconds) + # Iteration 650: error is 0.264520 (50 iterations in 0.03 seconds) + # Iteration 700: error is 0.264520 (50 iterations in 0.04 seconds) + # Iteration 750: error is 0.264520 (50 iterations in 0.03 seconds) + # Iteration 800: error is 0.264520 (50 iterations in 0.03 seconds) + # Iteration 850: error is 0.264520 (50 iterations in 0.03 seconds) + # Iteration 900: error is 0.264520 (50 iterations in 0.03 seconds) + # Iteration 950: error is 0.264520 (50 iterations in 0.03 seconds) + # Iteration 1000: error is 0.264520 (50 iterations in 0.03 seconds) + # Fitting performed in 0.71 seconds. + # Read the 255 x 6 data matrix successfully! + # OpenMP is working. 1 threads. + # Using no_dims = 1, perplexity = 83.000000, and theta = 0.100000 + # Computing input similarities... + # Building tree... + # Done in 0.05 seconds (sparsity = 0.995309)! + # Learning embedding... + # Iteration 50: error is 41.894862 (50 iterations in 0.03 seconds) + # Iteration 100: error is 41.348727 (50 iterations in 0.02 seconds) + # Iteration 150: error is 41.316669 (50 iterations in 0.02 seconds) + # Iteration 200: error is 41.331596 (50 iterations in 0.02 seconds) + # Iteration 250: error is 41.337796 (50 iterations in 0.02 seconds) + # Iteration 300: error is 0.153763 (50 iterations in 0.02 seconds) + # Iteration 350: error is 0.152951 (50 iterations in 0.02 seconds) + # Iteration 400: error is 0.152945 (50 iterations in 0.02 seconds) + # Iteration 450: error is 0.152945 (50 iterations in 0.02 seconds) + # Iteration 500: error is 0.152945 (50 iterations in 0.02 seconds) + # Iteration 550: error is 0.152945 (50 iterations in 0.02 seconds) + # Iteration 600: error is 0.152945 (50 iterations in 0.02 seconds) + # Iteration 650: error is 0.152947 (50 iterations in 0.02 seconds) + # Iteration 700: error is 0.152948 (50 iterations in 0.02 seconds) + # Iteration 750: error is 0.152947 (50 iterations in 0.02 seconds) + # Iteration 800: error is 0.152945 (50 iterations in 0.02 seconds) + # Iteration 850: error is 0.152945 (50 iterations in 0.02 seconds) + # Iteration 900: error is 0.152947 (50 iterations in 0.02 seconds) + # Iteration 950: error is 0.152945 (50 iterations in 0.02 seconds) + # Iteration 1000: error is 0.152947 (50 iterations in 0.02 seconds) + # Fitting performed in 0.46 seconds. + # Read the 201 x 6 data matrix successfully! + # OpenMP is working. 1 threads. + # Using no_dims = 1, perplexity = 65.000000, and theta = 0.100000 + # Computing input similarities... + # Building tree... + # Done in 0.03 seconds (sparsity = 0.993688)! + # Learning embedding... + # Iteration 50: error is 43.455428 (50 iterations in 0.02 seconds) + # Iteration 100: error is 42.659459 (50 iterations in 0.02 seconds) + # Iteration 150: error is 42.729750 (50 iterations in 0.02 seconds) + # Iteration 200: error is 43.218125 (50 iterations in 0.02 seconds) + # Iteration 250: error is 43.736809 (50 iterations in 0.02 seconds) + # Iteration 300: error is 0.664267 (50 iterations in 0.02 seconds) + # Iteration 350: error is 0.649046 (50 iterations in 0.02 seconds) + # Iteration 400: error is 0.581421 (50 iterations in 0.02 seconds) + # Iteration 450: error is 0.581122 (50 iterations in 0.02 seconds) + # Iteration 500: error is 0.581124 (50 iterations in 0.02 seconds) + # Iteration 550: error is 0.581123 (50 iterations in 0.02 seconds) + # Iteration 600: error is 0.581120 (50 iterations in 0.02 seconds) + # Iteration 650: error is 0.581120 (50 iterations in 0.02 seconds) + # Iteration 700: error is 0.581120 (50 iterations in 0.02 seconds) + # Iteration 750: error is 0.581124 (50 iterations in 0.02 seconds) + # Iteration 800: error is 0.581124 (50 iterations in 0.02 seconds) + # Iteration 850: error is 0.581123 (50 iterations in 0.02 seconds) + # Iteration 900: error is 0.581124 (50 iterations in 0.02 seconds) + # Iteration 950: error is 0.581120 (50 iterations in 0.02 seconds) + # Iteration 1000: error is 0.581120 (50 iterations in 0.02 seconds) + # Fitting performed in 0.35 seconds. + # Read the 120 x 6 data matrix successfully! + # OpenMP is working. 1 threads. + # Using no_dims = 1, perplexity = 38.000000, and theta = 0.100000 + # Computing input similarities... + # Building tree... + # Done in 0.01 seconds (sparsity = 0.988750)! + # Learning embedding... + # Iteration 50: error is 45.952359 (50 iterations in 0.01 seconds) + # Iteration 100: error is 48.966439 (50 iterations in 0.01 seconds) + # Iteration 150: error is 48.365154 (50 iterations in 0.01 seconds) + # Iteration 200: error is 50.364318 (50 iterations in 0.01 seconds) + # Iteration 250: error is 50.683345 (50 iterations in 0.01 seconds) + # Iteration 300: error is 1.136895 (50 iterations in 0.01 seconds) + # Iteration 350: error is 0.733483 (50 iterations in 0.01 seconds) + # Iteration 400: error is 0.625430 (50 iterations in 0.01 seconds) + # Iteration 450: error is 0.625001 (50 iterations in 0.01 seconds) + # Iteration 500: error is 0.624999 (50 iterations in 0.01 seconds) + # Iteration 550: error is 0.624999 (50 iterations in 0.01 seconds) + # Iteration 600: error is 0.624999 (50 iterations in 0.01 seconds) + # Iteration 650: error is 0.624986 (50 iterations in 0.01 seconds) + # Iteration 700: error is 0.624986 (50 iterations in 0.01 seconds) + # Iteration 750: error is 0.625000 (50 iterations in 0.01 seconds) + # Iteration 800: error is 0.624986 (50 iterations in 0.01 seconds) + # Iteration 850: error is 0.624986 (50 iterations in 0.01 seconds) + # Iteration 900: error is 0.625000 (50 iterations in 0.01 seconds) + # Iteration 950: error is 0.625000 (50 iterations in 0.01 seconds) + # Iteration 1000: error is 0.624999 (50 iterations in 0.01 seconds) + # Fitting performed in 0.17 seconds. + # Read the 115 x 6 data matrix successfully! + # OpenMP is working. 1 threads. + # Using no_dims = 1, perplexity = 37.000000, and theta = 0.100000 + # Computing input similarities... + # Building tree... + # Done in 0.01 seconds (sparsity = 0.989792)! + # Learning embedding... + # Iteration 50: error is 49.065562 (50 iterations in 0.01 seconds) + # Iteration 100: error is 49.210910 (50 iterations in 0.01 seconds) + # Iteration 150: error is 49.974686 (50 iterations in 0.01 seconds) + # Iteration 200: error is 51.228638 (50 iterations in 0.01 seconds) + # Iteration 250: error is 49.220168 (50 iterations in 0.01 seconds) + # Iteration 300: error is 1.749707 (50 iterations in 0.01 seconds) + # Iteration 350: error is 1.094096 (50 iterations in 0.01 seconds) + # Iteration 400: error is 0.868036 (50 iterations in 0.01 seconds) + # Iteration 450: error is 0.845715 (50 iterations in 0.01 seconds) + # Iteration 500: error is 0.845736 (50 iterations in 0.01 seconds) + # Iteration 550: error is 0.845737 (50 iterations in 0.01 seconds) + # Iteration 600: error is 0.845737 (50 iterations in 0.01 seconds) + # Iteration 650: error is 0.845737 (50 iterations in 0.01 seconds) + # Iteration 700: error is 0.845737 (50 iterations in 0.01 seconds) + # Iteration 750: error is 0.845736 (50 iterations in 0.01 seconds) + # Iteration 800: error is 0.845736 (50 iterations in 0.01 seconds) + # Iteration 850: error is 0.845737 (50 iterations in 0.01 seconds) + # Iteration 900: error is 0.845737 (50 iterations in 0.01 seconds) + # Iteration 950: error is 0.845737 (50 iterations in 0.01 seconds) + # Iteration 1000: error is 0.845736 (50 iterations in 0.01 seconds) + # Fitting performed in 0.16 seconds. + # Read the 24 x 6 data matrix successfully! + # OpenMP is working. 1 threads. + # Using no_dims = 1, perplexity = 6.000000, and theta = 0.100000 + # Computing input similarities... + # Building tree... + # Done in 0.00 seconds (sparsity = 0.881944)! + # Learning embedding... + # Iteration 50: error is 70.976567 (50 iterations in 0.00 seconds) + # Iteration 100: error is 72.024095 (50 iterations in 0.00 seconds) + # Iteration 150: error is 72.360917 (50 iterations in 0.00 seconds) + # Iteration 200: error is 71.631815 (50 iterations in 0.00 seconds) + # Iteration 250: error is 73.191667 (50 iterations in 0.00 seconds) + # Iteration 300: error is 6.587625 (50 iterations in 0.00 seconds) + # Iteration 350: error is 3.246346 (50 iterations in 0.00 seconds) + # Iteration 400: error is 2.966947 (50 iterations in 0.00 seconds) + # Iteration 450: error is 2.960004 (50 iterations in 0.00 seconds) + # Iteration 500: error is 2.959519 (50 iterations in 0.00 seconds) + # Iteration 550: error is 2.959505 (50 iterations in 0.00 seconds) + # Iteration 600: error is 2.959505 (50 iterations in 0.00 seconds) + # Iteration 650: error is 2.959505 (50 iterations in 0.00 seconds) + # Iteration 700: error is 2.959505 (50 iterations in 0.00 seconds) + # Iteration 750: error is 2.959504 (50 iterations in 0.00 seconds) + # Iteration 800: error is 2.959503 (50 iterations in 0.00 seconds) + # Iteration 850: error is 2.959503 (50 iterations in 0.00 seconds) + # Iteration 900: error is 2.959502 (50 iterations in 0.00 seconds) + # Iteration 950: error is 2.959501 (50 iterations in 0.00 seconds) + # Iteration 1000: error is 2.959500 (50 iterations in 0.00 seconds) + # Fitting performed in 0.02 seconds. + # Read the 10 x 6 data matrix successfully! + # OpenMP is working. 1 threads. + # Using no_dims = 1, perplexity = 2.000000, and theta = 0.100000 + # Computing input similarities... + # Building tree... + # Done in 0.00 seconds (sparsity = 0.760000)! + # Learning embedding... + # Iteration 50: error is 91.006955 (50 iterations in 0.00 seconds) + # Iteration 100: error is 69.804871 (50 iterations in 0.00 seconds) + # Iteration 150: error is 82.678065 (50 iterations in 0.00 seconds) + # Iteration 200: error is 82.559507 (50 iterations in 0.00 seconds) + # Iteration 250: error is 130.669903 (50 iterations in 0.00 seconds) + # Iteration 300: error is 2.313263 (50 iterations in 0.00 seconds) + # Iteration 350: error is 2.268197 (50 iterations in 0.00 seconds) + # Iteration 400: error is 2.263634 (50 iterations in 0.00 seconds) + # Iteration 450: error is 2.262503 (50 iterations in 0.00 seconds) + # Iteration 500: error is 2.262447 (50 iterations in 0.00 seconds) + # Iteration 550: error is 2.262446 (50 iterations in 0.00 seconds) + # Iteration 600: error is 2.262446 (50 iterations in 0.00 seconds) + # Iteration 650: error is 2.262446 (50 iterations in 0.00 seconds) + # Iteration 700: error is 2.262446 (50 iterations in 0.00 seconds) + # Iteration 750: error is 2.262446 (50 iterations in 0.00 seconds) + # Iteration 800: error is 2.262446 (50 iterations in 0.00 seconds) + # Iteration 850: error is 2.262446 (50 iterations in 0.00 seconds) + # Iteration 900: error is 2.262446 (50 iterations in 0.00 seconds) + # Iteration 950: error is 2.262446 (50 iterations in 0.00 seconds) + # Iteration 1000: error is 2.262446 (50 iterations in 0.00 seconds) + # Fitting performed in 0.00 seconds. + This is the version of R and the packages that were used to generate these results. ``` r sessionInfo() +# R version 3.6.2 (2019-12-12) +# Platform: x86_64-apple-darwin15.6.0 (64-bit) +# Running under: macOS Catalina 10.15.7 +# +# Matrix products: default +# BLAS: /Library/Frameworks/R.framework/Versions/3.6/Resources/lib/libRblas.0.dylib +# LAPACK: /Library/Frameworks/R.framework/Versions/3.6/Resources/lib/libRlapack.dylib +# +# locale: +# [1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8 +# +# attached base packages: +# [1] stats graphics grDevices utils datasets methods base +# +# other attached packages: +# [1] fastTopics_0.4-23 SeuratWrappers_0.3.2 pbmc3k.SeuratData_3.1.4 +# [4] SeuratData_0.2.1 Seurat_3.2.3 +# +# loaded via a namespace (and not attached): +# [1] Rtsne_0.15 colorspace_1.4-1 deldir_0.1-29 +# [4] ggridges_0.5.2 spatstat.data_1.4-3 farver_2.0.1 +# [7] leiden_0.3.3 listenv_0.8.0 remotes_2.1.0 +# [10] MatrixModels_0.4-1 ggrepel_0.9.0 fansi_0.4.0 +# [13] codetools_0.2-16 splines_3.6.2 knitr_1.26 +# [16] polyclip_1.10-0 zeallot_0.1.0 jsonlite_1.6 +# [19] mcmc_0.9-6 ica_1.0-2 cluster_2.1.0 +# [22] png_0.1-7 uwot_0.1.10 shiny_1.4.0 +# [25] sctransform_0.3.2 BiocManager_1.30.10 compiler_3.6.2 +# [28] httr_1.4.2 backports_1.1.5 assertthat_0.2.1 +# [31] Matrix_1.2-18 fastmap_1.0.1 lazyeval_0.2.2 +# [34] cli_2.0.0 later_1.0.0 prettyunits_1.1.1 +# [37] htmltools_0.4.0 quantreg_5.54 tools_3.6.2 +# [40] rsvd_1.0.2 igraph_1.2.5 coda_0.19-3 +# [43] gtable_0.3.0 glue_1.3.1 RANN_2.6.1 +# [46] reshape2_1.4.3 dplyr_0.8.3 rappdirs_0.3.1 +# [49] Rcpp_1.0.5 spatstat_1.64-1 scattermore_0.7 +# [52] vctrs_0.2.1 nlme_3.1-142 lmtest_0.9-38 +# [55] xfun_0.11 stringr_1.4.0 globals_0.13.0 +# [58] mime_0.8 miniUI_0.1.1.1 lifecycle_0.1.0 +# [61] irlba_2.3.3 goftest_1.2-2 future_1.18.0 +# [64] MASS_7.3-51.4 zoo_1.8-7 scales_1.1.0 +# [67] hms_0.5.2 promises_1.1.0 spatstat.utils_1.17-0 +# [70] parallel_3.6.2 SparseM_1.78 RColorBrewer_1.1-2 +# [73] yaml_2.2.0 reticulate_1.16 pbapply_1.4-3 +# [76] gridExtra_2.3 ggplot2_3.3.0 rpart_4.1-15 +# [79] stringi_1.4.3 rlang_0.4.5 pkgconfig_2.0.3 +# [82] matrixStats_0.56.0 evaluate_0.14 lattice_0.20-38 +# [85] ROCR_1.0-11 purrr_0.3.3 tensor_1.5 +# [88] labeling_0.3 patchwork_1.0.1 htmlwidgets_1.5.1 +# [91] cowplot_1.0.0 tidyselect_0.2.5 RcppAnnoy_0.0.18 +# [94] plyr_1.8.5 magrittr_1.5 R6_2.4.1 +# [97] pillar_1.4.3 mgcv_1.8-31 fitdistrplus_1.1-1 +# [100] survival_3.1-8 abind_1.4-5 tibble_2.1.3 +# [103] future.apply_1.6.0 crayon_1.3.4 KernSmooth_2.23-16 +# [106] plotly_4.9.2 rmarkdown_2.3 progress_1.2.2 +# [109] grid_3.6.2 data.table_1.12.8 digest_0.6.23 +# [112] xtable_1.8-4 tidyr_1.0.0 httpuv_1.5.2 +# [115] MCMCpack_1.4-5 RcppParallel_4.4.2 munsell_0.5.0 +# [118] viridisLite_0.3.0 quadprog_1.5-8 ``` - - ## R version 3.6.2 (2019-12-12) - ## Platform: x86_64-apple-darwin15.6.0 (64-bit) - ## Running under: macOS Catalina 10.15.7 - ## - ## Matrix products: default - ## BLAS: /Library/Frameworks/R.framework/Versions/3.6/Resources/lib/libRblas.0.dylib - ## LAPACK: /Library/Frameworks/R.framework/Versions/3.6/Resources/lib/libRlapack.dylib - ## - ## locale: - ## [1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8 - ## - ## attached base packages: - ## [1] stats graphics grDevices utils datasets methods base - ## - ## other attached packages: - ## [1] fastTopics_0.4-23 SeuratWrappers_0.3.2 pbmc3k.SeuratData_3.1.4 - ## [4] SeuratData_0.2.1 Seurat_3.2.3 - ## - ## loaded via a namespace (and not attached): - ## [1] Rtsne_0.15 colorspace_1.4-1 deldir_0.1-29 - ## [4] ggridges_0.5.2 spatstat.data_1.4-3 leiden_0.3.3 - ## [7] listenv_0.8.0 MatrixModels_0.4-1 remotes_2.1.0 - ## [10] ggrepel_0.9.0 fansi_0.4.0 codetools_0.2-16 - ## [13] splines_3.6.2 knitr_1.26 polyclip_1.10-0 - ## [16] zeallot_0.1.0 jsonlite_1.6 mcmc_0.9-6 - ## [19] ica_1.0-2 cluster_2.1.0 png_0.1-7 - ## [22] uwot_0.1.10 shiny_1.4.0 sctransform_0.3.2 - ## [25] BiocManager_1.30.10 compiler_3.6.2 httr_1.4.2 - ## [28] backports_1.1.5 assertthat_0.2.1 Matrix_1.2-18 - ## [31] fastmap_1.0.1 lazyeval_0.2.2 cli_2.0.0 - ## [34] later_1.0.0 htmltools_0.4.0 quantreg_5.54 - ## [37] prettyunits_1.1.1 tools_3.6.2 rsvd_1.0.2 - ## [40] igraph_1.2.5 coda_0.19-3 gtable_0.3.0 - ## [43] glue_1.3.1 RANN_2.6.1 reshape2_1.4.3 - ## [46] dplyr_0.8.3 rappdirs_0.3.1 Rcpp_1.0.5 - ## [49] spatstat_1.64-1 scattermore_0.7 vctrs_0.2.1 - ## [52] nlme_3.1-142 lmtest_0.9-38 xfun_0.11 - ## [55] stringr_1.4.0 globals_0.13.0 mime_0.8 - ## [58] miniUI_0.1.1.1 lifecycle_0.1.0 irlba_2.3.3 - ## [61] goftest_1.2-2 future_1.18.0 MASS_7.3-51.4 - ## [64] zoo_1.8-7 scales_1.1.0 hms_0.5.2 - ## [67] promises_1.1.0 spatstat.utils_1.17-0 parallel_3.6.2 - ## [70] SparseM_1.78 RColorBrewer_1.1-2 yaml_2.2.0 - ## [73] reticulate_1.16 pbapply_1.4-3 gridExtra_2.3 - ## [76] ggplot2_3.3.0 rpart_4.1-15 stringi_1.4.3 - ## [79] rlang_0.4.5 pkgconfig_2.0.3 matrixStats_0.56.0 - ## [82] evaluate_0.14 lattice_0.20-38 ROCR_1.0-11 - ## [85] purrr_0.3.3 tensor_1.5 patchwork_1.0.1 - ## [88] htmlwidgets_1.5.1 cowplot_1.0.0 tidyselect_0.2.5 - ## [91] RcppAnnoy_0.0.18 plyr_1.8.5 magrittr_1.5 - ## [94] R6_2.4.1 pillar_1.4.3 mgcv_1.8-31 - ## [97] fitdistrplus_1.1-1 survival_3.1-8 abind_1.4-5 - ## [100] tibble_2.1.3 future.apply_1.6.0 crayon_1.3.4 - ## [103] KernSmooth_2.23-16 plotly_4.9.2 rmarkdown_2.3 - ## [106] progress_1.2.2 grid_3.6.2 data.table_1.12.8 - ## [109] digest_0.6.23 xtable_1.8-4 tidyr_1.0.0 - ## [112] httpuv_1.5.2 MCMCpack_1.4-5 RcppParallel_4.4.2 - ## [115] munsell_0.5.0 viridisLite_0.3.0 quadprog_1.5-8 From ccccf1b28d24c3ddac28d6985cd61b4da6867dd9 Mon Sep 17 00:00:00 2001 From: Peter Carbonetto Date: Fri, 15 Jan 2021 16:34:26 -0600 Subject: [PATCH 12/50] Added fastTopics to README. --- README.md | 1 + docs/fasttopics.md | 371 +++++++-------------------------------------- 2 files changed, 52 insertions(+), 320 deletions(-) diff --git a/README.md b/README.md index 4862f24..920d408 100644 --- a/README.md +++ b/README.md @@ -29,3 +29,4 @@ remotes::install_github('satijalab/seurat-wrappers') | schex | [Using schex with Seurat](http://htmlpreview.github.io/?https://github.com/satijalab/seurat-wrappers/blob/master/docs/schex.html) | Freytag, R package 2019 | https://github.com/SaskiaFreytag/schex | | alevin | [Import alevin counts into Seurat](http://htmlpreview.github.io/?https://github.com/satijalab/seurat-wrappers/blob/master/docs/alevin.html) | Srivastava et. al., Genome Biology 2019 | https://github.com/k3yavi/alevin-Rtools | | Nebulosa | [Visualization of gene expression with Nebulosa](http://htmlpreview.github.io/?https://github.com/satijalab/seurat-wrappers/blob/master/docs/nebulosa.html) | Jose Alquicira-Hernandez and Joseph E. Powell, _Under Review_ | https://github.com/powellgenomicslab/Nebulosa | +| fastTopics | [](http://htmlpreview.github.io/?https://github.com/stephenslab/seurat-wrappers/blob/master/docs/fasttopics.html) | Peter Carbonetto and Matthew Stephens, _Under Review_ | https://github.com/stephenslab/fastTopics | diff --git a/docs/fasttopics.md b/docs/fasttopics.md index d2aef84..207bf22 100644 --- a/docs/fasttopics.md +++ b/docs/fasttopics.md @@ -2,11 +2,26 @@ Analyzing Seurat data using fastTopics ================ Compiled: January 15, 2021 -*Add introductory text here.* - -*Point to the fastTopics website and vignettes.* - -*Add citation here.* +Here we illustrate the use of the +[fastTopics](https://github.com/stephenslab/fastTopics) Seurat wrapper +to analyze a Seurat data set. This vignette is only intended to +introduce the basic fastTopics interface for Seurat objects—for +background and practical guidance on analysis of single-cell RNA-seq +data using a topic model, please see the [fastTopics +vignettes](https://stephenslab.github.io/fastTopics/articles). + +If you find the **fastTopics** package useful for your work, please +cite: + +Kushal K. Dey, Chiaowen Joyce Hsiao and Matthew Stephens (2017). +[Visualizing the structure of RNA-seq expression data using grade of +membership models.](https://doi.org/10.1371/journal.pgen.1006599) *PLoS +Genetics* **13**, e1006599. + +Peter Carbonetto, Kevin Luo, Kushal Dey, Joyce Hsiao and Matthew +Stephens (2021). fastTopics: fast algorithms for fitting topic models +and non-negative matrix factorizations to count data. R package version +0.4-23. We begin by loading the packages used to perform the analysis. @@ -15,6 +30,7 @@ library(Seurat) library(SeuratData) library(SeuratWrappers) library(fastTopics) +library(cowplot) ``` We set the seed so that the results can be reproduced. @@ -47,333 +63,48 @@ we have chosen \(K = 6\) topics. In most settings, a good choice of results from topic models at different settings of \(K\). This plot shows the cells projected onto the top two principal -components (PCs) obtained from the topic mixture proportions. +components (PCs) of the topic mixture proportions. ``` r Idents(pbmc3k) <- pbmc3k$seurat_annotations -DimPlot(pbmc3k,reduction = "pca_topics",pt.size = 1) +DimPlot(pbmc3k,reduction = "pca_topics",pt.size = 1) + + theme_cowplot(font_size = 10) ``` -Compare this against PCs 1 and 2 of the transformed counts: +Compare this against the top two PCs of the transformed counts: ``` r pbmc3k <- FindVariableFeatures(pbmc3k) pbmc3k <- ScaleData(pbmc3k) -# Centering and scaling data matrix pbmc3k <- RunPCA(pbmc3k) -# PC_ 1 -# Positive: FTL, FTH1, COTL1, CST3, OAZ1, ACTB, LGALS1, S100A4, AIF1, FCER1G -# TMSB4X, S100A6, TYROBP, LST1, PSAP, TYMP, SAT1, S100A11, CTSS, SPI1 -# SERPINA1, LYZ, TMSB10, IFITM3, HLA-DRB1, FCN1, CFD, HLA-DPA1, VIM, GSTP1 -# Negative: MALAT1, IL32, LTB, CCL5, CTSW, CD247, CD2, NKG7, LINC00926, ACAP1 -# TCL1A, CST7, GZMA, FGFBP2, NCR3, BEX2, GZMK, HOPX, GNLY, MAL -# SAMD3, STK17A, MYC, XCL1, SPON2, NELL2, LDLRAP1, ZAP70, XCL2, PRF1 -# PC_ 2 -# Positive: FTL, TYROBP, S100A8, S100A9, FCN1, AIF1, FTH1, LYZ, CTSS, LST1 -# CFD, S100A6, TYMP, SAT1, SERPINA1, LGALS2, S100A11, PSAP, S100A4, CST3 -# FCER1G, IFITM3, CFP, APOBEC3A, LGALS1, SPI1, LGALS3, TIMP1, NPC2, IFI30 -# Negative: ACTG1, STMN1, TUBA1B, TYMS, ZWINT, GZMA, KIAA0101, TK1, RRM2, HMGB2 -# H2AFZ, DUT, HMGA1, FEN1, MYBL2, BIRC5, GINS2, GAPDH, SRSF3, NKG7 -# HSP90AA1, PTTG1, IL32, FABP5, ASF1B, MKI67, RANBP1, KIFC1, CENPM, ACTB -# PC_ 3 -# Positive: CD74, HLA-DRA, HLA-DPB1, HLA-DQB1, HLA-DQA1, HLA-DRB1, HLA-DPA1, HLA-DQA2, HLA-DRB5, LYZ -# HLA-DMB, CD1C, CST3, VIM, LGALS2, HLA-DMA, MS4A1, CD79A, CLEC10A, LINC00926 -# CD79B, GSTP1, MS4A6A, TCL1A, S100A9, FCER1A, PLD4, FCN1, S100A8, IFI6 -# Negative: PPBP, GNG11, SPARC, PF4, AP001189.4, SDPR, ITGA2B, CLU, CD9, TREML1 -# GP9, LY6G6F, CMTM5, NRGN, TUBB1, RP11-367G6.3, C6orf25, GP1BA, RGS18, F13A1 -# CA2, SCGB1C1, RUFY1, CLDN5, SEPT5, CLEC1B, ITGB3, HIST1H2AC, TUBA4A, CTTN -# PC_ 4 -# Positive: CD74, HLA-DQB1, HLA-DQA1, HLA-DRA, HLA-DQA2, HLA-DPB1, CD79A, HLA-DRB1, MS4A1, HLA-DPA1 -# HLA-DRB5, CD79B, HLA-DMB, CD1C, LINC00926, GPX1, TCL1A, HLA-DMA, KIAA0125, PPBP -# CLEC10A, P2RX5, PPP1R14A, PF4, PLD4, SPARC, FCER1A, GNG11, ITM2C, ITGA2B -# Negative: NKG7, GZMA, GNLY, PRF1, FGFBP2, SPON2, CTSW, FCGR3A, CST7, GZMB -# AKR1C3, CLIC3, GZMH, CCL5, CD247, XCL2, HOPX, IFITM2, CCL4, XCL1 -# FCER1G, TTC38, S100A4, CTSC, IL32, LST1, MS4A7, SERPINA1, AIF1, SAMD3 -# PC_ 5 -# Positive: ZWINT, KIAA0101, RRM2, HMGB2, AQP3, FEN1, TYMS, H2AFZ, GINS2, KIFC1 -# TK1, MYBL2, HN1, BIRC5, LTB, S100A8, FTL, NUSAP1, CDC45, CKS1B -# DUT, CENPM, MKI67, STMN1, CDCA5, ASF1B, S100A9, GAPDH, CFD, AIF1 -# Negative: NKG7, CD74, HLA-DQA1, GNLY, SPON2, HLA-DPB1, FGFBP2, HLA-DQA2, HLA-DRA, GZMB -# HLA-DQB1, PRF1, HLA-DPA1, HLA-DRB1, GZMA, CTSW, HLA-DRB5, CLIC3, AKR1C3, CST7 -# CD1C, XCL2, CCL4, XCL1, HOPX, CCL5, CLEC10A, HLA-DMB, TTC38, FCER1A -DimPlot(pbmc3k,reduction = "pca",pt.size = 1) +DimPlot(pbmc3k,reduction = "pca",pt.size = 1) + + theme_cowplot(font_size = 10) ``` -Once fitted topic model is extracted, many functions from the fastTopics -package can be used for analysis and visualization. For example, the -Structure plot provides an evocative visual summary of the estimated -mixture proportions for each cell. +The fitted topic model—a “multinom\_topic\_model” object—is stored in +the “misc” slot: ``` r fit <- Misc(Reductions(pbmc3k,"multinom_topic_model")) +``` + +Once the fitted topic model is extracted, many functions from the +**fastTopics** package can be used for analysis and visualization. For +example, the Structure plot provides an evocative visual summary of the +estimated mixture proportions for each cell. Here, we have grouped the +cells by previously inferred +labels. + +``` r structure_plot(fit,grouping = Idents(pbmc3k),gap = 25) -# Perplexity automatically changed to 83 because original setting of 100 was too large for the number of samples (255) -# Perplexity automatically changed to 65 because original setting of 100 was too large for the number of samples (201) -# Perplexity automatically changed to 38 because original setting of 100 was too large for the number of samples (120) -# Perplexity automatically changed to 37 because original setting of 100 was too large for the number of samples (115) -# Perplexity automatically changed to 6 because original setting of 100 was too large for the number of samples (24) -# Perplexity automatically changed to 2 because original setting of 100 was too large for the number of samples (10) ``` - # Read the 516 x 6 data matrix successfully! - # OpenMP is working. 1 threads. - # Using no_dims = 1, perplexity = 100.000000, and theta = 0.100000 - # Computing input similarities... - # Building tree... - # Done in 0.15 seconds (sparsity = 0.725084)! - # Learning embedding... - # Iteration 50: error is 46.544798 (50 iterations in 0.07 seconds) - # Iteration 100: error is 44.191974 (50 iterations in 0.06 seconds) - # Iteration 150: error is 44.191098 (50 iterations in 0.06 seconds) - # Iteration 200: error is 44.191097 (50 iterations in 0.06 seconds) - # Iteration 250: error is 44.191097 (50 iterations in 0.05 seconds) - # Iteration 300: error is 0.267504 (50 iterations in 0.06 seconds) - # Iteration 350: error is 0.265744 (50 iterations in 0.06 seconds) - # Iteration 400: error is 0.265741 (50 iterations in 0.06 seconds) - # Iteration 450: error is 0.265742 (50 iterations in 0.06 seconds) - # Iteration 500: error is 0.265742 (50 iterations in 0.06 seconds) - # Iteration 550: error is 0.265742 (50 iterations in 0.06 seconds) - # Iteration 600: error is 0.265742 (50 iterations in 0.05 seconds) - # Iteration 650: error is 0.265742 (50 iterations in 0.06 seconds) - # Iteration 700: error is 0.265742 (50 iterations in 0.06 seconds) - # Iteration 750: error is 0.265742 (50 iterations in 0.06 seconds) - # Iteration 800: error is 0.265742 (50 iterations in 0.06 seconds) - # Iteration 850: error is 0.265742 (50 iterations in 0.06 seconds) - # Iteration 900: error is 0.265742 (50 iterations in 0.06 seconds) - # Iteration 950: error is 0.265742 (50 iterations in 0.06 seconds) - # Iteration 1000: error is 0.265742 (50 iterations in 0.06 seconds) - # Fitting performed in 1.14 seconds. - # Read the 358 x 6 data matrix successfully! - # OpenMP is working. 1 threads. - # Using no_dims = 1, perplexity = 100.000000, and theta = 0.100000 - # Computing input similarities... - # Building tree... - # Done in 0.10 seconds (sparsity = 0.948269)! - # Learning embedding... - # Iteration 50: error is 42.965050 (50 iterations in 0.04 seconds) - # Iteration 100: error is 41.889224 (50 iterations in 0.04 seconds) - # Iteration 150: error is 41.888879 (50 iterations in 0.04 seconds) - # Iteration 200: error is 41.888918 (50 iterations in 0.04 seconds) - # Iteration 250: error is 41.888924 (50 iterations in 0.04 seconds) - # Iteration 300: error is 0.154191 (50 iterations in 0.04 seconds) - # Iteration 350: error is 0.154156 (50 iterations in 0.04 seconds) - # Iteration 400: error is 0.154155 (50 iterations in 0.04 seconds) - # Iteration 450: error is 0.154155 (50 iterations in 0.04 seconds) - # Iteration 500: error is 0.154155 (50 iterations in 0.04 seconds) - # Iteration 550: error is 0.154155 (50 iterations in 0.04 seconds) - # Iteration 600: error is 0.154155 (50 iterations in 0.04 seconds) - # Iteration 650: error is 0.154155 (50 iterations in 0.04 seconds) - # Iteration 700: error is 0.154155 (50 iterations in 0.04 seconds) - # Iteration 750: error is 0.154155 (50 iterations in 0.04 seconds) - # Iteration 800: error is 0.154155 (50 iterations in 0.04 seconds) - # Iteration 850: error is 0.154155 (50 iterations in 0.04 seconds) - # Iteration 900: error is 0.154155 (50 iterations in 0.04 seconds) - # Iteration 950: error is 0.154155 (50 iterations in 0.04 seconds) - # Iteration 1000: error is 0.154155 (50 iterations in 0.04 seconds) - # Fitting performed in 0.73 seconds. - # Read the 356 x 6 data matrix successfully! - # OpenMP is working. 1 threads. - # Using no_dims = 1, perplexity = 100.000000, and theta = 0.100000 - # Computing input similarities... - # Building tree... - # Done in 0.10 seconds (sparsity = 0.948886)! - # Learning embedding... - # Iteration 50: error is 44.127036 (50 iterations in 0.04 seconds) - # Iteration 100: error is 42.960180 (50 iterations in 0.04 seconds) - # Iteration 150: error is 42.946318 (50 iterations in 0.03 seconds) - # Iteration 200: error is 42.946371 (50 iterations in 0.04 seconds) - # Iteration 250: error is 42.946352 (50 iterations in 0.04 seconds) - # Iteration 300: error is 0.264561 (50 iterations in 0.04 seconds) - # Iteration 350: error is 0.264515 (50 iterations in 0.03 seconds) - # Iteration 400: error is 0.264520 (50 iterations in 0.04 seconds) - # Iteration 450: error is 0.264520 (50 iterations in 0.03 seconds) - # Iteration 500: error is 0.264520 (50 iterations in 0.03 seconds) - # Iteration 550: error is 0.264520 (50 iterations in 0.04 seconds) - # Iteration 600: error is 0.264520 (50 iterations in 0.04 seconds) - # Iteration 650: error is 0.264520 (50 iterations in 0.03 seconds) - # Iteration 700: error is 0.264520 (50 iterations in 0.04 seconds) - # Iteration 750: error is 0.264520 (50 iterations in 0.03 seconds) - # Iteration 800: error is 0.264520 (50 iterations in 0.03 seconds) - # Iteration 850: error is 0.264520 (50 iterations in 0.03 seconds) - # Iteration 900: error is 0.264520 (50 iterations in 0.03 seconds) - # Iteration 950: error is 0.264520 (50 iterations in 0.03 seconds) - # Iteration 1000: error is 0.264520 (50 iterations in 0.03 seconds) - # Fitting performed in 0.71 seconds. - # Read the 255 x 6 data matrix successfully! - # OpenMP is working. 1 threads. - # Using no_dims = 1, perplexity = 83.000000, and theta = 0.100000 - # Computing input similarities... - # Building tree... - # Done in 0.05 seconds (sparsity = 0.995309)! - # Learning embedding... - # Iteration 50: error is 41.894862 (50 iterations in 0.03 seconds) - # Iteration 100: error is 41.348727 (50 iterations in 0.02 seconds) - # Iteration 150: error is 41.316669 (50 iterations in 0.02 seconds) - # Iteration 200: error is 41.331596 (50 iterations in 0.02 seconds) - # Iteration 250: error is 41.337796 (50 iterations in 0.02 seconds) - # Iteration 300: error is 0.153763 (50 iterations in 0.02 seconds) - # Iteration 350: error is 0.152951 (50 iterations in 0.02 seconds) - # Iteration 400: error is 0.152945 (50 iterations in 0.02 seconds) - # Iteration 450: error is 0.152945 (50 iterations in 0.02 seconds) - # Iteration 500: error is 0.152945 (50 iterations in 0.02 seconds) - # Iteration 550: error is 0.152945 (50 iterations in 0.02 seconds) - # Iteration 600: error is 0.152945 (50 iterations in 0.02 seconds) - # Iteration 650: error is 0.152947 (50 iterations in 0.02 seconds) - # Iteration 700: error is 0.152948 (50 iterations in 0.02 seconds) - # Iteration 750: error is 0.152947 (50 iterations in 0.02 seconds) - # Iteration 800: error is 0.152945 (50 iterations in 0.02 seconds) - # Iteration 850: error is 0.152945 (50 iterations in 0.02 seconds) - # Iteration 900: error is 0.152947 (50 iterations in 0.02 seconds) - # Iteration 950: error is 0.152945 (50 iterations in 0.02 seconds) - # Iteration 1000: error is 0.152947 (50 iterations in 0.02 seconds) - # Fitting performed in 0.46 seconds. - # Read the 201 x 6 data matrix successfully! - # OpenMP is working. 1 threads. - # Using no_dims = 1, perplexity = 65.000000, and theta = 0.100000 - # Computing input similarities... - # Building tree... - # Done in 0.03 seconds (sparsity = 0.993688)! - # Learning embedding... - # Iteration 50: error is 43.455428 (50 iterations in 0.02 seconds) - # Iteration 100: error is 42.659459 (50 iterations in 0.02 seconds) - # Iteration 150: error is 42.729750 (50 iterations in 0.02 seconds) - # Iteration 200: error is 43.218125 (50 iterations in 0.02 seconds) - # Iteration 250: error is 43.736809 (50 iterations in 0.02 seconds) - # Iteration 300: error is 0.664267 (50 iterations in 0.02 seconds) - # Iteration 350: error is 0.649046 (50 iterations in 0.02 seconds) - # Iteration 400: error is 0.581421 (50 iterations in 0.02 seconds) - # Iteration 450: error is 0.581122 (50 iterations in 0.02 seconds) - # Iteration 500: error is 0.581124 (50 iterations in 0.02 seconds) - # Iteration 550: error is 0.581123 (50 iterations in 0.02 seconds) - # Iteration 600: error is 0.581120 (50 iterations in 0.02 seconds) - # Iteration 650: error is 0.581120 (50 iterations in 0.02 seconds) - # Iteration 700: error is 0.581120 (50 iterations in 0.02 seconds) - # Iteration 750: error is 0.581124 (50 iterations in 0.02 seconds) - # Iteration 800: error is 0.581124 (50 iterations in 0.02 seconds) - # Iteration 850: error is 0.581123 (50 iterations in 0.02 seconds) - # Iteration 900: error is 0.581124 (50 iterations in 0.02 seconds) - # Iteration 950: error is 0.581120 (50 iterations in 0.02 seconds) - # Iteration 1000: error is 0.581120 (50 iterations in 0.02 seconds) - # Fitting performed in 0.35 seconds. - # Read the 120 x 6 data matrix successfully! - # OpenMP is working. 1 threads. - # Using no_dims = 1, perplexity = 38.000000, and theta = 0.100000 - # Computing input similarities... - # Building tree... - # Done in 0.01 seconds (sparsity = 0.988750)! - # Learning embedding... - # Iteration 50: error is 45.952359 (50 iterations in 0.01 seconds) - # Iteration 100: error is 48.966439 (50 iterations in 0.01 seconds) - # Iteration 150: error is 48.365154 (50 iterations in 0.01 seconds) - # Iteration 200: error is 50.364318 (50 iterations in 0.01 seconds) - # Iteration 250: error is 50.683345 (50 iterations in 0.01 seconds) - # Iteration 300: error is 1.136895 (50 iterations in 0.01 seconds) - # Iteration 350: error is 0.733483 (50 iterations in 0.01 seconds) - # Iteration 400: error is 0.625430 (50 iterations in 0.01 seconds) - # Iteration 450: error is 0.625001 (50 iterations in 0.01 seconds) - # Iteration 500: error is 0.624999 (50 iterations in 0.01 seconds) - # Iteration 550: error is 0.624999 (50 iterations in 0.01 seconds) - # Iteration 600: error is 0.624999 (50 iterations in 0.01 seconds) - # Iteration 650: error is 0.624986 (50 iterations in 0.01 seconds) - # Iteration 700: error is 0.624986 (50 iterations in 0.01 seconds) - # Iteration 750: error is 0.625000 (50 iterations in 0.01 seconds) - # Iteration 800: error is 0.624986 (50 iterations in 0.01 seconds) - # Iteration 850: error is 0.624986 (50 iterations in 0.01 seconds) - # Iteration 900: error is 0.625000 (50 iterations in 0.01 seconds) - # Iteration 950: error is 0.625000 (50 iterations in 0.01 seconds) - # Iteration 1000: error is 0.624999 (50 iterations in 0.01 seconds) - # Fitting performed in 0.17 seconds. - # Read the 115 x 6 data matrix successfully! - # OpenMP is working. 1 threads. - # Using no_dims = 1, perplexity = 37.000000, and theta = 0.100000 - # Computing input similarities... - # Building tree... - # Done in 0.01 seconds (sparsity = 0.989792)! - # Learning embedding... - # Iteration 50: error is 49.065562 (50 iterations in 0.01 seconds) - # Iteration 100: error is 49.210910 (50 iterations in 0.01 seconds) - # Iteration 150: error is 49.974686 (50 iterations in 0.01 seconds) - # Iteration 200: error is 51.228638 (50 iterations in 0.01 seconds) - # Iteration 250: error is 49.220168 (50 iterations in 0.01 seconds) - # Iteration 300: error is 1.749707 (50 iterations in 0.01 seconds) - # Iteration 350: error is 1.094096 (50 iterations in 0.01 seconds) - # Iteration 400: error is 0.868036 (50 iterations in 0.01 seconds) - # Iteration 450: error is 0.845715 (50 iterations in 0.01 seconds) - # Iteration 500: error is 0.845736 (50 iterations in 0.01 seconds) - # Iteration 550: error is 0.845737 (50 iterations in 0.01 seconds) - # Iteration 600: error is 0.845737 (50 iterations in 0.01 seconds) - # Iteration 650: error is 0.845737 (50 iterations in 0.01 seconds) - # Iteration 700: error is 0.845737 (50 iterations in 0.01 seconds) - # Iteration 750: error is 0.845736 (50 iterations in 0.01 seconds) - # Iteration 800: error is 0.845736 (50 iterations in 0.01 seconds) - # Iteration 850: error is 0.845737 (50 iterations in 0.01 seconds) - # Iteration 900: error is 0.845737 (50 iterations in 0.01 seconds) - # Iteration 950: error is 0.845737 (50 iterations in 0.01 seconds) - # Iteration 1000: error is 0.845736 (50 iterations in 0.01 seconds) - # Fitting performed in 0.16 seconds. - # Read the 24 x 6 data matrix successfully! - # OpenMP is working. 1 threads. - # Using no_dims = 1, perplexity = 6.000000, and theta = 0.100000 - # Computing input similarities... - # Building tree... - # Done in 0.00 seconds (sparsity = 0.881944)! - # Learning embedding... - # Iteration 50: error is 70.976567 (50 iterations in 0.00 seconds) - # Iteration 100: error is 72.024095 (50 iterations in 0.00 seconds) - # Iteration 150: error is 72.360917 (50 iterations in 0.00 seconds) - # Iteration 200: error is 71.631815 (50 iterations in 0.00 seconds) - # Iteration 250: error is 73.191667 (50 iterations in 0.00 seconds) - # Iteration 300: error is 6.587625 (50 iterations in 0.00 seconds) - # Iteration 350: error is 3.246346 (50 iterations in 0.00 seconds) - # Iteration 400: error is 2.966947 (50 iterations in 0.00 seconds) - # Iteration 450: error is 2.960004 (50 iterations in 0.00 seconds) - # Iteration 500: error is 2.959519 (50 iterations in 0.00 seconds) - # Iteration 550: error is 2.959505 (50 iterations in 0.00 seconds) - # Iteration 600: error is 2.959505 (50 iterations in 0.00 seconds) - # Iteration 650: error is 2.959505 (50 iterations in 0.00 seconds) - # Iteration 700: error is 2.959505 (50 iterations in 0.00 seconds) - # Iteration 750: error is 2.959504 (50 iterations in 0.00 seconds) - # Iteration 800: error is 2.959503 (50 iterations in 0.00 seconds) - # Iteration 850: error is 2.959503 (50 iterations in 0.00 seconds) - # Iteration 900: error is 2.959502 (50 iterations in 0.00 seconds) - # Iteration 950: error is 2.959501 (50 iterations in 0.00 seconds) - # Iteration 1000: error is 2.959500 (50 iterations in 0.00 seconds) - # Fitting performed in 0.02 seconds. - # Read the 10 x 6 data matrix successfully! - # OpenMP is working. 1 threads. - # Using no_dims = 1, perplexity = 2.000000, and theta = 0.100000 - # Computing input similarities... - # Building tree... - # Done in 0.00 seconds (sparsity = 0.760000)! - # Learning embedding... - # Iteration 50: error is 91.006955 (50 iterations in 0.00 seconds) - # Iteration 100: error is 69.804871 (50 iterations in 0.00 seconds) - # Iteration 150: error is 82.678065 (50 iterations in 0.00 seconds) - # Iteration 200: error is 82.559507 (50 iterations in 0.00 seconds) - # Iteration 250: error is 130.669903 (50 iterations in 0.00 seconds) - # Iteration 300: error is 2.313263 (50 iterations in 0.00 seconds) - # Iteration 350: error is 2.268197 (50 iterations in 0.00 seconds) - # Iteration 400: error is 2.263634 (50 iterations in 0.00 seconds) - # Iteration 450: error is 2.262503 (50 iterations in 0.00 seconds) - # Iteration 500: error is 2.262447 (50 iterations in 0.00 seconds) - # Iteration 550: error is 2.262446 (50 iterations in 0.00 seconds) - # Iteration 600: error is 2.262446 (50 iterations in 0.00 seconds) - # Iteration 650: error is 2.262446 (50 iterations in 0.00 seconds) - # Iteration 700: error is 2.262446 (50 iterations in 0.00 seconds) - # Iteration 750: error is 2.262446 (50 iterations in 0.00 seconds) - # Iteration 800: error is 2.262446 (50 iterations in 0.00 seconds) - # Iteration 850: error is 2.262446 (50 iterations in 0.00 seconds) - # Iteration 900: error is 2.262446 (50 iterations in 0.00 seconds) - # Iteration 950: error is 2.262446 (50 iterations in 0.00 seconds) - # Iteration 1000: error is 2.262446 (50 iterations in 0.00 seconds) - # Fitting performed in 0.00 seconds. - This is the version of R and the packages that were used to generate these results. @@ -394,8 +125,8 @@ sessionInfo() # [1] stats graphics grDevices utils datasets methods base # # other attached packages: -# [1] fastTopics_0.4-23 SeuratWrappers_0.3.2 pbmc3k.SeuratData_3.1.4 -# [4] SeuratData_0.2.1 Seurat_3.2.3 +# [1] cowplot_1.0.0 fastTopics_0.4-23 SeuratWrappers_0.3.2 +# [4] pbmc3k.SeuratData_3.1.4 SeuratData_0.2.1 Seurat_3.2.3 # # loaded via a namespace (and not attached): # [1] Rtsne_0.15 colorspace_1.4-1 deldir_0.1-29 @@ -428,14 +159,14 @@ sessionInfo() # [82] matrixStats_0.56.0 evaluate_0.14 lattice_0.20-38 # [85] ROCR_1.0-11 purrr_0.3.3 tensor_1.5 # [88] labeling_0.3 patchwork_1.0.1 htmlwidgets_1.5.1 -# [91] cowplot_1.0.0 tidyselect_0.2.5 RcppAnnoy_0.0.18 -# [94] plyr_1.8.5 magrittr_1.5 R6_2.4.1 -# [97] pillar_1.4.3 mgcv_1.8-31 fitdistrplus_1.1-1 -# [100] survival_3.1-8 abind_1.4-5 tibble_2.1.3 -# [103] future.apply_1.6.0 crayon_1.3.4 KernSmooth_2.23-16 -# [106] plotly_4.9.2 rmarkdown_2.3 progress_1.2.2 -# [109] grid_3.6.2 data.table_1.12.8 digest_0.6.23 -# [112] xtable_1.8-4 tidyr_1.0.0 httpuv_1.5.2 -# [115] MCMCpack_1.4-5 RcppParallel_4.4.2 munsell_0.5.0 -# [118] viridisLite_0.3.0 quadprog_1.5-8 +# [91] tidyselect_0.2.5 RcppAnnoy_0.0.18 plyr_1.8.5 +# [94] magrittr_1.5 R6_2.4.1 pillar_1.4.3 +# [97] mgcv_1.8-31 fitdistrplus_1.1-1 survival_3.1-8 +# [100] abind_1.4-5 tibble_2.1.3 future.apply_1.6.0 +# [103] crayon_1.3.4 KernSmooth_2.23-16 plotly_4.9.2 +# [106] rmarkdown_2.3 progress_1.2.2 grid_3.6.2 +# [109] data.table_1.12.8 digest_0.6.23 xtable_1.8-4 +# [112] tidyr_1.0.0 httpuv_1.5.2 MCMCpack_1.4-5 +# [115] RcppParallel_4.4.2 munsell_0.5.0 viridisLite_0.3.0 +# [118] quadprog_1.5-8 ``` From 023a5db0ec5c818f63f665867ccb40833e26eeac Mon Sep 17 00:00:00 2001 From: Peter Carbonetto Date: Fri, 15 Jan 2021 16:36:18 -0600 Subject: [PATCH 13/50] Added vignette title in README. --- README.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/README.md b/README.md index 920d408..e360a0e 100644 --- a/README.md +++ b/README.md @@ -29,4 +29,4 @@ remotes::install_github('satijalab/seurat-wrappers') | schex | [Using schex with Seurat](http://htmlpreview.github.io/?https://github.com/satijalab/seurat-wrappers/blob/master/docs/schex.html) | Freytag, R package 2019 | https://github.com/SaskiaFreytag/schex | | alevin | [Import alevin counts into Seurat](http://htmlpreview.github.io/?https://github.com/satijalab/seurat-wrappers/blob/master/docs/alevin.html) | Srivastava et. al., Genome Biology 2019 | https://github.com/k3yavi/alevin-Rtools | | Nebulosa | [Visualization of gene expression with Nebulosa](http://htmlpreview.github.io/?https://github.com/satijalab/seurat-wrappers/blob/master/docs/nebulosa.html) | Jose Alquicira-Hernandez and Joseph E. Powell, _Under Review_ | https://github.com/powellgenomicslab/Nebulosa | -| fastTopics | [](http://htmlpreview.github.io/?https://github.com/stephenslab/seurat-wrappers/blob/master/docs/fasttopics.html) | Peter Carbonetto and Matthew Stephens, _Under Review_ | https://github.com/stephenslab/fastTopics | +| fastTopics | [Analyzing Seurat data using fastTopics](http://htmlpreview.github.io/?https://github.com/stephenslab/seurat-wrappers/blob/master/docs/fasttopics.html) | Peter Carbonetto and Matthew Stephens, _Under Review_ | https://github.com/stephenslab/fastTopics | From 3988ce48c15adb6bf0ac4a0c2cf1e32468f4feb4 Mon Sep 17 00:00:00 2001 From: Peter Carbonetto Date: Fri, 15 Jan 2021 16:41:16 -0600 Subject: [PATCH 14/50] A couple of small edits to the fasttopics code. --- R/fasttopics.R | 2 -- docs/fasttopics.Rmd | 4 ++-- 2 files changed, 2 insertions(+), 4 deletions(-) diff --git a/R/fasttopics.R b/R/fasttopics.R index f9a6250..72a55c3 100644 --- a/R/fasttopics.R +++ b/R/fasttopics.R @@ -1,5 +1,3 @@ -# TO DO: Implement FitPoissonNMF. - #' @title Fit a Multinomial Topic Model Using fastTopics #' #' @description Fits a multinomial topic model to the raw count data, diff --git a/docs/fasttopics.Rmd b/docs/fasttopics.Rmd index 746e679..b650f0d 100644 --- a/docs/fasttopics.Rmd +++ b/docs/fasttopics.Rmd @@ -13,8 +13,8 @@ output: Here we illustrate the use of the [fastTopics][fasttopics] Seurat wrapper to analyze a Seurat data set. This vignette is only intended to introduce the basic fastTopics interface for Seurat objects---for -background and practical guidance on analysis of single-cell RNA-seq -data using a topic model, please see the +background and guidance on analysis of single-cell RNA-seq data using +a topic model, please see the [fastTopics vignettes][fasttopics-vignettes]. If you find the **fastTopics** package useful for your work, please From 080fd0e28036c2cd393d2fc0e0b826f26aa1f67a Mon Sep 17 00:00:00 2001 From: Peter Carbonetto Date: Fri, 15 Jan 2021 21:12:11 -0600 Subject: [PATCH 15/50] Fixed PCA plot in fasttopics vignette. --- docs/fasttopics.Rmd | 1 + docs/fasttopics.html | 5 +++-- docs/fasttopics.md | 5 +++-- 3 files changed, 7 insertions(+), 4 deletions(-) diff --git a/docs/fasttopics.Rmd b/docs/fasttopics.Rmd index b650f0d..77b6dd5 100644 --- a/docs/fasttopics.Rmd +++ b/docs/fasttopics.Rmd @@ -85,6 +85,7 @@ Compare this against the top two PCs of the transformed counts: ```{r pca-2, fig.width=4.5, fig.height=3, message=FALSE} pbmc3k <- FindVariableFeatures(pbmc3k) +pbmc3k <- NormalizeData(pbmc3k) pbmc3k <- ScaleData(pbmc3k) pbmc3k <- RunPCA(pbmc3k) DimPlot(pbmc3k,reduction = "pca",pt.size = 1) + diff --git a/docs/fasttopics.html b/docs/fasttopics.html index 2b19dd3..1576e9a 100644 --- a/docs/fasttopics.html +++ b/docs/fasttopics.html @@ -377,7 +377,7 @@

Compiled: January 15, 2021

-

Here we illustrate the use of the fastTopics Seurat wrapper to analyze a Seurat data set. This vignette is only intended to introduce the basic fastTopics interface for Seurat objects—for background and practical guidance on analysis of single-cell RNA-seq data using a topic model, please see the fastTopics vignettes.

+

Here we illustrate the use of the fastTopics Seurat wrapper to analyze a Seurat data set. This vignette is only intended to introduce the basic fastTopics interface for Seurat objects—for background and guidance on analysis of single-cell RNA-seq data using a topic model, please see the fastTopics vignettes.

If you find the fastTopics package useful for your work, please cite:

Kushal K. Dey, Chiaowen Joyce Hsiao and Matthew Stephens (2017). Visualizing the structure of RNA-seq expression data using grade of membership models. PLoS Genetics 13, e1006599.

Peter Carbonetto, Kevin Luo, Kushal Dey, Joyce Hsiao and Matthew Stephens (2021). fastTopics: fast algorithms for fitting topic models and non-negative matrix factorizations to count data. R package version 0.4-23. https://github.com/stephenslab/fastTopics

@@ -404,11 +404,12 @@

Compiled: January 15, 2021

Compare this against the top two PCs of the transformed counts:

pbmc3k <- FindVariableFeatures(pbmc3k)
+pbmc3k <- NormalizeData(pbmc3k)
 pbmc3k <- ScaleData(pbmc3k)
 pbmc3k <- RunPCA(pbmc3k)
 DimPlot(pbmc3k,reduction = "pca",pt.size = 1) +
   theme_cowplot(font_size = 10)
-

+

The fitted topic model—a “multinom_topic_model” object—is stored in the “misc” slot:

fit <- Misc(Reductions(pbmc3k,"multinom_topic_model"))

Once the fitted topic model is extracted, many functions from the fastTopics package can be used for analysis and visualization. For example, the Structure plot provides an evocative visual summary of the estimated mixture proportions for each cell. Here, we have grouped the cells by previously inferred labels.

diff --git a/docs/fasttopics.md b/docs/fasttopics.md index 207bf22..81d9343 100644 --- a/docs/fasttopics.md +++ b/docs/fasttopics.md @@ -6,8 +6,8 @@ Here we illustrate the use of the [fastTopics](https://github.com/stephenslab/fastTopics) Seurat wrapper to analyze a Seurat data set. This vignette is only intended to introduce the basic fastTopics interface for Seurat objects—for -background and practical guidance on analysis of single-cell RNA-seq -data using a topic model, please see the [fastTopics +background and guidance on analysis of single-cell RNA-seq data using a +topic model, please see the [fastTopics vignettes](https://stephenslab.github.io/fastTopics/articles). If you find the **fastTopics** package useful for your work, please @@ -77,6 +77,7 @@ Compare this against the top two PCs of the transformed counts: ``` r pbmc3k <- FindVariableFeatures(pbmc3k) +pbmc3k <- NormalizeData(pbmc3k) pbmc3k <- ScaleData(pbmc3k) pbmc3k <- RunPCA(pbmc3k) DimPlot(pbmc3k,reduction = "pca",pt.size = 1) + From 0482d57332518b34993d5157e575626c422085cd Mon Sep 17 00:00:00 2001 From: Peter Carbonetto Date: Sun, 17 Jan 2021 21:03:35 -0600 Subject: [PATCH 16/50] Implemented helper functions prepare_counts_fasttopics and pca_from_loadings_fasttopics. --- NAMESPACE | 1 + R/fasttopics.R | 138 +++++++++++++++++++++++++++++++++++-------- docs/fasttopics.Rmd | 2 +- man/FitPoissonNMF.Rd | 77 ++++++++++++++++++++++++ man/FitTopicModel.Rd | 7 ++- 5 files changed, 196 insertions(+), 29 deletions(-) create mode 100644 man/FitPoissonNMF.Rd diff --git a/NAMESPACE b/NAMESPACE index 2cb4f67..a17d831 100644 --- a/NAMESPACE +++ b/NAMESPACE @@ -7,6 +7,7 @@ S3method(as.Seurat,cell_data_set) S3method(as.Seurat,list) S3method(as.cell_data_set,Seurat) export(ALRAChooseKPlot) +export(FitPoissonNMF) export(FitTopicModel) export(ReadAlevin) export(ReadVelocity) diff --git a/R/fasttopics.R b/R/fasttopics.R index 72a55c3..a7947e4 100644 --- a/R/fasttopics.R +++ b/R/fasttopics.R @@ -1,3 +1,75 @@ +#' @title Add Title Here. +#' +#' @description Add description here. +#' +#' @param object A Seurat object. +#' +#' @param k An integer 2 or greater giving the matrix rank. This +#' argument should only be specified if the initial fit (\code{fit0}) +#' is not provided. +#' +#' @param numiter +#' +#' @param assay Name of assay to use; defaults to the default +#' assay of the object. +#' +#' @details Add details here. +#' +#' @param reduction.name Name of the outputted reduction. +#' +#' @param reduction.key Key for the outputted reduction. +#' +#' @param verbose When \code{verbose = TRUE}, information about the +#' progress of the model fitting is printed to the console. See +#' \code{\link[fastTopics]{fit_poisson_nmf}} for an explanation of the +#' output. +#' +#' @param \dots Additional arguments passed to \code{fit_poisson_nmf}; +#' see \code{\link[fastTopics]{fit_poisson_nmf}} for details. +#' +#' @return Describe the return value here. +#' +#' @author Peter Carbonetto +#' +#' @references +#' Lee, D. D. and Seung, H. S. (2001). Algorithms for non-negative +#' matrix factorization. In \emph{Advances in Neural Information +#' Processing Systems} \bold{13}, 556–562. +#' +#' @seealso \code{\link{FitTopicModel}}, +#' \code{\link[fastTopics]{fit_poisson_nmf}} +#' +#' @examples +#' library(Seurat) +#' library(fastTopics) +#' set.seed(1) +#' data(pbmc_small) +#' +#' # Fit the non-negative matrix factorization to the raw UMI count +#' # data; no pre-processing or pre-selection of genes is needed. +#' pbmc_small <- FitPoissonNMF(pbmc_small,k = 3) +#' +#' @export +#' +FitPoissonNMF <- function (object, k, assay = NULL, features = NULL, + reduction.name = "poisson_nmf", + reduction.key = "k_", numiter = 100, + method = c("scd", "em", "mu", "ccd"), + init.method = c("topicscore", "random"), + control = list(), verbose = TRUE, ...) { + + # Check the input arguments, and that fastTopics is installed. + CheckPackage(package = "stephenslab/fastTopics") + if (!inherits(object,"Seurat")) + stop("\"object\" must be a Seurat object",call. = FALSE) + + # TO DO: Check if Seurat object contains a "poisson_nmf" reduction. + + # Get the n x m counts matrix, where n is the number of samples + # (cells) and m is the number of selected features. + # TO DO. +} + #' @title Fit a Multinomial Topic Model Using fastTopics #' #' @description Fits a multinomial topic model to the raw count data, @@ -31,9 +103,9 @@ #' assay of the object. #' #' @param features A list of features to use for fitting the model. If -#' \code{features = NULL}, \emph{all} features are used; in -#' particular, ; see \code{\link[Seurat]{VariableFeatures}} is \emph{not -#' used to pre-select features. +#' \code{features = NULL}, \emph{all} features are used; in +#' particular, \code{\link[Seurat]{VariableFeatures}} is \emph{not} +#' used to pre-select features. #' #' @param reduction.name Name of the outputted reduction. #' @@ -90,8 +162,6 @@ #' library(Seurat) #' library(fastTopics) #' set.seed(1) -#' -#' # Load the PBMC data. #' data(pbmc_small) #' #' # Fit the multinomial topic model to the raw UMI count data; no @@ -130,21 +200,11 @@ FitTopicModel <- function (object, k = 3, assay = NULL, features = NULL, stop("\"object\" must be a Seurat object",call. = FALSE) # Get the n x m counts matrix, where n is the number of samples - # (cells) and m is the number of selected genes. + # (cells) and m is the number of selected features. assay <- assay %||% DefaultAssay(object) DefaultAssay(object) <- assay - X <- GetAssayData(object,"counts") - if (is.null(features)) - features <- rownames(X) - else - features <- intersect(features,rownames(X)) - X <- X[features,] - X <- t(X) - - # Remove all-zero columns. - i <- which(colSums(X > 0) >= 1) - features <- features[i] - X <- X[,i] + X <- prepare_counts_fasttopics(object,features) + features <- colnames(X) # Fit the multinomial topic model using fastTopics. fit <- fit_topic_model(X,k,verbose = verbose,...) @@ -158,18 +218,44 @@ FitTopicModel <- function (object, k = 3, assay = NULL, features = NULL, # Add the topic model fit to the Seurat object. object[[reduction.name]] <- - CreateDimReducObject(embeddings,loadings,assay = assay,key = reduction.key, - global = TRUE,misc = fit) + CreateDimReducObject(embeddings,loadings,assay = assay, + key = reduction.key,global = TRUE, + misc = fit) # Add a PCA dimension reduction calculated from the mixture # proportions. - out <- prcomp(fit$L) - colnames(out$x) <- paste0("TOPICPC_",1:k) - colnames(out$rotation) <- paste0("TOPICPC_",1:k) - object[["pca_topics"]] <- - CreateDimReducObject(out$x[,-k],out$rotation[,-k],assay = assay, - key = "TOPICPC_",global = TRUE) + object[["pca_topics"]] <- pca_from_loadings_fasttopics(fit,assay,"TOPICPC_") # Output the updated Seurat object. return(LogSeuratCommand(object)) } + +# Get the n x m counts matrix, where n is the number of samples +# (cells) and m is the number of selected features (columns). An +# additional step is taken to remove all-zero columns. +prepare_counts_fasttopics <- function (object, features) { + X <- GetAssayData(object,"counts") + if (is.null(features)) + features <- rownames(X) + else + features <- intersect(features,rownames(X)) + X <- X[features,] + X <- t(X) + + # Remove all-zero columns. + i <- which(colSums(X > 0) >= 1) + return(X[,i]) +} + +# Generate a Seurat PCA dimension reduction object from the loadings +# matrix. +pca_from_loadings_fasttopics <- function (fit, assay, reduction.key, + min.sdev = 1e-8) { + k <- ncol(fit$L) + out <- prcomp(fit$L) + colnames(out$x) <- paste0(reduction.key,1:k) + colnames(out$rotation) <- paste0(reduction.key,1:k) + cols <- which(out$sdev > min.sdev) + return(CreateDimReducObject(out$x[,cols],out$rotation[,cols],assay = assay, + key = reduction.key,global = TRUE)) +} diff --git a/docs/fasttopics.Rmd b/docs/fasttopics.Rmd index 77b6dd5..7bcfa01 100644 --- a/docs/fasttopics.Rmd +++ b/docs/fasttopics.Rmd @@ -63,7 +63,7 @@ Fit the multinomial topic model to the raw UMI counts---*no pre-processing or pre-selection of genes is needed.* Note that it may take several minutes to complete this model fitting step. -```{r fit-model, results="hide", message=FALSE, cache=TRUE} +```{r fit-model, results="hide", message=FALSE} pbmc3k <- FitTopicModel(pbmc3k,k = 6) ``` diff --git a/man/FitPoissonNMF.Rd b/man/FitPoissonNMF.Rd new file mode 100644 index 0000000..cdab48a --- /dev/null +++ b/man/FitPoissonNMF.Rd @@ -0,0 +1,77 @@ +% Generated by roxygen2: do not edit by hand +% Please edit documentation in R/fasttopics.R +\name{FitPoissonNMF} +\alias{FitPoissonNMF} +\title{Add Title Here.} +\usage{ +FitPoissonNMF( + object, + k, + assay = NULL, + features = NULL, + reduction.name = "poisson_nmf", + reduction.key = "k_", + numiter = 100, + method = c("scd", "em", "mu", "ccd"), + init.method = c("topicscore", "random"), + control = list(), + verbose = TRUE, + ... +) +} +\arguments{ +\item{object}{A Seurat object.} + +\item{k}{An integer 2 or greater giving the matrix rank. This +argument should only be specified if the initial fit (\code{fit0}) +is not provided.} + +\item{assay}{Name of assay to use; defaults to the default +assay of the object.} + +\item{reduction.name}{Name of the outputted reduction.} + +\item{reduction.key}{Key for the outputted reduction.} + +\item{numiter}{} + +\item{verbose}{When \code{verbose = TRUE}, information about the +progress of the model fitting is printed to the console. See +\code{\link[fastTopics]{fit_poisson_nmf}} for an explanation of the +output.} + +\item{\dots}{Additional arguments passed to \code{fit_poisson_nmf}; +see \code{\link[fastTopics]{fit_poisson_nmf}} for details.} +} +\value{ +Describe the return value here. +} +\description{ +Add description here. +} +\details{ +Add details here. +} +\examples{ +library(Seurat) +library(fastTopics) +set.seed(1) +data(pbmc_small) + +# Fit the non-negative matrix factorization to the raw UMI count +# data; no pre-processing or pre-selection of genes is needed. +pbmc_small <- FitPoissonNMF(pbmc_small,k = 3) + +} +\references{ +Lee, D. D. and Seung, H. S. (2001). Algorithms for non-negative + matrix factorization. In \emph{Advances in Neural Information + Processing Systems} \bold{13}, 556–562. +} +\seealso{ +\code{\link{FitTopicModel}}, + \code{\link[fastTopics]{fit_poisson_nmf}} +} +\author{ +Peter Carbonetto +} diff --git a/man/FitTopicModel.Rd b/man/FitTopicModel.Rd index 3b78e1c..0d9c3cd 100644 --- a/man/FitTopicModel.Rd +++ b/man/FitTopicModel.Rd @@ -23,6 +23,11 @@ FitTopicModel( \item{assay}{Name of assay to use; defaults to the default assay of the object.} +\item{features}{A list of features to use for fitting the model. If +\code{features = NULL}, \emph{all} features are used; in +particular, \code{\link[Seurat]{VariableFeatures}} is \emph{not} +used to pre-select features.} + \item{reduction.name}{Name of the outputted reduction.} \item{reduction.key}{Key for the outputted reduction.} @@ -94,8 +99,6 @@ See \code{\link[fastTopics]{fit_topic_model}} for more details. library(Seurat) library(fastTopics) set.seed(1) - -# Load the PBMC data. data(pbmc_small) # Fit the multinomial topic model to the raw UMI count data; no From dba83082851de3cfce8829a6777d7f1a77f8627b Mon Sep 17 00:00:00 2001 From: Peter Carbonetto Date: Sun, 17 Jan 2021 21:37:40 -0600 Subject: [PATCH 17/50] Implemented FitPoissonNMF. --- NAMESPACE | 1 + R/fasttopics.R | 69 +++++++++++++++++++++++++++++++++++++++----- man/FitPoissonNMF.Rd | 16 +++++++++- 3 files changed, 77 insertions(+), 9 deletions(-) diff --git a/NAMESPACE b/NAMESPACE index a17d831..054489f 100644 --- a/NAMESPACE +++ b/NAMESPACE @@ -58,6 +58,7 @@ importFrom(Seurat,as.Graph) importFrom(Seurat,as.Seurat) importFrom(Seurat,as.SingleCellExperiment) importFrom(cowplot,theme_cowplot) +importFrom(fastTopics,fit_poisson_nmf) importFrom(fastTopics,fit_topic_model) importFrom(ggplot2,aes_string) importFrom(ggplot2,geom_line) diff --git a/R/fasttopics.R b/R/fasttopics.R index a7947e4..60dad01 100644 --- a/R/fasttopics.R +++ b/R/fasttopics.R @@ -47,7 +47,24 @@ #' #' # Fit the non-negative matrix factorization to the raw UMI count #' # data; no pre-processing or pre-selection of genes is needed. -#' pbmc_small <- FitPoissonNMF(pbmc_small,k = 3) +#' pbmc_small <- FitPoissonNMF(pbmc_small,k = 3,numiter = 20) +#' +#' # Improve the fit by running another 20 updates. +#' pbmc_small <- FitPoissonNMF(pbmc_small,k = 3,numiter = 20) + +#' # This plot shows the cells projected onto the 2 principal +#' # components (PCs) of the topic mixture proportions. +#' DimPlot(pbmc_small,reduction = "pca_nmf") +#' +#' # Compare this against the top two PCs of the transformed count +#' # data. +#' DimPlot(pbmc_small,reduction = "pca") +#' +#' # Extract the non-negative matrix factorization. +#' fit <- Misc(Reductions(pbmc_small,"poisson_nmf")) +#' summary(fit) +#' +#' @importFrom fastTopics fit_poisson_nmf #' #' @export #' @@ -62,12 +79,46 @@ FitPoissonNMF <- function (object, k, assay = NULL, features = NULL, CheckPackage(package = "stephenslab/fastTopics") if (!inherits(object,"Seurat")) stop("\"object\" must be a Seurat object",call. = FALSE) - - # TO DO: Check if Seurat object contains a "poisson_nmf" reduction. - + # Get the n x m counts matrix, where n is the number of samples # (cells) and m is the number of selected features. - # TO DO. + assay <- assay %||% DefaultAssay(object) + DefaultAssay(object) <- assay + X <- prepare_counts_fasttopics(object,features) + features <- colnames(X) + + # Fit the Poisson non-negative matrix factorization using + # fastTopics. If Seurat object has an existing "poisson_nmf" + # reduction, use this to initialize the model fitting. + if (is.element("poisson_nmf",Reductions(object))) { + fit0 <- Misc(Reductions(object,"poisson_nmf")) + fit <- fit_poisson_nmf(X,fit0 = fit0,numiter = numiter,method = method, + init.method = init.method,control = control, + verbose = verbose,...) + } else + fit <- fit_poisson_nmf(X,k,numiter = numiter,method = method, + init.method = init.method,control = control, + verbose = verbose,...) + class(fit) <- c("list","poisson_nmf_fit") + + # Retrieve the factors matrix (n x k) and loadings matrix (m x k). + embeddings <- fit$L + loadings <- fit$F + colnames(embeddings) <- paste0(reduction.key,1:k) + colnames(loadings) <- paste0(reduction.key,1:k) + + # Add the topic model fit to the Seurat object. + object[[reduction.name]] <- + CreateDimReducObject(embeddings,loadings,assay = assay, + key = reduction.key,global = TRUE, + misc = fit) + + # Add a PCA dimension reduction calculated from the mixture + # proportions. + object[["pca_nmf"]] <- pca_from_loadings_fasttopics(fit,assay,"NMFPC_") + + # Output the updated Seurat object. + return(LogSeuratCommand(object)) } #' @title Fit a Multinomial Topic Model Using fastTopics @@ -183,9 +234,6 @@ FitPoissonNMF <- function (object, k, assay = NULL, features = NULL, #' fit <- Misc(Reductions(pbmc_small,"multinom_topic_model")) #' structure_plot(fit,grouping = Idents(pbmc_small),gap = 5) #' -#' @importFrom stats prcomp -#' @importFrom Matrix colSums -#' @importFrom Matrix t #' @importFrom fastTopics fit_topic_model #' #' @export @@ -233,6 +281,9 @@ FitTopicModel <- function (object, k = 3, assay = NULL, features = NULL, # Get the n x m counts matrix, where n is the number of samples # (cells) and m is the number of selected features (columns). An # additional step is taken to remove all-zero columns. +# +#' @importFrom Matrix colSums +#' @importFrom Matrix t prepare_counts_fasttopics <- function (object, features) { X <- GetAssayData(object,"counts") if (is.null(features)) @@ -249,6 +300,8 @@ prepare_counts_fasttopics <- function (object, features) { # Generate a Seurat PCA dimension reduction object from the loadings # matrix. +# +#' @importFrom stats prcomp pca_from_loadings_fasttopics <- function (fit, assay, reduction.key, min.sdev = 1e-8) { k <- ncol(fit$L) diff --git a/man/FitPoissonNMF.Rd b/man/FitPoissonNMF.Rd index cdab48a..b682aa5 100644 --- a/man/FitPoissonNMF.Rd +++ b/man/FitPoissonNMF.Rd @@ -60,7 +60,21 @@ data(pbmc_small) # Fit the non-negative matrix factorization to the raw UMI count # data; no pre-processing or pre-selection of genes is needed. -pbmc_small <- FitPoissonNMF(pbmc_small,k = 3) +pbmc_small <- FitPoissonNMF(pbmc_small,k = 3,numiter = 20) + +# Improve the fit by running another 20 updates. +pbmc_small <- FitPoissonNMF(pbmc_small,k = 3,numiter = 20) +# This plot shows the cells projected onto the 2 principal +# components (PCs) of the topic mixture proportions. +DimPlot(pbmc_small,reduction = "pca_nmf") + +# Compare this against the top two PCs of the transformed count +# data. +DimPlot(pbmc_small,reduction = "pca") + +# Extract the non-negative matrix factorization. +fit <- Misc(Reductions(pbmc_small,"poisson_nmf")) +summary(fit) } \references{ From a61ad9505ef57e98aaa02cae66a011692f544867 Mon Sep 17 00:00:00 2001 From: Peter Carbonetto Date: Mon, 18 Jan 2021 22:06:37 -0600 Subject: [PATCH 18/50] Filled out roxygen2 docs for FitPoissonNMF. --- R/fasttopics.R | 87 ++++++++++++++++++++++++++++++++------------ man/FitPoissonNMF.Rd | 60 +++++++++++++++++++++++++----- man/FitTopicModel.Rd | 21 ++++++----- 3 files changed, 125 insertions(+), 43 deletions(-) diff --git a/R/fasttopics.R b/R/fasttopics.R index 60dad01..fa1d4bd 100644 --- a/R/fasttopics.R +++ b/R/fasttopics.R @@ -1,33 +1,73 @@ -#' @title Add Title Here. -#' -#' @description Add description here. -#' -#' @param object A Seurat object. +#' @title Fit a Non-negative Matrix Factorization Using fastTopics +#' +#' @description Approximate the raw count data \code{X} by the +#' non-negative matrix factorization \code{tcrossprod(L,F)}, in which +#' the quality of the approximation is measured by a +#' \dQuote{divergence} criterion; equivalently, optimize the +#' likelihood under a Poisson model of the count data, \code{X}, in +#' which the Poisson rates are given by \code{tcrossprod(L,F)}. +#' +#' @param object A Seurat object. If this Seurat object contains a +#' Poisson NMF dimensionality reduction object (\dQuote{DimReduc}), +#' the model fitting will be initialized to this previously fitted +#' Poisson NMF. #' #' @param k An integer 2 or greater giving the matrix rank. This #' argument should only be specified if the initial fit (\code{fit0}) #' is not provided. #' -#' @param numiter -#' #' @param assay Name of assay to use; defaults to the default #' assay of the object. #' -#' @details Add details here. +#' @param features A list of features to use for fitting the model. If +#' \code{features = NULL}, \emph{all} features are used; in +#' particular, \code{\link[Seurat]{VariableFeatures}} is \emph{not} +#' used to pre-select features. #' #' @param reduction.name Name of the outputted reduction. #' #' @param reduction.key Key for the outputted reduction. #' +#' @param numiter The number of updates of the factors and loadings to +#' perform. +#' +#' @param method The method to use for updating the factors and +#' loadings. See \code{\link[fastTopics]{fit_poisson_nmf}} for +#' details. +#' +#' @param init.method The method used to initialize the factors and +#' loadings. See \code{\link[fastTopics]{fit_poisson_nmf}} for +#' details. +#' +#' @param control A list of parameters controlling the behaviour of +#' the optimization algorithm. See +#' \code{\link[fastTopics]{fit_poisson_nmf}} for details. +#' #' @param verbose When \code{verbose = TRUE}, information about the #' progress of the model fitting is printed to the console. See #' \code{\link[fastTopics]{fit_poisson_nmf}} for an explanation of the #' output. #' -#' @param \dots Additional arguments passed to \code{fit_poisson_nmf}; -#' see \code{\link[fastTopics]{fit_poisson_nmf}} for details. +#' @param \dots Additional arguments passed to \code{fit_poisson_nmf}. +#' +#' @return A Seurat object, in which the Poisson NMF fit is stored as +#' a Seurat \code{\link[Seurat]{DimReduc}} object. The cell embeddings +#' (stored in the \code{cell.embeddings} slot) are the loadings; this +#' is the n x k matrix \code{L} outputted by \code{fit_poisson_nmf} (n +#' is the number of cells and k is the dimension of the matrix +#' factorization). +#' +#' The feature loadings (stored in the \code{feature.loadings} slot) +#' are the m x k factors matrix \code{F} outputted by +#' \code{fit_poisson_nmf}, where m is the number of features. #' -#' @return Describe the return value here. +#' Apply \code{\link[Seurat]{Misc}} to the \code{DimReduce} object to +#' access the \dQuote{"poisson_nmf_fit"} object outputted by +#' \code{\link[fastTopics]{fit_poisson_nmf}}; see the example for an +#' illustration. +#' +#' An additional PCA dimension reduction computed from the k loadings +#' is provided. #' #' @author Peter Carbonetto #' @@ -51,7 +91,7 @@ #' #' # Improve the fit by running another 20 updates. #' pbmc_small <- FitPoissonNMF(pbmc_small,k = 3,numiter = 20) - +#' #' # This plot shows the cells projected onto the 2 principal #' # components (PCs) of the topic mixture proportions. #' DimPlot(pbmc_small,reduction = "pca_nmf") @@ -120,7 +160,7 @@ FitPoissonNMF <- function (object, k, assay = NULL, features = NULL, # Output the updated Seurat object. return(LogSeuratCommand(object)) } - + #' @title Fit a Multinomial Topic Model Using fastTopics #' #' @description Fits a multinomial topic model to the raw count data, @@ -178,7 +218,8 @@ FitPoissonNMF <- function (object, k, assay = NULL, features = NULL, #' number of topics). #' #' The feature loadings (stored in the \code{feature.loadings} slot) -#' are an m x k matrix of relative expression levels; this is the +#' are an m x k matrix of relative expression levels, where m is the +#' number of features and k is the number of topics; this is the #' matrix \code{F} outputted by \code{fit_topic_model}. #' #' Apply \code{\link[Seurat]{Misc}} to the \code{DimReduce} object to @@ -189,15 +230,15 @@ FitPoissonNMF <- function (object, k, assay = NULL, features = NULL, #' \code{\link[fastTopics]{fit_topic_model}} more information about #' the "multinom_topic_model_fit" object. #' -#' An additional PCA dimension reduction on \code{k-1} dimensions is -#' provided. This could be useful, for example, to quickly visualize -#' the cells using \code{\link[Seurat]{DimPlot}}. The principal -#' components are computed from the topic mixture -#' proportions. However, this reduction is provided for convenience -#' only, and we recommend to extract the -#' \dQuote{"multinom_topic_model_fit"} object and use the dedicated -#' visualization tools that are provided in the fastTopics package -#' such as \code{\link[fastTopics]{structure_plot}}. +#' An additional PCA dimension reduction on \code{k-1} dimensions, +#' computed from the mixture proportions, is provided. This could be +#' useful, for example, to quickly visualize the cells using +#' \code{\link[Seurat]{DimPlot}}. The principal components are +#' computed from the topic mixture proportions. However, this +#' reduction is provided for convenience only, and we recommend to +#' extract the \dQuote{"multinom_topic_model_fit"} object and use the +#' dedicated visualization tools that are provided in the fastTopics +#' package such as \code{\link[fastTopics]{structure_plot}}. #' #' @author Peter Carbonetto #' diff --git a/man/FitPoissonNMF.Rd b/man/FitPoissonNMF.Rd index b682aa5..5d3c245 100644 --- a/man/FitPoissonNMF.Rd +++ b/man/FitPoissonNMF.Rd @@ -2,7 +2,7 @@ % Please edit documentation in R/fasttopics.R \name{FitPoissonNMF} \alias{FitPoissonNMF} -\title{Add Title Here.} +\title{Fit a Non-negative Matrix Factorization Using fastTopics} \usage{ FitPoissonNMF( object, @@ -20,7 +20,10 @@ FitPoissonNMF( ) } \arguments{ -\item{object}{A Seurat object.} +\item{object}{A Seurat object. If this Seurat object contains a +Poisson NMF dimensionality reduction object (\dQuote{DimReduc}), +the model fitting will be initialized to this previously fitted +Poisson NMF.} \item{k}{An integer 2 or greater giving the matrix rank. This argument should only be specified if the initial fit (\code{fit0}) @@ -29,28 +32,64 @@ is not provided.} \item{assay}{Name of assay to use; defaults to the default assay of the object.} +\item{features}{A list of features to use for fitting the model. If +\code{features = NULL}, \emph{all} features are used; in +particular, \code{\link[Seurat]{VariableFeatures}} is \emph{not} +used to pre-select features.} + \item{reduction.name}{Name of the outputted reduction.} \item{reduction.key}{Key for the outputted reduction.} -\item{numiter}{} +\item{numiter}{The number of updates of the factors and loadings to +perform.} + +\item{method}{The method to use for updating the factors and +loadings. See \code{\link[fastTopics]{fit_poisson_nmf}} for +details.} + +\item{init.method}{The method used to initialize the factors and +loadings. See \code{\link[fastTopics]{fit_poisson_nmf}} for +details.} + +\item{control}{A list of parameters controlling the behaviour of +the optimization algorithm. See +\code{\link[fastTopics]{fit_poisson_nmf}} for details.} \item{verbose}{When \code{verbose = TRUE}, information about the progress of the model fitting is printed to the console. See \code{\link[fastTopics]{fit_poisson_nmf}} for an explanation of the output.} -\item{\dots}{Additional arguments passed to \code{fit_poisson_nmf}; -see \code{\link[fastTopics]{fit_poisson_nmf}} for details.} +\item{\dots}{Additional arguments passed to \code{fit_poisson_nmf}.} } \value{ -Describe the return value here. +A Seurat object, in which the Poisson NMF fit is stored as +a Seurat \code{\link[Seurat]{DimReduc}} object. The cell embeddings +(stored in the \code{cell.embeddings} slot) are the loadings; this +is the n x k matrix \code{L} outputted by \code{fit_poisson_nmf} (n +is the number of cells and k is the dimension of the matrix +factorization). + +The feature loadings (stored in the \code{feature.loadings} slot) +are the m x k factors matrix \code{F} outputted by +\code{fit_poisson_nmf}, where m is the number of features. + +Apply \code{\link[Seurat]{Misc}} to the \code{DimReduce} object to +access the \dQuote{"poisson_nmf_fit"} object outputted by +\code{\link[fastTopics]{fit_poisson_nmf}}; see the example for an +illustration. + +An additional PCA dimension reduction computed from the k loadings +is provided. } \description{ -Add description here. -} -\details{ -Add details here. +Approximate the raw count data \code{X} by the + non-negative matrix factorization \code{tcrossprod(L,F)}, in which + the quality of the approximation is measured by a + \dQuote{divergence} criterion; equivalently, optimize the + likelihood under a Poisson model of the count data, \code{X}, in + which the Poisson rates are given by \code{tcrossprod(L,F)}. } \examples{ library(Seurat) @@ -64,6 +103,7 @@ pbmc_small <- FitPoissonNMF(pbmc_small,k = 3,numiter = 20) # Improve the fit by running another 20 updates. pbmc_small <- FitPoissonNMF(pbmc_small,k = 3,numiter = 20) + # This plot shows the cells projected onto the 2 principal # components (PCs) of the topic mixture proportions. DimPlot(pbmc_small,reduction = "pca_nmf") diff --git a/man/FitTopicModel.Rd b/man/FitTopicModel.Rd index 0d9c3cd..6a44746 100644 --- a/man/FitTopicModel.Rd +++ b/man/FitTopicModel.Rd @@ -49,7 +49,8 @@ mixture proportions; this is the n x k matrix \code{L} outputted by number of topics). The feature loadings (stored in the \code{feature.loadings} slot) -are an m x k matrix of relative expression levels; this is the +are an m x k matrix of relative expression levels, where m is the +number of features and k is the number of topics; this is the matrix \code{F} outputted by \code{fit_topic_model}. Apply \code{\link[Seurat]{Misc}} to the \code{DimReduce} object to @@ -60,15 +61,15 @@ here for an illustration of how to access the model fit, and see \code{\link[fastTopics]{fit_topic_model}} more information about the "multinom_topic_model_fit" object. -An additional PCA dimension reduction on \code{k-1} dimensions is -provided. This could be useful, for example, to quickly visualize -the cells using \code{\link[Seurat]{DimPlot}}. The principal -components are computed from the topic mixture -proportions. However, this reduction is provided for convenience -only, and we recommend to extract the -\dQuote{"multinom_topic_model_fit"} object and use the dedicated -visualization tools that are provided in the fastTopics package -such as \code{\link[fastTopics]{structure_plot}}. +An additional PCA dimension reduction on \code{k-1} dimensions, +computed from the mixture proportions, is provided. This could be +useful, for example, to quickly visualize the cells using +\code{\link[Seurat]{DimPlot}}. The principal components are +computed from the topic mixture proportions. However, this +reduction is provided for convenience only, and we recommend to +extract the \dQuote{"multinom_topic_model_fit"} object and use the +dedicated visualization tools that are provided in the fastTopics +package such as \code{\link[fastTopics]{structure_plot}}. } \description{ Fits a multinomial topic model to the raw count data, From c604eb4fb0020a4c1246e9e4959f1b696db507ee Mon Sep 17 00:00:00 2001 From: Peter Carbonetto Date: Fri, 19 Feb 2021 07:15:43 -0600 Subject: [PATCH 19/50] Added notes from Alan. --- .Rbuildignore | 2 ++ NOTES.txt | 29 +++++++++++++++++++++++++++++ R/fasttopics.R | 2 +- 3 files changed, 32 insertions(+), 1 deletion(-) create mode 100644 NOTES.txt diff --git a/.Rbuildignore b/.Rbuildignore index 4952b99..5f06524 100644 --- a/.Rbuildignore +++ b/.Rbuildignore @@ -1,3 +1,5 @@ ^.*\.Rproj$ ^\.Rproj\.user$ ^docs$ +^NOTES\.txt$ + diff --git a/NOTES.txt b/NOTES.txt new file mode 100644 index 0000000..fe86d84 --- /dev/null +++ b/NOTES.txt @@ -0,0 +1,29 @@ +Comments from Alan Selewa: + +I was able to run the wrapper on my heart single cell RNA-seq +data. Theres about ~70k cells and 26k genes. Fitting the model took +about 4 hours using 16 Rcpp parallel threads. Heres some thoughts on +the process: + +FitTopicModel would benefit from more hyper-parameters in its +interface, like number of EM iterations to perform, number of parallel +threads to use, etc. Basically anything you’d pass to fastTopics +should be available in the interface, in my opinion. + +This is minor, but a progress bar would probably be more informative +to the average user, rather than printing the EM updates. For +debugging purposes, you can write the EM updates to a log file. + +I would add a function that works on the Seurat object to plot +log-likelihood vs iteration (similar to Seurat::ElbowPlot). + +It seems that downstream analyses (structure_plot, differential +expression) will work directly on the fit produced. In conformity with +everything else Seurat-related, I think these functions should work +directly on the Seurat object, rather than extracting the fit object, +and running functions found in fastTopics. + +The topic proportion of each cell should be visualized with +Seurat::FeaturePlots e.g. topic 1 proportion overlayed on a UMAP as I +shown in the vignette below add a wrapper function for visualizing +gene proportion in each topic. diff --git a/R/fasttopics.R b/R/fasttopics.R index fa1d4bd..22ae356 100644 --- a/R/fasttopics.R +++ b/R/fasttopics.R @@ -128,7 +128,7 @@ FitPoissonNMF <- function (object, k, assay = NULL, features = NULL, features <- colnames(X) # Fit the Poisson non-negative matrix factorization using - # fastTopics. If Seurat object has an existing "poisson_nmf" + # fastTopics. If Seurat object has an existing "poisson_nmf" # reduction, use this to initialize the model fitting. if (is.element("poisson_nmf",Reductions(object))) { fit0 <- Misc(Reductions(object,"poisson_nmf")) From c665ccbd108481f4de3ce76a1c01a5505e7a9c77 Mon Sep 17 00:00:00 2001 From: Peter Carbonetto Date: Thu, 25 Feb 2021 12:44:01 -0600 Subject: [PATCH 20/50] Added notes from Gilad Green. --- NOTES.txt | 8 ++++++++ 1 file changed, 8 insertions(+) diff --git a/NOTES.txt b/NOTES.txt index fe86d84..22ed570 100644 --- a/NOTES.txt +++ b/NOTES.txt @@ -27,3 +27,11 @@ The topic proportion of each cell should be visualized with Seurat::FeaturePlots e.g. topic 1 proportion overlayed on a UMAP as I shown in the vignette below add a wrapper function for visualizing gene proportion in each topic. + +Comments from Gilad Green: + +For the different functions of the wrapper (FitPoissonNMF and +FitTopicModel) I see you add two DimReduction objects. For the first +the user can specify `reduction.name` and `reduction.key` but for the +second the user cannot do so. Will it be possible to add those to the +function arguments too? From dd848d06fdd4075057e4d3d3027bd3804e18fbed Mon Sep 17 00:00:00 2001 From: Peter Carbonetto Date: Wed, 3 Mar 2021 16:58:40 -0600 Subject: [PATCH 21/50] Added reduction.pca.name and reduction.pca.key arguments to fastTopics interface. --- DESCRIPTION | 4 ++-- NOTES.txt | 12 ------------ R/fasttopics.R | 23 +++++++++++++++++++---- man/FitPoissonNMF.Rd | 6 ++++++ man/FitTopicModel.Rd | 6 ++++++ 5 files changed, 33 insertions(+), 18 deletions(-) diff --git a/DESCRIPTION b/DESCRIPTION index ea54492..8361eba 100644 --- a/DESCRIPTION +++ b/DESCRIPTION @@ -1,7 +1,7 @@ Package: SeuratWrappers Title: Community-Provided Methods and Extensions for the Seurat Object -Version: 0.3.2 -Date: 2021-01-14 +Version: 0.3.3 +Date: 2021-03-03 Authors@R: c( person(given = 'Rahul', family = 'Satija', email = 'rsatija@nygenome.org', role = 'aut', comment = c(ORCID = '0000-0001-9448-8833')), person(given = 'Andrew', family = 'Butler', email = 'abutler@nygenome.org', role = 'aut', comment = c(ORCID = '0000-0003-3608-0463')), diff --git a/NOTES.txt b/NOTES.txt index 22ed570..c866f2b 100644 --- a/NOTES.txt +++ b/NOTES.txt @@ -10,10 +10,6 @@ interface, like number of EM iterations to perform, number of parallel threads to use, etc. Basically anything you’d pass to fastTopics should be available in the interface, in my opinion. -This is minor, but a progress bar would probably be more informative -to the average user, rather than printing the EM updates. For -debugging purposes, you can write the EM updates to a log file. - I would add a function that works on the Seurat object to plot log-likelihood vs iteration (similar to Seurat::ElbowPlot). @@ -27,11 +23,3 @@ The topic proportion of each cell should be visualized with Seurat::FeaturePlots e.g. topic 1 proportion overlayed on a UMAP as I shown in the vignette below add a wrapper function for visualizing gene proportion in each topic. - -Comments from Gilad Green: - -For the different functions of the wrapper (FitPoissonNMF and -FitTopicModel) I see you add two DimReduction objects. For the first -the user can specify `reduction.name` and `reduction.key` but for the -second the user cannot do so. Will it be possible to add those to the -function arguments too? diff --git a/R/fasttopics.R b/R/fasttopics.R index 22ae356..fb5e57f 100644 --- a/R/fasttopics.R +++ b/R/fasttopics.R @@ -28,6 +28,10 @@ #' #' @param reduction.key Key for the outputted reduction. #' +#' @param reduction.pca.name Name of the outputted PCA reduction. +#' +#' @param reduction.pca.key Key for the outputted PCA reduction. +#' #' @param numiter The number of updates of the factors and loadings to #' perform. #' @@ -110,7 +114,9 @@ #' FitPoissonNMF <- function (object, k, assay = NULL, features = NULL, reduction.name = "poisson_nmf", - reduction.key = "k_", numiter = 100, + reduction.key = "k_", + reduction.pca.name = "pca_nmf", + reduction.pca.key = "NMFPC_", numiter = 100, method = c("scd", "em", "mu", "ccd"), init.method = c("topicscore", "random"), control = list(), verbose = TRUE, ...) { @@ -155,7 +161,8 @@ FitPoissonNMF <- function (object, k, assay = NULL, features = NULL, # Add a PCA dimension reduction calculated from the mixture # proportions. - object[["pca_nmf"]] <- pca_from_loadings_fasttopics(fit,assay,"NMFPC_") + object[[reduction.pca.name]] <- + pca_from_loadings_fasttopics(fit,assay,reduction.pca.key) # Output the updated Seurat object. return(LogSeuratCommand(object)) @@ -202,6 +209,10 @@ FitPoissonNMF <- function (object, k, assay = NULL, features = NULL, #' #' @param reduction.key Key for the outputted reduction. #' +#' @param reduction.pca.name Name of the outputted PCA reduction. +#' +#' @param reduction.pca.key Key for the outputted PCA reduction. +#' #' @param verbose When \code{verbose = TRUE}, information about the #' progress of the model fitting is printed to the console. See #' \code{\link[fastTopics]{fit_poisson_nmf}} for an explanation of the @@ -281,7 +292,10 @@ FitPoissonNMF <- function (object, k, assay = NULL, features = NULL, #' FitTopicModel <- function (object, k = 3, assay = NULL, features = NULL, reduction.name = "multinom_topic_model", - reduction.key = "k_", verbose = TRUE, ...) { + reduction.key = "k_", + reduction.pca.name = "pca_topics", + reduction.pca.key = "TOPICPC_", + verbose = TRUE, ...) { # Check the input arguments, and that fastTopics is installed. CheckPackage(package = "stephenslab/fastTopics") @@ -313,7 +327,8 @@ FitTopicModel <- function (object, k = 3, assay = NULL, features = NULL, # Add a PCA dimension reduction calculated from the mixture # proportions. - object[["pca_topics"]] <- pca_from_loadings_fasttopics(fit,assay,"TOPICPC_") + object[[reduction.pca.name]] <- + pca_from_loadings_fasttopics(fit,assay,reduction.pca.key) # Output the updated Seurat object. return(LogSeuratCommand(object)) diff --git a/man/FitPoissonNMF.Rd b/man/FitPoissonNMF.Rd index 5d3c245..c189066 100644 --- a/man/FitPoissonNMF.Rd +++ b/man/FitPoissonNMF.Rd @@ -11,6 +11,8 @@ FitPoissonNMF( features = NULL, reduction.name = "poisson_nmf", reduction.key = "k_", + reduction.pca.name = "pca_nmf", + reduction.pca.key = "NMFPC_", numiter = 100, method = c("scd", "em", "mu", "ccd"), init.method = c("topicscore", "random"), @@ -41,6 +43,10 @@ used to pre-select features.} \item{reduction.key}{Key for the outputted reduction.} +\item{reduction.pca.name}{Name of the outputted PCA reduction.} + +\item{reduction.pca.key}{Key for the outputted PCA reduction.} + \item{numiter}{The number of updates of the factors and loadings to perform.} diff --git a/man/FitTopicModel.Rd b/man/FitTopicModel.Rd index 6a44746..7312862 100644 --- a/man/FitTopicModel.Rd +++ b/man/FitTopicModel.Rd @@ -11,6 +11,8 @@ FitTopicModel( features = NULL, reduction.name = "multinom_topic_model", reduction.key = "k_", + reduction.pca.name = "pca_topics", + reduction.pca.key = "TOPICPC_", verbose = TRUE, ... ) @@ -32,6 +34,10 @@ used to pre-select features.} \item{reduction.key}{Key for the outputted reduction.} +\item{reduction.pca.name}{Name of the outputted PCA reduction.} + +\item{reduction.pca.key}{Key for the outputted PCA reduction.} + \item{verbose}{When \code{verbose = TRUE}, information about the progress of the model fitting is printed to the console. See \code{\link[fastTopics]{fit_poisson_nmf}} for an explanation of the From 5667953db9b0e077658de6460d9f303581231003 Mon Sep 17 00:00:00 2001 From: Peter Carbonetto Date: Wed, 3 Mar 2021 16:59:01 -0600 Subject: [PATCH 22/50] Added docs/fasttopics_files. --- docs/fasttopics_files/figure-gfm/pca-1-1.png | Bin 0 -> 59090 bytes docs/fasttopics_files/figure-gfm/pca-2-1.png | Bin 0 -> 69952 bytes .../figure-gfm/structure-plot-1.png | Bin 0 -> 45976 bytes docs/fasttopics_files/figure-html/pca-1-1.png | Bin 0 -> 129971 bytes docs/fasttopics_files/figure-html/pca-2-1.png | Bin 0 -> 149127 bytes .../figure-html/structure-plot-1.png | Bin 0 -> 101380 bytes 6 files changed, 0 insertions(+), 0 deletions(-) create mode 100644 docs/fasttopics_files/figure-gfm/pca-1-1.png create mode 100644 docs/fasttopics_files/figure-gfm/pca-2-1.png create mode 100644 docs/fasttopics_files/figure-gfm/structure-plot-1.png create mode 100644 docs/fasttopics_files/figure-html/pca-1-1.png create mode 100644 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z-KYEqNz-Vwu{P5GxKws%XnxMZ>~(+l`%#t8`TK{x+-2hQ99-k$bXYW|B??+!v8CM{$Gmhe?&eXl``6x78>zyjRo+>!pv%Mo{96G F{{Xonw&VZ+ literal 0 HcmV?d00001 From 1bad1f709a86f571e68eb4810f47af5323484c16 Mon Sep 17 00:00:00 2001 From: Peter Carbonetto Date: Wed, 3 Mar 2021 22:15:11 -0600 Subject: [PATCH 23/50] Updated verbose argument in FitTopicModel and FitPoissonNMF. --- DESCRIPTION | 2 +- R/fasttopics.R | 29 +++++++++++++++++++---------- man/FitPoissonNMF.Rd | 10 +++++----- man/FitTopicModel.Rd | 10 +++++----- 4 files changed, 30 insertions(+), 21 deletions(-) diff --git a/DESCRIPTION b/DESCRIPTION index 8361eba..f207daa 100644 --- a/DESCRIPTION +++ b/DESCRIPTION @@ -1,6 +1,6 @@ Package: SeuratWrappers Title: Community-Provided Methods and Extensions for the Seurat Object -Version: 0.3.3 +Version: 0.3.4 Date: 2021-03-03 Authors@R: c( person(given = 'Rahul', family = 'Satija', email = 'rsatija@nygenome.org', role = 'aut', comment = c(ORCID = '0000-0001-9448-8833')), diff --git a/R/fasttopics.R b/R/fasttopics.R index fb5e57f..97d1a5c 100644 --- a/R/fasttopics.R +++ b/R/fasttopics.R @@ -47,10 +47,10 @@ #' the optimization algorithm. See #' \code{\link[fastTopics]{fit_poisson_nmf}} for details. #' -#' @param verbose When \code{verbose = TRUE}, information about the -#' progress of the model fitting is printed to the console. See -#' \code{\link[fastTopics]{fit_poisson_nmf}} for an explanation of the -#' output. +#' @param verbose When \code{verbose = "progressbar"} or \code{verbose +#' = "detailed"}, information about the progress of the model fitting +#' is printed to the console. See \code{\link[fastTopics]{fit_poisson_nmf}} +#' for more information. #' #' @param \dots Additional arguments passed to \code{fit_poisson_nmf}. #' @@ -119,13 +119,18 @@ FitPoissonNMF <- function (object, k, assay = NULL, features = NULL, reduction.pca.key = "NMFPC_", numiter = 100, method = c("scd", "em", "mu", "ccd"), init.method = c("topicscore", "random"), - control = list(), verbose = TRUE, ...) { + control = list(), + verbose = c("progressbar", "detailed", "none"), + ...) { # Check the input arguments, and that fastTopics is installed. CheckPackage(package = "stephenslab/fastTopics") if (!inherits(object,"Seurat")) stop("\"object\" must be a Seurat object",call. = FALSE) + # Check and progress input argument "verbose". + verbose <- match.arg(verbose) + # Get the n x m counts matrix, where n is the number of samples # (cells) and m is the number of selected features. assay <- assay %||% DefaultAssay(object) @@ -213,10 +218,10 @@ FitPoissonNMF <- function (object, k, assay = NULL, features = NULL, #' #' @param reduction.pca.key Key for the outputted PCA reduction. #' -#' @param verbose When \code{verbose = TRUE}, information about the -#' progress of the model fitting is printed to the console. See -#' \code{\link[fastTopics]{fit_poisson_nmf}} for an explanation of the -#' output. +#' @param verbose When \code{verbose = "progressbar"} or \code{verbose +#' = "detailed"}, information about the progress of the model fitting +#' is printed to the console. See \code{\link[fastTopics]{fit_poisson_nmf}} +#' for more information. #' #' @param \dots Additional arguments passed to \code{fit_topic_model}; #' see \code{\link[fastTopics]{fit_topic_model}} for details. @@ -295,13 +300,17 @@ FitTopicModel <- function (object, k = 3, assay = NULL, features = NULL, reduction.key = "k_", reduction.pca.name = "pca_topics", reduction.pca.key = "TOPICPC_", - verbose = TRUE, ...) { + verbose = c("progressbar", "detailed", "none"), + ...) { # Check the input arguments, and that fastTopics is installed. CheckPackage(package = "stephenslab/fastTopics") if (!inherits(object,"Seurat")) stop("\"object\" must be a Seurat object",call. = FALSE) + # Check and progress input argument "verbose". + verbose <- match.arg(verbose) + # Get the n x m counts matrix, where n is the number of samples # (cells) and m is the number of selected features. assay <- assay %||% DefaultAssay(object) diff --git a/man/FitPoissonNMF.Rd b/man/FitPoissonNMF.Rd index c189066..8bc56b1 100644 --- a/man/FitPoissonNMF.Rd +++ b/man/FitPoissonNMF.Rd @@ -17,7 +17,7 @@ FitPoissonNMF( method = c("scd", "em", "mu", "ccd"), init.method = c("topicscore", "random"), control = list(), - verbose = TRUE, + verbose = c("progressbar", "detailed", "none"), ... ) } @@ -62,10 +62,10 @@ details.} the optimization algorithm. See \code{\link[fastTopics]{fit_poisson_nmf}} for details.} -\item{verbose}{When \code{verbose = TRUE}, information about the -progress of the model fitting is printed to the console. See -\code{\link[fastTopics]{fit_poisson_nmf}} for an explanation of the -output.} +\item{verbose}{When \code{verbose = "progressbar"} or \code{verbose += "detailed"}, information about the progress of the model fitting +is printed to the console. See \code{\link[fastTopics]{fit_poisson_nmf}} +for more information.} \item{\dots}{Additional arguments passed to \code{fit_poisson_nmf}.} } diff --git a/man/FitTopicModel.Rd b/man/FitTopicModel.Rd index 7312862..3d045fa 100644 --- a/man/FitTopicModel.Rd +++ b/man/FitTopicModel.Rd @@ -13,7 +13,7 @@ FitTopicModel( reduction.key = "k_", reduction.pca.name = "pca_topics", reduction.pca.key = "TOPICPC_", - verbose = TRUE, + verbose = c("progressbar", "detailed", "none"), ... ) } @@ -38,10 +38,10 @@ used to pre-select features.} \item{reduction.pca.key}{Key for the outputted PCA reduction.} -\item{verbose}{When \code{verbose = TRUE}, information about the -progress of the model fitting is printed to the console. See -\code{\link[fastTopics]{fit_poisson_nmf}} for an explanation of the -output.} +\item{verbose}{When \code{verbose = "progressbar"} or \code{verbose += "detailed"}, information about the progress of the model fitting +is printed to the console. See \code{\link[fastTopics]{fit_poisson_nmf}} +for more information.} \item{\dots}{Additional arguments passed to \code{fit_topic_model}; see \code{\link[fastTopics]{fit_topic_model}} for details.} From eefec42ca933472d419d98032265894201a9209b Mon Sep 17 00:00:00 2001 From: Peter Carbonetto Date: Wed, 15 Mar 2023 14:29:59 -0500 Subject: [PATCH 24/50] Removed .DS_Store. --- .DS_Store | Bin 6148 -> 0 bytes 1 file changed, 0 insertions(+), 0 deletions(-) delete mode 100644 .DS_Store diff --git a/.DS_Store b/.DS_Store deleted file mode 100644 index 8b9385a2e971887be8d8116f28d3e8d3bac77494..0000000000000000000000000000000000000000 GIT binary patch literal 0 HcmV?d00001 literal 6148 zcmeHK%}T>S5Z-O8CWx4Wpx$!v)EY_=toq^r&+nt%+><8H&#u&G!ev>hSF{VL5P-F zBvrn@4)WW3UY3l#@ay+C8t=SPt*xxCX}YfI8~TkiiZds5(`jP6lPheUx#`GL{5+1s zPwsjb!7%F^TgP#fx7~fZk{(=hpoEV?zD=! znRoUZzz^C*p=;ZF&7;%7-NR%4R6M^(aXDx&Y*XP3UV(7dyLTp07)Q6FHS-aY0;B*b zKng6c0_xn*)|XcTS~)2|3jCk|?hhsuL`z{QQEVMh;q@8)HAFPv_?AF46bTj zFSGEGKc7M^Qh*frXA1CI*Xi1@D0Q}eTOOXZCX@jbG>j{efB~Po1V97#ksaZbeiJ#w YSqe*uI12WQbU?ZY2tlYK1%5$+4_ewzF8}}l From 1459754e65ff75732d904b7ad39afaccabab674e Mon Sep 17 00:00:00 2001 From: Peter Carbonetto Date: Wed, 15 Mar 2023 14:52:20 -0500 Subject: [PATCH 25/50] Added fastTopics to READMEs. --- README.md | 2 +- docs/README.md | 1 + 2 files changed, 2 insertions(+), 1 deletion(-) diff --git a/README.md b/README.md index 6a17b40..645b005 100644 --- a/README.md +++ b/README.md @@ -29,7 +29,7 @@ remotes::install_github('satijalab/seurat-wrappers') | schex | [Using schex with Seurat](http://htmlpreview.github.io/?https://github.com/satijalab/seurat-wrappers/blob/master/docs/schex.html) | Freytag, R package 2019 | https://github.com/SaskiaFreytag/schex | | alevin | [Import alevin counts into Seurat](http://htmlpreview.github.io/?https://github.com/satijalab/seurat-wrappers/blob/master/docs/alevin.html) | Srivastava et. al., Genome Biology 2019 | https://github.com/k3yavi/alevin-Rtools | | Nebulosa | [Visualization of gene expression with Nebulosa](http://htmlpreview.github.io/?https://github.com/satijalab/seurat-wrappers/blob/master/docs/nebulosa.html) | Jose Alquicira-Hernandez and Joseph E. Powell, _Under Review_ | https://github.com/powellgenomicslab/Nebulosa | -| fastTopics | [Analyzing Seurat data using fastTopics](http://htmlpreview.github.io/?https://github.com/stephenslab/seurat-wrappers/blob/master/docs/fasttopics.html) | Peter Carbonetto and Matthew Stephens, _Under Review_ | https://github.com/stephenslab/fastTopics | +| fastTopics | [Analyzing Seurat data using fastTopics](http://htmlpreview.github.io/?https://github.com/stephenslab/seurat-wrappers/blob/master/docs/fasttopics.html) | Peter Carbonetto et al, bioRxiv 2023 | https://github.com/stephenslab/fastTopics | | CIPR | [Using CIPR with human PBMC data](http://htmlpreview.github.io/?https://github.com/satijalab/seurat-wrappers/blob/master/docs/cipr.html) | Ekiz et. al., BMC Bioinformatics 2020 | https://github.com/atakanekiz/CIPR-Package | | miQC | [Running miQC on Seurat objects](http://htmlpreview.github.io/?https://github.com/satijalab/seurat-wrappers/blob/master/docs/miQC.html) | Hippen et. al., bioRxiv 2021 | https://github.com/greenelab/miQC | | tricycle | [Running estimate_cycle_position from tricycle on Seurat Objects](http://htmlpreview.github.io/?https://github.com/satijalab/seurat-wrappers/blob/master/docs/tricycle.html) | Zheng et. al., bioRxiv 2021 | https://www.bioconductor.org/packages/release/bioc/html/tricycle.html | diff --git a/docs/README.md b/docs/README.md index 3f88476..90e17a2 100644 --- a/docs/README.md +++ b/docs/README.md @@ -15,3 +15,4 @@ This directory contains individual method vignettes for each method provided by | Harmony | [Integration of datasets using Harmony](http://htmlpreview.github.io/?https://github.com/satijalab/seurat-wrappers/blob/master/docs/harmony.html) | Korsunsky et al, bioRxiv 2018 | https://github.com/immunogenomics/harmony | | ALRA | [Zero-preserving imputation with ALRA](http://htmlpreview.github.io/?https://github.com/satijalab/seurat-wrappers/blob/master/docs/alra.html) | Linderman et al, bioRxiv 2018 | https://github.com/KlugerLab/ALRA | | Velocity | [Estimating RNA Velocity using Seurat](http://htmlpreview.github.io/?https://github.com/satijalab/seurat-wrappers/blob/master/docs/velocity.html) | La Manno et al, Nature 2018 | https://velocyto.org | +| fastTopics | [](http://htmlpreview.github.io/?https://github.com/stephenslab/seurat-wrappers/blob/master/docs/fasttopics.html) | Carbonetto et al, bioRxiv 2023 | https://github.com/stephenslab/fastTopics | From 91d130be56fc4430d3c3e1aba0763d3df76f72a0 Mon Sep 17 00:00:00 2001 From: Peter Carbonetto Date: Wed, 15 Mar 2023 15:01:40 -0500 Subject: [PATCH 26/50] Added link to README. --- docs/README.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/docs/README.md b/docs/README.md index 90e17a2..fa82087 100644 --- a/docs/README.md +++ b/docs/README.md @@ -15,4 +15,4 @@ This directory contains individual method vignettes for each method provided by | Harmony | [Integration of datasets using Harmony](http://htmlpreview.github.io/?https://github.com/satijalab/seurat-wrappers/blob/master/docs/harmony.html) | Korsunsky et al, bioRxiv 2018 | https://github.com/immunogenomics/harmony | | ALRA | [Zero-preserving imputation with ALRA](http://htmlpreview.github.io/?https://github.com/satijalab/seurat-wrappers/blob/master/docs/alra.html) | Linderman et al, bioRxiv 2018 | https://github.com/KlugerLab/ALRA | | Velocity | [Estimating RNA Velocity using Seurat](http://htmlpreview.github.io/?https://github.com/satijalab/seurat-wrappers/blob/master/docs/velocity.html) | La Manno et al, Nature 2018 | https://velocyto.org | -| fastTopics | [](http://htmlpreview.github.io/?https://github.com/stephenslab/seurat-wrappers/blob/master/docs/fasttopics.html) | Carbonetto et al, bioRxiv 2023 | https://github.com/stephenslab/fastTopics | +| fastTopics | [Analyzing Seurat data using fastTopics](http://htmlpreview.github.io/?https://github.com/stephenslab/seurat-wrappers/blob/master/docs/fasttopics.html) | Carbonetto et al, bioRxiv 2023 | https://github.com/stephenslab/fastTopics | From 6d1ac21a9880715b5bf2281c7923621554442afe Mon Sep 17 00:00:00 2001 From: Peter Carbonetto Date: Wed, 15 Mar 2023 15:29:00 -0500 Subject: [PATCH 27/50] Updated citations in fasttopics vignette. --- docs/fasttopics.Rmd | 27 ++++++++++++++++++--------- 1 file changed, 18 insertions(+), 9 deletions(-) diff --git a/docs/fasttopics.Rmd b/docs/fasttopics.Rmd index 7bcfa01..69f4930 100644 --- a/docs/fasttopics.Rmd +++ b/docs/fasttopics.Rmd @@ -4,10 +4,10 @@ date: 'Compiled: `r format(Sys.Date(), "%B %d, %Y")`' output: github_document: html_preview: true + toc: true + toc_depth: 3 html_document: df_print: kable - highlight: textmate - theme: readable --- Here we illustrate the use of the [fastTopics][fasttopics] Seurat @@ -20,14 +20,21 @@ a topic model, please see the If you find the **fastTopics** package useful for your work, please cite: -Kushal K. Dey, Chiaowen Joyce Hsiao and Matthew Stephens (2017). -[Visualizing the structure of RNA-seq expression data using grade of -membership models.][countclust-paper] *PLoS Genetics* **13**, e1006599. +K. K. Dey, C. Joyce Hsiao and M. Stephens (2017). [Visualizing the +structure of RNA-seq expression data using grade of membership +models.][countclust-paper] PLoS Genetics 13, e1006599. -Peter Carbonetto, Kevin Luo, Kushal Dey, Joyce Hsiao and Matthew -Stephens (2021). fastTopics: fast algorithms for fitting topic models -and non-negative matrix factorizations to count data. R package -version 0.4-23. [https://github.com/stephenslab/fastTopics][fasttopics] +P. Carbonetto, A. Sarkar, Z. Wang and M. Stephens (2021). +[Non-negative matrix factorization algorithms greatly improve topic +model fits.][fasttopics-paper] arXiv 2105.13440 + +If you used the `de_analysis` function in fastTopics, please cite: + +P. Carbonetto, K. Luo, A. Sarkar, A. Hung, K. Tayeb, S. Pott and +M. Stephens (2023). [Interpreting structure in sequence count data +with differential expression analysis allowing for grades of +membership.][single-cell-topics-paper] +bioRxiv doi:10.1101/2023.03.03.531029 ```{r knitr-opts, include=FALSE} knitr::opts_chunk$set(comment = "#",collapse = TRUE,results = "hold", @@ -119,3 +126,5 @@ sessionInfo() [fasttopics]: https://github.com/stephenslab/fastTopics [fasttopics-vignettes]: https://stephenslab.github.io/fastTopics/articles [countclust-paper]: https://doi.org/10.1371/journal.pgen.1006599 +[fasttopics-paper]: https://arxiv.org/abs/2105.13440 +[single-cell-topics-paper]: https://doi.org/10.1101/2023.03.03.531029 From 88660d286445875f5452c2a4fb054befcd9498ec Mon Sep 17 00:00:00 2001 From: Peter Carbonetto Date: Wed, 15 Mar 2023 15:56:24 -0500 Subject: [PATCH 28/50] Rebuilt the fasttopics vignettes. --- DESCRIPTION | 2 +- NAMESPACE | 6 +- docs/fasttopics.html | 287 ++++++++++-------- docs/fasttopics.md | 243 +++++++-------- docs/fasttopics_files/figure-html/pca-1-1.png | Bin 129971 -> 0 bytes docs/fasttopics_files/figure-html/pca-2-1.png | Bin 149127 -> 0 bytes .../figure-html/structure-plot-1.png | Bin 101380 -> 0 bytes .../figure-markdown_strict/pca-1-1.png | Bin 0 -> 42381 bytes .../figure-markdown_strict/pca-2-1.png | Bin 0 -> 51039 bytes .../structure-plot-1.png | Bin 0 -> 35827 bytes man/RunPresto.Rd | 1 - man/RunPrestoAll.Rd | 1 - man/RunQuantileNorm.Rd | 23 -- 13 files changed, 288 insertions(+), 275 deletions(-) delete mode 100644 docs/fasttopics_files/figure-html/pca-1-1.png delete mode 100644 docs/fasttopics_files/figure-html/pca-2-1.png delete mode 100644 docs/fasttopics_files/figure-html/structure-plot-1.png create mode 100644 docs/fasttopics_files/figure-markdown_strict/pca-1-1.png create mode 100644 docs/fasttopics_files/figure-markdown_strict/pca-2-1.png create mode 100644 docs/fasttopics_files/figure-markdown_strict/structure-plot-1.png diff --git a/DESCRIPTION b/DESCRIPTION index 191f733..87e5a95 100644 --- a/DESCRIPTION +++ b/DESCRIPTION @@ -61,7 +61,7 @@ Collate: 'velocity.R' Encoding: UTF-8 LazyData: true -RoxygenNote: 7.1.1 +RoxygenNote: 7.1.2 Suggests: cipr, conos, diff --git a/NAMESPACE b/NAMESPACE index 60cc279..ecf8848 100644 --- a/NAMESPACE +++ b/NAMESPACE @@ -7,9 +7,9 @@ S3method(as.Seurat,cell_data_set) S3method(as.Seurat,list) S3method(as.cell_data_set,Seurat) export(ALRAChooseKPlot) +export(ExportToCellbrowser) export(FitPoissonNMF) export(FitTopicModel) -export(ExportToCellbrowser) export(PlotMiQC) export(ReadAlevin) export(ReadVelocity) @@ -67,11 +67,11 @@ importFrom(Seurat,as.Graph) importFrom(Seurat,as.Seurat) importFrom(Seurat,as.SingleCellExperiment) importFrom(cowplot,theme_cowplot) -importFrom(fastTopics,fit_poisson_nmf) -importFrom(fastTopics,fit_topic_model) importFrom(data.table,data.table) importFrom(data.table,fwrite) importFrom(data.table,setDTthreads) +importFrom(fastTopics,fit_poisson_nmf) +importFrom(fastTopics,fit_topic_model) importFrom(ggplot2,aes_string) importFrom(ggplot2,geom_line) importFrom(ggplot2,geom_point) diff --git a/docs/fasttopics.html b/docs/fasttopics.html index 1576e9a..44d9d43 100644 --- a/docs/fasttopics.html +++ b/docs/fasttopics.html @@ -13,26 +13,24 @@ Analyzing Seurat data using fastTopics - + - + - + code{white-space: pre-wrap;} + span.smallcaps{font-variant: small-caps;} + span.underline{text-decoration: underline;} + div.column{display: inline-block; vertical-align: top; width: 50%;} + div.hanging-indent{margin-left: 1.5em; text-indent: -1.5em;} + ul.task-list{list-style: none;} + + + diff --git a/docs/fasttopics.md b/docs/fasttopics.md index 81d9343..3f5a4e9 100644 --- a/docs/fasttopics.md +++ b/docs/fasttopics.md @@ -1,7 +1,3 @@ -Analyzing Seurat data using fastTopics -================ -Compiled: January 15, 2021 - Here we illustrate the use of the [fastTopics](https://github.com/stephenslab/fastTopics) Seurat wrapper to analyze a Seurat data set. This vignette is only intended to @@ -13,161 +9,166 @@ vignettes](https://stephenslab.github.io/fastTopics/articles). If you find the **fastTopics** package useful for your work, please cite: -Kushal K. Dey, Chiaowen Joyce Hsiao and Matthew Stephens (2017). -[Visualizing the structure of RNA-seq expression data using grade of -membership models.](https://doi.org/10.1371/journal.pgen.1006599) *PLoS -Genetics* **13**, e1006599. +K. K. Dey, C. Joyce Hsiao and M. Stephens (2017). [Visualizing the +structure of RNA-seq expression data using grade of membership +models.](https://doi.org/10.1371/journal.pgen.1006599) PLoS Genetics 13, +e1006599. + +P. Carbonetto, A. Sarkar, Z. Wang and M. Stephens (2021). [Non-negative +matrix factorization algorithms greatly improve topic model +fits.](https://arxiv.org/abs/2105.13440) arXiv 2105.13440 + +If you used the `de_analysis` function in fastTopics, please cite: -Peter Carbonetto, Kevin Luo, Kushal Dey, Joyce Hsiao and Matthew -Stephens (2021). fastTopics: fast algorithms for fitting topic models -and non-negative matrix factorizations to count data. R package version -0.4-23. +P. Carbonetto, K. Luo, A. Sarkar, A. Hung, K. Tayeb, S. Pott and M. +Stephens (2023). [Interpreting structure in sequence count data with +differential expression analysis allowing for grades of +membership.](https://doi.org/10.1101/2023.03.03.531029) bioRxiv + We begin by loading the packages used to perform the analysis. -``` r -library(Seurat) -library(SeuratData) -library(SeuratWrappers) -library(fastTopics) -library(cowplot) -``` + library(Seurat) + library(SeuratData) + library(SeuratWrappers) + library(fastTopics) + library(cowplot) We set the seed so that the results can be reproduced. -``` r -set.seed(1) -``` + set.seed(1) Load—and, if necessary, install—the PBMC 3k data set containing transcription profiles for 2,700 cells. -``` r -InstallData("pbmc3k") -data(pbmc3k) -dim(GetAssayData(pbmc3k)) -# [1] 13714 2700 -``` + InstallData("pbmc3k") + data(pbmc3k) + dim(GetAssayData(pbmc3k)) + # [1] 13714 2700 Fit the multinomial topic model to the raw UMI counts—*no pre-processing or pre-selection of genes is needed.* Note that it may take several minutes to complete this model fitting step. -``` r -pbmc3k <- FitTopicModel(pbmc3k,k = 6) -``` + pbmc3k <- FitTopicModel(pbmc3k,k = 6) -To fit a topic model, we must specify \(K\), the number of topics. Here, -we have chosen \(K = 6\) topics. In most settings, a good choice of -\(K\) will not be known in advance, so you will you want to explore the -results from topic models at different settings of \(K\). +To fit a topic model, we must specify *K*, the number of topics. Here, +we have chosen *K* = 6 topics. In most settings, a good choice of *K* +will not be known in advance, so you will you want to explore the +results from topic models at different settings of *K*. This plot shows the cells projected onto the top two principal components (PCs) of the topic mixture proportions. -``` r -Idents(pbmc3k) <- pbmc3k$seurat_annotations -DimPlot(pbmc3k,reduction = "pca_topics",pt.size = 1) + - theme_cowplot(font_size = 10) -``` + Idents(pbmc3k) <- pbmc3k$seurat_annotations + DimPlot(pbmc3k,reduction = "pca_topics",pt.size = 1) + + theme_cowplot(font_size = 10) - + Compare this against the top two PCs of the transformed counts: -``` r -pbmc3k <- FindVariableFeatures(pbmc3k) -pbmc3k <- NormalizeData(pbmc3k) -pbmc3k <- ScaleData(pbmc3k) -pbmc3k <- RunPCA(pbmc3k) -DimPlot(pbmc3k,reduction = "pca",pt.size = 1) + - theme_cowplot(font_size = 10) -``` + pbmc3k <- FindVariableFeatures(pbmc3k) + pbmc3k <- NormalizeData(pbmc3k) + pbmc3k <- ScaleData(pbmc3k) + pbmc3k <- RunPCA(pbmc3k) + DimPlot(pbmc3k,reduction = "pca",pt.size = 1) + + theme_cowplot(font_size = 10) - + The fitted topic model—a “multinom\_topic\_model” object—is stored in the “misc” slot: -``` r -fit <- Misc(Reductions(pbmc3k,"multinom_topic_model")) -``` + fit <- Misc(Reductions(pbmc3k,"multinom_topic_model")) Once the fitted topic model is extracted, many functions from the **fastTopics** package can be used for analysis and visualization. For example, the Structure plot provides an evocative visual summary of the estimated mixture proportions for each cell. Here, we have grouped the -cells by previously inferred -labels. +cells by previously inferred labels. -``` r -structure_plot(fit,grouping = Idents(pbmc3k),gap = 25) -``` + structure_plot(fit,grouping = Idents(pbmc3k),gap = 25) - + This is the version of R and the packages that were used to generate these results. -``` r -sessionInfo() -# R version 3.6.2 (2019-12-12) -# Platform: x86_64-apple-darwin15.6.0 (64-bit) -# Running under: macOS Catalina 10.15.7 -# -# Matrix products: default -# BLAS: /Library/Frameworks/R.framework/Versions/3.6/Resources/lib/libRblas.0.dylib -# LAPACK: /Library/Frameworks/R.framework/Versions/3.6/Resources/lib/libRlapack.dylib -# -# locale: -# [1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8 -# -# attached base packages: -# [1] stats graphics grDevices utils datasets methods base -# -# other attached packages: -# [1] cowplot_1.0.0 fastTopics_0.4-23 SeuratWrappers_0.3.2 -# [4] pbmc3k.SeuratData_3.1.4 SeuratData_0.2.1 Seurat_3.2.3 -# -# loaded via a namespace (and not attached): -# [1] Rtsne_0.15 colorspace_1.4-1 deldir_0.1-29 -# [4] ggridges_0.5.2 spatstat.data_1.4-3 farver_2.0.1 -# [7] leiden_0.3.3 listenv_0.8.0 remotes_2.1.0 -# [10] MatrixModels_0.4-1 ggrepel_0.9.0 fansi_0.4.0 -# [13] codetools_0.2-16 splines_3.6.2 knitr_1.26 -# [16] polyclip_1.10-0 zeallot_0.1.0 jsonlite_1.6 -# [19] mcmc_0.9-6 ica_1.0-2 cluster_2.1.0 -# [22] png_0.1-7 uwot_0.1.10 shiny_1.4.0 -# [25] sctransform_0.3.2 BiocManager_1.30.10 compiler_3.6.2 -# [28] httr_1.4.2 backports_1.1.5 assertthat_0.2.1 -# [31] Matrix_1.2-18 fastmap_1.0.1 lazyeval_0.2.2 -# [34] cli_2.0.0 later_1.0.0 prettyunits_1.1.1 -# [37] htmltools_0.4.0 quantreg_5.54 tools_3.6.2 -# [40] rsvd_1.0.2 igraph_1.2.5 coda_0.19-3 -# [43] gtable_0.3.0 glue_1.3.1 RANN_2.6.1 -# [46] reshape2_1.4.3 dplyr_0.8.3 rappdirs_0.3.1 -# [49] Rcpp_1.0.5 spatstat_1.64-1 scattermore_0.7 -# [52] vctrs_0.2.1 nlme_3.1-142 lmtest_0.9-38 -# [55] xfun_0.11 stringr_1.4.0 globals_0.13.0 -# [58] mime_0.8 miniUI_0.1.1.1 lifecycle_0.1.0 -# [61] irlba_2.3.3 goftest_1.2-2 future_1.18.0 -# [64] MASS_7.3-51.4 zoo_1.8-7 scales_1.1.0 -# [67] hms_0.5.2 promises_1.1.0 spatstat.utils_1.17-0 -# [70] parallel_3.6.2 SparseM_1.78 RColorBrewer_1.1-2 -# [73] yaml_2.2.0 reticulate_1.16 pbapply_1.4-3 -# [76] gridExtra_2.3 ggplot2_3.3.0 rpart_4.1-15 -# [79] stringi_1.4.3 rlang_0.4.5 pkgconfig_2.0.3 -# [82] matrixStats_0.56.0 evaluate_0.14 lattice_0.20-38 -# [85] ROCR_1.0-11 purrr_0.3.3 tensor_1.5 -# [88] labeling_0.3 patchwork_1.0.1 htmlwidgets_1.5.1 -# [91] tidyselect_0.2.5 RcppAnnoy_0.0.18 plyr_1.8.5 -# [94] magrittr_1.5 R6_2.4.1 pillar_1.4.3 -# [97] mgcv_1.8-31 fitdistrplus_1.1-1 survival_3.1-8 -# [100] abind_1.4-5 tibble_2.1.3 future.apply_1.6.0 -# [103] crayon_1.3.4 KernSmooth_2.23-16 plotly_4.9.2 -# [106] rmarkdown_2.3 progress_1.2.2 grid_3.6.2 -# [109] data.table_1.12.8 digest_0.6.23 xtable_1.8-4 -# [112] tidyr_1.0.0 httpuv_1.5.2 MCMCpack_1.4-5 -# [115] RcppParallel_4.4.2 munsell_0.5.0 viridisLite_0.3.0 -# [118] quadprog_1.5-8 -``` + sessionInfo() + # R version 4.2.0 (2022-04-22) + # Platform: x86_64-pc-linux-gnu (64-bit) + # Running under: Red Hat Enterprise Linux 8.4 (Ootpa) + # + # Matrix products: default + # BLAS/LAPACK: /software/openblas-0.3.13-el8-x86_64/lib/libopenblas_skylakexp-r0.3.13.so + # + # locale: + # [1] LC_CTYPE=en_US.UTF-8 LC_NUMERIC=C + # [3] LC_TIME=en_US.UTF-8 LC_COLLATE=en_US.UTF-8 + # [5] LC_MONETARY=en_US.UTF-8 LC_MESSAGES=en_US.UTF-8 + # [7] LC_PAPER=en_US.UTF-8 LC_NAME=C + # [9] LC_ADDRESS=C LC_TELEPHONE=C + # [11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C + # + # attached base packages: + # [1] stats graphics grDevices utils datasets methods base + # + # other attached packages: + # [1] rmarkdown_2.14 SeuratWrappers_0.3.1 pbmc3k.SeuratData_3.1.4 + # [4] cowplot_1.1.1 fastTopics_0.6-150 SeuratData_0.2.2 + # [7] SeuratObject_4.1.3 Seurat_4.3.0 + # + # loaded via a namespace (and not attached): + # [1] utf8_1.2.2 spatstat.explore_3.0-6 reticulate_1.24 + # [4] R.utils_2.11.0 tidyselect_1.1.2 htmlwidgets_1.5.4 + # [7] grid_4.2.0 Rtsne_0.16 devtools_2.4.3 + # [10] munsell_0.5.0 codetools_0.2-18 ica_1.0-2 + # [13] future_1.25.0 miniUI_0.1.1.1 withr_2.5.0 + # [16] spatstat.random_3.1-3 colorspace_2.0-3 progressr_0.10.0 + # [19] highr_0.9 knitr_1.39 rstudioapi_0.13 + # [22] ROCR_1.0-11 tensor_1.5 listenv_0.8.0 + # [25] labeling_0.4.2 mixsqp_0.3-48 polyclip_1.10-0 + # [28] MCMCpack_1.6-3 farver_2.1.0 rprojroot_2.0.3 + # [31] coda_0.19-4 parallelly_1.31.1 vctrs_0.4.1 + # [34] generics_0.1.2 xfun_0.30 R6_2.5.1 + # [37] rsvd_1.0.5 invgamma_1.1 spatstat.utils_3.0-1 + # [40] cachem_1.0.6 assertthat_0.2.1 promises_1.2.0.1 + # [43] scales_1.2.0 gtable_0.3.0 globals_0.14.0 + # [46] processx_3.5.3 goftest_1.2-3 mcmc_0.9-7 + # [49] rlang_1.0.2 MatrixModels_0.5-0 splines_4.2.0 + # [52] lazyeval_0.2.2 spatstat.geom_3.0-6 BiocManager_1.30.20 + # [55] yaml_2.3.5 reshape2_1.4.4 abind_1.4-5 + # [58] httpuv_1.6.5 tools_4.2.0 usethis_2.1.5 + # [61] ggplot2_3.3.6 ellipsis_0.3.2 jquerylib_0.1.4 + # [64] RColorBrewer_1.1-3 sessioninfo_1.2.2 ggridges_0.5.3 + # [67] Rcpp_1.0.9 plyr_1.8.7 progress_1.2.2 + # [70] purrr_0.3.4 ps_1.7.0 prettyunits_1.1.1 + # [73] deldir_1.0-6 pbapply_1.5-0 ashr_2.2-54 + # [76] zoo_1.8-10 ggrepel_0.9.1 cluster_2.1.3 + # [79] fs_1.5.2 magrittr_2.0.3 data.table_1.14.4 + # [82] scattermore_0.8 SparseM_1.81 lmtest_0.9-40 + # [85] RANN_2.6.1 truncnorm_1.0-8 SQUAREM_2021.1 + # [88] fitdistrplus_1.1-8 matrixStats_0.62.0 pkgload_1.2.4 + # [91] hms_1.1.1 patchwork_1.1.1 mime_0.12 + # [94] evaluate_0.15 xtable_1.8-4 gridExtra_2.3 + # [97] testthat_3.1.4 compiler_4.2.0 tibble_3.1.7 + # [100] KernSmooth_2.23-20 crayon_1.5.1 R.oo_1.24.0 + # [103] htmltools_0.5.2 later_1.3.0 tidyr_1.2.0 + # [106] RcppParallel_5.1.5 DBI_1.1.2 MASS_7.3-56 + # [109] rappdirs_0.3.3 Matrix_1.5-3 brio_1.1.3 + # [112] cli_3.3.0 quadprog_1.5-8 R.methodsS3_1.8.1 + # [115] parallel_4.2.0 igraph_1.3.1 pkgconfig_2.0.3 + # [118] sp_1.6-0 plotly_4.10.0 spatstat.sparse_3.0-0 + # [121] xml2_1.3.3 roxygen2_7.1.2 bslib_0.3.1 + # [124] stringr_1.4.0 callr_3.7.0 digest_0.6.29 + # [127] sctransform_0.3.5 RcppAnnoy_0.0.19 spatstat.data_3.0-0 + # [130] leiden_0.3.10 uwot_0.1.14 shiny_1.7.1 + # [133] quantreg_5.93 lifecycle_1.0.1 nlme_3.1-157 + # [136] jsonlite_1.8.0 desc_1.4.1 viridisLite_0.4.0 + # [139] fansi_1.0.3 pillar_1.7.0 lattice_0.20-45 + # [142] fastmap_1.1.0 httr_1.4.2 pkgbuild_1.3.1 + # [145] survival_3.3-1 glue_1.6.2 remotes_2.4.2 + # [148] png_0.1-7 stringi_1.7.6 sass_0.4.1 + # [151] memoise_2.0.1 dplyr_1.0.9 irlba_2.3.5 + # [154] future.apply_1.9.0 diff --git a/docs/fasttopics_files/figure-html/pca-1-1.png b/docs/fasttopics_files/figure-html/pca-1-1.png deleted file mode 100644 index 7a4899d19a064482a2d06257cedfc4003416ce1b..0000000000000000000000000000000000000000 GIT binary patch literal 0 HcmV?d00001 literal 129971 zcmeFZRajMF+b)VoBVE!c-AXqo4bt66H%ND?h;&PXNOyM$(%s!LX_z#V*aN|}*7twc 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zV9LPYpqrBu77h-_g9lfz837SwCM57ZpZo-wQ@z(|?1sz@M37>R3RDMZ0{&M~vOX2& zVt_@%W5p*RNA&-9H3xtG1V=`SIjzV*r36TJM#2z~fL&*V0G?ot2xxhqPjXq8ArM|z zV-y|5AH*TsCIUt=54=_@BuoH4J$U>osf_>DQyo1o&%C?A;138|B; zfncoVgTU)F2+ZKV+OA8==gY`rw6>j1SHK1si@(2A`h`7mJ9cXAtxL+ zB&7r)raU`Im?Ry4nqs~h0TT8Sgacsxzd^G}&AiV&=wG$8`~vwEWa_YE!NuI6e1NFM zP#3;2L-CBpp7iQpBQ%vx_=9-i3NMSMzJ1P4YY85;aH_&z=+q@kby0SyNyNf-za z{`W^^-=(2OE4>3%ZxH1+s;uY1+A;}|AoMWd?%;PDcS9sw%y~j0QQnZC{P*SXCD_g* zsPGyB(IUZf{U!tg^M5Yv`nCT)3O Date: Wed, 15 Mar 2023 16:43:58 -0500 Subject: [PATCH 29/50] Fixed the formatting of the citations in the fasttopics vignette. --- docs/fasttopics.Rmd | 102 +++++++++++++++++++++++++------------------- 1 file changed, 59 insertions(+), 43 deletions(-) diff --git a/docs/fasttopics.Rmd b/docs/fasttopics.Rmd index 69f4930..808eddf 100644 --- a/docs/fasttopics.Rmd +++ b/docs/fasttopics.Rmd @@ -10,38 +10,55 @@ output: df_print: kable --- -Here we illustrate the use of the [fastTopics][fasttopics] Seurat -wrapper to analyze a Seurat data set. This vignette is only intended -to introduce the basic fastTopics interface for Seurat objects---for -background and guidance on analysis of single-cell RNA-seq data using -a topic model, please see the +```{r knitr-opts, include=FALSE} +knitr::opts_chunk$set(comment = "#",collapse = TRUE,results = "hold", + fig.align = "center",dpi = 120,tidy = TRUE, + tidy.opts = list(width.cutoff = 95)) +``` + +This vignette illustrates the use of the [fastTopics][fasttopics] +Seurat wrapper to analyze a Seurat data set. This vignette is only +intended to introduce the basic fastTopics interface for Seurat +objects---for more guidance on analyzing single-cell RNA-seq data +using a topic model, please see the [fastTopics vignettes][fasttopics-vignettes]. If you find the **fastTopics** package useful for your work, please cite: -K. K. Dey, C. Joyce Hsiao and M. Stephens (2017). [Visualizing the -structure of RNA-seq expression data using grade of membership -models.][countclust-paper] PLoS Genetics 13, e1006599. - -P. Carbonetto, A. Sarkar, Z. Wang and M. Stephens (2021). -[Non-negative matrix factorization algorithms greatly improve topic -model fits.][fasttopics-paper] arXiv 2105.13440 - -If you used the `de_analysis` function in fastTopics, please cite: - -P. Carbonetto, K. Luo, A. Sarkar, A. Hung, K. Tayeb, S. Pott and -M. Stephens (2023). [Interpreting structure in sequence count data -with differential expression analysis allowing for grades of -membership.][single-cell-topics-paper] -bioRxiv doi:10.1101/2023.03.03.531029 - -```{r knitr-opts, include=FALSE} -knitr::opts_chunk$set(comment = "#",collapse = TRUE,results = "hold", - fig.align = "center",dpi = 120) -``` - -We begin by loading the packages used to perform the analysis. +> *Visualizing the structure of RNA-seq expression data using grade of +> membership models.* +> +> K. K. Dey, C. Joyce Hsiao and M. Stephens. +> +> PLoS Genetics, 2017. +> +> doi: [10.1371/journal.pgen.1006599][countclust-paper] + +and + +> Non-negative matrix factorization algorithms greatly improve topic +> model fits. +> +> P. Carbonetto, A. Sarkar, Z. Wang and M. Stephens. +> +> arXiv, 2021 +> +> [arXiv 2105.13440][https://arxiv.org/abs/2105.13440] + +If you use the **de_analysis** function in fastTopics, please cite: + +> Interpreting structure in sequence count data with differential +> expression analysis allowing for grades of membership. +> +> P. Carbonetto, K. Luo, A. Sarkar, A. Hung, K. Tayeb, S. Pott and +> M. Stephens +> +> bioRxiv, 2023. +> +> doi: [10.1101/2023.03.03.531029][https://doi.org/10.1101/2023.03.03.531029] + +To begin, loading the packages used to perform the analysis. ```{r load-pkgs, message=FALSE, warning=FALSE} library(Seurat) @@ -51,13 +68,13 @@ library(fastTopics) library(cowplot) ``` -We set the seed so that the results can be reproduced. +And set the seed so that the results can be reproduced. ```{r set-seed} set.seed(1) ``` -Load---and, if necessary, install---the PBMC 3k data set containing +Load and, if necessary, install the PBMC 3k data set containing transcription profiles for 2,700 cells. ```{r load-data, warning=FALSE} @@ -66,9 +83,9 @@ data(pbmc3k) dim(GetAssayData(pbmc3k)) ``` -Fit the multinomial topic model to the raw UMI counts---*no -pre-processing or pre-selection of genes is needed.* Note that it may -take several minutes to complete this model fitting step. +Fit the multinomial topic model to the *raw UMI counts.* **No +preprocessing or pre-selection of genes is needed.** It may +take several minutes to fit the model. ```{r fit-model, results="hide", message=FALSE} pbmc3k <- FitTopicModel(pbmc3k,k = 6) @@ -79,8 +96,8 @@ Here, we have chosen $K = 6$ topics. In most settings, a good choice of $K$ will not be known in advance, so you will you want to explore the results from topic models at different settings of $K$. -This plot shows the cells projected onto the top two principal -components (PCs) of the topic mixture proportions. +This first plot shows the cells projected onto the top two PCs of the +topic proportions. ```{r pca-1, fig.width=4.5, fig.height=3} Idents(pbmc3k) <- pbmc3k$seurat_annotations @@ -106,18 +123,20 @@ in the "misc" slot: fit <- Misc(Reductions(pbmc3k,"multinom_topic_model")) ``` -Once the fitted topic model is extracted, many functions from the -**fastTopics** package can be used for analysis and visualization. For -example, the Structure plot provides an evocative visual summary of -the estimated mixture proportions for each cell. Here, we have grouped -the cells by previously inferred labels. +Once the fitted topic model is extracted, many fastTopics functions +can be used for analysis and visualization. For example, the Structure +plot provides an evocative visual summary of the estimated mixture +proportions for each cell. Here, we have grouped the cells by +previously inferred labels. ```{r structure-plot, fig.width=7.5, fig.height=1.75, results="hide", message=FALSE} structure_plot(fit,grouping = Idents(pbmc3k),gap = 25) ``` +TO DO: Illustrate the use of the de_analysis. + This is the version of R and the packages that were used to generate -these results. +these results: ```{r session-info} sessionInfo() @@ -125,6 +144,3 @@ sessionInfo() [fasttopics]: https://github.com/stephenslab/fastTopics [fasttopics-vignettes]: https://stephenslab.github.io/fastTopics/articles -[countclust-paper]: https://doi.org/10.1371/journal.pgen.1006599 -[fasttopics-paper]: https://arxiv.org/abs/2105.13440 -[single-cell-topics-paper]: https://doi.org/10.1101/2023.03.03.531029 From 4304d5e63b793e65ae4ac296e4881c0e18945ac3 Mon Sep 17 00:00:00 2001 From: Peter Carbonetto Date: Wed, 15 Mar 2023 16:47:59 -0500 Subject: [PATCH 30/50] A few small edits to the fasttopics vignette. --- docs/fasttopics.Rmd | 8 ++++---- 1 file changed, 4 insertions(+), 4 deletions(-) diff --git a/docs/fasttopics.Rmd b/docs/fasttopics.Rmd index 808eddf..47758fb 100644 --- a/docs/fasttopics.Rmd +++ b/docs/fasttopics.Rmd @@ -83,9 +83,9 @@ data(pbmc3k) dim(GetAssayData(pbmc3k)) ``` -Fit the multinomial topic model to the *raw UMI counts.* **No -preprocessing or pre-selection of genes is needed.** It may -take several minutes to fit the model. +Fit the multinomial topic model to the raw UMI counts. *No data +preprocessing or gene filtering is needed.* It may take several +minutes to fit the model. ```{r fit-model, results="hide", message=FALSE} pbmc3k <- FitTopicModel(pbmc3k,k = 6) @@ -125,7 +125,7 @@ fit <- Misc(Reductions(pbmc3k,"multinom_topic_model")) Once the fitted topic model is extracted, many fastTopics functions can be used for analysis and visualization. For example, the Structure -plot provides an evocative visual summary of the estimated mixture +plot provides an evocative visual summary of the estimated topic proportions for each cell. Here, we have grouped the cells by previously inferred labels. From c48f7df885f19dcbea13d1ce9195ce619584b171 Mon Sep 17 00:00:00 2001 From: Peter Carbonetto Date: Wed, 15 Mar 2023 16:53:09 -0500 Subject: [PATCH 31/50] Rebuilt html for fasttopics vignette. --- docs/fasttopics.html | 116 ++++++++++++++++++++++++------------------- 1 file changed, 64 insertions(+), 52 deletions(-) diff --git a/docs/fasttopics.html b/docs/fasttopics.html index 44d9d43..970c643 100644 --- a/docs/fasttopics.html +++ b/docs/fasttopics.html @@ -367,75 +367,87 @@

Compiled: March 15, 2023

-

Here we illustrate the use of the fastTopics Seurat +

This vignette illustrates the use of the fastTopics Seurat wrapper to analyze a Seurat data set. This vignette is only intended to -introduce the basic fastTopics interface for Seurat objects—for -background and guidance on analysis of single-cell RNA-seq data using a -topic model, please see the fastTopics +introduce the basic fastTopics interface for Seurat objects—for more +guidance on analyzing single-cell RNA-seq data using a topic model, +please see the fastTopics vignettes.

If you find the fastTopics package useful for your work, please cite:

-

K. K. Dey, C. Joyce Hsiao and M. Stephens (2017). Visualizing the -structure of RNA-seq expression data using grade of membership -models. PLoS Genetics 13, e1006599.

-

P. Carbonetto, A. Sarkar, Z. Wang and M. Stephens (2021). Non-negative matrix -factorization algorithms greatly improve topic model fits. arXiv -2105.13440

-

If you used the de_analysis function in fastTopics, +

+

Visualizing the structure of RNA-seq expression data using grade +of membership models.

+

K. K. Dey, C. Joyce Hsiao and M. Stephens.

+

PLoS Genetics, 2017.

+

doi: [10.1371/journal.pgen.1006599][countclust-paper]

+
+

and

+
+

Non-negative matrix factorization algorithms greatly improve topic +model fits.

+

P. Carbonetto, A. Sarkar, Z. Wang and M. Stephens.

+

arXiv, 2021

+

[arXiv 2105.13440][https://arxiv.org/abs/2105.13440]

+
+

If you use the de_analysis function in fastTopics, please cite:

+
+

Interpreting structure in sequence count data with differential +expression analysis allowing for grades of membership.

P. Carbonetto, K. Luo, A. Sarkar, A. Hung, K. Tayeb, S. Pott and M. -Stephens (2023). Interpreting structure -in sequence count data with differential expression analysis allowing -for grades of membership. bioRxiv doi:10.1101/2023.03.03.531029

-

We begin by loading the packages used to perform the analysis.

+Stephens

+

bioRxiv, 2023.

+

doi: [10.1101/2023.03.03.531029][https://doi.org/10.1101/2023.03.03.531029]

+
+

To begin, loading the packages used to perform the analysis.

library(Seurat)
 library(SeuratData)
 library(SeuratWrappers)
 library(fastTopics)
 library(cowplot)
-

We set the seed so that the results can be reproduced.

+

And set the seed so that the results can be reproduced.

set.seed(1)
-

Load—and, if necessary, install—the PBMC 3k data set containing +

Load and, if necessary, install the PBMC 3k data set containing transcription profiles for 2,700 cells.

InstallData("pbmc3k")
 data(pbmc3k)
 dim(GetAssayData(pbmc3k))
 # [1] 13714  2700
-

Fit the multinomial topic model to the raw UMI counts—no -pre-processing or pre-selection of genes is needed. Note that it -may take several minutes to complete this model fitting step.

-
pbmc3k <- FitTopicModel(pbmc3k,k = 6)
+

Fit the multinomial topic model to the raw UMI counts. +No preprocessing or pre-selection of genes is needed. +It may take several minutes to fit the model.

+
pbmc3k <- FitTopicModel(pbmc3k, k = 6)

To fit a topic model, we must specify \(K\), the number of topics. Here, we have chosen \(K = 6\) topics. In most settings, a good choice of \(K\) will not be known in advance, so you will you want to explore the results from topic models at different settings of \(K\).

-

This plot shows the cells projected onto the top two principal -components (PCs) of the topic mixture proportions.

+

This first plot shows the cells projected onto the top two PCs of the +topic proportions.

Idents(pbmc3k) <- pbmc3k$seurat_annotations
-DimPlot(pbmc3k,reduction = "pca_topics",pt.size = 1) +
-  theme_cowplot(font_size = 10)
+DimPlot(pbmc3k, reduction = "pca_topics", pt.size = 1) + theme_cowplot(font_size = 10)

Compare this against the top two PCs of the transformed counts:

pbmc3k <- FindVariableFeatures(pbmc3k)
 pbmc3k <- NormalizeData(pbmc3k)
 pbmc3k <- ScaleData(pbmc3k)
 pbmc3k <- RunPCA(pbmc3k)
-DimPlot(pbmc3k,reduction = "pca",pt.size = 1) +
-  theme_cowplot(font_size = 10)
+DimPlot(pbmc3k, reduction = "pca", pt.size = 1) + theme_cowplot(font_size = 10)

The fitted topic model—a “multinom_topic_model” object—is stored in the “misc” slot:

-
fit <- Misc(Reductions(pbmc3k,"multinom_topic_model"))
-

Once the fitted topic model is extracted, many functions from the -fastTopics package can be used for analysis and -visualization. For example, the Structure plot provides an evocative -visual summary of the estimated mixture proportions for each cell. Here, -we have grouped the cells by previously inferred labels.

-
structure_plot(fit,grouping = Idents(pbmc3k),gap = 25)
+
fit <- Misc(Reductions(pbmc3k, "multinom_topic_model"))
+

Once the fitted topic model is extracted, many fastTopics functions +can be used for analysis and visualization. For example, the Structure +plot provides an evocative visual summary of the estimated mixture +proportions for each cell. Here, we have grouped the cells by previously +inferred labels.

+
structure_plot(fit, grouping = Idents(pbmc3k), gap = 25)

+

TO DO: Illustrate the use of the de_analysis.

This is the version of R and the packages that were used to generate -these results.

+these results:

sessionInfo()
 # R version 4.2.0 (2022-04-22)
 # Platform: x86_64-pc-linux-gnu (64-bit)
@@ -496,23 +508,23 @@ 
Compiled: March 15, 2023

 #  [97] testthat_3.1.4         compiler_4.2.0         tibble_3.1.7          
 # [100] KernSmooth_2.23-20     crayon_1.5.1           R.oo_1.24.0           
 # [103] htmltools_0.5.2        later_1.3.0            tidyr_1.2.0           
-# [106] RcppParallel_5.1.5     DBI_1.1.2              MASS_7.3-56           
-# [109] rappdirs_0.3.3         Matrix_1.5-3           brio_1.1.3            
-# [112] cli_3.3.0              quadprog_1.5-8         R.methodsS3_1.8.1     
-# [115] parallel_4.2.0         igraph_1.3.1           pkgconfig_2.0.3       
-# [118] sp_1.6-0               plotly_4.10.0          spatstat.sparse_3.0-0 
-# [121] xml2_1.3.3             roxygen2_7.1.2         bslib_0.3.1           
-# [124] stringr_1.4.0          callr_3.7.0            digest_0.6.29         
-# [127] sctransform_0.3.5      RcppAnnoy_0.0.19       spatstat.data_3.0-0   
-# [130] leiden_0.3.10          uwot_0.1.14            shiny_1.7.1           
-# [133] quantreg_5.93          lifecycle_1.0.1        nlme_3.1-157          
-# [136] jsonlite_1.8.0         desc_1.4.1             viridisLite_0.4.0     
-# [139] fansi_1.0.3            pillar_1.7.0           lattice_0.20-45       
-# [142] fastmap_1.1.0          httr_1.4.2             pkgbuild_1.3.1        
-# [145] survival_3.3-1         glue_1.6.2             remotes_2.4.2         
-# [148] png_0.1-7              stringi_1.7.6          sass_0.4.1            
-# [151] memoise_2.0.1          dplyr_1.0.9            irlba_2.3.5           
-# [154] future.apply_1.9.0
+# [106] RcppParallel_5.1.5 DBI_1.1.2 formatR_1.12 +# [109] MASS_7.3-56 rappdirs_0.3.3 Matrix_1.5-3 +# [112] brio_1.1.3 cli_3.3.0 quadprog_1.5-8 +# [115] R.methodsS3_1.8.1 parallel_4.2.0 igraph_1.3.1 +# [118] pkgconfig_2.0.3 sp_1.6-0 plotly_4.10.0 +# [121] spatstat.sparse_3.0-0 xml2_1.3.3 roxygen2_7.1.2 +# [124] bslib_0.3.1 stringr_1.4.0 callr_3.7.0 +# [127] digest_0.6.29 sctransform_0.3.5 RcppAnnoy_0.0.19 +# [130] spatstat.data_3.0-0 leiden_0.3.10 uwot_0.1.14 +# [133] shiny_1.7.1 quantreg_5.93 lifecycle_1.0.1 +# [136] nlme_3.1-157 jsonlite_1.8.0 desc_1.4.1 +# [139] viridisLite_0.4.0 fansi_1.0.3 pillar_1.7.0 +# [142] lattice_0.20-45 fastmap_1.1.0 httr_1.4.2 +# [145] pkgbuild_1.3.1 survival_3.3-1 glue_1.6.2 +# [148] remotes_2.4.2 png_0.1-7 stringi_1.7.6 +# [151] sass_0.4.1 memoise_2.0.1 dplyr_1.0.9 +# [154] irlba_2.3.5 future.apply_1.9.0 From 969f01aaab9b9719eae2df187dcbe3694b684632 Mon Sep 17 00:00:00 2001 From: Peter Carbonetto Date: Wed, 15 Mar 2023 20:15:57 -0500 Subject: [PATCH 32/50] A few small revisions to the fasttopics vignette. --- docs/fasttopics.Rmd | 42 ++++++++++++++++++++++-------------------- 1 file changed, 22 insertions(+), 20 deletions(-) diff --git a/docs/fasttopics.Rmd b/docs/fasttopics.Rmd index 47758fb..ddf245f 100644 --- a/docs/fasttopics.Rmd +++ b/docs/fasttopics.Rmd @@ -33,32 +33,32 @@ cite: > > PLoS Genetics, 2017. > -> doi: [10.1371/journal.pgen.1006599][countclust-paper] +> doi: [10.1371/journal.pgen.1006599](https://doi.org/10.1371/journal.pgen.1006599) and -> Non-negative matrix factorization algorithms greatly improve topic -> model fits. +> *Non-negative matrix factorization algorithms greatly improve topic +> model fits.* > > P. Carbonetto, A. Sarkar, Z. Wang and M. Stephens. > > arXiv, 2021 > -> [arXiv 2105.13440][https://arxiv.org/abs/2105.13440] +> [arXiv 2105.13440](https://arxiv.org/abs/2105.13440) If you use the **de_analysis** function in fastTopics, please cite: -> Interpreting structure in sequence count data with differential -> expression analysis allowing for grades of membership. +> *Interpreting structure in sequence count data with differential +> expression analysis allowing for grades of membership.* > > P. Carbonetto, K. Luo, A. Sarkar, A. Hung, K. Tayeb, S. Pott and > M. Stephens > > bioRxiv, 2023. > -> doi: [10.1101/2023.03.03.531029][https://doi.org/10.1101/2023.03.03.531029] +> doi: [10.1101/2023.03.03.531029](https://doi.org/10.1101/2023.03.03.531029) -To begin, loading the packages used to perform the analysis. +To begin, load the packages we will need to perform the analysis. ```{r load-pkgs, message=FALSE, warning=FALSE} library(Seurat) @@ -68,13 +68,13 @@ library(fastTopics) library(cowplot) ``` -And set the seed so that the results can be reproduced. +Set the seed so that the results can be reproduced. ```{r set-seed} set.seed(1) ``` -Load and, if necessary, install the PBMC 3k data set containing +Load (and install if necessary) the PBMC 3k data set containing transcription profiles for 2,700 cells. ```{r load-data, warning=FALSE} @@ -91,10 +91,10 @@ minutes to fit the model. pbmc3k <- FitTopicModel(pbmc3k,k = 6) ``` -To fit a topic model, we must specify $K$, the number of topics. -Here, we have chosen $K = 6$ topics. In most settings, a good choice -of $K$ will not be known in advance, so you will you want to explore -the results from topic models at different settings of $K$. +To fit a topic model, we had to choose $K$, the number of topics. +Here, we chose $K = 6$ topics. In most settings, a good choice of $K$ +will not be known in advance, so you will likely want to explore the +different settings of $K$. This first plot shows the cells projected onto the top two PCs of the topic proportions. @@ -105,7 +105,7 @@ DimPlot(pbmc3k,reduction = "pca_topics",pt.size = 1) + theme_cowplot(font_size = 10) ``` -Compare this against the top two PCs of the transformed counts: +Compare this with the top two PCs of the normalized and scaled counts: ```{r pca-2, fig.width=4.5, fig.height=3, message=FALSE} pbmc3k <- FindVariableFeatures(pbmc3k) @@ -123,16 +123,18 @@ in the "misc" slot: fit <- Misc(Reductions(pbmc3k,"multinom_topic_model")) ``` -Once the fitted topic model is extracted, many fastTopics functions -can be used for analysis and visualization. For example, the Structure -plot provides an evocative visual summary of the estimated topic -proportions for each cell. Here, we have grouped the cells by -previously inferred labels. +Once the topic model has been extracted from the Seurat object, many +fastTopics functions can be used for downstream analysis and +visualization. For example, we can create a *Structure plot* to +visualize all $K$ dimensions of the topic model simultaneously: ```{r structure-plot, fig.width=7.5, fig.height=1.75, results="hide", message=FALSE} structure_plot(fit,grouping = Idents(pbmc3k),gap = 25) ``` +In this plot, each position along the x-axis is a cell, and bar +heights correspond to topic proportions (which sum to 1 for each cell). + TO DO: Illustrate the use of the de_analysis. This is the version of R and the packages that were used to generate From d23470cb5decbfc5f3e18e36a16520353d6d84a4 Mon Sep 17 00:00:00 2001 From: Peter Carbonetto Date: Wed, 15 Mar 2023 20:24:23 -0500 Subject: [PATCH 33/50] Rebuilt the fasttopics vignette html. --- docs/fasttopics.html | 156 +++++++++++++++++++++---------------------- 1 file changed, 77 insertions(+), 79 deletions(-) diff --git a/docs/fasttopics.html b/docs/fasttopics.html index 970c643..7768d05 100644 --- a/docs/fasttopics.html +++ b/docs/fasttopics.html @@ -380,55 +380,56 @@

Compiled: March 15, 2023

of membership models.

K. K. Dey, C. Joyce Hsiao and M. Stephens.

PLoS Genetics, 2017.

-

doi: [10.1371/journal.pgen.1006599][countclust-paper]

+

doi: 10.1371/journal.pgen.1006599

and

-

Non-negative matrix factorization algorithms greatly improve topic -model fits.

+

Non-negative matrix factorization algorithms greatly improve +topic model fits.

P. Carbonetto, A. Sarkar, Z. Wang and M. Stephens.

arXiv, 2021

-

[arXiv 2105.13440][https://arxiv.org/abs/2105.13440]

+

arXiv 2105.13440

If you use the de_analysis function in fastTopics, please cite:

-

Interpreting structure in sequence count data with differential -expression analysis allowing for grades of membership.

+

Interpreting structure in sequence count data with differential +expression analysis allowing for grades of membership.

P. Carbonetto, K. Luo, A. Sarkar, A. Hung, K. Tayeb, S. Pott and M. Stephens

bioRxiv, 2023.

-

doi: [10.1101/2023.03.03.531029][https://doi.org/10.1101/2023.03.03.531029]

+

doi: 10.1101/2023.03.03.531029

-

To begin, loading the packages used to perform the analysis.

+

To begin, load the packages we will need to perform the analysis.

library(Seurat)
 library(SeuratData)
 library(SeuratWrappers)
 library(fastTopics)
 library(cowplot)
-

And set the seed so that the results can be reproduced.

+

Set the seed so that the results can be reproduced.

set.seed(1)
-

Load and, if necessary, install the PBMC 3k data set containing +

Load (and install if necessary) the PBMC 3k data set containing transcription profiles for 2,700 cells.

InstallData("pbmc3k")
 data(pbmc3k)
 dim(GetAssayData(pbmc3k))
 # [1] 13714  2700
-

Fit the multinomial topic model to the raw UMI counts. -No preprocessing or pre-selection of genes is needed. -It may take several minutes to fit the model.

+

Fit the multinomial topic model to the raw UMI counts. No data +preprocessing or gene filtering is needed. It may take several +minutes to fit the model.

pbmc3k <- FitTopicModel(pbmc3k, k = 6)
-

To fit a topic model, we must specify \(K\), the number of topics. Here, we have -chosen \(K = 6\) topics. In most -settings, a good choice of \(K\) will -not be known in advance, so you will you want to explore the results -from topic models at different settings of \(K\).

+

To fit a topic model, we had to choose \(K\), the number of topics. Here, we chose +\(K = 6\) topics. In most settings, a +good choice of \(K\) will not be known +in advance, so you will likely want to explore the different settings of +\(K\).

This first plot shows the cells projected onto the top two PCs of the topic proportions.

Idents(pbmc3k) <- pbmc3k$seurat_annotations
 DimPlot(pbmc3k, reduction = "pca_topics", pt.size = 1) + theme_cowplot(font_size = 10)

-

Compare this against the top two PCs of the transformed counts:

+

Compare this with the top two PCs of the normalized and scaled +counts:

pbmc3k <- FindVariableFeatures(pbmc3k)
 pbmc3k <- NormalizeData(pbmc3k)
 pbmc3k <- ScaleData(pbmc3k)
@@ -438,13 +439,16 @@ 
Compiled: March 15, 2023

 
The fitted topic model—a “multinom_topic_model” object—is stored in
 the “misc” slot:

 
fit <- Misc(Reductions(pbmc3k, "multinom_topic_model"))

-
Once the fitted topic model is extracted, many fastTopics functions
-can be used for analysis and visualization. For example, the Structure
-plot provides an evocative visual summary of the estimated mixture
-proportions for each cell. Here, we have grouped the cells by previously
-inferred labels.

+
Once the topic model has been extracted from the Seurat object, many
+fastTopics functions can be used for downstream analysis and
+visualization. For example, we can create a Structure plot to
+visualize all \(K\) dimensions of the
+topic model simultaneously:

 
structure_plot(fit, grouping = Idents(pbmc3k), gap = 25)

 

+
In this plot, each position along the x-axis is a cell, and bar
+heights correspond to topic proportions (which sum to 1 for each
+cell).

 
TO DO: Illustrate the use of the de_analysis.

 
This is the version of R and the packages that were used to generate
 these results:

@@ -468,63 +472,57 @@ 
Compiled: March 15, 2023

 # [1] stats     graphics  grDevices utils     datasets  methods   base     
 # 
 # other attached packages:
-# [1] rmarkdown_2.14          SeuratWrappers_0.3.1    pbmc3k.SeuratData_3.1.4
-# [4] cowplot_1.1.1           fastTopics_0.6-150      SeuratData_0.2.2       
-# [7] SeuratObject_4.1.3      Seurat_4.3.0           
+# [1] cowplot_1.1.1           fastTopics_0.6-150      SeuratWrappers_0.3.1   
+# [4] pbmc3k.SeuratData_3.1.4 SeuratData_0.2.2        SeuratObject_4.1.3     
+# [7] Seurat_4.3.0            rmarkdown_2.14         
 # 
 # loaded via a namespace (and not attached):
-#   [1] utf8_1.2.2             spatstat.explore_3.0-6 reticulate_1.24       
-#   [4] R.utils_2.11.0         tidyselect_1.1.2       htmlwidgets_1.5.4     
-#   [7] grid_4.2.0             Rtsne_0.16             devtools_2.4.3        
-#  [10] munsell_0.5.0          codetools_0.2-18       ica_1.0-2             
-#  [13] future_1.25.0          miniUI_0.1.1.1         withr_2.5.0           
-#  [16] spatstat.random_3.1-3  colorspace_2.0-3       progressr_0.10.0      
-#  [19] highr_0.9              knitr_1.39             rstudioapi_0.13       
-#  [22] ROCR_1.0-11            tensor_1.5             listenv_0.8.0         
-#  [25] labeling_0.4.2         mixsqp_0.3-48          polyclip_1.10-0       
-#  [28] MCMCpack_1.6-3         farver_2.1.0           rprojroot_2.0.3       
-#  [31] coda_0.19-4            parallelly_1.31.1      vctrs_0.4.1           
-#  [34] generics_0.1.2         xfun_0.30              R6_2.5.1              
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+# [1] plyr_1.8.7 igraph_1.3.1 lazyeval_0.2.2 +# [4] sp_1.6-0 splines_4.2.0 listenv_0.8.0 +# [7] scattermore_0.8 ggplot2_3.3.6 digest_0.6.29 +# [10] invgamma_1.1 htmltools_0.5.2 SQUAREM_2021.1 +# [13] fansi_1.0.3 magrittr_2.0.3 tensor_1.5 +# [16] cluster_2.1.3 ROCR_1.0-11 remotes_2.4.2 +# [19] globals_0.14.0 RcppParallel_5.1.5 matrixStats_0.62.0 +# [22] R.utils_2.11.0 MCMCpack_1.6-3 spatstat.sparse_3.0-0 +# [25] prettyunits_1.1.1 colorspace_2.0-3 rappdirs_0.3.3 +# [28] ggrepel_0.9.1 xfun_0.30 dplyr_1.0.9 +# [31] crayon_1.5.1 jsonlite_1.8.0 progressr_0.10.0 +# [34] spatstat.data_3.0-0 survival_3.3-1 zoo_1.8-10 +# [37] glue_1.6.2 polyclip_1.10-0 gtable_0.3.0 +# [40] MatrixModels_0.5-0 leiden_0.3.10 future.apply_1.9.0 +# [43] abind_1.4-5 SparseM_1.81 scales_1.2.0 +# [46] DBI_1.1.2 spatstat.random_3.1-3 miniUI_0.1.1.1 +# [49] Rcpp_1.0.9 progress_1.2.2 viridisLite_0.4.0 +# [52] xtable_1.8-4 reticulate_1.24 rsvd_1.0.5 +# [55] truncnorm_1.0-8 htmlwidgets_1.5.4 httr_1.4.2 +# [58] RColorBrewer_1.1-3 ellipsis_0.3.2 ica_1.0-2 +# [61] farver_2.1.0 pkgconfig_2.0.3 R.methodsS3_1.8.1 +# [64] sass_0.4.1 uwot_0.1.14 deldir_1.0-6 +# [67] utf8_1.2.2 labeling_0.4.2 tidyselect_1.1.2 +# [70] rlang_1.0.2 reshape2_1.4.4 later_1.3.0 +# [73] munsell_0.5.0 tools_4.2.0 cli_3.3.0 +# [76] generics_0.1.2 ggridges_0.5.3 evaluate_0.15 +# [79] stringr_1.4.0 fastmap_1.1.0 yaml_2.3.5 +# [82] goftest_1.2-3 mcmc_0.9-7 knitr_1.39 +# [85] fitdistrplus_1.1-8 purrr_0.3.4 RANN_2.6.1 +# [88] pbapply_1.5-0 future_1.25.0 nlme_3.1-157 +# [91] mime_0.12 quantreg_5.93 formatR_1.12 +# [94] R.oo_1.24.0 compiler_4.2.0 plotly_4.10.0 +# [97] png_0.1-7 spatstat.utils_3.0-1 tibble_3.1.7 +# [100] bslib_0.3.1 stringi_1.7.6 highr_0.9 +# [103] lattice_0.20-45 Matrix_1.5-3 vctrs_0.4.1 +# [106] pillar_1.7.0 lifecycle_1.0.1 BiocManager_1.30.20 +# [109] spatstat.geom_3.0-6 lmtest_0.9-40 jquerylib_0.1.4 +# [112] RcppAnnoy_0.0.19 data.table_1.14.4 irlba_2.3.5 +# [115] httpuv_1.6.5 patchwork_1.1.1 R6_2.5.1 +# [118] promises_1.2.0.1 KernSmooth_2.23-20 gridExtra_2.3 +# [121] parallelly_1.31.1 codetools_0.2-18 MASS_7.3-56 +# [124] assertthat_0.2.1 sctransform_0.3.5 hms_1.1.1 +# [127] parallel_4.2.0 quadprog_1.5-8 grid_4.2.0 +# [130] tidyr_1.2.0 coda_0.19-4 ashr_2.2-54 +# [133] Rtsne_0.16 mixsqp_0.3-48 spatstat.explore_3.0-6 +# [136] shiny_1.7.1 From 43dd380b10f3508b26c29f88ab82618c5a46fc6d Mon Sep 17 00:00:00 2001 From: Peter Carbonetto Date: Wed, 15 Mar 2023 20:28:31 -0500 Subject: [PATCH 34/50] Small fix to the fasttopics rmd. --- docs/fasttopics.Rmd | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/docs/fasttopics.Rmd b/docs/fasttopics.Rmd index ddf245f..5117e47 100644 --- a/docs/fasttopics.Rmd +++ b/docs/fasttopics.Rmd @@ -29,7 +29,7 @@ cite: > *Visualizing the structure of RNA-seq expression data using grade of > membership models.* > -> K. K. Dey, C. Joyce Hsiao and M. Stephens. +> K. K. Dey, C. J. Hsiao and M. Stephens. > > PLoS Genetics, 2017. > From 4bba11b3d13d93c3db1026dbcf2a33899767b30f Mon Sep 17 00:00:00 2001 From: Peter Carbonetto Date: Thu, 16 Mar 2023 12:45:49 -0500 Subject: [PATCH 35/50] Added some text to the vignette explaining the structure plot. --- docs/fasttopics.Rmd | 28 +++++++++++++++++++++++++--- 1 file changed, 25 insertions(+), 3 deletions(-) diff --git a/docs/fasttopics.Rmd b/docs/fasttopics.Rmd index 5117e47..454ae8d 100644 --- a/docs/fasttopics.Rmd +++ b/docs/fasttopics.Rmd @@ -125,15 +125,37 @@ fit <- Misc(Reductions(pbmc3k,"multinom_topic_model")) Once the topic model has been extracted from the Seurat object, many fastTopics functions can be used for downstream analysis and -visualization. For example, we can create a *Structure plot* to +visualization. For example, we can create a "Structure plot" to visualize all $K$ dimensions of the topic model simultaneously: ```{r structure-plot, fig.width=7.5, fig.height=1.75, results="hide", message=FALSE} structure_plot(fit,grouping = Idents(pbmc3k),gap = 25) ``` -In this plot, each position along the x-axis is a cell, and bar -heights correspond to topic proportions (which sum to 1 for each cell). +In the Structure plot, each position along the x-axis is a cell, and +bar heights correspond to topic proportions (which sum to 1 for each +cell). + +From this plot, we see that topic 3 (green) closely corresponds to B +cells. Topics 1 (red) and 4 (purple) also correspond well to the two +types of monocytes, but there is some overlap; the purple topic is +shared by both types of monocytes. + +Topic 5 (orange) corresponds well to natural killer (NK) cells, and +topic 2 (blue) appears to capture biological processes common to T +cells. The CD8+ T cells have characteristics of both NK cells and T +cells---these are T cells that sometimes become ``NK-like''---and this +is captured in the topic model by assigning membership to both topics +2 and 5. + +The other cell types (platelets, dendritic cells), perhaps because +they are less abundant and/or difficult to distinguish from the other +cell types, are represented as combinations of the topics for the +other cell types. + +Topic 6 is present in almost all cells to varying degrees, and +therefore its biological interpretation is not at all clear from the +cell labeling. TO DO: Illustrate the use of the de_analysis. 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and Markdown outputs: +# library(rmarkdown) +# render("fasttopics.Rmd") +# render("fasttopics.Rmd",output_format = md_document()) + ```{r knitr-opts, include=FALSE} knitr::opts_chunk$set(comment = "#",collapse = TRUE,results = "hold", fig.align = "center",dpi = 120,tidy = TRUE, diff --git a/docs/fasttopics.html b/docs/fasttopics.html index 7768d05..d6bf1af 100644 --- a/docs/fasttopics.html +++ b/docs/fasttopics.html @@ -13,49 +13,13 @@ Analyzing Seurat data using fastTopics - - + + - - - - + + + + - - - + + + ",d.insertBefore(c.lastChild,d.firstChild)}function d(){var a=t.elements;return"string"==typeof a?a.split(" "):a}function e(a,b){var c=t.elements;"string"!=typeof c&&(c=c.join(" ")),"string"!=typeof a&&(a=a.join(" ")),t.elements=c+" "+a,j(b)}function f(a){var b=s[a[q]];return b||(b={},r++,a[q]=r,s[r]=b),b}function g(a,c,d){if(c||(c=b),l)return c.createElement(a);d||(d=f(c));var e;return 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[ this[ j ] ] : [] ); + }, + + end: function() { + return this.prevObject || this.constructor(); + }, + + // For internal use only. + // Behaves like an Array's method, not like a jQuery method. + push: push, + sort: arr.sort, + splice: arr.splice +}; + +jQuery.extend = jQuery.fn.extend = function() { + var options, name, src, copy, copyIsArray, clone, + target = arguments[ 0 ] || {}, + i = 1, + length = arguments.length, + deep = false; + + // Handle a deep copy situation + if ( typeof target === "boolean" ) { + deep = target; + + // Skip the boolean and the target + target = arguments[ i ] || {}; + i++; + } + + // Handle case when target is a string or something (possible in deep copy) + if ( typeof target !== "object" && !isFunction( target ) ) { + target = {}; + } + + // Extend jQuery itself if only one argument is passed + if ( i === length ) { + target = this; + i--; + } + + for ( ; i < length; i++ ) { + + // Only deal with non-null/undefined values + if ( ( options = arguments[ i ] ) != null ) { + + // Extend the base object + for ( name in options ) { + copy = options[ name ]; + + // Prevent Object.prototype pollution + // Prevent never-ending loop + if ( name === "__proto__" || target === copy ) { + continue; + } + + // Recurse if we're merging plain objects or arrays + if ( deep && copy && ( jQuery.isPlainObject( copy ) || + ( copyIsArray = Array.isArray( copy ) ) ) ) { + src = target[ name ]; + + // Ensure proper type for the source value + if ( copyIsArray && !Array.isArray( src ) ) { + clone = []; + } else if ( !copyIsArray && !jQuery.isPlainObject( src ) ) { + clone = {}; + } else { + clone = src; + } + copyIsArray = false; + + // Never move original objects, clone them + target[ name ] = jQuery.extend( deep, clone, copy ); + + // Don't bring in undefined values + } else if ( copy !== undefined ) { + target[ name ] = copy; + } + } + } + } + + // Return the modified object + return target; +}; + +jQuery.extend( { + + // Unique for each copy of jQuery on the page + expando: "jQuery" + ( version + Math.random() ).replace( /\D/g, "" ), + + // Assume jQuery is ready without the ready module + isReady: true, + + error: function( msg ) { + throw new Error( msg ); + }, + + noop: function() {}, + + isPlainObject: function( obj ) { + var proto, Ctor; + + // Detect obvious negatives + // Use toString instead of jQuery.type to catch host objects + if ( !obj || toString.call( obj ) !== "[object Object]" ) { + return false; + } + + proto = getProto( obj ); + + // Objects with no prototype (e.g., `Object.create( null )`) are plain + if ( !proto ) { + return true; + } + + // Objects with prototype are plain iff they were constructed by a global Object function + Ctor = hasOwn.call( proto, "constructor" ) && proto.constructor; + return typeof Ctor === "function" && fnToString.call( Ctor ) === ObjectFunctionString; + }, + + isEmptyObject: function( obj ) { + var name; + + for ( name in obj ) { + return false; + } + return true; + }, + + // Evaluates a script in a provided context; falls back to the global one + // if not specified. + globalEval: function( code, options, doc ) { + DOMEval( code, { nonce: options && options.nonce }, doc ); + }, + + each: function( obj, callback ) { + var length, i = 0; 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+ }, + + grep: function( elems, callback, invert ) { + var callbackInverse, + matches = [], + i = 0, + length = elems.length, + callbackExpect = !invert; + + // Go through the array, only saving the items + // that pass the validator function + for ( ; i < length; i++ ) { + callbackInverse = !callback( elems[ i ], i ); + if ( callbackInverse !== callbackExpect ) { + matches.push( elems[ i ] ); + } + } + + return matches; + }, + + // arg is for internal usage only + map: function( elems, callback, arg ) { + var length, value, + i = 0, + ret = []; + + // Go through the array, translating each of the items to their new values + if ( isArrayLike( elems ) ) { + length = elems.length; + for ( ; i < length; i++ ) { + value = callback( elems[ i ], i, arg ); + + if ( value != null ) { + ret.push( value ); + } + } + + // Go through every key on the object, + } else { + for ( i in elems ) { + value = callback( elems[ i ], i, arg ); + + if ( value != null ) { + ret.push( value ); + } + } + } + + // Flatten any nested arrays + return flat( ret ); + }, + + // A global GUID counter for objects + guid: 1, + + // jQuery.support is not used in Core but other projects attach their + // properties to it so it needs to exist. + support: support +} ); + +if ( typeof Symbol === "function" ) { + jQuery.fn[ Symbol.iterator ] = arr[ Symbol.iterator ]; +} + +// Populate the class2type map +jQuery.each( "Boolean Number String Function Array Date RegExp Object Error Symbol".split( " " ), + function( _i, name ) { + class2type[ "[object " + name + "]" ] = name.toLowerCase(); + } ); + +function isArrayLike( obj ) { + + // Support: real iOS 8.2 only (not reproducible in simulator) + // `in` check used to prevent JIT error (gh-2145) + // hasOwn isn't used here due to false negatives + // regarding Nodelist length in IE + var length = !!obj && "length" in obj && obj.length, + type = toType( obj ); + + if ( isFunction( obj ) || isWindow( obj ) ) { + return false; + } + + return type === "array" || length === 0 || + typeof length === "number" && length > 0 && ( length - 1 ) in obj; +} +var Sizzle = +/*! + * Sizzle CSS Selector Engine v2.3.6 + * https://sizzlejs.com/ + * + * Copyright JS Foundation and other contributors + * Released under the MIT license + * https://js.foundation/ + * + * Date: 2021-02-16 + */ +( function( window ) { +var i, + support, + Expr, + getText, + isXML, + tokenize, + compile, + select, + outermostContext, + sortInput, + hasDuplicate, + + // Local document vars + setDocument, + document, + docElem, + documentIsHTML, + rbuggyQSA, + rbuggyMatches, + matches, + contains, + + // Instance-specific data + expando = "sizzle" + 1 * new Date(), + preferredDoc = window.document, + dirruns = 0, + done = 0, + classCache = createCache(), + tokenCache = createCache(), + compilerCache = createCache(), + nonnativeSelectorCache = createCache(), + sortOrder = function( a, b ) { + if ( a === b ) { + hasDuplicate = true; + } + return 0; + }, + + // Instance methods + hasOwn = ( {} ).hasOwnProperty, + arr = [], + pop = arr.pop, + pushNative = arr.push, + push = arr.push, + slice = arr.slice, + + // Use a stripped-down indexOf as it's faster than native + // https://jsperf.com/thor-indexof-vs-for/5 + indexOf = function( list, elem ) { + var i = 0, + len = list.length; + for ( ; i < len; i++ ) { + if ( list[ i ] === elem ) { + return i; + } + } + return -1; + }, + + booleans = "checked|selected|async|autofocus|autoplay|controls|defer|disabled|hidden|" + + "ismap|loop|multiple|open|readonly|required|scoped", + + // Regular expressions + + // http://www.w3.org/TR/css3-selectors/#whitespace + whitespace = "[\\x20\\t\\r\\n\\f]", + + // https://www.w3.org/TR/css-syntax-3/#ident-token-diagram + identifier = "(?:\\\\[\\da-fA-F]{1,6}" + whitespace + + "?|\\\\[^\\r\\n\\f]|[\\w-]|[^\0-\\x7f])+", + + // Attribute selectors: http://www.w3.org/TR/selectors/#attribute-selectors + attributes = "\\[" + whitespace + "*(" + identifier + ")(?:" + whitespace + + + // Operator (capture 2) + "*([*^$|!~]?=)" + whitespace + + + // "Attribute values must be CSS identifiers [capture 5] + // or strings [capture 3 or capture 4]" + "*(?:'((?:\\\\.|[^\\\\'])*)'|\"((?:\\\\.|[^\\\\\"])*)\"|(" + identifier + "))|)" + + whitespace + "*\\]", + + pseudos = ":(" + identifier + ")(?:\\((" + + + // To reduce the number of selectors needing tokenize in the preFilter, prefer arguments: + // 1. quoted (capture 3; capture 4 or capture 5) + "('((?:\\\\.|[^\\\\'])*)'|\"((?:\\\\.|[^\\\\\"])*)\")|" + + + // 2. simple (capture 6) + "((?:\\\\.|[^\\\\()[\\]]|" + attributes + ")*)|" + + + // 3. anything else (capture 2) + ".*" + + ")\\)|)", + + // Leading and non-escaped trailing whitespace, capturing some non-whitespace characters preceding the latter + rwhitespace = new RegExp( whitespace + "+", "g" ), + rtrim = new RegExp( "^" + whitespace + "+|((?:^|[^\\\\])(?:\\\\.)*)" + + whitespace + "+$", "g" ), + + rcomma = new RegExp( "^" + whitespace + "*," + whitespace + "*" ), + rcombinators = new RegExp( "^" + whitespace + "*([>+~]|" + whitespace + ")" + whitespace + + "*" ), + rdescend = new RegExp( whitespace + "|>" ), + + rpseudo = new RegExp( pseudos ), + ridentifier = new RegExp( "^" + identifier + "$" ), + + matchExpr = { + "ID": new RegExp( "^#(" + identifier + ")" ), + "CLASS": new RegExp( "^\\.(" + identifier + ")" ), + "TAG": new RegExp( "^(" + identifier + "|[*])" ), + "ATTR": new RegExp( "^" + attributes ), + "PSEUDO": new RegExp( "^" + pseudos ), + "CHILD": new RegExp( "^:(only|first|last|nth|nth-last)-(child|of-type)(?:\\(" + + whitespace + "*(even|odd|(([+-]|)(\\d*)n|)" + whitespace + "*(?:([+-]|)" + + whitespace + "*(\\d+)|))" + whitespace + "*\\)|)", "i" ), + "bool": new RegExp( "^(?:" + booleans + ")$", "i" ), + + // For use in libraries implementing .is() + // We use this for POS matching in `select` + "needsContext": new RegExp( "^" + whitespace + + "*[>+~]|:(even|odd|eq|gt|lt|nth|first|last)(?:\\(" + whitespace + + "*((?:-\\d)?\\d*)" + whitespace + "*\\)|)(?=[^-]|$)", "i" ) + }, + + rhtml = /HTML$/i, + rinputs = /^(?:input|select|textarea|button)$/i, + rheader = /^h\d$/i, + + rnative = /^[^{]+\{\s*\[native \w/, + + // Easily-parseable/retrievable ID or TAG or CLASS selectors + rquickExpr = /^(?:#([\w-]+)|(\w+)|\.([\w-]+))$/, + + rsibling = /[+~]/, + + // CSS escapes + // http://www.w3.org/TR/CSS21/syndata.html#escaped-characters + runescape = new RegExp( "\\\\[\\da-fA-F]{1,6}" + whitespace + "?|\\\\([^\\r\\n\\f])", "g" ), + funescape = function( escape, nonHex ) { + var high = "0x" + escape.slice( 1 ) - 0x10000; + + return nonHex ? + + // Strip the backslash prefix from a non-hex escape sequence + nonHex : + + // Replace a hexadecimal escape sequence with the encoded Unicode code point + // Support: IE <=11+ + // For values outside the Basic Multilingual Plane (BMP), manually construct a + // surrogate pair + high < 0 ? + String.fromCharCode( high + 0x10000 ) : + String.fromCharCode( high >> 10 | 0xD800, high & 0x3FF | 0xDC00 ); + }, + + // CSS string/identifier serialization + // https://drafts.csswg.org/cssom/#common-serializing-idioms + rcssescape = /([\0-\x1f\x7f]|^-?\d)|^-$|[^\0-\x1f\x7f-\uFFFF\w-]/g, + fcssescape = function( ch, asCodePoint ) { + if ( asCodePoint ) { + + // U+0000 NULL becomes U+FFFD REPLACEMENT CHARACTER + if ( ch === "\0" ) { + return "\uFFFD"; + } + + // Control characters and (dependent upon position) numbers get escaped as code points + return ch.slice( 0, -1 ) + "\\" + + ch.charCodeAt( ch.length - 1 ).toString( 16 ) + " "; + } + + // Other potentially-special ASCII characters get backslash-escaped + return "\\" + ch; + }, + + // Used for iframes + // See setDocument() + // Removing the function wrapper causes a "Permission Denied" + // error in IE + unloadHandler = function() { + setDocument(); + }, + + inDisabledFieldset = addCombinator( + function( elem ) { + return elem.disabled === true && elem.nodeName.toLowerCase() === "fieldset"; + }, + { dir: "parentNode", next: "legend" } + ); + +// Optimize for push.apply( _, NodeList ) +try { + push.apply( + ( arr = slice.call( preferredDoc.childNodes ) ), + preferredDoc.childNodes + ); + + // Support: Android<4.0 + // Detect silently failing push.apply + // eslint-disable-next-line no-unused-expressions + arr[ preferredDoc.childNodes.length ].nodeType; +} catch ( e ) { + push = { apply: arr.length ? + + // Leverage slice if possible + function( target, els ) { + pushNative.apply( target, slice.call( els ) ); + } : + + // Support: IE<9 + // Otherwise append directly + function( target, els ) { + var j = target.length, + i = 0; + + // Can't trust NodeList.length + while ( ( target[ j++ ] = els[ i++ ] ) ) {} + target.length = j - 1; + } + }; +} + +function Sizzle( selector, context, results, seed ) { + var m, i, elem, nid, match, groups, newSelector, + newContext = context && context.ownerDocument, + + // nodeType defaults to 9, since context defaults to document + nodeType = context ? context.nodeType : 9; + + results = results || []; + + // Return early from calls with invalid selector or context + if ( typeof selector !== "string" || !selector || + nodeType !== 1 && nodeType !== 9 && nodeType !== 11 ) { + + return results; + } + + // Try to shortcut find operations (as opposed to filters) in HTML documents + if ( !seed ) { + setDocument( context ); + context = context || document; + + if ( documentIsHTML ) { + + // If the selector is sufficiently simple, try using a "get*By*" DOM method + // (excepting DocumentFragment context, where the methods don't exist) + if ( nodeType !== 11 && ( match = rquickExpr.exec( selector ) ) ) { + + // ID selector + if ( ( m = match[ 1 ] ) ) { + + // Document context + if ( nodeType === 9 ) { + if ( ( elem = context.getElementById( m ) ) ) { + + // Support: IE, Opera, Webkit + // TODO: identify versions + // getElementById can match elements by name instead of ID + if ( elem.id === m ) { + results.push( elem ); + return results; + } + } else { + return results; + } + + // Element context + } else { + + // Support: IE, Opera, Webkit + // TODO: identify versions + // getElementById can match elements by name instead of ID + if ( newContext && ( elem = newContext.getElementById( m ) ) && + contains( context, elem ) && + elem.id === m ) { + + results.push( elem ); + return results; + } + } + + // Type selector + } else if ( match[ 2 ] ) { + push.apply( results, context.getElementsByTagName( selector ) ); + return results; + + // Class selector + } else if ( ( m = match[ 3 ] ) && support.getElementsByClassName && + context.getElementsByClassName ) { + + push.apply( results, context.getElementsByClassName( m ) ); + return results; + } + } + + // Take advantage of querySelectorAll + if ( support.qsa && + !nonnativeSelectorCache[ selector + " " ] && + ( !rbuggyQSA || !rbuggyQSA.test( selector ) ) && + + // Support: IE 8 only + // Exclude object elements + ( nodeType !== 1 || context.nodeName.toLowerCase() !== "object" ) ) { + + newSelector = selector; + newContext = context; + + // qSA considers elements outside a scoping root when evaluating child or + // descendant combinators, which is not what we want. + // In such cases, we work around the behavior by prefixing every selector in the + // list with an ID selector referencing the scope context. + // The technique has to be used as well when a leading combinator is used + // as such selectors are not recognized by querySelectorAll. + // Thanks to Andrew Dupont for this technique. + if ( nodeType === 1 && + ( rdescend.test( selector ) || rcombinators.test( selector ) ) ) { + + // Expand context for sibling selectors + newContext = rsibling.test( selector ) && testContext( context.parentNode ) || + context; + + // We can use :scope instead of the ID hack if the browser + // supports it & if we're not changing the context. + if ( newContext !== context || !support.scope ) { + + // Capture the context ID, setting it first if necessary + if ( ( nid = context.getAttribute( "id" ) ) ) { + nid = nid.replace( rcssescape, fcssescape ); + } else { + context.setAttribute( "id", ( nid = expando ) ); + } + } + + // Prefix every selector in the list + groups = tokenize( selector ); + i = groups.length; + while ( i-- ) { + groups[ i ] = ( nid ? "#" + nid : ":scope" ) + " " + + toSelector( groups[ i ] ); + } + newSelector = groups.join( "," ); + } + + try { + push.apply( results, + newContext.querySelectorAll( newSelector ) + ); + return results; + } catch ( qsaError ) { + nonnativeSelectorCache( selector, true ); + } finally { + if ( nid === expando ) { + context.removeAttribute( "id" ); + } + } + } + } + } + + // All others + return select( selector.replace( rtrim, "$1" ), context, results, seed ); +} + +/** + * Create key-value caches of limited size + * @returns {function(string, object)} Returns the Object data after storing it on itself with + * property name the (space-suffixed) string and (if the cache is larger than Expr.cacheLength) + * deleting the oldest entry + */ +function createCache() { + var keys = []; + + function cache( key, value ) { + + // Use (key + " ") to avoid collision with native prototype properties (see Issue #157) + if ( keys.push( key + " " ) > Expr.cacheLength ) { + + // Only keep the most recent entries + delete cache[ keys.shift() ]; + } + return ( cache[ key + " " ] = value ); + } + return cache; +} + +/** + * Mark a function for special use by Sizzle + * @param {Function} fn The function to mark + */ +function markFunction( fn ) { + fn[ expando ] = true; + return fn; +} + +/** + * Support testing using an element + * @param {Function} fn Passed the created element and returns a boolean result + */ +function assert( fn ) { + var el = document.createElement( "fieldset" ); + + try { + return !!fn( el ); + } catch ( e ) { + return false; + } finally { + + // Remove from its parent by default + if ( el.parentNode ) { + el.parentNode.removeChild( el ); + } + + // release memory in IE + el = null; + } +} + +/** + * Adds the same handler for all of the specified attrs + * @param {String} attrs Pipe-separated list of attributes + * @param {Function} handler The method that will be applied + */ +function addHandle( attrs, handler ) { + var arr = attrs.split( "|" ), + i = arr.length; + + while ( i-- ) { + Expr.attrHandle[ arr[ i ] ] = handler; + } +} + +/** + * Checks document order of two siblings + * @param {Element} a + * @param {Element} b + * @returns {Number} Returns less than 0 if a precedes b, greater than 0 if a follows b + */ +function siblingCheck( a, b ) { + var cur = b && a, + diff = cur && a.nodeType === 1 && b.nodeType === 1 && + a.sourceIndex - b.sourceIndex; + + // Use IE sourceIndex if available on both nodes + if ( diff ) { + return diff; + } + + // Check if b follows a + if ( cur ) { + while ( ( cur = cur.nextSibling ) ) { + if ( cur === b ) { + return -1; + } + } + } + + return a ? 1 : -1; +} + +/** + * Returns a function to use in pseudos for input types + * @param {String} type + */ +function createInputPseudo( type ) { + return function( elem ) { + var name = elem.nodeName.toLowerCase(); + return name === "input" && elem.type === type; + }; +} + +/** + * Returns a function to use in pseudos for buttons + * @param {String} type + */ +function createButtonPseudo( type ) { + return function( elem ) { + var name = elem.nodeName.toLowerCase(); + return ( name === "input" || name === "button" ) && elem.type === type; + }; +} + +/** + * Returns a function to use in pseudos for :enabled/:disabled + * @param {Boolean} disabled true for :disabled; false for :enabled + */ +function createDisabledPseudo( disabled ) { + + // Known :disabled false positives: fieldset[disabled] > legend:nth-of-type(n+2) :can-disable + return function( elem ) { + + // Only certain elements can match :enabled or :disabled + // https://html.spec.whatwg.org/multipage/scripting.html#selector-enabled + // https://html.spec.whatwg.org/multipage/scripting.html#selector-disabled + if ( "form" in elem ) { + + // Check for inherited disabledness on relevant non-disabled elements: + // * listed form-associated elements in a disabled fieldset + // https://html.spec.whatwg.org/multipage/forms.html#category-listed + // https://html.spec.whatwg.org/multipage/forms.html#concept-fe-disabled + // * option elements in a disabled optgroup + // https://html.spec.whatwg.org/multipage/forms.html#concept-option-disabled + // All such elements have a "form" property. + if ( elem.parentNode && elem.disabled === false ) { + + // Option elements defer to a parent optgroup if present + if ( "label" in elem ) { + if ( "label" in elem.parentNode ) { + return elem.parentNode.disabled === disabled; + } else { + return elem.disabled === disabled; + } + } + + // Support: IE 6 - 11 + // Use the isDisabled shortcut property to check for disabled fieldset ancestors + return elem.isDisabled === disabled || + + // Where there is no isDisabled, check manually + /* jshint -W018 */ + elem.isDisabled !== !disabled && + inDisabledFieldset( elem ) === disabled; + } + + return elem.disabled === disabled; + + // Try to winnow out elements that can't be disabled before trusting the disabled property. + // Some victims get caught in our net (label, legend, menu, track), but it shouldn't + // even exist on them, let alone have a boolean value. + } else if ( "label" in elem ) { + return elem.disabled === disabled; + } + + // Remaining elements are neither :enabled nor :disabled + return false; + }; +} + +/** + * Returns a function to use in pseudos for positionals + * @param {Function} fn + */ +function createPositionalPseudo( fn ) { + return markFunction( function( argument ) { + argument = +argument; + return markFunction( function( seed, matches ) { + var j, + matchIndexes = fn( [], seed.length, argument ), + i = matchIndexes.length; + + // Match elements found at the specified indexes + while ( i-- ) { + if ( seed[ ( j = matchIndexes[ i ] ) ] ) { + seed[ j ] = !( matches[ j ] = seed[ j ] ); + } + } + } ); + } ); +} + +/** + * Checks a node for validity as a Sizzle context + * @param {Element|Object=} context + * @returns {Element|Object|Boolean} The input node if acceptable, otherwise a falsy value + */ +function testContext( context ) { + return context && typeof context.getElementsByTagName !== "undefined" && context; +} + +// Expose support vars for convenience +support = Sizzle.support = {}; + +/** + * Detects XML nodes + * @param {Element|Object} elem An element or a document + * @returns {Boolean} True iff elem is a non-HTML XML node + */ +isXML = Sizzle.isXML = function( elem ) { + var namespace = elem && elem.namespaceURI, + docElem = elem && ( elem.ownerDocument || elem ).documentElement; + + // Support: IE <=8 + // Assume HTML when documentElement doesn't yet exist, such as inside loading iframes + // https://bugs.jquery.com/ticket/4833 + return !rhtml.test( namespace || docElem && docElem.nodeName || "HTML" ); +}; + +/** + * Sets document-related variables once based on the current document + * @param {Element|Object} [doc] An element or document object to use to set the document + * @returns {Object} Returns the current document + */ +setDocument = Sizzle.setDocument = function( node ) { + var hasCompare, subWindow, + doc = node ? node.ownerDocument || node : preferredDoc; + + // Return early if doc is invalid or already selected + // Support: IE 11+, Edge 17 - 18+ + // IE/Edge sometimes throw a "Permission denied" error when strict-comparing + // two documents; shallow comparisons work. + // eslint-disable-next-line eqeqeq + if ( doc == document || doc.nodeType !== 9 || !doc.documentElement ) { + return document; + } + + // Update global variables + document = doc; + docElem = document.documentElement; + documentIsHTML = !isXML( document ); + + // Support: IE 9 - 11+, Edge 12 - 18+ + // Accessing iframe documents after unload throws "permission denied" errors (jQuery #13936) + // Support: IE 11+, Edge 17 - 18+ + // IE/Edge sometimes throw a "Permission denied" error when strict-comparing + // two documents; shallow comparisons work. + // eslint-disable-next-line eqeqeq + if ( preferredDoc != document && + ( subWindow = document.defaultView ) && subWindow.top !== subWindow ) { + + // Support: IE 11, Edge + if ( subWindow.addEventListener ) { + subWindow.addEventListener( "unload", unloadHandler, false ); + + // Support: IE 9 - 10 only + } else if ( subWindow.attachEvent ) { + subWindow.attachEvent( "onunload", unloadHandler ); + } + } + + // Support: IE 8 - 11+, Edge 12 - 18+, Chrome <=16 - 25 only, Firefox <=3.6 - 31 only, + // Safari 4 - 5 only, Opera <=11.6 - 12.x only + // IE/Edge & older browsers don't support the :scope pseudo-class. + // Support: Safari 6.0 only + // Safari 6.0 supports :scope but it's an alias of :root there. + support.scope = assert( function( el ) { + docElem.appendChild( el ).appendChild( document.createElement( "div" ) ); + return typeof el.querySelectorAll !== "undefined" && + !el.querySelectorAll( ":scope fieldset div" ).length; + } ); + + /* Attributes + ---------------------------------------------------------------------- */ + + // Support: IE<8 + // Verify that getAttribute really returns attributes and not properties + // (excepting IE8 booleans) + support.attributes = assert( function( el ) { + el.className = "i"; + return !el.getAttribute( "className" ); + } ); + + /* getElement(s)By* + ---------------------------------------------------------------------- */ + + // Check if getElementsByTagName("*") returns only elements + support.getElementsByTagName = assert( function( el ) { + el.appendChild( document.createComment( "" ) ); + return !el.getElementsByTagName( "*" ).length; + } ); + + // Support: IE<9 + support.getElementsByClassName = rnative.test( document.getElementsByClassName ); + + // Support: IE<10 + // Check if getElementById returns elements by name + // The broken getElementById methods don't pick up programmatically-set names, + // so use a roundabout getElementsByName test + support.getById = assert( function( el ) { + docElem.appendChild( el ).id = expando; + return !document.getElementsByName || !document.getElementsByName( expando ).length; + } ); + + // ID filter and find + if ( support.getById ) { + Expr.filter[ "ID" ] = function( id ) { + var attrId = id.replace( runescape, funescape ); + return function( elem ) { + return elem.getAttribute( "id" ) === attrId; + }; + }; + Expr.find[ "ID" ] = function( id, context ) { + if ( typeof context.getElementById !== "undefined" && documentIsHTML ) { + var elem = context.getElementById( id ); + return elem ? [ elem ] : []; + } + }; + } else { + Expr.filter[ "ID" ] = function( id ) { + var attrId = id.replace( runescape, funescape ); + return function( elem ) { + var node = typeof elem.getAttributeNode !== "undefined" && + elem.getAttributeNode( "id" ); + return node && node.value === attrId; + }; + }; + + // Support: IE 6 - 7 only + // getElementById is not reliable as a find shortcut + Expr.find[ "ID" ] = function( id, context ) { + if ( typeof context.getElementById !== "undefined" && documentIsHTML ) { + var node, i, elems, + elem = context.getElementById( id ); + + if ( elem ) { + + // Verify the id attribute + node = elem.getAttributeNode( "id" ); + if ( node && node.value === id ) { + return [ elem ]; + } + + // Fall back on getElementsByName + elems = context.getElementsByName( id ); + i = 0; + while ( ( elem = elems[ i++ ] ) ) { + node = elem.getAttributeNode( "id" ); + if ( node && node.value === id ) { + return [ elem ]; + } + } + } + + return []; + } + }; + } + + // Tag + Expr.find[ "TAG" ] = support.getElementsByTagName ? + function( tag, context ) { + if ( typeof context.getElementsByTagName !== "undefined" ) { + return context.getElementsByTagName( tag ); + + // DocumentFragment nodes don't have gEBTN + } else if ( support.qsa ) { + return context.querySelectorAll( tag ); + } + } : + + function( tag, context ) { + var elem, + tmp = [], + i = 0, + + // By happy coincidence, a (broken) gEBTN appears on DocumentFragment nodes too + results = context.getElementsByTagName( tag ); + + // Filter out possible comments + if ( tag === "*" ) { + while ( ( elem = results[ i++ ] ) ) { + if ( elem.nodeType === 1 ) { + tmp.push( elem ); + } + } + + return tmp; + } + return results; + }; + + // Class + Expr.find[ "CLASS" ] = support.getElementsByClassName && function( className, context ) { + if ( typeof context.getElementsByClassName !== "undefined" && documentIsHTML ) { + return context.getElementsByClassName( className ); + } + }; + + /* QSA/matchesSelector + ---------------------------------------------------------------------- */ + + // QSA and matchesSelector support + + // matchesSelector(:active) reports false when true (IE9/Opera 11.5) + rbuggyMatches = []; + + // qSa(:focus) reports false when true (Chrome 21) + // We allow this because of a bug in IE8/9 that throws an error + // whenever `document.activeElement` is accessed on an iframe + // So, we allow :focus to pass through QSA all the time to avoid the IE error + // See https://bugs.jquery.com/ticket/13378 + rbuggyQSA = []; + + if ( ( support.qsa = rnative.test( document.querySelectorAll ) ) ) { + + // Build QSA regex + // Regex strategy adopted from Diego Perini + assert( function( el ) { + + var input; + + // Select is set to empty string on purpose + // This is to test IE's treatment of not explicitly + // setting a boolean content attribute, + // since its presence should be enough + // https://bugs.jquery.com/ticket/12359 + docElem.appendChild( el ).innerHTML = "" + + ""; + + // Support: IE8, Opera 11-12.16 + // Nothing should be selected when empty strings follow ^= or $= or *= + // The test attribute must be unknown in Opera but "safe" for WinRT + // https://msdn.microsoft.com/en-us/library/ie/hh465388.aspx#attribute_section + if ( el.querySelectorAll( "[msallowcapture^='']" ).length ) { + rbuggyQSA.push( "[*^$]=" + whitespace + "*(?:''|\"\")" ); + } + + // Support: IE8 + // Boolean attributes and "value" are not treated correctly + if ( !el.querySelectorAll( "[selected]" ).length ) { + rbuggyQSA.push( "\\[" + whitespace + "*(?:value|" + booleans + ")" ); + } + + // Support: Chrome<29, Android<4.4, Safari<7.0+, iOS<7.0+, PhantomJS<1.9.8+ + if ( !el.querySelectorAll( "[id~=" + expando + "-]" ).length ) { + rbuggyQSA.push( "~=" ); + } + + // Support: IE 11+, Edge 15 - 18+ + // IE 11/Edge don't find elements on a `[name='']` query in some cases. + // Adding a temporary attribute to the document before the selection works + // around the issue. + // Interestingly, IE 10 & older don't seem to have the issue. + input = document.createElement( "input" ); + input.setAttribute( "name", "" ); + el.appendChild( input ); + if ( !el.querySelectorAll( "[name='']" ).length ) { + rbuggyQSA.push( "\\[" + whitespace + "*name" + whitespace + "*=" + + whitespace + "*(?:''|\"\")" ); + } + + // Webkit/Opera - :checked should return selected option elements + // http://www.w3.org/TR/2011/REC-css3-selectors-20110929/#checked + // IE8 throws error here and will not see later tests + if ( !el.querySelectorAll( ":checked" ).length ) { + rbuggyQSA.push( ":checked" ); + } + + // Support: Safari 8+, iOS 8+ + // https://bugs.webkit.org/show_bug.cgi?id=136851 + // In-page `selector#id sibling-combinator selector` fails + if ( !el.querySelectorAll( "a#" + expando + "+*" ).length ) { + rbuggyQSA.push( ".#.+[+~]" ); + } + + // Support: Firefox <=3.6 - 5 only + // Old Firefox doesn't throw on a badly-escaped identifier. + el.querySelectorAll( "\\\f" ); + rbuggyQSA.push( "[\\r\\n\\f]" ); + } ); + + assert( function( el ) { + el.innerHTML = "" + + ""; + + // Support: Windows 8 Native Apps + // The type and name attributes are restricted during .innerHTML assignment + var input = document.createElement( "input" ); + input.setAttribute( "type", "hidden" ); + el.appendChild( input ).setAttribute( "name", "D" ); + + // Support: IE8 + // Enforce case-sensitivity of name attribute + if ( el.querySelectorAll( "[name=d]" ).length ) { + rbuggyQSA.push( "name" + whitespace + "*[*^$|!~]?=" ); + } + + // FF 3.5 - :enabled/:disabled and hidden elements (hidden elements are still enabled) + // IE8 throws error here and will not see later tests + if ( el.querySelectorAll( ":enabled" ).length !== 2 ) { + rbuggyQSA.push( ":enabled", ":disabled" ); + } + + // Support: IE9-11+ + // IE's :disabled selector does not pick up the children of disabled fieldsets + docElem.appendChild( el ).disabled = true; + if ( el.querySelectorAll( ":disabled" ).length !== 2 ) { + rbuggyQSA.push( ":enabled", ":disabled" ); + } + + // Support: Opera 10 - 11 only + // Opera 10-11 does not throw on post-comma invalid pseudos + el.querySelectorAll( "*,:x" ); + rbuggyQSA.push( ",.*:" ); + } ); + } + + if ( ( support.matchesSelector = rnative.test( ( matches = docElem.matches || + docElem.webkitMatchesSelector || + docElem.mozMatchesSelector || + docElem.oMatchesSelector || + docElem.msMatchesSelector ) ) ) ) { + + assert( function( el ) { + + // Check to see if it's possible to do matchesSelector + // on a disconnected node (IE 9) + support.disconnectedMatch = matches.call( el, "*" ); + + // This should fail with an exception + // Gecko does not error, returns false instead + matches.call( el, "[s!='']:x" ); + rbuggyMatches.push( "!=", pseudos ); + } ); + } + + rbuggyQSA = rbuggyQSA.length && new RegExp( rbuggyQSA.join( "|" ) ); + rbuggyMatches = rbuggyMatches.length && new RegExp( rbuggyMatches.join( "|" ) ); + + /* Contains + ---------------------------------------------------------------------- */ + hasCompare = rnative.test( docElem.compareDocumentPosition ); + + // Element contains another + // Purposefully self-exclusive + // As in, an element does not contain itself + contains = hasCompare || rnative.test( docElem.contains ) ? + function( a, b ) { + var adown = a.nodeType === 9 ? a.documentElement : a, + bup = b && b.parentNode; + return a === bup || !!( bup && bup.nodeType === 1 && ( + adown.contains ? + adown.contains( bup ) : + a.compareDocumentPosition && a.compareDocumentPosition( bup ) & 16 + ) ); + } : + function( a, b ) { + if ( b ) { + while ( ( b = b.parentNode ) ) { + if ( b === a ) { + return true; + } + } + } + return false; + }; + + /* Sorting + ---------------------------------------------------------------------- */ + + // Document order sorting + sortOrder = hasCompare ? + function( a, b ) { + + // Flag for duplicate removal + if ( a === b ) { + hasDuplicate = true; + return 0; + } + + // Sort on method existence if only one input has compareDocumentPosition + var compare = !a.compareDocumentPosition - !b.compareDocumentPosition; + if ( compare ) { + return compare; + } + + // Calculate position if both inputs belong to the same document + // Support: IE 11+, Edge 17 - 18+ + // IE/Edge sometimes throw a "Permission denied" error when strict-comparing + // two documents; shallow comparisons work. + // eslint-disable-next-line eqeqeq + compare = ( a.ownerDocument || a ) == ( b.ownerDocument || b ) ? + a.compareDocumentPosition( b ) : + + // Otherwise we know they are disconnected + 1; + + // Disconnected nodes + if ( compare & 1 || + ( !support.sortDetached && b.compareDocumentPosition( a ) === compare ) ) { + + // Choose the first element that is related to our preferred document + // Support: IE 11+, Edge 17 - 18+ + // IE/Edge sometimes throw a "Permission denied" error when strict-comparing + // two documents; shallow comparisons work. + // eslint-disable-next-line eqeqeq + if ( a == document || a.ownerDocument == preferredDoc && + contains( preferredDoc, a ) ) { + return -1; + } + + // Support: IE 11+, Edge 17 - 18+ + // IE/Edge sometimes throw a "Permission denied" error when strict-comparing + // two documents; shallow comparisons work. + // eslint-disable-next-line eqeqeq + if ( b == document || b.ownerDocument == preferredDoc && + contains( preferredDoc, b ) ) { + return 1; + } + + // Maintain original order + return sortInput ? + ( indexOf( sortInput, a ) - indexOf( sortInput, b ) ) : + 0; + } + + return compare & 4 ? -1 : 1; + } : + function( a, b ) { + + // Exit early if the nodes are identical + if ( a === b ) { + hasDuplicate = true; + return 0; + } + + var cur, + i = 0, + aup = a.parentNode, + bup = b.parentNode, + ap = [ a ], + bp = [ b ]; + + // Parentless nodes are either documents or disconnected + if ( !aup || !bup ) { + + // Support: IE 11+, Edge 17 - 18+ + // IE/Edge sometimes throw a "Permission denied" error when strict-comparing + // two documents; shallow comparisons work. + /* eslint-disable eqeqeq */ + return a == document ? -1 : + b == document ? 1 : + /* eslint-enable eqeqeq */ + aup ? -1 : + bup ? 1 : + sortInput ? + ( indexOf( sortInput, a ) - indexOf( sortInput, b ) ) : + 0; + + // If the nodes are siblings, we can do a quick check + } else if ( aup === bup ) { + return siblingCheck( a, b ); + } + + // Otherwise we need full lists of their ancestors for comparison + cur = a; + while ( ( cur = cur.parentNode ) ) { + ap.unshift( cur ); + } + cur = b; + while ( ( cur = cur.parentNode ) ) { + bp.unshift( cur ); + } + + // Walk down the tree looking for a discrepancy + while ( ap[ i ] === bp[ i ] ) { + i++; + } + + return i ? + + // Do a sibling check if the nodes have a common ancestor + siblingCheck( ap[ i ], bp[ i ] ) : + + // Otherwise nodes in our document sort first + // Support: IE 11+, Edge 17 - 18+ + // IE/Edge sometimes throw a "Permission denied" error when strict-comparing + // two documents; shallow comparisons work. + /* eslint-disable eqeqeq */ + ap[ i ] == preferredDoc ? -1 : + bp[ i ] == preferredDoc ? 1 : + /* eslint-enable eqeqeq */ + 0; + }; + + return document; +}; + +Sizzle.matches = function( expr, elements ) { + return Sizzle( expr, null, null, elements ); +}; + +Sizzle.matchesSelector = function( elem, expr ) { + setDocument( elem ); + + if ( support.matchesSelector && documentIsHTML && + !nonnativeSelectorCache[ expr + " " ] && + ( !rbuggyMatches || !rbuggyMatches.test( expr ) ) && + ( !rbuggyQSA || !rbuggyQSA.test( expr ) ) ) { + + try { + var ret = matches.call( elem, expr ); + + // IE 9's matchesSelector returns false on disconnected nodes + if ( ret || support.disconnectedMatch || + + // As well, disconnected nodes are said to be in a document + // fragment in IE 9 + elem.document && elem.document.nodeType !== 11 ) { + return ret; + } + } catch ( e ) { + nonnativeSelectorCache( expr, true ); + } + } + + return Sizzle( expr, document, null, [ elem ] ).length > 0; +}; + +Sizzle.contains = function( context, elem ) { + + // Set document vars if needed + // Support: IE 11+, Edge 17 - 18+ + // IE/Edge sometimes throw a "Permission denied" error when strict-comparing + // two documents; shallow comparisons work. + // eslint-disable-next-line eqeqeq + if ( ( context.ownerDocument || context ) != document ) { + setDocument( context ); + } + return contains( context, elem ); +}; + +Sizzle.attr = function( elem, name ) { + + // Set document vars if needed + // Support: IE 11+, Edge 17 - 18+ + // IE/Edge sometimes throw a "Permission denied" error when strict-comparing + // two documents; shallow comparisons work. + // eslint-disable-next-line eqeqeq + if ( ( elem.ownerDocument || elem ) != document ) { + setDocument( elem ); + } + + var fn = Expr.attrHandle[ name.toLowerCase() ], + + // Don't get fooled by Object.prototype properties (jQuery #13807) + val = fn && hasOwn.call( Expr.attrHandle, name.toLowerCase() ) ? + fn( elem, name, !documentIsHTML ) : + undefined; + + return val !== undefined ? + val : + support.attributes || !documentIsHTML ? + elem.getAttribute( name ) : + ( val = elem.getAttributeNode( name ) ) && val.specified ? + val.value : + null; +}; + +Sizzle.escape = function( sel ) { + return ( sel + "" ).replace( rcssescape, fcssescape ); +}; + +Sizzle.error = function( msg ) { + throw new Error( "Syntax error, unrecognized expression: " + msg ); +}; + +/** + * Document sorting and removing duplicates + * @param {ArrayLike} results + */ +Sizzle.uniqueSort = function( results ) { + var elem, + duplicates = [], + j = 0, + i = 0; + + // Unless we *know* we can detect duplicates, assume their presence + hasDuplicate = !support.detectDuplicates; + sortInput = !support.sortStable && results.slice( 0 ); + results.sort( sortOrder ); + + if ( hasDuplicate ) { + while ( ( elem = results[ i++ ] ) ) { + if ( elem === results[ i ] ) { + j = duplicates.push( i ); + } + } + while ( j-- ) { + results.splice( duplicates[ j ], 1 ); + } + } + + // Clear input after sorting to release objects + // See https://github.com/jquery/sizzle/pull/225 + sortInput = null; + + return results; +}; + +/** + * Utility function for retrieving the text value of an array of DOM nodes + * @param {Array|Element} elem + */ +getText = Sizzle.getText = function( elem ) { + var node, + ret = "", + i = 0, + nodeType = elem.nodeType; + + if ( !nodeType ) { + + // If no nodeType, this is expected to be an array + while ( ( node = elem[ i++ ] ) ) { + + // Do not traverse comment nodes + ret += getText( node ); + } + } else if ( nodeType === 1 || nodeType === 9 || nodeType === 11 ) { + + // Use textContent for elements + // innerText usage removed for consistency of new lines (jQuery #11153) + if ( typeof elem.textContent === "string" ) { + return elem.textContent; + } else { + + // Traverse its children + for ( elem = elem.firstChild; elem; elem = elem.nextSibling ) { + ret += getText( elem ); + } + } + } else if ( nodeType === 3 || nodeType === 4 ) { + return elem.nodeValue; + } + + // Do not include comment or processing instruction nodes + + return ret; +}; + +Expr = Sizzle.selectors = { + + // Can be adjusted by the user + cacheLength: 50, + + createPseudo: markFunction, + + match: matchExpr, + + attrHandle: {}, + + find: {}, + + relative: { + ">": { dir: "parentNode", first: true }, + " ": { dir: "parentNode" }, + "+": { dir: "previousSibling", first: true }, + "~": { dir: "previousSibling" } + }, + + preFilter: { + "ATTR": function( match ) { + match[ 1 ] = match[ 1 ].replace( runescape, funescape ); + + // Move the given value to match[3] whether quoted or unquoted + match[ 3 ] = ( match[ 3 ] || match[ 4 ] || + match[ 5 ] || "" ).replace( runescape, funescape ); + + if ( match[ 2 ] === "~=" ) { + match[ 3 ] = " " + match[ 3 ] + " "; + } + + return match.slice( 0, 4 ); + }, + + "CHILD": function( match ) { + + /* matches from matchExpr["CHILD"] + 1 type (only|nth|...) + 2 what (child|of-type) + 3 argument (even|odd|\d*|\d*n([+-]\d+)?|...) + 4 xn-component of xn+y argument ([+-]?\d*n|) + 5 sign of xn-component + 6 x of xn-component + 7 sign of y-component + 8 y of y-component + */ + match[ 1 ] = match[ 1 ].toLowerCase(); + + if ( match[ 1 ].slice( 0, 3 ) === "nth" ) { + + // nth-* requires argument + if ( !match[ 3 ] ) { + Sizzle.error( match[ 0 ] ); + } + + // numeric x and y parameters for Expr.filter.CHILD + // remember that false/true cast respectively to 0/1 + match[ 4 ] = +( match[ 4 ] ? + match[ 5 ] + ( match[ 6 ] || 1 ) : + 2 * ( match[ 3 ] === "even" || match[ 3 ] === "odd" ) ); + match[ 5 ] = +( ( match[ 7 ] + match[ 8 ] ) || match[ 3 ] === "odd" ); + + // other types prohibit arguments + } else if ( match[ 3 ] ) { + Sizzle.error( match[ 0 ] ); + } + + return match; + }, + + "PSEUDO": function( match ) { + var excess, + unquoted = !match[ 6 ] && match[ 2 ]; + + if ( matchExpr[ "CHILD" ].test( match[ 0 ] ) ) { + return null; + } + + // Accept quoted arguments as-is + if ( match[ 3 ] ) { + match[ 2 ] = match[ 4 ] || match[ 5 ] || ""; + + // Strip excess characters from unquoted arguments + } else if ( unquoted && rpseudo.test( unquoted ) && + + // Get excess from tokenize (recursively) + ( excess = tokenize( unquoted, true ) ) && + + // advance to the next closing parenthesis + ( excess = unquoted.indexOf( ")", unquoted.length - excess ) - unquoted.length ) ) { + + // excess is a negative index + match[ 0 ] = match[ 0 ].slice( 0, excess ); + match[ 2 ] = unquoted.slice( 0, excess ); + } + + // Return only captures needed by the pseudo filter method (type and argument) + return match.slice( 0, 3 ); + } + }, + + filter: { + + "TAG": function( nodeNameSelector ) { + var nodeName = nodeNameSelector.replace( runescape, funescape ).toLowerCase(); + return nodeNameSelector === "*" ? + function() { + return true; + } : + function( elem ) { + return elem.nodeName && elem.nodeName.toLowerCase() === nodeName; + }; + }, + + "CLASS": function( className ) { + var pattern = classCache[ className + " " ]; + + return pattern || + ( pattern = new RegExp( "(^|" + whitespace + + ")" + className + "(" + whitespace + "|$)" ) ) && classCache( + className, function( elem ) { + return pattern.test( + typeof elem.className === "string" && elem.className || + typeof elem.getAttribute !== "undefined" && + elem.getAttribute( "class" ) || + "" + ); + } ); + }, + + "ATTR": function( name, operator, check ) { + return function( elem ) { + var result = Sizzle.attr( elem, name ); + + if ( result == null ) { + return operator === "!="; + } + if ( !operator ) { + return true; + } + + result += ""; + + /* eslint-disable max-len */ + + return operator === "=" ? result === check : + operator === "!=" ? result !== check : + operator === "^=" ? check && result.indexOf( check ) === 0 : + operator === "*=" ? check && result.indexOf( check ) > -1 : + operator === "$=" ? check && result.slice( -check.length ) === check : + operator === "~=" ? ( " " + result.replace( rwhitespace, " " ) + " " ).indexOf( check ) > -1 : + operator === "|=" ? result === check || result.slice( 0, check.length + 1 ) === check + "-" : + false; + /* eslint-enable max-len */ + + }; + }, + + "CHILD": function( type, what, _argument, first, last ) { + var simple = type.slice( 0, 3 ) !== "nth", + forward = type.slice( -4 ) !== "last", + ofType = what === "of-type"; + + return first === 1 && last === 0 ? + + // Shortcut for :nth-*(n) + function( elem ) { + return !!elem.parentNode; + } : + + function( elem, _context, xml ) { + var cache, uniqueCache, outerCache, node, nodeIndex, start, + dir = simple !== forward ? "nextSibling" : "previousSibling", + parent = elem.parentNode, + name = ofType && elem.nodeName.toLowerCase(), + useCache = !xml && !ofType, + diff = false; + + if ( parent ) { + + // :(first|last|only)-(child|of-type) + if ( simple ) { + while ( dir ) { + node = elem; + while ( ( node = node[ dir ] ) ) { + if ( ofType ? + node.nodeName.toLowerCase() === name : + node.nodeType === 1 ) { + + return false; + } + } + + // Reverse direction for :only-* (if we haven't yet done so) + start = dir = type === "only" && !start && "nextSibling"; + } + return true; + } + + start = [ forward ? parent.firstChild : parent.lastChild ]; + + // non-xml :nth-child(...) stores cache data on `parent` + if ( forward && useCache ) { + + // Seek `elem` from a previously-cached index + + // ...in a gzip-friendly way + node = parent; + outerCache = node[ expando ] || ( node[ expando ] = {} ); + + // Support: IE <9 only + // Defend against cloned attroperties (jQuery gh-1709) + uniqueCache = outerCache[ node.uniqueID ] || + ( outerCache[ node.uniqueID ] = {} ); + + cache = uniqueCache[ type ] || []; + nodeIndex = cache[ 0 ] === dirruns && cache[ 1 ]; + diff = nodeIndex && cache[ 2 ]; + node = nodeIndex && parent.childNodes[ nodeIndex ]; + + while ( ( node = ++nodeIndex && node && node[ dir ] || + + // Fallback to seeking `elem` from the start + ( diff = nodeIndex = 0 ) || start.pop() ) ) { + + // When found, cache indexes on `parent` and break + if ( node.nodeType === 1 && ++diff && node === elem ) { + uniqueCache[ type ] = [ dirruns, nodeIndex, diff ]; + break; + } + } + + } else { + + // Use previously-cached element index if available + if ( useCache ) { + + // ...in a gzip-friendly way + node = elem; + outerCache = node[ expando ] || ( node[ expando ] = {} ); + + // Support: IE <9 only + // Defend against cloned attroperties (jQuery gh-1709) + uniqueCache = outerCache[ node.uniqueID ] || + ( outerCache[ node.uniqueID ] = {} ); + + cache = uniqueCache[ type ] || []; + nodeIndex = cache[ 0 ] === dirruns && cache[ 1 ]; + diff = nodeIndex; + } + + // xml :nth-child(...) + // or :nth-last-child(...) or :nth(-last)?-of-type(...) + if ( diff === false ) { + + // Use the same loop as above to seek `elem` from the start + while ( ( node = ++nodeIndex && node && node[ dir ] || + ( diff = nodeIndex = 0 ) || start.pop() ) ) { + + if ( ( ofType ? + node.nodeName.toLowerCase() === name : + node.nodeType === 1 ) && + ++diff ) { + + // Cache the index of each encountered element + if ( useCache ) { + outerCache = node[ expando ] || + ( node[ expando ] = {} ); + + // Support: IE <9 only + // Defend against cloned attroperties (jQuery gh-1709) + uniqueCache = outerCache[ node.uniqueID ] || + ( outerCache[ node.uniqueID ] = {} ); + + uniqueCache[ type ] = [ dirruns, diff ]; + } + + if ( node === elem ) { + break; + } + } + } + } + } + + // Incorporate the offset, then check against cycle size + diff -= last; + return diff === first || ( diff % first === 0 && diff / first >= 0 ); + } + }; + }, + + "PSEUDO": function( pseudo, argument ) { + + // pseudo-class names are case-insensitive + // http://www.w3.org/TR/selectors/#pseudo-classes + // Prioritize by case sensitivity in case custom pseudos are added with uppercase letters + // Remember that setFilters inherits from pseudos + var args, + fn = Expr.pseudos[ pseudo ] || Expr.setFilters[ pseudo.toLowerCase() ] || + Sizzle.error( "unsupported pseudo: " + pseudo ); + + // The user may use createPseudo to indicate that + // arguments are needed to create the filter function + // just as Sizzle does + if ( fn[ expando ] ) { + return fn( argument ); + } + + // But maintain support for old signatures + if ( fn.length > 1 ) { + args = [ pseudo, pseudo, "", argument ]; + return Expr.setFilters.hasOwnProperty( pseudo.toLowerCase() ) ? + markFunction( function( seed, matches ) { + var idx, + matched = fn( seed, argument ), + i = matched.length; + while ( i-- ) { + idx = indexOf( seed, matched[ i ] ); + seed[ idx ] = !( matches[ idx ] = matched[ i ] ); + } + } ) : + function( elem ) { + return fn( elem, 0, args ); + }; + } + + return fn; + } + }, + + pseudos: { + + // Potentially complex pseudos + "not": markFunction( function( selector ) { + + // Trim the selector passed to compile + // to avoid treating leading and trailing + // spaces as combinators + var input = [], + results = [], + matcher = compile( selector.replace( rtrim, "$1" ) ); + + return matcher[ expando ] ? + markFunction( function( seed, matches, _context, xml ) { + var elem, + unmatched = matcher( seed, null, xml, [] ), + i = seed.length; + + // Match elements unmatched by `matcher` + while ( i-- ) { + if ( ( elem = unmatched[ i ] ) ) { + seed[ i ] = !( matches[ i ] = elem ); + } + } + } ) : + function( elem, _context, xml ) { + input[ 0 ] = elem; + matcher( input, null, xml, results ); + + // Don't keep the element (issue #299) + input[ 0 ] = null; + return !results.pop(); + }; + } ), + + "has": markFunction( function( selector ) { + return function( elem ) { + return Sizzle( selector, elem ).length > 0; + }; + } ), + + "contains": markFunction( function( text ) { + text = text.replace( runescape, funescape ); + return function( elem ) { + return ( elem.textContent || getText( elem ) ).indexOf( text ) > -1; + }; + } ), + + // "Whether an element is represented by a :lang() selector + // is based solely on the element's language value + // being equal to the identifier C, + // or beginning with the identifier C immediately followed by "-". + // The matching of C against the element's language value is performed case-insensitively. + // The identifier C does not have to be a valid language name." + // http://www.w3.org/TR/selectors/#lang-pseudo + "lang": markFunction( function( lang ) { + + // lang value must be a valid identifier + if ( !ridentifier.test( lang || "" ) ) { + Sizzle.error( "unsupported lang: " + lang ); + } + lang = lang.replace( runescape, funescape ).toLowerCase(); + return function( elem ) { + var elemLang; + do { + if ( ( elemLang = documentIsHTML ? + elem.lang : + elem.getAttribute( "xml:lang" ) || elem.getAttribute( "lang" ) ) ) { + + elemLang = elemLang.toLowerCase(); + return elemLang === lang || elemLang.indexOf( lang + "-" ) === 0; + } + } while ( ( elem = elem.parentNode ) && elem.nodeType === 1 ); + return false; + }; + } ), + + // Miscellaneous + "target": function( elem ) { + var hash = window.location && window.location.hash; + return hash && hash.slice( 1 ) === elem.id; + }, + + "root": function( elem ) { + return elem === docElem; + }, + + "focus": function( elem ) { + return elem === document.activeElement && + ( !document.hasFocus || document.hasFocus() ) && + !!( elem.type || elem.href || ~elem.tabIndex ); + }, + + // Boolean properties + "enabled": createDisabledPseudo( false ), + "disabled": createDisabledPseudo( true ), + + "checked": function( elem ) { + + // In CSS3, :checked should return both checked and selected elements + // http://www.w3.org/TR/2011/REC-css3-selectors-20110929/#checked + var nodeName = elem.nodeName.toLowerCase(); + return ( nodeName === "input" && !!elem.checked ) || + ( nodeName === "option" && !!elem.selected ); + }, + + "selected": function( elem ) { + + // Accessing this property makes selected-by-default + // options in Safari work properly + if ( elem.parentNode ) { + // eslint-disable-next-line no-unused-expressions + elem.parentNode.selectedIndex; + } + + return elem.selected === true; + }, + + // Contents + "empty": function( elem ) { + + // http://www.w3.org/TR/selectors/#empty-pseudo + // :empty is negated by element (1) or content nodes (text: 3; cdata: 4; entity ref: 5), + // but not by others (comment: 8; processing instruction: 7; etc.) + // nodeType < 6 works because attributes (2) do not appear as children + for ( elem = elem.firstChild; elem; elem = elem.nextSibling ) { + if ( elem.nodeType < 6 ) { + return false; + } + } + return true; + }, + + "parent": function( elem ) { + return !Expr.pseudos[ "empty" ]( elem ); + }, + + // Element/input types + "header": function( elem ) { + return rheader.test( elem.nodeName ); + }, + + "input": function( elem ) { + return rinputs.test( elem.nodeName ); + }, + + "button": function( elem ) { + var name = elem.nodeName.toLowerCase(); + return name === "input" && elem.type === "button" || name === "button"; + }, + + "text": function( elem ) { + var attr; + return elem.nodeName.toLowerCase() === "input" && + elem.type === "text" && + + // Support: IE<8 + // New HTML5 attribute values (e.g., "search") appear with elem.type === "text" + ( ( attr = elem.getAttribute( "type" ) ) == null || + attr.toLowerCase() === "text" ); + }, + + // Position-in-collection + "first": createPositionalPseudo( function() { + return [ 0 ]; + } ), + + "last": createPositionalPseudo( function( _matchIndexes, length ) { + return [ length - 1 ]; + } ), + + "eq": createPositionalPseudo( function( _matchIndexes, length, argument ) { + return [ argument < 0 ? argument + length : argument ]; + } ), + + "even": createPositionalPseudo( function( matchIndexes, length ) { + var i = 0; + for ( ; i < length; i += 2 ) { + matchIndexes.push( i ); + } + return matchIndexes; + } ), + + "odd": createPositionalPseudo( function( matchIndexes, length ) { + var i = 1; + for ( ; i < length; i += 2 ) { + matchIndexes.push( i ); + } + return matchIndexes; + } ), + + "lt": createPositionalPseudo( function( matchIndexes, length, argument ) { + var i = argument < 0 ? + argument + length : + argument > length ? + length : + argument; + for ( ; --i >= 0; ) { + matchIndexes.push( i ); + } + return matchIndexes; + } ), + + "gt": createPositionalPseudo( function( matchIndexes, length, argument ) { + var i = argument < 0 ? argument + length : argument; + for ( ; ++i < length; ) { + matchIndexes.push( i ); + } + return matchIndexes; + } ) + } +}; + +Expr.pseudos[ "nth" ] = Expr.pseudos[ "eq" ]; + +// Add button/input type pseudos +for ( i in { radio: true, checkbox: true, file: true, password: true, image: true } ) { + Expr.pseudos[ i ] = createInputPseudo( i ); +} +for ( i in { submit: true, reset: true } ) { + Expr.pseudos[ i ] = createButtonPseudo( i ); +} + +// Easy API for creating new setFilters +function setFilters() {} +setFilters.prototype = Expr.filters = Expr.pseudos; +Expr.setFilters = new setFilters(); + +tokenize = Sizzle.tokenize = function( selector, parseOnly ) { + var matched, match, tokens, type, + soFar, groups, preFilters, + cached = tokenCache[ selector + " " ]; + + if ( cached ) { + return parseOnly ? 0 : cached.slice( 0 ); + } + + soFar = selector; + groups = []; + preFilters = Expr.preFilter; + + while ( soFar ) { + + // Comma and first run + if ( !matched || ( match = rcomma.exec( soFar ) ) ) { + if ( match ) { + + // Don't consume trailing commas as valid + soFar = soFar.slice( match[ 0 ].length ) || soFar; + } + groups.push( ( tokens = [] ) ); + } + + matched = false; + + // Combinators + if ( ( match = rcombinators.exec( soFar ) ) ) { + matched = match.shift(); + tokens.push( { + value: matched, + + // Cast descendant combinators to space + type: match[ 0 ].replace( rtrim, " " ) + } ); + soFar = soFar.slice( matched.length ); + } + + // Filters + for ( type in Expr.filter ) { + if ( ( match = matchExpr[ type ].exec( soFar ) ) && ( !preFilters[ type ] || + ( match = preFilters[ type ]( match ) ) ) ) { + matched = match.shift(); + tokens.push( { + value: matched, + type: type, + matches: match + } ); + soFar = soFar.slice( matched.length ); + } + } + + if ( !matched ) { + break; + } + } + + // Return the length of the invalid excess + // if we're just parsing + // Otherwise, throw an error or return tokens + return parseOnly ? + soFar.length : + soFar ? + Sizzle.error( selector ) : + + // Cache the tokens + tokenCache( selector, groups ).slice( 0 ); +}; + +function toSelector( tokens ) { + var i = 0, + len = tokens.length, + selector = ""; + for ( ; i < len; i++ ) { + selector += tokens[ i ].value; + } + return selector; +} + +function addCombinator( matcher, combinator, base ) { + var dir = combinator.dir, + skip = combinator.next, + key = skip || dir, + checkNonElements = base && key === "parentNode", + doneName = done++; + + return combinator.first ? + + // Check against closest ancestor/preceding element + function( elem, context, xml ) { + while ( ( elem = elem[ dir ] ) ) { + if ( elem.nodeType === 1 || checkNonElements ) { + return matcher( elem, context, xml ); + } + } + return false; + } : + + // Check against all ancestor/preceding elements + function( elem, context, xml ) { + var oldCache, uniqueCache, outerCache, + newCache = [ dirruns, doneName ]; + + // We can't set arbitrary data on XML nodes, so they don't benefit from combinator caching + if ( xml ) { + while ( ( elem = elem[ dir ] ) ) { + if ( elem.nodeType === 1 || checkNonElements ) { + if ( matcher( elem, context, xml ) ) { + return true; + } + } + } + } else { + while ( ( elem = elem[ dir ] ) ) { + if ( elem.nodeType === 1 || checkNonElements ) { + outerCache = elem[ expando ] || ( elem[ expando ] = {} ); + + // Support: IE <9 only + // Defend against cloned attroperties (jQuery gh-1709) + uniqueCache = outerCache[ elem.uniqueID ] || + ( outerCache[ elem.uniqueID ] = {} ); + + if ( skip && skip === elem.nodeName.toLowerCase() ) { + elem = elem[ dir ] || elem; + } else if ( ( oldCache = uniqueCache[ key ] ) && + oldCache[ 0 ] === dirruns && oldCache[ 1 ] === doneName ) { + + // Assign to newCache so results back-propagate to previous elements + return ( newCache[ 2 ] = oldCache[ 2 ] ); + } else { + + // Reuse newcache so results back-propagate to previous elements + uniqueCache[ key ] = newCache; + + // A match means we're done; a fail means we have to keep checking + if ( ( newCache[ 2 ] = matcher( elem, context, xml ) ) ) { + return true; + } + } + } + } + } + return false; + }; +} + +function elementMatcher( matchers ) { + return matchers.length > 1 ? + function( elem, context, xml ) { + var i = matchers.length; + while ( i-- ) { + if ( !matchers[ i ]( elem, context, xml ) ) { + return false; + } + } + return true; + } : + matchers[ 0 ]; +} + +function multipleContexts( selector, contexts, results ) { + var i = 0, + len = contexts.length; + for ( ; i < len; i++ ) { + Sizzle( selector, contexts[ i ], results ); + } + return results; +} + +function condense( unmatched, map, filter, context, xml ) { + var elem, + newUnmatched = [], + i = 0, + len = unmatched.length, + mapped = map != null; + + for ( ; i < len; i++ ) { + if ( ( elem = unmatched[ i ] ) ) { + if ( !filter || filter( elem, context, xml ) ) { + newUnmatched.push( elem ); + if ( mapped ) { + map.push( i ); + } + } + } + } + + return newUnmatched; +} + +function setMatcher( preFilter, selector, matcher, postFilter, postFinder, postSelector ) { + if ( postFilter && !postFilter[ expando ] ) { + postFilter = setMatcher( postFilter ); + } + if ( postFinder && !postFinder[ expando ] ) { + postFinder = setMatcher( postFinder, postSelector ); + } + return markFunction( function( seed, results, context, xml ) { + var temp, i, elem, + preMap = [], + postMap = [], + preexisting = results.length, + + // Get initial elements from seed or context + elems = seed || multipleContexts( + selector || "*", + context.nodeType ? [ context ] : context, + [] + ), + + // Prefilter to get matcher input, preserving a map for seed-results synchronization + matcherIn = preFilter && ( seed || !selector ) ? + condense( elems, preMap, preFilter, context, xml ) : + elems, + + matcherOut = matcher ? + + // If we have a postFinder, or filtered seed, or non-seed postFilter or preexisting results, + postFinder || ( seed ? preFilter : preexisting || postFilter ) ? + + // ...intermediate processing is necessary + [] : + + // ...otherwise use results directly + results : + matcherIn; + + // Find primary matches + if ( matcher ) { + matcher( matcherIn, matcherOut, context, xml ); + } + + // Apply postFilter + if ( postFilter ) { + temp = condense( matcherOut, postMap ); + postFilter( temp, [], context, xml ); + + // Un-match failing elements by moving them back to matcherIn + i = temp.length; + while ( i-- ) { + if ( ( elem = temp[ i ] ) ) { + matcherOut[ postMap[ i ] ] = !( matcherIn[ postMap[ i ] ] = elem ); + } + } + } + + if ( seed ) { + if ( postFinder || preFilter ) { + if ( postFinder ) { + + // Get the final matcherOut by condensing this intermediate into postFinder contexts + temp = []; + i = matcherOut.length; + while ( i-- ) { + if ( ( elem = matcherOut[ i ] ) ) { + + // Restore matcherIn since elem is not yet a final match + temp.push( ( matcherIn[ i ] = elem ) ); + } + } + postFinder( null, ( matcherOut = [] ), temp, xml ); + } + + // Move matched elements from seed to results to keep them synchronized + i = matcherOut.length; + while ( i-- ) { + if ( ( elem = matcherOut[ i ] ) && + ( temp = postFinder ? indexOf( seed, elem ) : preMap[ i ] ) > -1 ) { + + seed[ temp ] = !( results[ temp ] = elem ); + } + } + } + + // Add elements to results, through postFinder if defined + } else { + matcherOut = condense( + matcherOut === results ? + matcherOut.splice( preexisting, matcherOut.length ) : + matcherOut + ); + if ( postFinder ) { + postFinder( null, results, matcherOut, xml ); + } else { + push.apply( results, matcherOut ); + } + } + } ); +} + +function matcherFromTokens( tokens ) { + var checkContext, matcher, j, + len = tokens.length, + leadingRelative = Expr.relative[ tokens[ 0 ].type ], + implicitRelative = leadingRelative || Expr.relative[ " " ], + i = leadingRelative ? 1 : 0, + + // The foundational matcher ensures that elements are reachable from top-level context(s) + matchContext = addCombinator( function( elem ) { + return elem === checkContext; + }, implicitRelative, true ), + matchAnyContext = addCombinator( function( elem ) { + return indexOf( checkContext, elem ) > -1; + }, implicitRelative, true ), + matchers = [ function( elem, context, xml ) { + var ret = ( !leadingRelative && ( xml || context !== outermostContext ) ) || ( + ( checkContext = context ).nodeType ? + matchContext( elem, context, xml ) : + matchAnyContext( elem, context, xml ) ); + + // Avoid hanging onto element (issue #299) + checkContext = null; + return ret; + } ]; + + for ( ; i < len; i++ ) { + if ( ( matcher = Expr.relative[ tokens[ i ].type ] ) ) { + matchers = [ addCombinator( elementMatcher( matchers ), matcher ) ]; + } else { + matcher = Expr.filter[ tokens[ i ].type ].apply( null, tokens[ i ].matches ); + + // Return special upon seeing a positional matcher + if ( matcher[ expando ] ) { + + // Find the next relative operator (if any) for proper handling + j = ++i; + for ( ; j < len; j++ ) { + if ( Expr.relative[ tokens[ j ].type ] ) { + break; + } + } + return setMatcher( + i > 1 && elementMatcher( matchers ), + i > 1 && toSelector( + + // If the preceding token was a descendant combinator, insert an implicit any-element `*` + tokens + .slice( 0, i - 1 ) + .concat( { value: tokens[ i - 2 ].type === " " ? "*" : "" } ) + ).replace( rtrim, "$1" ), + matcher, + i < j && matcherFromTokens( tokens.slice( i, j ) ), + j < len && matcherFromTokens( ( tokens = tokens.slice( j ) ) ), + j < len && toSelector( tokens ) + ); + } + matchers.push( matcher ); + } + } + + return elementMatcher( matchers ); +} + +function matcherFromGroupMatchers( elementMatchers, setMatchers ) { + var bySet = setMatchers.length > 0, + byElement = elementMatchers.length > 0, + superMatcher = function( seed, context, xml, results, outermost ) { + var elem, j, matcher, + matchedCount = 0, + i = "0", + unmatched = seed && [], + setMatched = [], + contextBackup = outermostContext, + + // We must always have either seed elements or outermost context + elems = seed || byElement && Expr.find[ "TAG" ]( "*", outermost ), + + // Use integer dirruns iff this is the outermost matcher + dirrunsUnique = ( dirruns += contextBackup == null ? 1 : Math.random() || 0.1 ), + len = elems.length; + + if ( outermost ) { + + // Support: IE 11+, Edge 17 - 18+ + // IE/Edge sometimes throw a "Permission denied" error when strict-comparing + // two documents; shallow comparisons work. + // eslint-disable-next-line eqeqeq + outermostContext = context == document || context || outermost; + } + + // Add elements passing elementMatchers directly to results + // Support: IE<9, Safari + // Tolerate NodeList properties (IE: "length"; Safari: ) matching elements by id + for ( ; i !== len && ( elem = elems[ i ] ) != null; i++ ) { + if ( byElement && elem ) { + j = 0; + + // Support: IE 11+, Edge 17 - 18+ + // IE/Edge sometimes throw a "Permission denied" error when strict-comparing + // two documents; shallow comparisons work. + // eslint-disable-next-line eqeqeq + if ( !context && elem.ownerDocument != document ) { + setDocument( elem ); + xml = !documentIsHTML; + } + while ( ( matcher = elementMatchers[ j++ ] ) ) { + if ( matcher( elem, context || document, xml ) ) { + results.push( elem ); + break; + } + } + if ( outermost ) { + dirruns = dirrunsUnique; + } + } + + // Track unmatched elements for set filters + if ( bySet ) { + + // They will have gone through all possible matchers + if ( ( elem = !matcher && elem ) ) { + matchedCount--; + } + + // Lengthen the array for every element, matched or not + if ( seed ) { + unmatched.push( elem ); + } + } + } + + // `i` is now the count of elements visited above, and adding it to `matchedCount` + // makes the latter nonnegative. + matchedCount += i; + + // Apply set filters to unmatched elements + // NOTE: This can be skipped if there are no unmatched elements (i.e., `matchedCount` + // equals `i`), unless we didn't visit _any_ elements in the above loop because we have + // no element matchers and no seed. + // Incrementing an initially-string "0" `i` allows `i` to remain a string only in that + // case, which will result in a "00" `matchedCount` that differs from `i` but is also + // numerically zero. + if ( bySet && i !== matchedCount ) { + j = 0; + while ( ( matcher = setMatchers[ j++ ] ) ) { + matcher( unmatched, setMatched, context, xml ); + } + + if ( seed ) { + + // Reintegrate element matches to eliminate the need for sorting + if ( matchedCount > 0 ) { + while ( i-- ) { + if ( !( unmatched[ i ] || setMatched[ i ] ) ) { + setMatched[ i ] = pop.call( results ); + } + } + } + + // Discard index placeholder values to get only actual matches + setMatched = condense( setMatched ); + } + + // Add matches to results + push.apply( results, setMatched ); + + // Seedless set matches succeeding multiple successful matchers stipulate sorting + if ( outermost && !seed && setMatched.length > 0 && + ( matchedCount + setMatchers.length ) > 1 ) { + + Sizzle.uniqueSort( results ); + } + } + + // Override manipulation of globals by nested matchers + if ( outermost ) { + dirruns = dirrunsUnique; + outermostContext = contextBackup; + } + + return unmatched; + }; + + return bySet ? + markFunction( superMatcher ) : + superMatcher; +} + +compile = Sizzle.compile = function( selector, match /* Internal Use Only */ ) { + var i, + setMatchers = [], + elementMatchers = [], + cached = compilerCache[ selector + " " ]; + + if ( !cached ) { + + // Generate a function of recursive functions that can be used to check each element + if ( !match ) { + match = tokenize( selector ); + } + i = match.length; + while ( i-- ) { + cached = matcherFromTokens( match[ i ] ); + if ( cached[ expando ] ) { + setMatchers.push( cached ); + } else { + elementMatchers.push( cached ); + } + } + + // Cache the compiled function + cached = compilerCache( + selector, + matcherFromGroupMatchers( elementMatchers, setMatchers ) + ); + + // Save selector and tokenization + cached.selector = selector; + } + return cached; +}; + +/** + * A low-level selection function that works with Sizzle's compiled + * selector functions + * @param {String|Function} selector A selector or a pre-compiled + * selector function built with Sizzle.compile + * @param {Element} context + * @param {Array} [results] + * @param {Array} [seed] A set of elements to match against + */ +select = Sizzle.select = function( selector, context, results, seed ) { + var i, tokens, token, type, find, + compiled = typeof selector === "function" && selector, + match = !seed && tokenize( ( selector = compiled.selector || selector ) ); + + results = results || []; + + // Try to minimize operations if there is only one selector in the list and no seed + // (the latter of which guarantees us context) + if ( match.length === 1 ) { + + // Reduce context if the leading compound selector is an ID + tokens = match[ 0 ] = match[ 0 ].slice( 0 ); + if ( tokens.length > 2 && ( token = tokens[ 0 ] ).type === "ID" && + context.nodeType === 9 && documentIsHTML && Expr.relative[ tokens[ 1 ].type ] ) { + + context = ( Expr.find[ "ID" ]( token.matches[ 0 ] + .replace( runescape, funescape ), context ) || [] )[ 0 ]; + if ( !context ) { + return results; + + // Precompiled matchers will still verify ancestry, so step up a level + } else if ( compiled ) { + context = context.parentNode; + } + + selector = selector.slice( tokens.shift().value.length ); + } + + // Fetch a seed set for right-to-left matching + i = matchExpr[ "needsContext" ].test( selector ) ? 0 : tokens.length; + while ( i-- ) { + token = tokens[ i ]; + + // Abort if we hit a combinator + if ( Expr.relative[ ( type = token.type ) ] ) { + break; + } + if ( ( find = Expr.find[ type ] ) ) { + + // Search, expanding context for leading sibling combinators + if ( ( seed = find( + token.matches[ 0 ].replace( runescape, funescape ), + rsibling.test( tokens[ 0 ].type ) && testContext( context.parentNode ) || + context + ) ) ) { + + // If seed is empty or no tokens remain, we can return early + tokens.splice( i, 1 ); + selector = seed.length && toSelector( tokens ); + if ( !selector ) { + push.apply( results, seed ); + return results; + } + + break; + } + } + } + } + + // Compile and execute a filtering function if one is not provided + // Provide `match` to avoid retokenization if we modified the selector above + ( compiled || compile( selector, match ) )( + seed, + context, + !documentIsHTML, + results, + !context || rsibling.test( selector ) && testContext( context.parentNode ) || context + ); + return results; +}; + +// One-time assignments + +// Sort stability +support.sortStable = expando.split( "" ).sort( sortOrder ).join( "" ) === expando; + +// Support: Chrome 14-35+ +// Always assume duplicates if they aren't passed to the comparison function +support.detectDuplicates = !!hasDuplicate; + +// Initialize against the default document +setDocument(); + +// Support: Webkit<537.32 - Safari 6.0.3/Chrome 25 (fixed in Chrome 27) +// Detached nodes confoundingly follow *each other* +support.sortDetached = assert( function( el ) { + + // Should return 1, but returns 4 (following) + return el.compareDocumentPosition( document.createElement( "fieldset" ) ) & 1; +} ); + +// Support: IE<8 +// Prevent attribute/property "interpolation" +// https://msdn.microsoft.com/en-us/library/ms536429%28VS.85%29.aspx +if ( !assert( function( el ) { + el.innerHTML = ""; + return el.firstChild.getAttribute( "href" ) === "#"; +} ) ) { + addHandle( "type|href|height|width", function( elem, name, isXML ) { + if ( !isXML ) { + return elem.getAttribute( name, name.toLowerCase() === "type" ? 1 : 2 ); + } + } ); +} + +// Support: IE<9 +// Use defaultValue in place of getAttribute("value") +if ( !support.attributes || !assert( function( el ) { + el.innerHTML = ""; + el.firstChild.setAttribute( "value", "" ); + return el.firstChild.getAttribute( "value" ) === ""; +} ) ) { + addHandle( "value", function( elem, _name, isXML ) { + if ( !isXML && elem.nodeName.toLowerCase() === "input" ) { + return elem.defaultValue; + } + } ); +} + +// Support: IE<9 +// Use getAttributeNode to fetch booleans when getAttribute lies +if ( !assert( function( el ) { + return el.getAttribute( "disabled" ) == null; +} ) ) { + addHandle( booleans, function( elem, name, isXML ) { + var val; + if ( !isXML ) { + return elem[ name ] === true ? name.toLowerCase() : + ( val = elem.getAttributeNode( name ) ) && val.specified ? + val.value : + null; + } + } ); +} + +return Sizzle; + +} )( window ); + + + +jQuery.find = Sizzle; +jQuery.expr = Sizzle.selectors; + +// Deprecated +jQuery.expr[ ":" ] = jQuery.expr.pseudos; +jQuery.uniqueSort = jQuery.unique = Sizzle.uniqueSort; +jQuery.text = Sizzle.getText; +jQuery.isXMLDoc = Sizzle.isXML; +jQuery.contains = Sizzle.contains; +jQuery.escapeSelector = Sizzle.escape; + + + + +var dir = function( elem, dir, until ) { + var matched = [], + truncate = until !== undefined; + + while ( ( elem = elem[ dir ] ) && elem.nodeType !== 9 ) { + if ( elem.nodeType === 1 ) { + if ( truncate && jQuery( elem ).is( until ) ) { + break; + } + matched.push( elem ); + } + } + return matched; +}; + + +var siblings = function( n, elem ) { + var matched = []; + + for ( ; n; n = n.nextSibling ) { + if ( n.nodeType === 1 && n !== elem ) { + matched.push( n ); + } + } + + return matched; +}; + + +var rneedsContext = jQuery.expr.match.needsContext; + + + +function nodeName( elem, name ) { + + return elem.nodeName && elem.nodeName.toLowerCase() === name.toLowerCase(); + +} +var rsingleTag = ( /^<([a-z][^\/\0>:\x20\t\r\n\f]*)[\x20\t\r\n\f]*\/?>(?:<\/\1>|)$/i ); + + + +// Implement the identical functionality for filter and not +function winnow( elements, qualifier, not ) { + if ( isFunction( qualifier ) ) { + return jQuery.grep( elements, function( elem, i ) { + return !!qualifier.call( elem, i, elem ) !== not; + } ); + } + + // Single element + if ( qualifier.nodeType ) { + return jQuery.grep( elements, function( elem ) { + return ( elem === qualifier ) !== not; + } ); + } + + // Arraylike of elements (jQuery, arguments, Array) + if ( typeof qualifier !== "string" ) { + return jQuery.grep( elements, function( elem ) { + return ( indexOf.call( qualifier, elem ) > -1 ) !== not; + } ); + } + + // Filtered directly for both simple and complex selectors + return jQuery.filter( qualifier, elements, not ); +} + +jQuery.filter = function( expr, elems, not ) { + var elem = elems[ 0 ]; + + if ( not ) { + expr = ":not(" + expr + ")"; + } + + if ( elems.length === 1 && elem.nodeType === 1 ) { + return jQuery.find.matchesSelector( elem, expr ) ? [ elem ] : []; + } + + return jQuery.find.matches( expr, jQuery.grep( elems, function( elem ) { + return elem.nodeType === 1; + } ) ); +}; + +jQuery.fn.extend( { + find: function( selector ) { + var i, ret, + len = this.length, + self = this; + + if ( typeof selector !== "string" ) { + return this.pushStack( jQuery( selector ).filter( function() { + for ( i = 0; i < len; i++ ) { + if ( jQuery.contains( self[ i ], this ) ) { + return true; + } + } + } ) ); + } + + ret = this.pushStack( [] ); + + for ( i = 0; i < len; i++ ) { + jQuery.find( selector, self[ i ], ret ); + } + + return len > 1 ? jQuery.uniqueSort( ret ) : ret; + }, + filter: function( selector ) { + return this.pushStack( winnow( this, selector || [], false ) ); + }, + not: function( selector ) { + return this.pushStack( winnow( this, selector || [], true ) ); + }, + is: function( selector ) { + return !!winnow( + this, + + // If this is a positional/relative selector, check membership in the returned set + // so $("p:first").is("p:last") won't return true for a doc with two "p". + typeof selector === "string" && rneedsContext.test( selector ) ? + jQuery( selector ) : + selector || [], + false + ).length; + } +} ); + + +// Initialize a jQuery object + + +// A central reference to the root jQuery(document) +var rootjQuery, + + // A simple way to check for HTML strings + // Prioritize #id over to avoid XSS via location.hash (#9521) + // Strict HTML recognition (#11290: must start with <) + // Shortcut simple #id case for speed + rquickExpr = /^(?:\s*(<[\w\W]+>)[^>]*|#([\w-]+))$/, + + init = jQuery.fn.init = function( selector, context, root ) { + var match, elem; + + // HANDLE: $(""), $(null), $(undefined), $(false) + if ( !selector ) { + return this; + } + + // Method init() accepts an alternate rootjQuery + // so migrate can support jQuery.sub (gh-2101) + root = root || rootjQuery; + + // Handle HTML strings + if ( typeof selector === "string" ) { + if ( selector[ 0 ] === "<" && + selector[ selector.length - 1 ] === ">" && + selector.length >= 3 ) { + + // Assume that strings that start and end with <> are HTML and skip the regex check + match = [ null, selector, null ]; + + } else { + match = rquickExpr.exec( selector ); + } + + // Match html or make sure no context is specified for #id + if ( match && ( match[ 1 ] || !context ) ) { + + // HANDLE: $(html) -> $(array) + if ( match[ 1 ] ) { + context = context instanceof jQuery ? context[ 0 ] : context; + + // Option to run scripts is true for back-compat + // Intentionally let the error be thrown if parseHTML is not present + jQuery.merge( this, jQuery.parseHTML( + match[ 1 ], + context && context.nodeType ? context.ownerDocument || context : document, + true + ) ); + + // HANDLE: $(html, props) + if ( rsingleTag.test( match[ 1 ] ) && jQuery.isPlainObject( context ) ) { + for ( match in context ) { + + // Properties of context are called as methods if possible + if ( isFunction( this[ match ] ) ) { + this[ match ]( context[ match ] ); + + // ...and otherwise set as attributes + } else { + this.attr( match, context[ match ] ); + } + } + } + + return this; + + // HANDLE: $(#id) + } else { + elem = document.getElementById( match[ 2 ] ); + + if ( elem ) { + + // Inject the element directly into the jQuery object + this[ 0 ] = elem; + this.length = 1; + } + return this; + } + + // HANDLE: $(expr, $(...)) + } else if ( !context || context.jquery ) { + return ( context || root ).find( selector ); + + // HANDLE: $(expr, context) + // (which is just equivalent to: $(context).find(expr) + } else { + return this.constructor( context ).find( selector ); + } + + // HANDLE: $(DOMElement) + } else if ( selector.nodeType ) { + this[ 0 ] = selector; + this.length = 1; + return this; + + // HANDLE: $(function) + // Shortcut for document ready + } else if ( isFunction( selector ) ) { + return root.ready !== undefined ? + root.ready( selector ) : + + // Execute immediately if ready is not present + selector( jQuery ); + } + + return jQuery.makeArray( selector, this ); + }; + +// Give the init function the jQuery prototype for later instantiation +init.prototype = jQuery.fn; + +// Initialize central reference +rootjQuery = jQuery( document ); + + +var rparentsprev = /^(?:parents|prev(?:Until|All))/, + + // Methods guaranteed to produce a unique set when starting from a unique set + guaranteedUnique = { + children: true, + contents: true, + next: true, + prev: true + }; + +jQuery.fn.extend( { + has: function( target ) { + var targets = jQuery( target, this ), + l = targets.length; + + return this.filter( function() { + var i = 0; + for ( ; i < l; i++ ) { + if ( jQuery.contains( this, targets[ i ] ) ) { + return true; + } + } + } ); + }, + + closest: function( selectors, context ) { + var cur, + i = 0, + l = this.length, + matched = [], + targets = typeof selectors !== "string" && jQuery( selectors ); + + // Positional selectors never match, since there's no _selection_ context + if ( !rneedsContext.test( selectors ) ) { + for ( ; i < l; i++ ) { + for ( cur = this[ i ]; cur && cur !== context; cur = cur.parentNode ) { + + // Always skip document fragments + if ( cur.nodeType < 11 && ( targets ? + targets.index( cur ) > -1 : + + // Don't pass non-elements to Sizzle + cur.nodeType === 1 && + jQuery.find.matchesSelector( cur, selectors ) ) ) { + + matched.push( cur ); + break; + } + } + } + } + + return this.pushStack( matched.length > 1 ? jQuery.uniqueSort( matched ) : matched ); + }, + + // Determine the position of an element within the set + index: function( elem ) { + + // No argument, return index in parent + if ( !elem ) { + return ( this[ 0 ] && this[ 0 ].parentNode ) ? this.first().prevAll().length : -1; + } + + // Index in selector + if ( typeof elem === "string" ) { + return indexOf.call( jQuery( elem ), this[ 0 ] ); + } + + // Locate the position of the desired element + return indexOf.call( this, + + // If it receives a jQuery object, the first element is used + elem.jquery ? elem[ 0 ] : elem + ); + }, + + add: function( selector, context ) { + return this.pushStack( + jQuery.uniqueSort( + jQuery.merge( this.get(), jQuery( selector, context ) ) + ) + ); + }, + + addBack: function( selector ) { + return this.add( selector == null ? + this.prevObject : this.prevObject.filter( selector ) + ); + } +} ); + +function sibling( cur, dir ) { + while ( ( cur = cur[ dir ] ) && cur.nodeType !== 1 ) {} + return cur; +} + +jQuery.each( { + parent: function( elem ) { + var parent = elem.parentNode; + return parent && parent.nodeType !== 11 ? parent : null; + }, + parents: function( elem ) { + return dir( elem, "parentNode" ); + }, + parentsUntil: function( elem, _i, until ) { + return dir( elem, "parentNode", until ); + }, + next: function( elem ) { + return sibling( elem, "nextSibling" ); + }, + prev: function( elem ) { + return sibling( elem, "previousSibling" ); + }, + nextAll: function( elem ) { + return dir( elem, "nextSibling" ); + }, + prevAll: function( elem ) { + return dir( elem, "previousSibling" ); + }, + nextUntil: function( elem, _i, until ) { + return dir( elem, "nextSibling", until ); + }, + prevUntil: function( elem, _i, until ) { + return dir( elem, "previousSibling", until ); + }, + siblings: function( elem ) { + return siblings( ( elem.parentNode || {} ).firstChild, elem ); + }, + children: function( elem ) { + return siblings( elem.firstChild ); + }, + contents: function( elem ) { + if ( elem.contentDocument != null && + + // Support: IE 11+ + // elements with no `data` attribute has an object + // `contentDocument` with a `null` prototype. + getProto( elem.contentDocument ) ) { + + return elem.contentDocument; + } + + // Support: IE 9 - 11 only, iOS 7 only, Android Browser <=4.3 only + // Treat the template element as a regular one in browsers that + // don't support it. + if ( nodeName( elem, "template" ) ) { + elem = elem.content || elem; + } + + return jQuery.merge( [], elem.childNodes ); + } +}, function( name, fn ) { + jQuery.fn[ name ] = function( until, selector ) { + var matched = jQuery.map( this, fn, until ); + + if ( name.slice( -5 ) !== "Until" ) { + selector = until; + } + + if ( selector && typeof selector === "string" ) { + matched = jQuery.filter( selector, matched ); + } + + if ( this.length > 1 ) { + + // Remove duplicates + if ( !guaranteedUnique[ name ] ) { + jQuery.uniqueSort( matched ); + } + + // Reverse order for parents* and prev-derivatives + if ( rparentsprev.test( name ) ) { + matched.reverse(); + } + } + + return this.pushStack( matched ); + }; +} ); +var rnothtmlwhite = ( /[^\x20\t\r\n\f]+/g ); + + + +// Convert String-formatted options into Object-formatted ones +function createOptions( options ) { + var object = {}; + jQuery.each( options.match( rnothtmlwhite ) || [], function( _, flag ) { + object[ flag ] = true; + } ); + return object; +} + +/* + * Create a callback list using the following parameters: + * + * options: an optional list of space-separated options that will change how + * the callback list behaves or a more traditional option object + * + * By default a callback list will act like an event callback list and can be + * "fired" multiple times. + * + * Possible options: + * + * once: will ensure the callback list can only be fired once (like a Deferred) + * + * memory: will keep track of previous values and will call any callback added + * after the list has been fired right away with the latest "memorized" + * values (like a Deferred) + * + * unique: will ensure a callback can only be added once (no duplicate in the list) + * + * stopOnFalse: interrupt callings when a callback returns false + * + */ +jQuery.Callbacks = function( options ) { + + // Convert options from String-formatted to Object-formatted if needed + // (we check in cache first) + options = typeof options === "string" ? + createOptions( options ) : + jQuery.extend( {}, options ); + + var // Flag to know if list is currently firing + firing, + + // Last fire value for non-forgettable lists + memory, + + // Flag to know if list was already fired + fired, + + // Flag to prevent firing + locked, + + // Actual callback list + list = [], + + // Queue of execution data for repeatable lists + queue = [], + + // Index of currently firing callback (modified by add/remove as needed) + firingIndex = -1, + + // Fire callbacks + fire = function() { + + // Enforce single-firing + locked = locked || options.once; + + // Execute callbacks for all pending executions, + // respecting firingIndex overrides and runtime changes + fired = firing = true; + for ( ; queue.length; firingIndex = -1 ) { + memory = queue.shift(); + while ( ++firingIndex < list.length ) { + + // Run callback and check for early termination + if ( list[ firingIndex ].apply( memory[ 0 ], memory[ 1 ] ) === false && + options.stopOnFalse ) { + + // Jump to end and forget the data so .add doesn't re-fire + firingIndex = list.length; + memory = false; + } + } + } + + // Forget the data if we're done with it + if ( !options.memory ) { + memory = false; + } + + firing = false; + + // Clean up if we're done firing for good + if ( locked ) { + + // Keep an empty list if we have data for future add calls + if ( memory ) { + list = []; + + // Otherwise, this object is spent + } else { + list = ""; + } + } + }, + + // Actual Callbacks object + self = { + + // Add a callback or a collection of callbacks to the list + add: function() { + if ( list ) { + + // If we have memory from a past run, we should fire after adding + if ( memory && !firing ) { + firingIndex = list.length - 1; + queue.push( memory ); + } + + ( function add( args ) { + jQuery.each( args, function( _, arg ) { + if ( isFunction( arg ) ) { + if ( !options.unique || !self.has( arg ) ) { + list.push( arg ); + } + } else if ( arg && arg.length && toType( arg ) !== "string" ) { + + // Inspect recursively + add( arg ); + } + } ); + } )( arguments ); + + if ( memory && !firing ) { + fire(); + } + } + return this; + }, + + // Remove a callback from the list + remove: function() { + jQuery.each( arguments, function( _, arg ) { + var index; + while ( ( index = jQuery.inArray( arg, list, index ) ) > -1 ) { + list.splice( index, 1 ); + + // Handle firing indexes + if ( index <= firingIndex ) { + firingIndex--; + } + } + } ); + return this; + }, + + // Check if a given callback is in the list. + // If no argument is given, return whether or not list has callbacks attached. + has: function( fn ) { + return fn ? + jQuery.inArray( fn, list ) > -1 : + list.length > 0; + }, + + // Remove all callbacks from the list + empty: function() { + if ( list ) { + list = []; + } + return this; + }, + + // Disable .fire and .add + // Abort any current/pending executions + // Clear all callbacks and values + disable: function() { + locked = queue = []; + list = memory = ""; + return this; + }, + disabled: function() { + return !list; + }, + + // Disable .fire + // Also disable .add unless we have memory (since it would have no effect) + // Abort any pending executions + lock: function() { + locked = queue = []; + if ( !memory && !firing ) { + list = memory = ""; + } + return this; + }, + locked: function() { + return !!locked; + }, + + // Call all callbacks with the given context and arguments + fireWith: function( context, args ) { + if ( !locked ) { + args = args || []; + args = [ context, args.slice ? args.slice() : args ]; + queue.push( args ); + if ( !firing ) { + fire(); + } + } + return this; + }, + + // Call all the callbacks with the given arguments + fire: function() { + self.fireWith( this, arguments ); + return this; + }, + + // To know if the callbacks have already been called at least once + fired: function() { + return !!fired; + } + }; + + return self; +}; + + +function Identity( v ) { + return v; +} +function Thrower( ex ) { + throw ex; +} + +function adoptValue( value, resolve, reject, noValue ) { + var method; + + try { + + // Check for promise aspect first to privilege synchronous behavior + if ( value && isFunction( ( method = value.promise ) ) ) { + method.call( value ).done( resolve ).fail( reject ); + + // Other thenables + } else if ( value && isFunction( ( method = value.then ) ) ) { + method.call( value, resolve, reject ); + + // Other non-thenables + } else { + + // Control `resolve` arguments by letting Array#slice cast boolean `noValue` to integer: + // * false: [ value ].slice( 0 ) => resolve( value ) + // * true: [ value ].slice( 1 ) => resolve() + resolve.apply( undefined, [ value ].slice( noValue ) ); + } + + // For Promises/A+, convert exceptions into rejections + // Since jQuery.when doesn't unwrap thenables, we can skip the extra checks appearing in + // Deferred#then to conditionally suppress rejection. + } catch ( value ) { + + // Support: Android 4.0 only + // Strict mode functions invoked without .call/.apply get global-object context + reject.apply( undefined, [ value ] ); + } +} + +jQuery.extend( { + + Deferred: function( func ) { + var tuples = [ + + // action, add listener, callbacks, + // ... .then handlers, argument index, [final state] + [ "notify", "progress", jQuery.Callbacks( "memory" ), + jQuery.Callbacks( "memory" ), 2 ], + [ "resolve", "done", jQuery.Callbacks( "once memory" ), + jQuery.Callbacks( "once memory" ), 0, "resolved" ], + [ "reject", "fail", jQuery.Callbacks( "once memory" ), + jQuery.Callbacks( "once memory" ), 1, "rejected" ] + ], + state = "pending", + promise = { + state: function() { + return state; + }, + always: function() { + deferred.done( arguments ).fail( arguments ); + return this; + }, + "catch": function( fn ) { + return promise.then( null, fn ); + }, + + // Keep pipe for back-compat + pipe: function( /* fnDone, fnFail, fnProgress */ ) { + var fns = arguments; + + return jQuery.Deferred( function( newDefer ) { + jQuery.each( tuples, function( _i, tuple ) { + + // Map tuples (progress, done, fail) to arguments (done, fail, progress) + var fn = isFunction( fns[ tuple[ 4 ] ] ) && fns[ tuple[ 4 ] ]; + + // deferred.progress(function() { bind to newDefer or newDefer.notify }) + // deferred.done(function() { bind to newDefer or newDefer.resolve }) + // deferred.fail(function() { bind to newDefer or newDefer.reject }) + deferred[ tuple[ 1 ] ]( function() { + var returned = fn && fn.apply( this, arguments ); + if ( returned && isFunction( returned.promise ) ) { + returned.promise() + .progress( newDefer.notify ) + .done( newDefer.resolve ) + .fail( newDefer.reject ); + } else { + newDefer[ tuple[ 0 ] + "With" ]( + this, + fn ? [ returned ] : arguments + ); + } + } ); + } ); + fns = null; + } ).promise(); + }, + then: function( onFulfilled, onRejected, onProgress ) { + var maxDepth = 0; + function resolve( depth, deferred, handler, special ) { + return function() { + var that = this, + args = arguments, + mightThrow = function() { + var returned, then; + + // Support: Promises/A+ section 2.3.3.3.3 + // https://promisesaplus.com/#point-59 + // Ignore double-resolution attempts + if ( depth < maxDepth ) { + return; + } + + returned = handler.apply( that, args ); + + // Support: Promises/A+ section 2.3.1 + // https://promisesaplus.com/#point-48 + if ( returned === deferred.promise() ) { + throw new TypeError( "Thenable self-resolution" ); + } + + // Support: Promises/A+ sections 2.3.3.1, 3.5 + // https://promisesaplus.com/#point-54 + // https://promisesaplus.com/#point-75 + // Retrieve `then` only once + then = returned && + + // Support: Promises/A+ section 2.3.4 + // https://promisesaplus.com/#point-64 + // Only check objects and functions for thenability + ( typeof returned === "object" || + typeof returned === "function" ) && + returned.then; + + // Handle a returned thenable + if ( isFunction( then ) ) { + + // Special processors (notify) just wait for resolution + if ( special ) { + then.call( + returned, + resolve( maxDepth, deferred, Identity, special ), + resolve( maxDepth, deferred, Thrower, special ) + ); + + // Normal processors (resolve) also hook into progress + } else { + + // ...and disregard older resolution values + maxDepth++; + + then.call( + returned, + resolve( maxDepth, deferred, Identity, special ), + resolve( maxDepth, deferred, Thrower, special ), + resolve( maxDepth, deferred, Identity, + deferred.notifyWith ) + ); + } + + // Handle all other returned values + } else { + + // Only substitute handlers pass on context + // and multiple values (non-spec behavior) + if ( handler !== Identity ) { + that = undefined; + args = [ returned ]; + } + + // Process the value(s) + // Default process is resolve + ( special || deferred.resolveWith )( that, args ); + } + }, + + // Only normal processors (resolve) catch and reject exceptions + process = special ? + mightThrow : + function() { + try { + mightThrow(); + } catch ( e ) { + + if ( jQuery.Deferred.exceptionHook ) { + jQuery.Deferred.exceptionHook( e, + process.stackTrace ); + } + + // Support: Promises/A+ section 2.3.3.3.4.1 + // https://promisesaplus.com/#point-61 + // Ignore post-resolution exceptions + if ( depth + 1 >= maxDepth ) { + + // Only substitute handlers pass on context + // and multiple values (non-spec behavior) + if ( handler !== Thrower ) { + that = undefined; + args = [ e ]; + } + + deferred.rejectWith( that, args ); + } + } + }; + + // Support: Promises/A+ section 2.3.3.3.1 + // https://promisesaplus.com/#point-57 + // Re-resolve promises immediately to dodge false rejection from + // subsequent errors + if ( depth ) { + process(); + } else { + + // Call an optional hook to record the stack, in case of exception + // since it's otherwise lost when execution goes async + if ( jQuery.Deferred.getStackHook ) { + process.stackTrace = jQuery.Deferred.getStackHook(); + } + window.setTimeout( process ); + } + }; + } + + return jQuery.Deferred( function( newDefer ) { + + // progress_handlers.add( ... ) + tuples[ 0 ][ 3 ].add( + resolve( + 0, + newDefer, + isFunction( onProgress ) ? + onProgress : + Identity, + newDefer.notifyWith + ) + ); + + // fulfilled_handlers.add( ... ) + tuples[ 1 ][ 3 ].add( + resolve( + 0, + newDefer, + isFunction( onFulfilled ) ? + onFulfilled : + Identity + ) + ); + + // rejected_handlers.add( ... ) + tuples[ 2 ][ 3 ].add( + resolve( + 0, + newDefer, + isFunction( onRejected ) ? + onRejected : + Thrower + ) + ); + } ).promise(); + }, + + // Get a promise for this deferred + // If obj is provided, the promise aspect is added to the object + promise: function( obj ) { + return obj != null ? jQuery.extend( obj, promise ) : promise; + } + }, + deferred = {}; + + // Add list-specific methods + jQuery.each( tuples, function( i, tuple ) { + var list = tuple[ 2 ], + stateString = tuple[ 5 ]; + + // promise.progress = list.add + // promise.done = list.add + // promise.fail = list.add + promise[ tuple[ 1 ] ] = list.add; + + // Handle state + if ( stateString ) { + list.add( + function() { + + // state = "resolved" (i.e., fulfilled) + // state = "rejected" + state = stateString; + }, + + // rejected_callbacks.disable + // fulfilled_callbacks.disable + tuples[ 3 - i ][ 2 ].disable, + + // rejected_handlers.disable + // fulfilled_handlers.disable + tuples[ 3 - i ][ 3 ].disable, + + // progress_callbacks.lock + tuples[ 0 ][ 2 ].lock, + + // progress_handlers.lock + tuples[ 0 ][ 3 ].lock + ); + } + + // progress_handlers.fire + // fulfilled_handlers.fire + // rejected_handlers.fire + list.add( tuple[ 3 ].fire ); + + // deferred.notify = function() { deferred.notifyWith(...) } + // deferred.resolve = function() { deferred.resolveWith(...) } + // deferred.reject = function() { deferred.rejectWith(...) } + deferred[ tuple[ 0 ] ] = function() { + deferred[ tuple[ 0 ] + "With" ]( this === deferred ? undefined : this, arguments ); + return this; + }; + + // deferred.notifyWith = list.fireWith + // deferred.resolveWith = list.fireWith + // deferred.rejectWith = list.fireWith + deferred[ tuple[ 0 ] + "With" ] = list.fireWith; + } ); + + // Make the deferred a promise + promise.promise( deferred ); + + // Call given func if any + if ( func ) { + func.call( deferred, deferred ); + } + + // All done! + return deferred; + }, + + // Deferred helper + when: function( singleValue ) { + var + + // count of uncompleted subordinates + remaining = arguments.length, + + // count of unprocessed arguments + i = remaining, + + // subordinate fulfillment data + resolveContexts = Array( i ), + resolveValues = slice.call( arguments ), + + // the primary Deferred + primary = jQuery.Deferred(), + + // subordinate callback factory + updateFunc = function( i ) { + return function( value ) { + resolveContexts[ i ] = this; + resolveValues[ i ] = arguments.length > 1 ? slice.call( arguments ) : value; + if ( !( --remaining ) ) { + primary.resolveWith( resolveContexts, resolveValues ); + } + }; + }; + + // Single- and empty arguments are adopted like Promise.resolve + if ( remaining <= 1 ) { + adoptValue( singleValue, primary.done( updateFunc( i ) ).resolve, primary.reject, + !remaining ); + + // Use .then() to unwrap secondary thenables (cf. gh-3000) + if ( primary.state() === "pending" || + isFunction( resolveValues[ i ] && resolveValues[ i ].then ) ) { + + return primary.then(); + } + } + + // Multiple arguments are aggregated like Promise.all array elements + while ( i-- ) { + adoptValue( resolveValues[ i ], updateFunc( i ), primary.reject ); + } + + return primary.promise(); + } +} ); + + +// These usually indicate a programmer mistake during development, +// warn about them ASAP rather than swallowing them by default. +var rerrorNames = /^(Eval|Internal|Range|Reference|Syntax|Type|URI)Error$/; + +jQuery.Deferred.exceptionHook = function( error, stack ) { + + // Support: IE 8 - 9 only + // Console exists when dev tools are open, which can happen at any time + if ( window.console && window.console.warn && error && rerrorNames.test( error.name ) ) { + window.console.warn( "jQuery.Deferred exception: " + error.message, error.stack, stack ); + } +}; + + + + +jQuery.readyException = function( error ) { + window.setTimeout( function() { + throw error; + } ); +}; + + + + +// The deferred used on DOM ready +var readyList = jQuery.Deferred(); + +jQuery.fn.ready = function( fn ) { + + readyList + .then( fn ) + + // Wrap jQuery.readyException in a function so that the lookup + // happens at the time of error handling instead of callback + // registration. + .catch( function( error ) { + jQuery.readyException( error ); + } ); + + return this; +}; + +jQuery.extend( { + + // Is the DOM ready to be used? Set to true once it occurs. + isReady: false, + + // A counter to track how many items to wait for before + // the ready event fires. See #6781 + readyWait: 1, + + // Handle when the DOM is ready + ready: function( wait ) { + + // Abort if there are pending holds or we're already ready + if ( wait === true ? --jQuery.readyWait : jQuery.isReady ) { + return; + } + + // Remember that the DOM is ready + jQuery.isReady = true; + + // If a normal DOM Ready event fired, decrement, and wait if need be + if ( wait !== true && --jQuery.readyWait > 0 ) { + return; + } + + // If there are functions bound, to execute + readyList.resolveWith( document, [ jQuery ] ); + } +} ); + +jQuery.ready.then = readyList.then; + +// The ready event handler and self cleanup method +function completed() { + document.removeEventListener( "DOMContentLoaded", completed ); + window.removeEventListener( "load", completed ); + jQuery.ready(); +} + +// Catch cases where $(document).ready() is called +// after the browser event has already occurred. +// Support: IE <=9 - 10 only +// Older IE sometimes signals "interactive" too soon +if ( document.readyState === "complete" || + ( document.readyState !== "loading" && !document.documentElement.doScroll ) ) { + + // Handle it asynchronously to allow scripts the opportunity to delay ready + window.setTimeout( jQuery.ready ); + +} else { + + // Use the handy event callback + document.addEventListener( "DOMContentLoaded", completed ); + + // A fallback to window.onload, that will always work + window.addEventListener( "load", completed ); +} + + + + +// Multifunctional method to get and set values of a collection +// The value/s can optionally be executed if it's a function +var access = function( elems, fn, key, value, chainable, emptyGet, raw ) { + var i = 0, + len = elems.length, + bulk = key == null; + + // Sets many values + if ( toType( key ) === "object" ) { + chainable = true; + for ( i in key ) { + access( elems, fn, i, key[ i ], true, emptyGet, raw ); + } + + // Sets one value + } else if ( value !== undefined ) { + chainable = true; + + if ( !isFunction( value ) ) { + raw = true; + } + + if ( bulk ) { + + // Bulk operations run against the entire set + if ( raw ) { + fn.call( elems, value ); + fn = null; + + // ...except when executing function values + } else { + bulk = fn; + fn = function( elem, _key, value ) { + return bulk.call( jQuery( elem ), value ); + }; + } + } + + if ( fn ) { + for ( ; i < len; i++ ) { + fn( + elems[ i ], key, raw ? + value : + value.call( elems[ i ], i, fn( elems[ i ], key ) ) + ); + } + } + } + + if ( chainable ) { + return elems; + } + + // Gets + if ( bulk ) { + return fn.call( elems ); + } + + return len ? fn( elems[ 0 ], key ) : emptyGet; +}; + + +// Matches dashed string for camelizing +var rmsPrefix = /^-ms-/, + rdashAlpha = /-([a-z])/g; + +// Used by camelCase as callback to replace() +function fcamelCase( _all, letter ) { + return letter.toUpperCase(); +} + +// Convert dashed to camelCase; used by the css and data modules +// Support: IE <=9 - 11, Edge 12 - 15 +// Microsoft forgot to hump their vendor prefix (#9572) +function camelCase( string ) { + return string.replace( rmsPrefix, "ms-" ).replace( rdashAlpha, fcamelCase ); +} +var acceptData = function( owner ) { + + // Accepts only: + // - Node + // - Node.ELEMENT_NODE + // - Node.DOCUMENT_NODE + // - Object + // - Any + return owner.nodeType === 1 || owner.nodeType === 9 || !( +owner.nodeType ); +}; + + + + +function Data() { + this.expando = jQuery.expando + Data.uid++; +} + +Data.uid = 1; + +Data.prototype = { + + cache: function( owner ) { + + // Check if the owner object already has a cache + var value = owner[ this.expando ]; + + // If not, create one + if ( !value ) { + value = {}; + + // We can accept data for non-element nodes in modern browsers, + // but we should not, see #8335. + // Always return an empty object. + if ( acceptData( owner ) ) { + + // If it is a node unlikely to be stringify-ed or looped over + // use plain assignment + if ( owner.nodeType ) { + owner[ this.expando ] = value; + + // Otherwise secure it in a non-enumerable property + // configurable must be true to allow the property to be + // deleted when data is removed + } else { + Object.defineProperty( owner, this.expando, { + value: value, + configurable: true + } ); + } + } + } + + return value; + }, + set: function( owner, data, value ) { + var prop, + cache = this.cache( owner ); + + // Handle: [ owner, key, value ] args + // Always use camelCase key (gh-2257) + if ( typeof data === "string" ) { + cache[ camelCase( data ) ] = value; + + // Handle: [ owner, { properties } ] args + } else { + + // Copy the properties one-by-one to the cache object + for ( prop in data ) { + cache[ camelCase( prop ) ] = data[ prop ]; + } + } + return cache; + }, + get: function( owner, key ) { + return key === undefined ? + this.cache( owner ) : + + // Always use camelCase key (gh-2257) + owner[ this.expando ] && owner[ this.expando ][ camelCase( key ) ]; + }, + access: function( owner, key, value ) { + + // In cases where either: + // + // 1. No key was specified + // 2. A string key was specified, but no value provided + // + // Take the "read" path and allow the get method to determine + // which value to return, respectively either: + // + // 1. The entire cache object + // 2. The data stored at the key + // + if ( key === undefined || + ( ( key && typeof key === "string" ) && value === undefined ) ) { + + return this.get( owner, key ); + } + + // When the key is not a string, or both a key and value + // are specified, set or extend (existing objects) with either: + // + // 1. An object of properties + // 2. A key and value + // + this.set( owner, key, value ); + + // Since the "set" path can have two possible entry points + // return the expected data based on which path was taken[*] + return value !== undefined ? value : key; + }, + remove: function( owner, key ) { + var i, + cache = owner[ this.expando ]; + + if ( cache === undefined ) { + return; + } + + if ( key !== undefined ) { + + // Support array or space separated string of keys + if ( Array.isArray( key ) ) { + + // If key is an array of keys... + // We always set camelCase keys, so remove that. + key = key.map( camelCase ); + } else { + key = camelCase( key ); + + // If a key with the spaces exists, use it. + // Otherwise, create an array by matching non-whitespace + key = key in cache ? + [ key ] : + ( key.match( rnothtmlwhite ) || [] ); + } + + i = key.length; + + while ( i-- ) { + delete cache[ key[ i ] ]; + } + } + + // Remove the expando if there's no more data + if ( key === undefined || jQuery.isEmptyObject( cache ) ) { + + // Support: Chrome <=35 - 45 + // Webkit & Blink performance suffers when deleting properties + // from DOM nodes, so set to undefined instead + // https://bugs.chromium.org/p/chromium/issues/detail?id=378607 (bug restricted) + if ( owner.nodeType ) { + owner[ this.expando ] = undefined; + } else { + delete owner[ this.expando ]; + } + } + }, + hasData: function( owner ) { + var cache = owner[ this.expando ]; + return cache !== undefined && !jQuery.isEmptyObject( cache ); + } +}; +var dataPriv = new Data(); + +var dataUser = new Data(); + + + +// Implementation Summary +// +// 1. Enforce API surface and semantic compatibility with 1.9.x branch +// 2. Improve the module's maintainability by reducing the storage +// paths to a single mechanism. +// 3. Use the same single mechanism to support "private" and "user" data. +// 4. _Never_ expose "private" data to user code (TODO: Drop _data, _removeData) +// 5. Avoid exposing implementation details on user objects (eg. expando properties) +// 6. Provide a clear path for implementation upgrade to WeakMap in 2014 + +var rbrace = /^(?:\{[\w\W]*\}|\[[\w\W]*\])$/, + rmultiDash = /[A-Z]/g; + +function getData( data ) { + if ( data === "true" ) { + return true; + } + + if ( data === "false" ) { + return false; + } + + if ( data === "null" ) { + return null; + } + + // Only convert to a number if it doesn't change the string + if ( data === +data + "" ) { + return +data; + } + + if ( rbrace.test( data ) ) { + return JSON.parse( data ); + } + + return data; +} + +function dataAttr( elem, key, data ) { + var name; + + // If nothing was found internally, try to fetch any + // data from the HTML5 data-* attribute + if ( data === undefined && elem.nodeType === 1 ) { + name = "data-" + key.replace( rmultiDash, "-$&" ).toLowerCase(); + data = elem.getAttribute( name ); + + if ( typeof data === "string" ) { + try { + data = getData( data ); + } catch ( e ) {} + + // Make sure we set the data so it isn't changed later + dataUser.set( elem, key, data ); + } else { + data = undefined; + } + } + return data; +} + +jQuery.extend( { + hasData: function( elem ) { + return dataUser.hasData( elem ) || dataPriv.hasData( elem ); + }, + + data: function( elem, name, data ) { + return dataUser.access( elem, name, data ); + }, + + removeData: function( elem, name ) { + dataUser.remove( elem, name ); + }, + + // TODO: Now that all calls to _data and _removeData have been replaced + // with direct calls to dataPriv methods, these can be deprecated. + _data: function( elem, name, data ) { + return dataPriv.access( elem, name, data ); + }, + + _removeData: function( elem, name ) { + dataPriv.remove( elem, name ); + } +} ); + +jQuery.fn.extend( { + data: function( key, value ) { + var i, name, data, + elem = this[ 0 ], + attrs = elem && elem.attributes; + + // Gets all values + if ( key === undefined ) { + if ( this.length ) { + data = dataUser.get( elem ); + + if ( elem.nodeType === 1 && !dataPriv.get( elem, "hasDataAttrs" ) ) { + i = attrs.length; + while ( i-- ) { + + // Support: IE 11 only + // The attrs elements can be null (#14894) + if ( attrs[ i ] ) { + name = attrs[ i ].name; + if ( name.indexOf( "data-" ) === 0 ) { + name = camelCase( name.slice( 5 ) ); + dataAttr( elem, name, data[ name ] ); + } + } + } + dataPriv.set( elem, "hasDataAttrs", true ); + } + } + + return data; + } + + // Sets multiple values + if ( typeof key === "object" ) { + return this.each( function() { + dataUser.set( this, key ); + } ); + } + + return access( this, function( value ) { + var data; + + // The calling jQuery object (element matches) is not empty + // (and therefore has an element appears at this[ 0 ]) and the + // `value` parameter was not undefined. An empty jQuery object + // will result in `undefined` for elem = this[ 0 ] which will + // throw an exception if an attempt to read a data cache is made. + if ( elem && value === undefined ) { + + // Attempt to get data from the cache + // The key will always be camelCased in Data + data = dataUser.get( elem, key ); + if ( data !== undefined ) { + return data; + } + + // Attempt to "discover" the data in + // HTML5 custom data-* attrs + data = dataAttr( elem, key ); + if ( data !== undefined ) { + return data; + } + + // We tried really hard, but the data doesn't exist. + return; + } + + // Set the data... + this.each( function() { + + // We always store the camelCased key + dataUser.set( this, key, value ); + } ); + }, null, value, arguments.length > 1, null, true ); + }, + + removeData: function( key ) { + return this.each( function() { + dataUser.remove( this, key ); + } ); + } +} ); + + +jQuery.extend( { + queue: function( elem, type, data ) { + var queue; + + if ( elem ) { + type = ( type || "fx" ) + "queue"; + queue = dataPriv.get( elem, type ); + + // Speed up dequeue by getting out quickly if this is just a lookup + if ( data ) { + if ( !queue || Array.isArray( data ) ) { + queue = dataPriv.access( elem, type, jQuery.makeArray( data ) ); + } else { + queue.push( data ); + } + } + return queue || []; + } + }, + + dequeue: function( elem, type ) { + type = type || "fx"; + + var queue = jQuery.queue( elem, type ), + startLength = queue.length, + fn = queue.shift(), + hooks = jQuery._queueHooks( elem, type ), + next = function() { + jQuery.dequeue( elem, type ); + }; + + // If the fx queue is dequeued, always remove the progress sentinel + if ( fn === "inprogress" ) { + fn = queue.shift(); + startLength--; + } + + if ( fn ) { + + // Add a progress sentinel to prevent the fx queue from being + // automatically dequeued + if ( type === "fx" ) { + queue.unshift( "inprogress" ); + } + + // Clear up the last queue stop function + delete hooks.stop; + fn.call( elem, next, hooks ); + } + + if ( !startLength && hooks ) { + hooks.empty.fire(); + } + }, + + // Not public - generate a queueHooks object, or return the current one + _queueHooks: function( elem, type ) { + var key = type + "queueHooks"; + return dataPriv.get( elem, key ) || dataPriv.access( elem, key, { + empty: jQuery.Callbacks( "once memory" ).add( function() { + dataPriv.remove( elem, [ type + "queue", key ] ); + } ) + } ); + } +} ); + +jQuery.fn.extend( { + queue: function( type, data ) { + var setter = 2; + + if ( typeof type !== "string" ) { + data = type; + type = "fx"; + setter--; + } + + if ( arguments.length < setter ) { + return jQuery.queue( this[ 0 ], type ); + } + + return data === undefined ? + this : + this.each( function() { + var queue = jQuery.queue( this, type, data ); + + // Ensure a hooks for this queue + jQuery._queueHooks( this, type ); + + if ( type === "fx" && queue[ 0 ] !== "inprogress" ) { + jQuery.dequeue( this, type ); + } + } ); + }, + dequeue: function( type ) { + return this.each( function() { + jQuery.dequeue( this, type ); + } ); + }, + clearQueue: function( type ) { + return this.queue( type || "fx", [] ); + }, + + // Get a promise resolved when queues of a certain type + // are emptied (fx is the type by default) + promise: function( type, obj ) { + var tmp, + count = 1, + defer = jQuery.Deferred(), + elements = this, + i = this.length, + resolve = function() { + if ( !( --count ) ) { + defer.resolveWith( elements, [ elements ] ); + } + }; + + if ( typeof type !== "string" ) { + obj = type; + type = undefined; + } + type = type || "fx"; + + while ( i-- ) { + tmp = dataPriv.get( elements[ i ], type + "queueHooks" ); + if ( tmp && tmp.empty ) { + count++; + tmp.empty.add( resolve ); + } + } + resolve(); + return defer.promise( obj ); + } +} ); +var pnum = ( /[+-]?(?:\d*\.|)\d+(?:[eE][+-]?\d+|)/ ).source; + +var rcssNum = new RegExp( "^(?:([+-])=|)(" + pnum + ")([a-z%]*)$", "i" ); + + +var cssExpand = [ "Top", "Right", "Bottom", "Left" ]; + +var documentElement = document.documentElement; + + + + var isAttached = function( elem ) { + return jQuery.contains( elem.ownerDocument, elem ); + }, + composed = { composed: true }; + + // Support: IE 9 - 11+, Edge 12 - 18+, iOS 10.0 - 10.2 only + // Check attachment across shadow DOM boundaries when possible (gh-3504) + // Support: iOS 10.0-10.2 only + // Early iOS 10 versions support `attachShadow` but not `getRootNode`, + // leading to errors. We need to check for `getRootNode`. + if ( documentElement.getRootNode ) { + isAttached = function( elem ) { + return jQuery.contains( elem.ownerDocument, elem ) || + elem.getRootNode( composed ) === elem.ownerDocument; + }; + } +var isHiddenWithinTree = function( elem, el ) { + + // isHiddenWithinTree might be called from jQuery#filter function; + // in that case, element will be second argument + elem = el || elem; + + // Inline style trumps all + return elem.style.display === "none" || + elem.style.display === "" && + + // Otherwise, check computed style + // Support: Firefox <=43 - 45 + // Disconnected elements can have computed display: none, so first confirm that elem is + // in the document. + isAttached( elem ) && + + jQuery.css( elem, "display" ) === "none"; + }; + + + +function adjustCSS( elem, prop, valueParts, tween ) { + var adjusted, scale, + maxIterations = 20, + currentValue = tween ? + function() { + return tween.cur(); + } : + function() { + return jQuery.css( elem, prop, "" ); + }, + initial = currentValue(), + unit = valueParts && valueParts[ 3 ] || ( jQuery.cssNumber[ prop ] ? "" : "px" ), + + // Starting value computation is required for potential unit mismatches + initialInUnit = elem.nodeType && + ( jQuery.cssNumber[ prop ] || unit !== "px" && +initial ) && + rcssNum.exec( jQuery.css( elem, prop ) ); + + if ( initialInUnit && initialInUnit[ 3 ] !== unit ) { + + // Support: Firefox <=54 + // Halve the iteration target value to prevent interference from CSS upper bounds (gh-2144) + initial = initial / 2; + + // Trust units reported by jQuery.css + unit = unit || initialInUnit[ 3 ]; + + // Iteratively approximate from a nonzero starting point + initialInUnit = +initial || 1; + + while ( maxIterations-- ) { + + // Evaluate and update our best guess (doubling guesses that zero out). + // Finish if the scale equals or crosses 1 (making the old*new product non-positive). + jQuery.style( elem, prop, initialInUnit + unit ); + if ( ( 1 - scale ) * ( 1 - ( scale = currentValue() / initial || 0.5 ) ) <= 0 ) { + maxIterations = 0; + } + initialInUnit = initialInUnit / scale; + + } + + initialInUnit = initialInUnit * 2; + jQuery.style( elem, prop, initialInUnit + unit ); + + // Make sure we update the tween properties later on + valueParts = valueParts || []; + } + + if ( valueParts ) { + initialInUnit = +initialInUnit || +initial || 0; + + // Apply relative offset (+=/-=) if specified + adjusted = valueParts[ 1 ] ? + initialInUnit + ( valueParts[ 1 ] + 1 ) * valueParts[ 2 ] : + +valueParts[ 2 ]; + if ( tween ) { + tween.unit = unit; + tween.start = initialInUnit; + tween.end = adjusted; + } + } + return adjusted; +} + + +var defaultDisplayMap = {}; + +function getDefaultDisplay( elem ) { + var temp, + doc = elem.ownerDocument, + nodeName = elem.nodeName, + display = defaultDisplayMap[ nodeName ]; + + if ( display ) { + return display; + } + + temp = doc.body.appendChild( doc.createElement( nodeName ) ); + display = jQuery.css( temp, "display" ); + + temp.parentNode.removeChild( temp ); + + if ( display === "none" ) { + display = "block"; + } + defaultDisplayMap[ nodeName ] = display; + + return display; +} + +function showHide( elements, show ) { + var display, elem, + values = [], + index = 0, + length = elements.length; + + // Determine new display value for elements that need to change + for ( ; index < length; index++ ) { + elem = elements[ index ]; + if ( !elem.style ) { + continue; + } + + display = elem.style.display; + if ( show ) { + + // Since we force visibility upon cascade-hidden elements, an immediate (and slow) + // check is required in this first loop unless we have a nonempty display value (either + // inline or about-to-be-restored) + if ( display === "none" ) { + values[ index ] = dataPriv.get( elem, "display" ) || null; + if ( !values[ index ] ) { + elem.style.display = ""; + } + } + if ( elem.style.display === "" && isHiddenWithinTree( elem ) ) { + values[ index ] = getDefaultDisplay( elem ); + } + } else { + if ( display !== "none" ) { + values[ index ] = "none"; + + // Remember what we're overwriting + dataPriv.set( elem, "display", display ); + } + } + } + + // Set the display of the elements in a second loop to avoid constant reflow + for ( index = 0; index < length; index++ ) { + if ( values[ index ] != null ) { + elements[ index ].style.display = values[ index ]; + } + } + + return elements; +} + +jQuery.fn.extend( { + show: function() { + return showHide( this, true ); + }, + hide: function() { + return showHide( this ); + }, + toggle: function( state ) { + if ( typeof state === "boolean" ) { + return state ? this.show() : this.hide(); + } + + return this.each( function() { + if ( isHiddenWithinTree( this ) ) { + jQuery( this ).show(); + } else { + jQuery( this ).hide(); + } + } ); + } +} ); +var rcheckableType = ( /^(?:checkbox|radio)$/i ); + +var rtagName = ( /<([a-z][^\/\0>\x20\t\r\n\f]*)/i ); + +var rscriptType = ( /^$|^module$|\/(?:java|ecma)script/i ); + + + +( function() { + var fragment = document.createDocumentFragment(), + div = fragment.appendChild( document.createElement( "div" ) ), + input = document.createElement( "input" ); + + // Support: Android 4.0 - 4.3 only + // Check state lost if the name is set (#11217) + // Support: Windows Web Apps (WWA) + // `name` and `type` must use .setAttribute for WWA (#14901) + input.setAttribute( "type", "radio" ); + input.setAttribute( "checked", "checked" ); + input.setAttribute( "name", "t" ); + + div.appendChild( input ); + + // Support: Android <=4.1 only + // Older WebKit doesn't clone checked state correctly in fragments + support.checkClone = div.cloneNode( true ).cloneNode( true ).lastChild.checked; + + // Support: IE <=11 only + // Make sure textarea (and checkbox) defaultValue is properly cloned + div.innerHTML = ""; + support.noCloneChecked = !!div.cloneNode( true ).lastChild.defaultValue; + + // Support: IE <=9 only + // IE <=9 replaces "; + support.option = !!div.lastChild; +} )(); + + +// We have to close these tags to support XHTML (#13200) +var wrapMap = { + + // XHTML parsers do not magically insert elements in the + // same way that tag soup parsers do. So we cannot shorten + // this by omitting or other required elements. + thead: [ 1, "", "
" ], + col: [ 2, "", "
" ], + tr: [ 2, "", "
" ], + td: [ 3, "", "
" ], + + _default: [ 0, "", "" ] +}; + +wrapMap.tbody = wrapMap.tfoot = wrapMap.colgroup = wrapMap.caption = wrapMap.thead; +wrapMap.th = wrapMap.td; + +// Support: IE <=9 only +if ( !support.option ) { + wrapMap.optgroup = wrapMap.option = [ 1, "" ]; +} + + +function getAll( context, tag ) { + + // Support: IE <=9 - 11 only + // Use typeof to avoid zero-argument method invocation on host objects (#15151) + var ret; + + if ( typeof context.getElementsByTagName !== "undefined" ) { + ret = context.getElementsByTagName( tag || "*" ); + + } else if ( typeof context.querySelectorAll !== "undefined" ) { + ret = context.querySelectorAll( tag || "*" ); + + } else { + ret = []; + } + + if ( tag === undefined || tag && nodeName( context, tag ) ) { + return jQuery.merge( [ context ], ret ); + } + + return ret; +} + + +// Mark scripts as having already been evaluated +function setGlobalEval( elems, refElements ) { + var i = 0, + l = elems.length; + + for ( ; i < l; i++ ) { + dataPriv.set( + elems[ i ], + "globalEval", + !refElements || dataPriv.get( refElements[ i ], "globalEval" ) + ); + } +} + + +var rhtml = /<|&#?\w+;/; + +function buildFragment( elems, context, scripts, selection, ignored ) { + var elem, tmp, tag, wrap, attached, j, + fragment = context.createDocumentFragment(), + nodes = [], + i = 0, + l = elems.length; + + for ( ; i < l; i++ ) { + elem = elems[ i ]; + + if ( elem || elem === 0 ) { + + // Add nodes directly + if ( toType( elem ) === "object" ) { + + // Support: Android <=4.0 only, PhantomJS 1 only + // push.apply(_, arraylike) throws on ancient WebKit + jQuery.merge( nodes, elem.nodeType ? [ elem ] : elem ); + + // Convert non-html into a text node + } else if ( !rhtml.test( elem ) ) { + nodes.push( context.createTextNode( elem ) ); + + // Convert html into DOM nodes + } else { + tmp = tmp || fragment.appendChild( context.createElement( "div" ) ); + + // Deserialize a standard representation + tag = ( rtagName.exec( elem ) || [ "", "" ] )[ 1 ].toLowerCase(); + wrap = wrapMap[ tag ] || wrapMap._default; + tmp.innerHTML = wrap[ 1 ] + jQuery.htmlPrefilter( elem ) + wrap[ 2 ]; + + // Descend through wrappers to the right content + j = wrap[ 0 ]; + while ( j-- ) { + tmp = tmp.lastChild; + } + + // Support: Android <=4.0 only, PhantomJS 1 only + // push.apply(_, arraylike) throws on ancient WebKit + jQuery.merge( nodes, tmp.childNodes ); + + // Remember the top-level container + tmp = fragment.firstChild; + + // Ensure the created nodes are orphaned (#12392) + tmp.textContent = ""; + } + } + } + + // Remove wrapper from fragment + fragment.textContent = ""; + + i = 0; + while ( ( elem = nodes[ i++ ] ) ) { + + // Skip elements already in the context collection (trac-4087) + if ( selection && jQuery.inArray( elem, selection ) > -1 ) { + if ( ignored ) { + ignored.push( elem ); + } + continue; + } + + attached = isAttached( elem ); + + // Append to fragment + tmp = getAll( fragment.appendChild( elem ), "script" ); + + // Preserve script evaluation history + if ( attached ) { + setGlobalEval( tmp ); + } + + // Capture executables + if ( scripts ) { + j = 0; + while ( ( elem = tmp[ j++ ] ) ) { + if ( rscriptType.test( elem.type || "" ) ) { + scripts.push( elem ); + } + } + } + } + + return fragment; +} + + +var rtypenamespace = /^([^.]*)(?:\.(.+)|)/; + +function returnTrue() { + return true; +} + +function returnFalse() { + return false; +} + +// Support: IE <=9 - 11+ +// focus() and blur() are asynchronous, except when they are no-op. +// So expect focus to be synchronous when the element is already active, +// and blur to be synchronous when the element is not already active. +// (focus and blur are always synchronous in other supported browsers, +// this just defines when we can count on it). +function expectSync( elem, type ) { + return ( elem === safeActiveElement() ) === ( type === "focus" ); +} + +// Support: IE <=9 only +// Accessing document.activeElement can throw unexpectedly +// https://bugs.jquery.com/ticket/13393 +function safeActiveElement() { + try { + return document.activeElement; + } catch ( err ) { } +} + +function on( elem, types, selector, data, fn, one ) { + var origFn, type; + + // Types can be a map of types/handlers + if ( typeof types === "object" ) { + + // ( types-Object, selector, data ) + if ( typeof selector !== "string" ) { + + // ( types-Object, data ) + data = data || selector; + selector = undefined; + } + for ( type in types ) { + on( elem, type, selector, data, types[ type ], one ); + } + return elem; + } + + if ( data == null && fn == null ) { + + // ( types, fn ) + fn = selector; + data = selector = undefined; + } else if ( fn == null ) { + if ( typeof selector === "string" ) { + + // ( types, selector, fn ) + fn = data; + data = undefined; + } else { + + // ( types, data, fn ) + fn = data; + data = selector; + selector = undefined; + } + } + if ( fn === false ) { + fn = returnFalse; + } else if ( !fn ) { + return elem; + } + + if ( one === 1 ) { + origFn = fn; + fn = function( event ) { + + // Can use an empty set, since event contains the info + jQuery().off( event ); + return origFn.apply( this, arguments ); + }; + + // Use same guid so caller can remove using origFn + fn.guid = origFn.guid || ( origFn.guid = jQuery.guid++ ); + } + return elem.each( function() { + jQuery.event.add( this, types, fn, data, selector ); + } ); +} + +/* + * Helper functions for managing events -- not part of the public interface. + * Props to Dean Edwards' addEvent library for many of the ideas. + */ +jQuery.event = { + + global: {}, + + add: function( elem, types, handler, data, selector ) { + + var handleObjIn, eventHandle, tmp, + events, t, handleObj, + special, handlers, type, namespaces, origType, + elemData = dataPriv.get( elem ); + + // Only attach events to objects that accept data + if ( !acceptData( elem ) ) { + return; + } + + // Caller can pass in an object of custom data in lieu of the handler + if ( handler.handler ) { + handleObjIn = handler; + handler = handleObjIn.handler; + selector = handleObjIn.selector; + } + + // Ensure that invalid selectors throw exceptions at attach time + // Evaluate against documentElement in case elem is a non-element node (e.g., document) + if ( selector ) { + jQuery.find.matchesSelector( documentElement, selector ); + } + + // Make sure that the handler has a unique ID, used to find/remove it later + if ( !handler.guid ) { + handler.guid = jQuery.guid++; + } + + // Init the element's event structure and main handler, if this is the first + if ( !( events = elemData.events ) ) { + events = elemData.events = Object.create( null ); + } + if ( !( eventHandle = elemData.handle ) ) { + eventHandle = elemData.handle = function( e ) { + + // Discard the second event of a jQuery.event.trigger() and + // when an event is called after a page has unloaded + return typeof jQuery !== "undefined" && jQuery.event.triggered !== e.type ? + jQuery.event.dispatch.apply( elem, arguments ) : undefined; + }; + } + + // Handle multiple events separated by a space + types = ( types || "" ).match( rnothtmlwhite ) || [ "" ]; + t = types.length; + while ( t-- ) { + tmp = rtypenamespace.exec( types[ t ] ) || []; + type = origType = tmp[ 1 ]; + namespaces = ( tmp[ 2 ] || "" ).split( "." ).sort(); + + // There *must* be a type, no attaching namespace-only handlers + if ( !type ) { + continue; + } + + // If event changes its type, use the special event handlers for the changed type + special = jQuery.event.special[ type ] || {}; + + // If selector defined, determine special event api type, otherwise given type + type = ( selector ? special.delegateType : special.bindType ) || type; + + // Update special based on newly reset type + special = jQuery.event.special[ type ] || {}; + + // handleObj is passed to all event handlers + handleObj = jQuery.extend( { + type: type, + origType: origType, + data: data, + handler: handler, + guid: handler.guid, + selector: selector, + needsContext: selector && jQuery.expr.match.needsContext.test( selector ), + namespace: namespaces.join( "." ) + }, handleObjIn ); + + // Init the event handler queue if we're the first + if ( !( handlers = events[ type ] ) ) { + handlers = events[ type ] = []; + handlers.delegateCount = 0; + + // Only use addEventListener if the special events handler returns false + if ( !special.setup || + special.setup.call( elem, data, namespaces, eventHandle ) === false ) { + + if ( elem.addEventListener ) { + elem.addEventListener( type, eventHandle ); + } + } + } + + if ( special.add ) { + special.add.call( elem, handleObj ); + + if ( !handleObj.handler.guid ) { + handleObj.handler.guid = handler.guid; + } + } + + // Add to the element's handler list, delegates in front + if ( selector ) { + handlers.splice( handlers.delegateCount++, 0, handleObj ); + } else { + handlers.push( handleObj ); + } + + // Keep track of which events have ever been used, for event optimization + jQuery.event.global[ type ] = true; + } + + }, + + // Detach an event or set of events from an element + remove: function( elem, types, handler, selector, mappedTypes ) { + + var j, origCount, tmp, + events, t, handleObj, + special, handlers, type, namespaces, origType, + elemData = dataPriv.hasData( elem ) && dataPriv.get( elem ); + + if ( !elemData || !( events = elemData.events ) ) { + return; + } + + // Once for each type.namespace in types; type may be omitted + types = ( types || "" ).match( rnothtmlwhite ) || [ "" ]; + t = types.length; + while ( t-- ) { + tmp = rtypenamespace.exec( types[ t ] ) || []; + type = origType = tmp[ 1 ]; + namespaces = ( tmp[ 2 ] || "" ).split( "." ).sort(); + + // Unbind all events (on this namespace, if provided) for the element + if ( !type ) { + for ( type in events ) { + jQuery.event.remove( elem, type + types[ t ], handler, selector, true ); + } + continue; + } + + special = jQuery.event.special[ type ] || {}; + type = ( selector ? special.delegateType : special.bindType ) || type; + handlers = events[ type ] || []; + tmp = tmp[ 2 ] && + new RegExp( "(^|\\.)" + namespaces.join( "\\.(?:.*\\.|)" ) + "(\\.|$)" ); + + // Remove matching events + origCount = j = handlers.length; + while ( j-- ) { + handleObj = handlers[ j ]; + + if ( ( mappedTypes || origType === handleObj.origType ) && + ( !handler || handler.guid === handleObj.guid ) && + ( !tmp || tmp.test( handleObj.namespace ) ) && + ( !selector || selector === handleObj.selector || + selector === "**" && handleObj.selector ) ) { + handlers.splice( j, 1 ); + + if ( handleObj.selector ) { + handlers.delegateCount--; + } + if ( special.remove ) { + special.remove.call( elem, handleObj ); + } + } + } + + // Remove generic event handler if we removed something and no more handlers exist + // (avoids potential for endless recursion during removal of special event handlers) + if ( origCount && !handlers.length ) { + if ( !special.teardown || + special.teardown.call( elem, namespaces, elemData.handle ) === false ) { + + jQuery.removeEvent( elem, type, elemData.handle ); + } + + delete events[ type ]; + } + } + + // Remove data and the expando if it's no longer used + if ( jQuery.isEmptyObject( events ) ) { + dataPriv.remove( elem, "handle events" ); + } + }, + + dispatch: function( nativeEvent ) { + + var i, j, ret, matched, handleObj, handlerQueue, + args = new Array( arguments.length ), + + // Make a writable jQuery.Event from the native event object + event = jQuery.event.fix( nativeEvent ), + + handlers = ( + dataPriv.get( this, "events" ) || Object.create( null ) + )[ event.type ] || [], + special = jQuery.event.special[ event.type ] || {}; + + // Use the fix-ed jQuery.Event rather than the (read-only) native event + args[ 0 ] = event; + + for ( i = 1; i < arguments.length; i++ ) { + args[ i ] = arguments[ i ]; + } + + event.delegateTarget = this; + + // Call the preDispatch hook for the mapped type, and let it bail if desired + if ( special.preDispatch && special.preDispatch.call( this, event ) === false ) { + return; + } + + // Determine handlers + handlerQueue = jQuery.event.handlers.call( this, event, handlers ); + + // Run delegates first; they may want to stop propagation beneath us + i = 0; + while ( ( matched = handlerQueue[ i++ ] ) && !event.isPropagationStopped() ) { + event.currentTarget = matched.elem; + + j = 0; + while ( ( handleObj = matched.handlers[ j++ ] ) && + !event.isImmediatePropagationStopped() ) { + + // If the event is namespaced, then each handler is only invoked if it is + // specially universal or its namespaces are a superset of the event's. + if ( !event.rnamespace || handleObj.namespace === false || + event.rnamespace.test( handleObj.namespace ) ) { + + event.handleObj = handleObj; + event.data = handleObj.data; + + ret = ( ( jQuery.event.special[ handleObj.origType ] || {} ).handle || + handleObj.handler ).apply( matched.elem, args ); + + if ( ret !== undefined ) { + if ( ( event.result = ret ) === false ) { + event.preventDefault(); + event.stopPropagation(); + } + } + } + } + } + + // Call the postDispatch hook for the mapped type + if ( special.postDispatch ) { + special.postDispatch.call( this, event ); + } + + return event.result; + }, + + handlers: function( event, handlers ) { + var i, handleObj, sel, matchedHandlers, matchedSelectors, + handlerQueue = [], + delegateCount = handlers.delegateCount, + cur = event.target; + + // Find delegate handlers + if ( delegateCount && + + // Support: IE <=9 + // Black-hole SVG instance trees (trac-13180) + cur.nodeType && + + // Support: Firefox <=42 + // Suppress spec-violating clicks indicating a non-primary pointer button (trac-3861) + // https://www.w3.org/TR/DOM-Level-3-Events/#event-type-click + // Support: IE 11 only + // ...but not arrow key "clicks" of radio inputs, which can have `button` -1 (gh-2343) + !( event.type === "click" && event.button >= 1 ) ) { + + for ( ; cur !== this; cur = cur.parentNode || this ) { + + // Don't check non-elements (#13208) + // Don't process clicks on disabled elements (#6911, #8165, #11382, #11764) + if ( cur.nodeType === 1 && !( event.type === "click" && cur.disabled === true ) ) { + matchedHandlers = []; + matchedSelectors = {}; + for ( i = 0; i < delegateCount; i++ ) { + handleObj = handlers[ i ]; + + // Don't conflict with Object.prototype properties (#13203) + sel = handleObj.selector + " "; + + if ( matchedSelectors[ sel ] === undefined ) { + matchedSelectors[ sel ] = handleObj.needsContext ? + jQuery( sel, this ).index( cur ) > -1 : + jQuery.find( sel, this, null, [ cur ] ).length; + } + if ( matchedSelectors[ sel ] ) { + matchedHandlers.push( handleObj ); + } + } + if ( matchedHandlers.length ) { + handlerQueue.push( { elem: cur, handlers: matchedHandlers } ); + } + } + } + } + + // Add the remaining (directly-bound) handlers + cur = this; + if ( delegateCount < handlers.length ) { + handlerQueue.push( { elem: cur, handlers: handlers.slice( delegateCount ) } ); + } + + return handlerQueue; + }, + + addProp: function( name, hook ) { + Object.defineProperty( jQuery.Event.prototype, name, { + enumerable: true, + configurable: true, + + get: isFunction( hook ) ? + function() { + if ( this.originalEvent ) { + return hook( this.originalEvent ); + } + } : + function() { + if ( this.originalEvent ) { + return this.originalEvent[ name ]; + } + }, + + set: function( value ) { + Object.defineProperty( this, name, { + enumerable: true, + configurable: true, + writable: true, + value: value + } ); + } + } ); + }, + + fix: function( originalEvent ) { + return originalEvent[ jQuery.expando ] ? + originalEvent : + new jQuery.Event( originalEvent ); + }, + + special: { + load: { + + // Prevent triggered image.load events from bubbling to window.load + noBubble: true + }, + click: { + + // Utilize native event to ensure correct state for checkable inputs + setup: function( data ) { + + // For mutual compressibility with _default, replace `this` access with a local var. + // `|| data` is dead code meant only to preserve the variable through minification. + var el = this || data; + + // Claim the first handler + if ( rcheckableType.test( el.type ) && + el.click && nodeName( el, "input" ) ) { + + // dataPriv.set( el, "click", ... ) + leverageNative( el, "click", returnTrue ); + } + + // Return false to allow normal processing in the caller + return false; + }, + trigger: function( data ) { + + // For mutual compressibility with _default, replace `this` access with a local var. + // `|| data` is dead code meant only to preserve the variable through minification. + var el = this || data; + + // Force setup before triggering a click + if ( rcheckableType.test( el.type ) && + el.click && nodeName( el, "input" ) ) { + + leverageNative( el, "click" ); + } + + // Return non-false to allow normal event-path propagation + return true; + }, + + // For cross-browser consistency, suppress native .click() on links + // Also prevent it if we're currently inside a leveraged native-event stack + _default: function( event ) { + var target = event.target; + return rcheckableType.test( target.type ) && + target.click && nodeName( target, "input" ) && + dataPriv.get( target, "click" ) || + nodeName( target, "a" ); + } + }, + + beforeunload: { + postDispatch: function( event ) { + + // Support: Firefox 20+ + // Firefox doesn't alert if the returnValue field is not set. + if ( event.result !== undefined && event.originalEvent ) { + event.originalEvent.returnValue = event.result; + } + } + } + } +}; + +// Ensure the presence of an event listener that handles manually-triggered +// synthetic events by interrupting progress until reinvoked in response to +// *native* events that it fires directly, ensuring that state changes have +// already occurred before other listeners are invoked. +function leverageNative( el, type, expectSync ) { + + // Missing expectSync indicates a trigger call, which must force setup through jQuery.event.add + if ( !expectSync ) { + if ( dataPriv.get( el, type ) === undefined ) { + jQuery.event.add( el, type, returnTrue ); + } + return; + } + + // Register the controller as a special universal handler for all event namespaces + dataPriv.set( el, type, false ); + jQuery.event.add( el, type, { + namespace: false, + handler: function( event ) { + var notAsync, result, + saved = dataPriv.get( this, type ); + + if ( ( event.isTrigger & 1 ) && this[ type ] ) { + + // Interrupt processing of the outer synthetic .trigger()ed event + // Saved data should be false in such cases, but might be a leftover capture object + // from an async native handler (gh-4350) + if ( !saved.length ) { + + // Store arguments for use when handling the inner native event + // There will always be at least one argument (an event object), so this array + // will not be confused with a leftover capture object. + saved = slice.call( arguments ); + dataPriv.set( this, type, saved ); + + // Trigger the native event and capture its result + // Support: IE <=9 - 11+ + // focus() and blur() are asynchronous + notAsync = expectSync( this, type ); + this[ type ](); + result = dataPriv.get( this, type ); + if ( saved !== result || notAsync ) { + dataPriv.set( this, type, false ); + } else { + result = {}; + } + if ( saved !== result ) { + + // Cancel the outer synthetic event + event.stopImmediatePropagation(); + event.preventDefault(); + + // Support: Chrome 86+ + // In Chrome, if an element having a focusout handler is blurred by + // clicking outside of it, it invokes the handler synchronously. If + // that handler calls `.remove()` on the element, the data is cleared, + // leaving `result` undefined. We need to guard against this. + return result && result.value; + } + + // If this is an inner synthetic event for an event with a bubbling surrogate + // (focus or blur), assume that the surrogate already propagated from triggering the + // native event and prevent that from happening again here. + // This technically gets the ordering wrong w.r.t. to `.trigger()` (in which the + // bubbling surrogate propagates *after* the non-bubbling base), but that seems + // less bad than duplication. + } else if ( ( jQuery.event.special[ type ] || {} ).delegateType ) { + event.stopPropagation(); + } + + // If this is a native event triggered above, everything is now in order + // Fire an inner synthetic event with the original arguments + } else if ( saved.length ) { + + // ...and capture the result + dataPriv.set( this, type, { + value: jQuery.event.trigger( + + // Support: IE <=9 - 11+ + // Extend with the prototype to reset the above stopImmediatePropagation() + jQuery.extend( saved[ 0 ], jQuery.Event.prototype ), + saved.slice( 1 ), + this + ) + } ); + + // Abort handling of the native event + event.stopImmediatePropagation(); + } + } + } ); +} + +jQuery.removeEvent = function( elem, type, handle ) { + + // This "if" is needed for plain objects + if ( elem.removeEventListener ) { + elem.removeEventListener( type, handle ); + } +}; + +jQuery.Event = function( src, props ) { + + // Allow instantiation without the 'new' keyword + if ( !( this instanceof jQuery.Event ) ) { + return new jQuery.Event( src, props ); + } + + // Event object + if ( src && src.type ) { + this.originalEvent = src; + this.type = src.type; + + // Events bubbling up the document may have been marked as prevented + // by a handler lower down the tree; reflect the correct value. + this.isDefaultPrevented = src.defaultPrevented || + src.defaultPrevented === undefined && + + // Support: Android <=2.3 only + src.returnValue === false ? + returnTrue : + returnFalse; + + // Create target properties + // Support: Safari <=6 - 7 only + // Target should not be a text node (#504, #13143) + this.target = ( src.target && src.target.nodeType === 3 ) ? + src.target.parentNode : + src.target; + + this.currentTarget = src.currentTarget; + this.relatedTarget = src.relatedTarget; + + // Event type + } else { + this.type = src; + } + + // Put explicitly provided properties onto the event object + if ( props ) { + jQuery.extend( this, props ); + } + + // Create a timestamp if incoming event doesn't have one + this.timeStamp = src && src.timeStamp || Date.now(); + + // Mark it as fixed + this[ jQuery.expando ] = true; +}; + +// jQuery.Event is based on DOM3 Events as specified by the ECMAScript Language Binding +// https://www.w3.org/TR/2003/WD-DOM-Level-3-Events-20030331/ecma-script-binding.html +jQuery.Event.prototype = { + constructor: jQuery.Event, + isDefaultPrevented: returnFalse, + isPropagationStopped: returnFalse, + isImmediatePropagationStopped: returnFalse, + isSimulated: false, + + preventDefault: function() { + var e = this.originalEvent; + + this.isDefaultPrevented = returnTrue; + + if ( e && !this.isSimulated ) { + e.preventDefault(); + } + }, + stopPropagation: function() { + var e = this.originalEvent; + + this.isPropagationStopped = returnTrue; + + if ( e && !this.isSimulated ) { + e.stopPropagation(); + } + }, + stopImmediatePropagation: function() { + var e = this.originalEvent; + + this.isImmediatePropagationStopped = returnTrue; + + if ( e && !this.isSimulated ) { + e.stopImmediatePropagation(); + } + + this.stopPropagation(); + } +}; + +// Includes all common event props including KeyEvent and MouseEvent specific props +jQuery.each( { + altKey: true, + bubbles: true, + cancelable: true, + changedTouches: true, + ctrlKey: true, + detail: true, + eventPhase: true, + metaKey: true, + pageX: true, + pageY: true, + shiftKey: true, + view: true, + "char": true, + code: true, + charCode: true, + key: true, + keyCode: true, + button: true, + buttons: true, + clientX: true, + clientY: true, + offsetX: true, + offsetY: true, + pointerId: true, + pointerType: true, + screenX: true, + screenY: true, + targetTouches: true, + toElement: true, + touches: true, + which: true +}, jQuery.event.addProp ); + +jQuery.each( { focus: "focusin", blur: "focusout" }, function( type, delegateType ) { + jQuery.event.special[ type ] = { + + // Utilize native event if possible so blur/focus sequence is correct + setup: function() { + + // Claim the first handler + // dataPriv.set( this, "focus", ... ) + // dataPriv.set( this, "blur", ... ) + leverageNative( this, type, expectSync ); + + // Return false to allow normal processing in the caller + return false; + }, + trigger: function() { + + // Force setup before trigger + leverageNative( this, type ); + + // Return non-false to allow normal event-path propagation + return true; + }, + + // Suppress native focus or blur as it's already being fired + // in leverageNative. + _default: function() { + return true; + }, + + delegateType: delegateType + }; +} ); + +// Create mouseenter/leave events using mouseover/out and event-time checks +// so that event delegation works in jQuery. +// Do the same for pointerenter/pointerleave and pointerover/pointerout +// +// Support: Safari 7 only +// Safari sends mouseenter too often; see: +// https://bugs.chromium.org/p/chromium/issues/detail?id=470258 +// for the description of the bug (it existed in older Chrome versions as well). +jQuery.each( { + mouseenter: "mouseover", + mouseleave: "mouseout", + pointerenter: "pointerover", + pointerleave: "pointerout" +}, function( orig, fix ) { + jQuery.event.special[ orig ] = { + delegateType: fix, + bindType: fix, + + handle: function( event ) { + var ret, + target = this, + related = event.relatedTarget, + handleObj = event.handleObj; + + // For mouseenter/leave call the handler if related is outside the target. + // NB: No relatedTarget if the mouse left/entered the browser window + if ( !related || ( related !== target && !jQuery.contains( target, related ) ) ) { + event.type = handleObj.origType; + ret = handleObj.handler.apply( this, arguments ); + event.type = fix; + } + return ret; + } + }; +} ); + +jQuery.fn.extend( { + + on: function( types, selector, data, fn ) { + return on( this, types, selector, data, fn ); + }, + one: function( types, selector, data, fn ) { + return on( this, types, selector, data, fn, 1 ); + }, + off: function( types, selector, fn ) { + var handleObj, type; + if ( types && types.preventDefault && types.handleObj ) { + + // ( event ) dispatched jQuery.Event + handleObj = types.handleObj; + jQuery( types.delegateTarget ).off( + handleObj.namespace ? + handleObj.origType + "." + handleObj.namespace : + handleObj.origType, + handleObj.selector, + handleObj.handler + ); + return this; + } + if ( typeof types === "object" ) { + + // ( types-object [, selector] ) + for ( type in types ) { + this.off( type, selector, types[ type ] ); + } + return this; + } + if ( selector === false || typeof selector === "function" ) { + + // ( types [, fn] ) + fn = selector; + selector = undefined; + } + if ( fn === false ) { + fn = returnFalse; + } + return this.each( function() { + jQuery.event.remove( this, types, fn, selector ); + } ); + } +} ); + + +var + + // Support: IE <=10 - 11, Edge 12 - 13 only + // In IE/Edge using regex groups here causes severe slowdowns. + // See https://connect.microsoft.com/IE/feedback/details/1736512/ + rnoInnerhtml = /\s*$/g; + +// Prefer a tbody over its parent table for containing new rows +function manipulationTarget( elem, content ) { + if ( nodeName( elem, "table" ) && + nodeName( content.nodeType !== 11 ? content : content.firstChild, "tr" ) ) { + + return jQuery( elem ).children( "tbody" )[ 0 ] || elem; + } + + return elem; +} + +// Replace/restore the type attribute of script elements for safe DOM manipulation +function disableScript( elem ) { + elem.type = ( elem.getAttribute( "type" ) !== null ) + "/" + elem.type; + return elem; +} +function restoreScript( elem ) { + if ( ( elem.type || "" ).slice( 0, 5 ) === "true/" ) { + elem.type = elem.type.slice( 5 ); + } else { + elem.removeAttribute( "type" ); + } + + return elem; +} + +function cloneCopyEvent( src, dest ) { + var i, l, type, pdataOld, udataOld, udataCur, events; + + if ( dest.nodeType !== 1 ) { + return; + } + + // 1. Copy private data: events, handlers, etc. + if ( dataPriv.hasData( src ) ) { + pdataOld = dataPriv.get( src ); + events = pdataOld.events; + + if ( events ) { + dataPriv.remove( dest, "handle events" ); + + for ( type in events ) { + for ( i = 0, l = events[ type ].length; i < l; i++ ) { + jQuery.event.add( dest, type, events[ type ][ i ] ); + } + } + } + } + + // 2. Copy user data + if ( dataUser.hasData( src ) ) { + udataOld = dataUser.access( src ); + udataCur = jQuery.extend( {}, udataOld ); + + dataUser.set( dest, udataCur ); + } +} + +// Fix IE bugs, see support tests +function fixInput( src, dest ) { + var nodeName = dest.nodeName.toLowerCase(); + + // Fails to persist the checked state of a cloned checkbox or radio button. + if ( nodeName === "input" && rcheckableType.test( src.type ) ) { + dest.checked = src.checked; + + // Fails to return the selected option to the default selected state when cloning options + } else if ( nodeName === "input" || nodeName === "textarea" ) { + dest.defaultValue = src.defaultValue; + } +} + +function domManip( collection, args, callback, ignored ) { + + // Flatten any nested arrays + args = flat( args ); + + var fragment, first, scripts, hasScripts, node, doc, + i = 0, + l = collection.length, + iNoClone = l - 1, + value = args[ 0 ], + valueIsFunction = isFunction( value ); + + // We can't cloneNode fragments that contain checked, in WebKit + if ( valueIsFunction || + ( l > 1 && typeof value === "string" && + !support.checkClone && rchecked.test( value ) ) ) { + return collection.each( function( index ) { + var self = collection.eq( index ); + if ( valueIsFunction ) { + args[ 0 ] = value.call( this, index, self.html() ); + } + domManip( self, args, callback, ignored ); + } ); + } + + if ( l ) { + fragment = buildFragment( args, collection[ 0 ].ownerDocument, false, collection, ignored ); + first = fragment.firstChild; + + if ( fragment.childNodes.length === 1 ) { + fragment = first; + } + + // Require either new content or an interest in ignored elements to invoke the callback + if ( first || ignored ) { + scripts = jQuery.map( getAll( fragment, "script" ), disableScript ); + hasScripts = scripts.length; + + // Use the original fragment for the last item + // instead of the first because it can end up + // being emptied incorrectly in certain situations (#8070). + for ( ; i < l; i++ ) { + node = fragment; + + if ( i !== iNoClone ) { + node = jQuery.clone( node, true, true ); + + // Keep references to cloned scripts for later restoration + if ( hasScripts ) { + + // Support: Android <=4.0 only, PhantomJS 1 only + // push.apply(_, arraylike) throws on ancient WebKit + jQuery.merge( scripts, getAll( node, "script" ) ); + } + } + + callback.call( collection[ i ], node, i ); + } + + if ( hasScripts ) { + doc = scripts[ scripts.length - 1 ].ownerDocument; + + // Reenable scripts + jQuery.map( scripts, restoreScript ); + + // Evaluate executable scripts on first document insertion + for ( i = 0; i < hasScripts; i++ ) { + node = scripts[ i ]; + if ( rscriptType.test( node.type || "" ) && + !dataPriv.access( node, "globalEval" ) && + jQuery.contains( doc, node ) ) { + + if ( node.src && ( node.type || "" ).toLowerCase() !== "module" ) { + + // Optional AJAX dependency, but won't run scripts if not present + if ( jQuery._evalUrl && !node.noModule ) { + jQuery._evalUrl( node.src, { + nonce: node.nonce || node.getAttribute( "nonce" ) + }, doc ); + } + } else { + DOMEval( node.textContent.replace( rcleanScript, "" ), node, doc ); + } + } + } + } + } + } + + return collection; +} + +function remove( elem, selector, keepData ) { + var node, + nodes = selector ? jQuery.filter( selector, elem ) : elem, + i = 0; + + for ( ; ( node = nodes[ i ] ) != null; i++ ) { + if ( !keepData && node.nodeType === 1 ) { + jQuery.cleanData( getAll( node ) ); + } + + if ( node.parentNode ) { + if ( keepData && isAttached( node ) ) { + setGlobalEval( getAll( node, "script" ) ); + } + node.parentNode.removeChild( node ); + } + } + + return elem; +} + +jQuery.extend( { + htmlPrefilter: function( html ) { + return html; + }, + + clone: function( elem, dataAndEvents, deepDataAndEvents ) { + var i, l, srcElements, destElements, + clone = elem.cloneNode( true ), + inPage = isAttached( elem ); + + // Fix IE cloning issues + if ( !support.noCloneChecked && ( elem.nodeType === 1 || elem.nodeType === 11 ) && + !jQuery.isXMLDoc( elem ) ) { + + // We eschew Sizzle here for performance reasons: https://jsperf.com/getall-vs-sizzle/2 + destElements = getAll( clone ); + srcElements = getAll( elem ); + + for ( i = 0, l = srcElements.length; i < l; i++ ) { + fixInput( srcElements[ i ], destElements[ i ] ); + } + } + + // Copy the events from the original to the clone + if ( dataAndEvents ) { + if ( deepDataAndEvents ) { + srcElements = srcElements || getAll( elem ); + destElements = destElements || getAll( clone ); + + for ( i = 0, l = srcElements.length; i < l; i++ ) { + cloneCopyEvent( srcElements[ i ], destElements[ i ] ); + } + } else { + cloneCopyEvent( elem, clone ); + } + } + + // Preserve script evaluation history + destElements = getAll( clone, "script" ); + if ( destElements.length > 0 ) { + setGlobalEval( destElements, !inPage && getAll( elem, "script" ) ); + } + + // Return the cloned set + return clone; + }, + + cleanData: function( elems ) { + var data, elem, type, + special = jQuery.event.special, + i = 0; + + for ( ; ( elem = elems[ i ] ) !== undefined; i++ ) { + if ( acceptData( elem ) ) { + if ( ( data = elem[ dataPriv.expando ] ) ) { + if ( data.events ) { + for ( type in data.events ) { + if ( special[ type ] ) { + jQuery.event.remove( elem, type ); + + // This is a shortcut to avoid jQuery.event.remove's overhead + } else { + jQuery.removeEvent( elem, type, data.handle ); + } + } + } + + // Support: Chrome <=35 - 45+ + // Assign undefined instead of using delete, see Data#remove + elem[ dataPriv.expando ] = undefined; + } + if ( elem[ dataUser.expando ] ) { + + // Support: Chrome <=35 - 45+ + // Assign undefined instead of using delete, see Data#remove + elem[ dataUser.expando ] = undefined; + } + } + } + } +} ); + +jQuery.fn.extend( { + detach: function( selector ) { + return remove( this, selector, true ); + }, + + remove: function( selector ) { + return remove( this, selector ); + }, + + text: function( value ) { + return access( this, function( value ) { + return value === undefined ? + jQuery.text( this ) : + this.empty().each( function() { + if ( this.nodeType === 1 || this.nodeType === 11 || this.nodeType === 9 ) { + this.textContent = value; + } + } ); + }, null, value, arguments.length ); + }, + + append: function() { + return domManip( this, arguments, function( elem ) { + if ( this.nodeType === 1 || this.nodeType === 11 || this.nodeType === 9 ) { + var target = manipulationTarget( this, elem ); + target.appendChild( elem ); + } + } ); + }, + + prepend: function() { + return domManip( this, arguments, function( elem ) { + if ( this.nodeType === 1 || this.nodeType === 11 || this.nodeType === 9 ) { + var target = manipulationTarget( this, elem ); + target.insertBefore( elem, target.firstChild ); + } + } ); + }, + + before: function() { + return domManip( this, arguments, function( elem ) { + if ( this.parentNode ) { + this.parentNode.insertBefore( elem, this ); + } + } ); + }, + + after: function() { + return domManip( this, arguments, function( elem ) { + if ( this.parentNode ) { + this.parentNode.insertBefore( elem, this.nextSibling ); + } + } ); + }, + + empty: function() { + var elem, + i = 0; + + for ( ; ( elem = this[ i ] ) != null; i++ ) { + if ( elem.nodeType === 1 ) { + + // Prevent memory leaks + jQuery.cleanData( getAll( elem, false ) ); + + // Remove any remaining nodes + elem.textContent = ""; + } + } + + return this; + }, + + clone: function( dataAndEvents, deepDataAndEvents ) { + dataAndEvents = dataAndEvents == null ? false : dataAndEvents; + deepDataAndEvents = deepDataAndEvents == null ? dataAndEvents : deepDataAndEvents; + + return this.map( function() { + return jQuery.clone( this, dataAndEvents, deepDataAndEvents ); + } ); + }, + + html: function( value ) { + return access( this, function( value ) { + var elem = this[ 0 ] || {}, + i = 0, + l = this.length; + + if ( value === undefined && elem.nodeType === 1 ) { + return elem.innerHTML; + } + + // See if we can take a shortcut and just use innerHTML + if ( typeof value === "string" && !rnoInnerhtml.test( value ) && + !wrapMap[ ( rtagName.exec( value ) || [ "", "" ] )[ 1 ].toLowerCase() ] ) { + + value = jQuery.htmlPrefilter( value ); + + try { + for ( ; i < l; i++ ) { + elem = this[ i ] || {}; + + // Remove element nodes and prevent memory leaks + if ( elem.nodeType === 1 ) { + jQuery.cleanData( getAll( elem, false ) ); + elem.innerHTML = value; + } + } + + elem = 0; + + // If using innerHTML throws an exception, use the fallback method + } catch ( e ) {} + } + + if ( elem ) { + this.empty().append( value ); + } + }, null, value, arguments.length ); + }, + + replaceWith: function() { + var ignored = []; + + // Make the changes, replacing each non-ignored context element with the new content + return domManip( this, arguments, function( elem ) { + var parent = this.parentNode; + + if ( jQuery.inArray( this, ignored ) < 0 ) { + jQuery.cleanData( getAll( this ) ); + if ( parent ) { + parent.replaceChild( elem, this ); + } + } + + // Force callback invocation + }, ignored ); + } +} ); + +jQuery.each( { + appendTo: "append", + prependTo: "prepend", + insertBefore: "before", + insertAfter: "after", + replaceAll: "replaceWith" +}, function( name, original ) { + jQuery.fn[ name ] = function( selector ) { + var elems, + ret = [], + insert = jQuery( selector ), + last = insert.length - 1, + i = 0; + + for ( ; i <= last; i++ ) { + elems = i === last ? this : this.clone( true ); + jQuery( insert[ i ] )[ original ]( elems ); + + // Support: Android <=4.0 only, PhantomJS 1 only + // .get() because push.apply(_, arraylike) throws on ancient WebKit + push.apply( ret, elems.get() ); + } + + return this.pushStack( ret ); + }; +} ); +var rnumnonpx = new RegExp( "^(" + pnum + ")(?!px)[a-z%]+$", "i" ); + +var getStyles = function( elem ) { + + // Support: IE <=11 only, Firefox <=30 (#15098, #14150) + // IE throws on elements created in popups + // FF meanwhile throws on frame elements through "defaultView.getComputedStyle" + var view = elem.ownerDocument.defaultView; + + if ( !view || !view.opener ) { + view = window; + } + + return view.getComputedStyle( elem ); + }; + +var swap = function( elem, options, callback ) { + var ret, name, + old = {}; + + // Remember the old values, and insert the new ones + for ( name in options ) { + old[ name ] = elem.style[ name ]; + elem.style[ name ] = options[ name ]; + } + + ret = callback.call( elem ); + + // Revert the old values + for ( name in options ) { + elem.style[ name ] = old[ name ]; + } + + return ret; +}; + + +var rboxStyle = new RegExp( cssExpand.join( "|" ), "i" ); + + + +( function() { + + // Executing both pixelPosition & boxSizingReliable tests require only one layout + // so they're executed at the same time to save the second computation. + function computeStyleTests() { + + // This is a singleton, we need to execute it only once + if ( !div ) { + return; + } + + container.style.cssText = "position:absolute;left:-11111px;width:60px;" + + "margin-top:1px;padding:0;border:0"; + div.style.cssText = + "position:relative;display:block;box-sizing:border-box;overflow:scroll;" + + "margin:auto;border:1px;padding:1px;" + + "width:60%;top:1%"; + documentElement.appendChild( container ).appendChild( div ); + + var divStyle = window.getComputedStyle( div ); + pixelPositionVal = divStyle.top !== "1%"; + + // Support: Android 4.0 - 4.3 only, Firefox <=3 - 44 + reliableMarginLeftVal = roundPixelMeasures( divStyle.marginLeft ) === 12; + + // Support: Android 4.0 - 4.3 only, Safari <=9.1 - 10.1, iOS <=7.0 - 9.3 + // Some styles come back with percentage values, even though they shouldn't + div.style.right = "60%"; + pixelBoxStylesVal = roundPixelMeasures( divStyle.right ) === 36; + + // Support: IE 9 - 11 only + // Detect misreporting of content dimensions for box-sizing:border-box elements + boxSizingReliableVal = roundPixelMeasures( divStyle.width ) === 36; + + // Support: IE 9 only + // Detect overflow:scroll screwiness (gh-3699) + // Support: Chrome <=64 + // Don't get tricked when zoom affects offsetWidth (gh-4029) + div.style.position = "absolute"; + scrollboxSizeVal = roundPixelMeasures( div.offsetWidth / 3 ) === 12; + + documentElement.removeChild( container ); + + // Nullify the div so it wouldn't be stored in the memory and + // it will also be a sign that checks already performed + div = null; + } + + function roundPixelMeasures( measure ) { + return Math.round( parseFloat( measure ) ); + } + + var pixelPositionVal, boxSizingReliableVal, scrollboxSizeVal, pixelBoxStylesVal, + reliableTrDimensionsVal, reliableMarginLeftVal, + container = document.createElement( "div" ), + div = document.createElement( "div" ); + + // Finish early in limited (non-browser) environments + if ( !div.style ) { + return; + } + + // Support: IE <=9 - 11 only + // Style of cloned element affects source element cloned (#8908) + div.style.backgroundClip = "content-box"; + div.cloneNode( true ).style.backgroundClip = ""; + support.clearCloneStyle = div.style.backgroundClip === "content-box"; + + jQuery.extend( support, { + boxSizingReliable: function() { + computeStyleTests(); + return boxSizingReliableVal; + }, + pixelBoxStyles: function() { + computeStyleTests(); + return pixelBoxStylesVal; + }, + pixelPosition: function() { + computeStyleTests(); + return pixelPositionVal; + }, + reliableMarginLeft: function() { + computeStyleTests(); + return reliableMarginLeftVal; + }, + scrollboxSize: function() { + computeStyleTests(); + return scrollboxSizeVal; + }, + + // Support: IE 9 - 11+, Edge 15 - 18+ + // IE/Edge misreport `getComputedStyle` of table rows with width/height + // set in CSS while `offset*` properties report correct values. + // Behavior in IE 9 is more subtle than in newer versions & it passes + // some versions of this test; make sure not to make it pass there! + // + // Support: Firefox 70+ + // Only Firefox includes border widths + // in computed dimensions. (gh-4529) + reliableTrDimensions: function() { + var table, tr, trChild, trStyle; + if ( reliableTrDimensionsVal == null ) { + table = document.createElement( "table" ); + tr = document.createElement( "tr" ); + trChild = document.createElement( "div" ); + + table.style.cssText = "position:absolute;left:-11111px;border-collapse:separate"; + tr.style.cssText = "border:1px solid"; + + // Support: Chrome 86+ + // Height set through cssText does not get applied. + // Computed height then comes back as 0. + tr.style.height = "1px"; + trChild.style.height = "9px"; + + // Support: Android 8 Chrome 86+ + // In our bodyBackground.html iframe, + // display for all div elements is set to "inline", + // which causes a problem only in Android 8 Chrome 86. + // Ensuring the div is display: block + // gets around this issue. + trChild.style.display = "block"; + + documentElement + .appendChild( table ) + .appendChild( tr ) + .appendChild( trChild ); + + trStyle = window.getComputedStyle( tr ); + reliableTrDimensionsVal = ( parseInt( trStyle.height, 10 ) + + parseInt( trStyle.borderTopWidth, 10 ) + + parseInt( trStyle.borderBottomWidth, 10 ) ) === tr.offsetHeight; + + documentElement.removeChild( table ); + } + return reliableTrDimensionsVal; + } + } ); +} )(); + + +function curCSS( elem, name, computed ) { + var width, minWidth, maxWidth, ret, + + // Support: Firefox 51+ + // Retrieving style before computed somehow + // fixes an issue with getting wrong values + // on detached elements + style = elem.style; + + computed = computed || getStyles( elem ); + + // getPropertyValue is needed for: + // .css('filter') (IE 9 only, #12537) + // .css('--customProperty) (#3144) + if ( computed ) { + ret = computed.getPropertyValue( name ) || computed[ name ]; + + if ( ret === "" && !isAttached( elem ) ) { + ret = jQuery.style( elem, name ); + } + + // A tribute to the "awesome hack by Dean Edwards" + // Android Browser returns percentage for some values, + // but width seems to be reliably pixels. + // This is against the CSSOM draft spec: + // https://drafts.csswg.org/cssom/#resolved-values + if ( !support.pixelBoxStyles() && rnumnonpx.test( ret ) && rboxStyle.test( name ) ) { + + // Remember the original values + width = style.width; + minWidth = style.minWidth; + maxWidth = style.maxWidth; + + // Put in the new values to get a computed value out + style.minWidth = style.maxWidth = style.width = ret; + ret = computed.width; + + // Revert the changed values + style.width = width; + style.minWidth = minWidth; + style.maxWidth = maxWidth; + } + } + + return ret !== undefined ? + + // Support: IE <=9 - 11 only + // IE returns zIndex value as an integer. + ret + "" : + ret; +} + + +function addGetHookIf( conditionFn, hookFn ) { + + // Define the hook, we'll check on the first run if it's really needed. + return { + get: function() { + if ( conditionFn() ) { + + // Hook not needed (or it's not possible to use it due + // to missing dependency), remove it. + delete this.get; + return; + } + + // Hook needed; redefine it so that the support test is not executed again. + return ( this.get = hookFn ).apply( this, arguments ); + } + }; +} + + +var cssPrefixes = [ "Webkit", "Moz", "ms" ], + emptyStyle = document.createElement( "div" ).style, + vendorProps = {}; + +// Return a vendor-prefixed property or undefined +function vendorPropName( name ) { + + // Check for vendor prefixed names + var capName = name[ 0 ].toUpperCase() + name.slice( 1 ), + i = cssPrefixes.length; + + while ( i-- ) { + name = cssPrefixes[ i ] + capName; + if ( name in emptyStyle ) { + return name; + } + } +} + +// Return a potentially-mapped jQuery.cssProps or vendor prefixed property +function finalPropName( name ) { + var final = jQuery.cssProps[ name ] || vendorProps[ name ]; + + if ( final ) { + return final; + } + if ( name in emptyStyle ) { + return name; + } + return vendorProps[ name ] = vendorPropName( name ) || name; +} + + +var + + // Swappable if display is none or starts with table + // except "table", "table-cell", or "table-caption" + // See here for display values: https://developer.mozilla.org/en-US/docs/CSS/display + rdisplayswap = /^(none|table(?!-c[ea]).+)/, + rcustomProp = /^--/, + cssShow = { position: "absolute", visibility: "hidden", display: "block" }, + cssNormalTransform = { + letterSpacing: "0", + fontWeight: "400" + }; + +function setPositiveNumber( _elem, value, subtract ) { + + // Any relative (+/-) values have already been + // normalized at this point + var matches = rcssNum.exec( value ); + return matches ? + + // Guard against undefined "subtract", e.g., when used as in cssHooks + Math.max( 0, matches[ 2 ] - ( subtract || 0 ) ) + ( matches[ 3 ] || "px" ) : + value; +} + +function boxModelAdjustment( elem, dimension, box, isBorderBox, styles, computedVal ) { + var i = dimension === "width" ? 1 : 0, + extra = 0, + delta = 0; + + // Adjustment may not be necessary + if ( box === ( isBorderBox ? "border" : "content" ) ) { + return 0; + } + + for ( ; i < 4; i += 2 ) { + + // Both box models exclude margin + if ( box === "margin" ) { + delta += jQuery.css( elem, box + cssExpand[ i ], true, styles ); + } + + // If we get here with a content-box, we're seeking "padding" or "border" or "margin" + if ( !isBorderBox ) { + + // Add padding + delta += jQuery.css( elem, "padding" + cssExpand[ i ], true, styles ); + + // For "border" or "margin", add border + if ( box !== "padding" ) { + delta += jQuery.css( elem, "border" + cssExpand[ i ] + "Width", true, styles ); + + // But still keep track of it otherwise + } else { + extra += jQuery.css( elem, "border" + cssExpand[ i ] + "Width", true, styles ); + } + + // If we get here with a border-box (content + padding + border), we're seeking "content" or + // "padding" or "margin" + } else { + + // For "content", subtract padding + if ( box === "content" ) { + delta -= jQuery.css( elem, "padding" + cssExpand[ i ], true, styles ); + } + + // For "content" or "padding", subtract border + if ( box !== "margin" ) { + delta -= jQuery.css( elem, "border" + cssExpand[ i ] + "Width", true, styles ); + } + } + } + + // Account for positive content-box scroll gutter when requested by providing computedVal + if ( !isBorderBox && computedVal >= 0 ) { + + // offsetWidth/offsetHeight is a rounded sum of content, padding, scroll gutter, and border + // Assuming integer scroll gutter, subtract the rest and round down + delta += Math.max( 0, Math.ceil( + elem[ "offset" + dimension[ 0 ].toUpperCase() + dimension.slice( 1 ) ] - + computedVal - + delta - + extra - + 0.5 + + // If offsetWidth/offsetHeight is unknown, then we can't determine content-box scroll gutter + // Use an explicit zero to avoid NaN (gh-3964) + ) ) || 0; + } + + return delta; +} + +function getWidthOrHeight( elem, dimension, extra ) { + + // Start with computed style + var styles = getStyles( elem ), + + // To avoid forcing a reflow, only fetch boxSizing if we need it (gh-4322). + // Fake content-box until we know it's needed to know the true value. + boxSizingNeeded = !support.boxSizingReliable() || extra, + isBorderBox = boxSizingNeeded && + jQuery.css( elem, "boxSizing", false, styles ) === "border-box", + valueIsBorderBox = isBorderBox, + + val = curCSS( elem, dimension, styles ), + offsetProp = "offset" + dimension[ 0 ].toUpperCase() + dimension.slice( 1 ); + + // Support: Firefox <=54 + // Return a confounding non-pixel value or feign ignorance, as appropriate. + if ( rnumnonpx.test( val ) ) { + if ( !extra ) { + return val; + } + val = "auto"; + } + + + // Support: IE 9 - 11 only + // Use offsetWidth/offsetHeight for when box sizing is unreliable. + // In those cases, the computed value can be trusted to be border-box. + if ( ( !support.boxSizingReliable() && isBorderBox || + + // Support: IE 10 - 11+, Edge 15 - 18+ + // IE/Edge misreport `getComputedStyle` of table rows with width/height + // set in CSS while `offset*` properties report correct values. + // Interestingly, in some cases IE 9 doesn't suffer from this issue. + !support.reliableTrDimensions() && nodeName( elem, "tr" ) || + + // Fall back to offsetWidth/offsetHeight when value is "auto" + // This happens for inline elements with no explicit setting (gh-3571) + val === "auto" || + + // Support: Android <=4.1 - 4.3 only + // Also use offsetWidth/offsetHeight for misreported inline dimensions (gh-3602) + !parseFloat( val ) && jQuery.css( elem, "display", false, styles ) === "inline" ) && + + // Make sure the element is visible & connected + elem.getClientRects().length ) { + + isBorderBox = jQuery.css( elem, "boxSizing", false, styles ) === "border-box"; + + // Where available, offsetWidth/offsetHeight approximate border box dimensions. + // Where not available (e.g., SVG), assume unreliable box-sizing and interpret the + // retrieved value as a content box dimension. + valueIsBorderBox = offsetProp in elem; + if ( valueIsBorderBox ) { + val = elem[ offsetProp ]; + } + } + + // Normalize "" and auto + val = parseFloat( val ) || 0; + + // Adjust for the element's box model + return ( val + + boxModelAdjustment( + elem, + dimension, + extra || ( isBorderBox ? "border" : "content" ), + valueIsBorderBox, + styles, + + // Provide the current computed size to request scroll gutter calculation (gh-3589) + val + ) + ) + "px"; +} + +jQuery.extend( { + + // Add in style property hooks for overriding the default + // behavior of getting and setting a style property + cssHooks: { + opacity: { + get: function( elem, computed ) { + if ( computed ) { + + // We should always get a number back from opacity + var ret = curCSS( elem, "opacity" ); + return ret === "" ? "1" : ret; + } + } + } + }, + + // Don't automatically add "px" to these possibly-unitless properties + cssNumber: { + "animationIterationCount": true, + "columnCount": true, + "fillOpacity": true, + "flexGrow": true, + "flexShrink": true, + "fontWeight": true, + "gridArea": true, + "gridColumn": true, + "gridColumnEnd": true, + "gridColumnStart": true, + "gridRow": true, + "gridRowEnd": true, + "gridRowStart": true, + "lineHeight": true, + "opacity": true, + "order": true, + "orphans": true, + "widows": true, + "zIndex": true, + "zoom": true + }, + + // Add in properties whose names you wish to fix before + // setting or getting the value + cssProps: {}, + + // Get and set the style property on a DOM Node + style: function( elem, name, value, extra ) { + + // Don't set styles on text and comment nodes + if ( !elem || elem.nodeType === 3 || elem.nodeType === 8 || !elem.style ) { + return; + } + + // Make sure that we're working with the right name + var ret, type, hooks, + origName = camelCase( name ), + isCustomProp = rcustomProp.test( name ), + style = elem.style; + + // Make sure that we're working with the right name. We don't + // want to query the value if it is a CSS custom property + // since they are user-defined. + if ( !isCustomProp ) { + name = finalPropName( origName ); + } + + // Gets hook for the prefixed version, then unprefixed version + hooks = jQuery.cssHooks[ name ] || jQuery.cssHooks[ origName ]; + + // Check if we're setting a value + if ( value !== undefined ) { + type = typeof value; + + // Convert "+=" or "-=" to relative numbers (#7345) + if ( type === "string" && ( ret = rcssNum.exec( value ) ) && ret[ 1 ] ) { + value = adjustCSS( elem, name, ret ); + + // Fixes bug #9237 + type = "number"; + } + + // Make sure that null and NaN values aren't set (#7116) + if ( value == null || value !== value ) { + return; + } + + // If a number was passed in, add the unit (except for certain CSS properties) + // The isCustomProp check can be removed in jQuery 4.0 when we only auto-append + // "px" to a few hardcoded values. + if ( type === "number" && !isCustomProp ) { + value += ret && ret[ 3 ] || ( jQuery.cssNumber[ origName ] ? "" : "px" ); + } + + // background-* props affect original clone's values + if ( !support.clearCloneStyle && value === "" && name.indexOf( "background" ) === 0 ) { + style[ name ] = "inherit"; + } + + // If a hook was provided, use that value, otherwise just set the specified value + if ( !hooks || !( "set" in hooks ) || + ( value = hooks.set( elem, value, extra ) ) !== undefined ) { + + if ( isCustomProp ) { + style.setProperty( name, value ); + } else { + style[ name ] = value; + } + } + + } else { + + // If a hook was provided get the non-computed value from there + if ( hooks && "get" in hooks && + ( ret = hooks.get( elem, false, extra ) ) !== undefined ) { + + return ret; + } + + // Otherwise just get the value from the style object + return style[ name ]; + } + }, + + css: function( elem, name, extra, styles ) { + var val, num, hooks, + origName = camelCase( name ), + isCustomProp = rcustomProp.test( name ); + + // Make sure that we're working with the right name. We don't + // want to modify the value if it is a CSS custom property + // since they are user-defined. + if ( !isCustomProp ) { + name = finalPropName( origName ); + } + + // Try prefixed name followed by the unprefixed name + hooks = jQuery.cssHooks[ name ] || jQuery.cssHooks[ origName ]; + + // If a hook was provided get the computed value from there + if ( hooks && "get" in hooks ) { + val = hooks.get( elem, true, extra ); + } + + // Otherwise, if a way to get the computed value exists, use that + if ( val === undefined ) { + val = curCSS( elem, name, styles ); + } + + // Convert "normal" to computed value + if ( val === "normal" && name in cssNormalTransform ) { + val = cssNormalTransform[ name ]; + } + + // Make numeric if forced or a qualifier was provided and val looks numeric + if ( extra === "" || extra ) { + num = parseFloat( val ); + return extra === true || isFinite( num ) ? num || 0 : val; + } + + return val; + } +} ); + +jQuery.each( [ "height", "width" ], function( _i, dimension ) { + jQuery.cssHooks[ dimension ] = { + get: function( elem, computed, extra ) { + if ( computed ) { + + // Certain elements can have dimension info if we invisibly show them + // but it must have a current display style that would benefit + return rdisplayswap.test( jQuery.css( elem, "display" ) ) && + + // Support: Safari 8+ + // Table columns in Safari have non-zero offsetWidth & zero + // getBoundingClientRect().width unless display is changed. + // Support: IE <=11 only + // Running getBoundingClientRect on a disconnected node + // in IE throws an error. + ( !elem.getClientRects().length || !elem.getBoundingClientRect().width ) ? + swap( elem, cssShow, function() { + return getWidthOrHeight( elem, dimension, extra ); + } ) : + getWidthOrHeight( elem, dimension, extra ); + } + }, + + set: function( elem, value, extra ) { + var matches, + styles = getStyles( elem ), + + // Only read styles.position if the test has a chance to fail + // to avoid forcing a reflow. + scrollboxSizeBuggy = !support.scrollboxSize() && + styles.position === "absolute", + + // To avoid forcing a reflow, only fetch boxSizing if we need it (gh-3991) + boxSizingNeeded = scrollboxSizeBuggy || extra, + isBorderBox = boxSizingNeeded && + jQuery.css( elem, "boxSizing", false, styles ) === "border-box", + subtract = extra ? + boxModelAdjustment( + elem, + dimension, + extra, + isBorderBox, + styles + ) : + 0; + + // Account for unreliable border-box dimensions by comparing offset* to computed and + // faking a content-box to get border and padding (gh-3699) + if ( isBorderBox && scrollboxSizeBuggy ) { + subtract -= Math.ceil( + elem[ "offset" + dimension[ 0 ].toUpperCase() + dimension.slice( 1 ) ] - + parseFloat( styles[ dimension ] ) - + boxModelAdjustment( elem, dimension, "border", false, styles ) - + 0.5 + ); + } + + // Convert to pixels if value adjustment is needed + if ( subtract && ( matches = rcssNum.exec( value ) ) && + ( matches[ 3 ] || "px" ) !== "px" ) { + + elem.style[ dimension ] = value; + value = jQuery.css( elem, dimension ); + } + + return setPositiveNumber( elem, value, subtract ); + } + }; +} ); + +jQuery.cssHooks.marginLeft = addGetHookIf( support.reliableMarginLeft, + function( elem, computed ) { + if ( computed ) { + return ( parseFloat( curCSS( elem, "marginLeft" ) ) || + elem.getBoundingClientRect().left - + swap( elem, { marginLeft: 0 }, function() { + return elem.getBoundingClientRect().left; + } ) + ) + "px"; + } + } +); + +// These hooks are used by animate to expand properties +jQuery.each( { + margin: "", + padding: "", + border: "Width" +}, function( prefix, suffix ) { + jQuery.cssHooks[ prefix + suffix ] = { + expand: function( value ) { + var i = 0, + expanded = {}, + + // Assumes a single number if not a string + parts = typeof value === "string" ? value.split( " " ) : [ value ]; + + for ( ; i < 4; i++ ) { + expanded[ prefix + cssExpand[ i ] + suffix ] = + parts[ i ] || parts[ i - 2 ] || parts[ 0 ]; + } + + return expanded; + } + }; + + if ( prefix !== "margin" ) { + jQuery.cssHooks[ prefix + suffix ].set = setPositiveNumber; + } +} ); + +jQuery.fn.extend( { + css: function( name, value ) { + return access( this, function( elem, name, value ) { + var styles, len, + map = {}, + i = 0; + + if ( Array.isArray( name ) ) { + styles = getStyles( elem ); + len = name.length; + + for ( ; i < len; i++ ) { + map[ name[ i ] ] = jQuery.css( elem, name[ i ], false, styles ); + } + + return map; + } + + return value !== undefined ? + jQuery.style( elem, name, value ) : + jQuery.css( elem, name ); + }, name, value, arguments.length > 1 ); + } +} ); + + +function Tween( elem, options, prop, end, easing ) { + return new Tween.prototype.init( elem, options, prop, end, easing ); +} +jQuery.Tween = Tween; + +Tween.prototype = { + constructor: Tween, + init: function( elem, options, prop, end, easing, unit ) { + this.elem = elem; + this.prop = prop; + this.easing = easing || jQuery.easing._default; + this.options = options; + this.start = this.now = this.cur(); + this.end = end; + this.unit = unit || ( jQuery.cssNumber[ prop ] ? "" : "px" ); + }, + cur: function() { + var hooks = Tween.propHooks[ this.prop ]; + + return hooks && hooks.get ? + hooks.get( this ) : + Tween.propHooks._default.get( this ); + }, + run: function( percent ) { + var eased, + hooks = Tween.propHooks[ this.prop ]; + + if ( this.options.duration ) { + this.pos = eased = jQuery.easing[ this.easing ]( + percent, this.options.duration * percent, 0, 1, this.options.duration + ); + } else { + this.pos = eased = percent; + } + this.now = ( this.end - this.start ) * eased + this.start; + + if ( this.options.step ) { + this.options.step.call( this.elem, this.now, this ); + } + + if ( hooks && hooks.set ) { + hooks.set( this ); + } else { + Tween.propHooks._default.set( this ); + } + return this; + } +}; + +Tween.prototype.init.prototype = Tween.prototype; + +Tween.propHooks = { + _default: { + get: function( tween ) { + var result; + + // Use a property on the element directly when it is not a DOM element, + // or when there is no matching style property that exists. + if ( tween.elem.nodeType !== 1 || + tween.elem[ tween.prop ] != null && tween.elem.style[ tween.prop ] == null ) { + return tween.elem[ tween.prop ]; + } + + // Passing an empty string as a 3rd parameter to .css will automatically + // attempt a parseFloat and fallback to a string if the parse fails. + // Simple values such as "10px" are parsed to Float; + // complex values such as "rotate(1rad)" are returned as-is. + result = jQuery.css( tween.elem, tween.prop, "" ); + + // Empty strings, null, undefined and "auto" are converted to 0. + return !result || result === "auto" ? 0 : result; + }, + set: function( tween ) { + + // Use step hook for back compat. + // Use cssHook if its there. + // Use .style if available and use plain properties where available. + if ( jQuery.fx.step[ tween.prop ] ) { + jQuery.fx.step[ tween.prop ]( tween ); + } else if ( tween.elem.nodeType === 1 && ( + jQuery.cssHooks[ tween.prop ] || + tween.elem.style[ finalPropName( tween.prop ) ] != null ) ) { + jQuery.style( tween.elem, tween.prop, tween.now + tween.unit ); + } else { + tween.elem[ tween.prop ] = tween.now; + } + } + } +}; + +// Support: IE <=9 only +// Panic based approach to setting things on disconnected nodes +Tween.propHooks.scrollTop = Tween.propHooks.scrollLeft = { + set: function( tween ) { + if ( tween.elem.nodeType && tween.elem.parentNode ) { + tween.elem[ tween.prop ] = tween.now; + } + } +}; + +jQuery.easing = { + linear: function( p ) { + return p; + }, + swing: function( p ) { + return 0.5 - Math.cos( p * Math.PI ) / 2; + }, + _default: "swing" +}; + +jQuery.fx = Tween.prototype.init; + +// Back compat <1.8 extension point +jQuery.fx.step = {}; + + + + +var + fxNow, inProgress, + rfxtypes = /^(?:toggle|show|hide)$/, + rrun = /queueHooks$/; + +function schedule() { + if ( inProgress ) { + if ( document.hidden === false && window.requestAnimationFrame ) { + window.requestAnimationFrame( schedule ); + } else { + window.setTimeout( schedule, jQuery.fx.interval ); + } + + jQuery.fx.tick(); + } +} + +// Animations created synchronously will run synchronously +function createFxNow() { + window.setTimeout( function() { + fxNow = undefined; + } ); + return ( fxNow = Date.now() ); +} + +// Generate parameters to create a standard animation +function genFx( type, includeWidth ) { + var which, + i = 0, + attrs = { height: type }; + + // If we include width, step value is 1 to do all cssExpand values, + // otherwise step value is 2 to skip over Left and Right + includeWidth = includeWidth ? 1 : 0; + for ( ; i < 4; i += 2 - includeWidth ) { + which = cssExpand[ i ]; + attrs[ "margin" + which ] = attrs[ "padding" + which ] = type; + } + + if ( includeWidth ) { + attrs.opacity = attrs.width = type; + } + + return attrs; +} + +function createTween( value, prop, animation ) { + var tween, + collection = ( Animation.tweeners[ prop ] || [] ).concat( Animation.tweeners[ "*" ] ), + index = 0, + length = collection.length; + for ( ; index < length; index++ ) { + if ( ( tween = collection[ index ].call( animation, prop, value ) ) ) { + + // We're done with this property + return tween; + } + } +} + +function defaultPrefilter( elem, props, opts ) { + var prop, value, toggle, hooks, oldfire, propTween, restoreDisplay, display, + isBox = "width" in props || "height" in props, + anim = this, + orig = {}, + style = elem.style, + hidden = elem.nodeType && isHiddenWithinTree( elem ), + dataShow = dataPriv.get( elem, "fxshow" ); + + // Queue-skipping animations hijack the fx hooks + if ( !opts.queue ) { + hooks = jQuery._queueHooks( elem, "fx" ); + if ( hooks.unqueued == null ) { + hooks.unqueued = 0; + oldfire = hooks.empty.fire; + hooks.empty.fire = function() { + if ( !hooks.unqueued ) { + oldfire(); + } + }; + } + hooks.unqueued++; + + anim.always( function() { + + // Ensure the complete handler is called before this completes + anim.always( function() { + hooks.unqueued--; + if ( !jQuery.queue( elem, "fx" ).length ) { + hooks.empty.fire(); + } + } ); + } ); + } + + // Detect show/hide animations + for ( prop in props ) { + value = props[ prop ]; + if ( rfxtypes.test( value ) ) { + delete props[ prop ]; + toggle = toggle || value === "toggle"; + if ( value === ( hidden ? "hide" : "show" ) ) { + + // Pretend to be hidden if this is a "show" and + // there is still data from a stopped show/hide + if ( value === "show" && dataShow && dataShow[ prop ] !== undefined ) { + hidden = true; + + // Ignore all other no-op show/hide data + } else { + continue; + } + } + orig[ prop ] = dataShow && dataShow[ prop ] || jQuery.style( elem, prop ); + } + } + + // Bail out if this is a no-op like .hide().hide() + propTween = !jQuery.isEmptyObject( props ); + if ( !propTween && jQuery.isEmptyObject( orig ) ) { + return; + } + + // Restrict "overflow" and "display" styles during box animations + if ( isBox && elem.nodeType === 1 ) { + + // Support: IE <=9 - 11, Edge 12 - 15 + // Record all 3 overflow attributes because IE does not infer the shorthand + // from identically-valued overflowX and overflowY and Edge just mirrors + // the overflowX value there. + opts.overflow = [ style.overflow, style.overflowX, style.overflowY ]; + + // Identify a display type, preferring old show/hide data over the CSS cascade + restoreDisplay = dataShow && dataShow.display; + if ( restoreDisplay == null ) { + restoreDisplay = dataPriv.get( elem, "display" ); + } + display = jQuery.css( elem, "display" ); + if ( display === "none" ) { + if ( restoreDisplay ) { + display = restoreDisplay; + } else { + + // Get nonempty value(s) by temporarily forcing visibility + showHide( [ elem ], true ); + restoreDisplay = elem.style.display || restoreDisplay; + display = jQuery.css( elem, "display" ); + showHide( [ elem ] ); + } + } + + // Animate inline elements as inline-block + if ( display === "inline" || display === "inline-block" && restoreDisplay != null ) { + if ( jQuery.css( elem, "float" ) === "none" ) { + + // Restore the original display value at the end of pure show/hide animations + if ( !propTween ) { + anim.done( function() { + style.display = restoreDisplay; + } ); + if ( restoreDisplay == null ) { + display = style.display; + restoreDisplay = display === "none" ? "" : display; + } + } + style.display = "inline-block"; + } + } + } + + if ( opts.overflow ) { + style.overflow = "hidden"; + anim.always( function() { + style.overflow = opts.overflow[ 0 ]; + style.overflowX = opts.overflow[ 1 ]; + style.overflowY = opts.overflow[ 2 ]; + } ); + } + + // Implement show/hide animations + propTween = false; + for ( prop in orig ) { + + // General show/hide setup for this element animation + if ( !propTween ) { + if ( dataShow ) { + if ( "hidden" in dataShow ) { + hidden = dataShow.hidden; + } + } else { + dataShow = dataPriv.access( elem, "fxshow", { display: restoreDisplay } ); + } + + // Store hidden/visible for toggle so `.stop().toggle()` "reverses" + if ( toggle ) { + dataShow.hidden = !hidden; + } + + // Show elements before animating them + if ( hidden ) { + showHide( [ elem ], true ); + } + + /* eslint-disable no-loop-func */ + + anim.done( function() { + + /* eslint-enable no-loop-func */ + + // The final step of a "hide" animation is actually hiding the element + if ( !hidden ) { + showHide( [ elem ] ); + } + dataPriv.remove( elem, "fxshow" ); + for ( prop in orig ) { + jQuery.style( elem, prop, orig[ prop ] ); + } + } ); + } + + // Per-property setup + propTween = createTween( hidden ? dataShow[ prop ] : 0, prop, anim ); + if ( !( prop in dataShow ) ) { + dataShow[ prop ] = propTween.start; + if ( hidden ) { + propTween.end = propTween.start; + propTween.start = 0; + } + } + } +} + +function propFilter( props, specialEasing ) { + var index, name, easing, value, hooks; + + // camelCase, specialEasing and expand cssHook pass + for ( index in props ) { + name = camelCase( index ); + easing = specialEasing[ name ]; + value = props[ index ]; + if ( Array.isArray( value ) ) { + easing = value[ 1 ]; + value = props[ index ] = value[ 0 ]; + } + + if ( index !== name ) { + props[ name ] = value; + delete props[ index ]; + } + + hooks = jQuery.cssHooks[ name ]; + if ( hooks && "expand" in hooks ) { + value = hooks.expand( value ); + delete props[ name ]; + + // Not quite $.extend, this won't overwrite existing keys. + // Reusing 'index' because we have the correct "name" + for ( index in value ) { + if ( !( index in props ) ) { + props[ index ] = value[ index ]; + specialEasing[ index ] = easing; + } + } + } else { + specialEasing[ name ] = easing; + } + } +} + +function Animation( elem, properties, options ) { + var result, + stopped, + index = 0, + length = Animation.prefilters.length, + deferred = jQuery.Deferred().always( function() { + + // Don't match elem in the :animated selector + delete tick.elem; + } ), + tick = function() { + if ( stopped ) { + return false; + } + var currentTime = fxNow || createFxNow(), + remaining = Math.max( 0, animation.startTime + animation.duration - currentTime ), + + // Support: Android 2.3 only + // Archaic crash bug won't allow us to use `1 - ( 0.5 || 0 )` (#12497) + temp = remaining / animation.duration || 0, + percent = 1 - temp, + index = 0, + length = animation.tweens.length; + + for ( ; index < length; index++ ) { + animation.tweens[ index ].run( percent ); + } + + deferred.notifyWith( elem, [ animation, percent, remaining ] ); + + // If there's more to do, yield + if ( percent < 1 && length ) { + return remaining; + } + + // If this was an empty animation, synthesize a final progress notification + if ( !length ) { + deferred.notifyWith( elem, [ animation, 1, 0 ] ); + } + + // Resolve the animation and report its conclusion + deferred.resolveWith( elem, [ animation ] ); + return false; + }, + animation = deferred.promise( { + elem: elem, + props: jQuery.extend( {}, properties ), + opts: jQuery.extend( true, { + specialEasing: {}, + easing: jQuery.easing._default + }, options ), + originalProperties: properties, + originalOptions: options, + startTime: fxNow || createFxNow(), + duration: options.duration, + tweens: [], + createTween: function( prop, end ) { + var tween = jQuery.Tween( elem, animation.opts, prop, end, + animation.opts.specialEasing[ prop ] || animation.opts.easing ); + animation.tweens.push( tween ); + return tween; + }, + stop: function( gotoEnd ) { + var index = 0, + + // If we are going to the end, we want to run all the tweens + // otherwise we skip this part + length = gotoEnd ? animation.tweens.length : 0; + if ( stopped ) { + return this; + } + stopped = true; + for ( ; index < length; index++ ) { + animation.tweens[ index ].run( 1 ); + } + + // Resolve when we played the last frame; otherwise, reject + if ( gotoEnd ) { + deferred.notifyWith( elem, [ animation, 1, 0 ] ); + deferred.resolveWith( elem, [ animation, gotoEnd ] ); + } else { + deferred.rejectWith( elem, [ animation, gotoEnd ] ); + } + return this; + } + } ), + props = animation.props; + + propFilter( props, animation.opts.specialEasing ); + + for ( ; index < length; index++ ) { + result = Animation.prefilters[ index ].call( animation, elem, props, animation.opts ); + if ( result ) { + if ( isFunction( result.stop ) ) { + jQuery._queueHooks( animation.elem, animation.opts.queue ).stop = + result.stop.bind( result ); + } + return result; + } + } + + jQuery.map( props, createTween, animation ); + + if ( isFunction( animation.opts.start ) ) { + animation.opts.start.call( elem, animation ); + } + + // Attach callbacks from options + animation + .progress( animation.opts.progress ) + .done( animation.opts.done, animation.opts.complete ) + .fail( animation.opts.fail ) + .always( animation.opts.always ); + + jQuery.fx.timer( + jQuery.extend( tick, { + elem: elem, + anim: animation, + queue: animation.opts.queue + } ) + ); + + return animation; +} + +jQuery.Animation = jQuery.extend( Animation, { + + tweeners: { + "*": [ function( prop, value ) { + var tween = this.createTween( prop, value ); + adjustCSS( tween.elem, prop, rcssNum.exec( value ), tween ); + return tween; + } ] + }, + + tweener: function( props, callback ) { + if ( isFunction( props ) ) { + callback = props; + props = [ "*" ]; + } else { + props = props.match( rnothtmlwhite ); + } + + var prop, + index = 0, + length = props.length; + + for ( ; index < length; index++ ) { + prop = props[ index ]; + Animation.tweeners[ prop ] = Animation.tweeners[ prop ] || []; + Animation.tweeners[ prop ].unshift( callback ); + } + }, + + prefilters: [ defaultPrefilter ], + + prefilter: function( callback, prepend ) { + if ( prepend ) { + Animation.prefilters.unshift( callback ); + } else { + Animation.prefilters.push( callback ); + } + } +} ); + +jQuery.speed = function( speed, easing, fn ) { + var opt = speed && typeof speed === "object" ? jQuery.extend( {}, speed ) : { + complete: fn || !fn && easing || + isFunction( speed ) && speed, + duration: speed, + easing: fn && easing || easing && !isFunction( easing ) && easing + }; + + // Go to the end state if fx are off + if ( jQuery.fx.off ) { + opt.duration = 0; + + } else { + if ( typeof opt.duration !== "number" ) { + if ( opt.duration in jQuery.fx.speeds ) { + opt.duration = jQuery.fx.speeds[ opt.duration ]; + + } else { + opt.duration = jQuery.fx.speeds._default; + } + } + } + + // Normalize opt.queue - true/undefined/null -> "fx" + if ( opt.queue == null || opt.queue === true ) { + opt.queue = "fx"; + } + + // Queueing + opt.old = opt.complete; + + opt.complete = function() { + if ( isFunction( opt.old ) ) { + opt.old.call( this ); + } + + if ( opt.queue ) { + jQuery.dequeue( this, opt.queue ); + } + }; + + return opt; +}; + +jQuery.fn.extend( { + fadeTo: function( speed, to, easing, callback ) { + + // Show any hidden elements after setting opacity to 0 + return this.filter( isHiddenWithinTree ).css( "opacity", 0 ).show() + + // Animate to the value specified + .end().animate( { opacity: to }, speed, easing, callback ); + }, + animate: function( prop, speed, easing, callback ) { + var empty = jQuery.isEmptyObject( prop ), + optall = jQuery.speed( speed, easing, callback ), + doAnimation = function() { + + // Operate on a copy of prop so per-property easing won't be lost + var anim = Animation( this, jQuery.extend( {}, prop ), optall ); + + // Empty animations, or finishing resolves immediately + if ( empty || dataPriv.get( this, "finish" ) ) { + anim.stop( true ); + } + }; + + doAnimation.finish = doAnimation; + + return empty || optall.queue === false ? + this.each( doAnimation ) : + this.queue( optall.queue, doAnimation ); + }, + stop: function( type, clearQueue, gotoEnd ) { + var stopQueue = function( hooks ) { + var stop = hooks.stop; + delete hooks.stop; + stop( gotoEnd ); + }; + + if ( typeof type !== "string" ) { + gotoEnd = clearQueue; + clearQueue = type; + type = undefined; + } + if ( clearQueue ) { + this.queue( type || "fx", [] ); + } + + return this.each( function() { + var dequeue = true, + index = type != null && type + "queueHooks", + timers = jQuery.timers, + data = dataPriv.get( this ); + + if ( index ) { + if ( data[ index ] && data[ index ].stop ) { + stopQueue( data[ index ] ); + } + } else { + for ( index in data ) { + if ( data[ index ] && data[ index ].stop && rrun.test( index ) ) { + stopQueue( data[ index ] ); + } + } + } + + for ( index = timers.length; index--; ) { + if ( timers[ index ].elem === this && + ( type == null || timers[ index ].queue === type ) ) { + + timers[ index ].anim.stop( gotoEnd ); + dequeue = false; + timers.splice( index, 1 ); + } + } + + // Start the next in the queue if the last step wasn't forced. + // Timers currently will call their complete callbacks, which + // will dequeue but only if they were gotoEnd. + if ( dequeue || !gotoEnd ) { + jQuery.dequeue( this, type ); + } + } ); + }, + finish: function( type ) { + if ( type !== false ) { + type = type || "fx"; + } + return this.each( function() { + var index, + data = dataPriv.get( this ), + queue = data[ type + "queue" ], + hooks = data[ type + "queueHooks" ], + timers = jQuery.timers, + length = queue ? queue.length : 0; + + // Enable finishing flag on private data + data.finish = true; + + // Empty the queue first + jQuery.queue( this, type, [] ); + + if ( hooks && hooks.stop ) { + hooks.stop.call( this, true ); + } + + // Look for any active animations, and finish them + for ( index = timers.length; index--; ) { + if ( timers[ index ].elem === this && timers[ index ].queue === type ) { + timers[ index ].anim.stop( true ); + timers.splice( index, 1 ); + } + } + + // Look for any animations in the old queue and finish them + for ( index = 0; index < length; index++ ) { + if ( queue[ index ] && queue[ index ].finish ) { + queue[ index ].finish.call( this ); + } + } + + // Turn off finishing flag + delete data.finish; + } ); + } +} ); + +jQuery.each( [ "toggle", "show", "hide" ], function( _i, name ) { + var cssFn = jQuery.fn[ name ]; + jQuery.fn[ name ] = function( speed, easing, callback ) { + return speed == null || typeof speed === "boolean" ? + cssFn.apply( this, arguments ) : + this.animate( genFx( name, true ), speed, easing, callback ); + }; +} ); + +// Generate shortcuts for custom animations +jQuery.each( { + slideDown: genFx( "show" ), + slideUp: genFx( "hide" ), + slideToggle: genFx( "toggle" ), + fadeIn: { opacity: "show" }, + fadeOut: { opacity: "hide" }, + fadeToggle: { opacity: "toggle" } +}, function( name, props ) { + jQuery.fn[ name ] = function( speed, easing, callback ) { + return this.animate( props, speed, easing, callback ); + }; +} ); + +jQuery.timers = []; +jQuery.fx.tick = function() { + var timer, + i = 0, + timers = jQuery.timers; + + fxNow = Date.now(); + + for ( ; i < timers.length; i++ ) { + timer = timers[ i ]; + + // Run the timer and safely remove it when done (allowing for external removal) + if ( !timer() && timers[ i ] === timer ) { + timers.splice( i--, 1 ); + } + } + + if ( !timers.length ) { + jQuery.fx.stop(); + } + fxNow = undefined; +}; + +jQuery.fx.timer = function( timer ) { + jQuery.timers.push( timer ); + jQuery.fx.start(); +}; + +jQuery.fx.interval = 13; +jQuery.fx.start = function() { + if ( inProgress ) { + return; + } + + inProgress = true; + schedule(); +}; + +jQuery.fx.stop = function() { + inProgress = null; +}; + +jQuery.fx.speeds = { + slow: 600, + fast: 200, + + // Default speed + _default: 400 +}; + + +// Based off of the plugin by Clint Helfers, with permission. +// https://web.archive.org/web/20100324014747/http://blindsignals.com/index.php/2009/07/jquery-delay/ +jQuery.fn.delay = function( time, type ) { + time = jQuery.fx ? jQuery.fx.speeds[ time ] || time : time; + type = type || "fx"; + + return this.queue( type, function( next, hooks ) { + var timeout = window.setTimeout( next, time ); + hooks.stop = function() { + window.clearTimeout( timeout ); + }; + } ); +}; + + +( function() { + var input = document.createElement( "input" ), + select = document.createElement( "select" ), + opt = select.appendChild( document.createElement( "option" ) ); + + input.type = "checkbox"; + + // Support: Android <=4.3 only + // Default value for a checkbox should be "on" + support.checkOn = input.value !== ""; + + // Support: IE <=11 only + // Must access selectedIndex to make default options select + support.optSelected = opt.selected; + + // Support: IE <=11 only + // An input loses its value after becoming a radio + input = document.createElement( "input" ); + input.value = "t"; + input.type = "radio"; + support.radioValue = input.value === "t"; +} )(); + + +var boolHook, + attrHandle = jQuery.expr.attrHandle; + +jQuery.fn.extend( { + attr: function( name, value ) { + return access( this, jQuery.attr, name, value, arguments.length > 1 ); + }, + + removeAttr: function( name ) { + return this.each( function() { + jQuery.removeAttr( this, name ); + } ); + } +} ); + +jQuery.extend( { + attr: function( elem, name, value ) { + var ret, hooks, + nType = elem.nodeType; + + // Don't get/set attributes on text, comment and attribute nodes + if ( nType === 3 || nType === 8 || nType === 2 ) { + return; + } + + // Fallback to prop when attributes are not supported + if ( typeof elem.getAttribute === "undefined" ) { + return jQuery.prop( elem, name, value ); + } + + // Attribute hooks are determined by the lowercase version + // Grab necessary hook if one is defined + if ( nType !== 1 || !jQuery.isXMLDoc( elem ) ) { + hooks = jQuery.attrHooks[ name.toLowerCase() ] || + ( jQuery.expr.match.bool.test( name ) ? boolHook : undefined ); + } + + if ( value !== undefined ) { + if ( value === null ) { + jQuery.removeAttr( elem, name ); + return; + } + + if ( hooks && "set" in hooks && + ( ret = hooks.set( elem, value, name ) ) !== undefined ) { + return ret; + } + + elem.setAttribute( name, value + "" ); + return value; + } + + if ( hooks && "get" in hooks && ( ret = hooks.get( elem, name ) ) !== null ) { + return ret; + } + + ret = jQuery.find.attr( elem, name ); + + // Non-existent attributes return null, we normalize to undefined + return ret == null ? undefined : ret; + }, + + attrHooks: { + type: { + set: function( elem, value ) { + if ( !support.radioValue && value === "radio" && + nodeName( elem, "input" ) ) { + var val = elem.value; + elem.setAttribute( "type", value ); + if ( val ) { + elem.value = val; + } + return value; + } + } + } + }, + + removeAttr: function( elem, value ) { + var name, + i = 0, + + // Attribute names can contain non-HTML whitespace characters + // https://html.spec.whatwg.org/multipage/syntax.html#attributes-2 + attrNames = value && value.match( rnothtmlwhite ); + + if ( attrNames && elem.nodeType === 1 ) { + while ( ( name = attrNames[ i++ ] ) ) { + elem.removeAttribute( name ); + } + } + } +} ); + +// Hooks for boolean attributes +boolHook = { + set: function( elem, value, name ) { + if ( value === false ) { + + // Remove boolean attributes when set to false + jQuery.removeAttr( elem, name ); + } else { + elem.setAttribute( name, name ); + } + return name; + } +}; + +jQuery.each( jQuery.expr.match.bool.source.match( /\w+/g ), function( _i, name ) { + var getter = attrHandle[ name ] || jQuery.find.attr; + + attrHandle[ name ] = function( elem, name, isXML ) { + var ret, handle, + lowercaseName = name.toLowerCase(); + + if ( !isXML ) { + + // Avoid an infinite loop by temporarily removing this function from the getter + handle = attrHandle[ lowercaseName ]; + attrHandle[ lowercaseName ] = ret; + ret = getter( elem, name, isXML ) != null ? + lowercaseName : + null; + attrHandle[ lowercaseName ] = handle; + } + return ret; + }; +} ); + + + + +var rfocusable = /^(?:input|select|textarea|button)$/i, + rclickable = /^(?:a|area)$/i; + +jQuery.fn.extend( { + prop: function( name, value ) { + return access( this, jQuery.prop, name, value, arguments.length > 1 ); + }, + + removeProp: function( name ) { + return this.each( function() { + delete this[ jQuery.propFix[ name ] || name ]; + } ); + } +} ); + +jQuery.extend( { + prop: function( elem, name, value ) { + var ret, hooks, + nType = elem.nodeType; + + // Don't get/set properties on text, comment and attribute nodes + if ( nType === 3 || nType === 8 || nType === 2 ) { + return; + } + + if ( nType !== 1 || !jQuery.isXMLDoc( elem ) ) { + + // Fix name and attach hooks + name = jQuery.propFix[ name ] || name; + hooks = jQuery.propHooks[ name ]; + } + + if ( value !== undefined ) { + if ( hooks && "set" in hooks && + ( ret = hooks.set( elem, value, name ) ) !== undefined ) { + return ret; + } + + return ( elem[ name ] = value ); + } + + if ( hooks && "get" in hooks && ( ret = hooks.get( elem, name ) ) !== null ) { + return ret; + } + + return elem[ name ]; + }, + + propHooks: { + tabIndex: { + get: function( elem ) { + + // Support: IE <=9 - 11 only + // elem.tabIndex doesn't always return the + // correct value when it hasn't been explicitly set + // https://web.archive.org/web/20141116233347/http://fluidproject.org/blog/2008/01/09/getting-setting-and-removing-tabindex-values-with-javascript/ + // Use proper attribute retrieval(#12072) + var tabindex = jQuery.find.attr( elem, "tabindex" ); + + if ( tabindex ) { + return parseInt( tabindex, 10 ); + } + + if ( + rfocusable.test( elem.nodeName ) || + rclickable.test( elem.nodeName ) && + elem.href + ) { + return 0; + } + + return -1; + } + } + }, + + propFix: { + "for": "htmlFor", + "class": "className" + } +} ); + +// Support: IE <=11 only +// Accessing the selectedIndex property +// forces the browser to respect setting selected +// on the option +// The getter ensures a default option is selected +// when in an optgroup +// eslint rule "no-unused-expressions" is disabled for this code +// since it considers such accessions noop +if ( !support.optSelected ) { + jQuery.propHooks.selected = { + get: function( elem ) { + + /* eslint no-unused-expressions: "off" */ + + var parent = elem.parentNode; + if ( parent && parent.parentNode ) { + parent.parentNode.selectedIndex; + } + return null; + }, + set: function( elem ) { + + /* eslint no-unused-expressions: "off" */ + + var parent = elem.parentNode; + if ( parent ) { + parent.selectedIndex; + + if ( parent.parentNode ) { + parent.parentNode.selectedIndex; + } + } + } + }; +} + +jQuery.each( [ + "tabIndex", + "readOnly", + "maxLength", + "cellSpacing", + "cellPadding", + "rowSpan", + "colSpan", + "useMap", + "frameBorder", + "contentEditable" +], function() { + jQuery.propFix[ this.toLowerCase() ] = this; +} ); + + + + + // Strip and collapse whitespace according to HTML spec + // https://infra.spec.whatwg.org/#strip-and-collapse-ascii-whitespace + function stripAndCollapse( value ) { + var tokens = value.match( rnothtmlwhite ) || []; + return tokens.join( " " ); + } + + +function getClass( elem ) { + return elem.getAttribute && elem.getAttribute( "class" ) || ""; +} + +function classesToArray( value ) { + if ( Array.isArray( value ) ) { + return value; + } + if ( typeof value === "string" ) { + return value.match( rnothtmlwhite ) || []; + } + return []; +} + +jQuery.fn.extend( { + addClass: function( value ) { + var classes, elem, cur, curValue, clazz, j, finalValue, + i = 0; + + if ( isFunction( value ) ) { + return this.each( function( j ) { + jQuery( this ).addClass( value.call( this, j, getClass( this ) ) ); + } ); + } + + classes = classesToArray( value ); + + if ( classes.length ) { + while ( ( elem = this[ i++ ] ) ) { + curValue = getClass( elem ); + cur = elem.nodeType === 1 && ( " " + stripAndCollapse( curValue ) + " " ); + + if ( cur ) { + j = 0; + while ( ( clazz = classes[ j++ ] ) ) { + if ( cur.indexOf( " " + clazz + " " ) < 0 ) { + cur += clazz + " "; + } + } + + // Only assign if different to avoid unneeded rendering. + finalValue = stripAndCollapse( cur ); + if ( curValue !== finalValue ) { + elem.setAttribute( "class", finalValue ); + } + } + } + } + + return this; + }, + + removeClass: function( value ) { + var classes, elem, cur, curValue, clazz, j, finalValue, + i = 0; + + if ( isFunction( value ) ) { + return this.each( function( j ) { + jQuery( this ).removeClass( value.call( this, j, getClass( this ) ) ); + } ); + } + + if ( !arguments.length ) { + return this.attr( "class", "" ); + } + + classes = classesToArray( value ); + + if ( classes.length ) { + while ( ( elem = this[ i++ ] ) ) { + curValue = getClass( elem ); + + // This expression is here for better compressibility (see addClass) + cur = elem.nodeType === 1 && ( " " + stripAndCollapse( curValue ) + " " ); + + if ( cur ) { + j = 0; + while ( ( clazz = classes[ j++ ] ) ) { + + // Remove *all* instances + while ( cur.indexOf( " " + clazz + " " ) > -1 ) { + cur = cur.replace( " " + clazz + " ", " " ); + } + } + + // Only assign if different to avoid unneeded rendering. + finalValue = stripAndCollapse( cur ); + if ( curValue !== finalValue ) { + elem.setAttribute( "class", finalValue ); + } + } + } + } + + return this; + }, + + toggleClass: function( value, stateVal ) { + var type = typeof value, + isValidValue = type === "string" || Array.isArray( value ); + + if ( typeof stateVal === "boolean" && isValidValue ) { + return stateVal ? this.addClass( value ) : this.removeClass( value ); + } + + if ( isFunction( value ) ) { + return this.each( function( i ) { + jQuery( this ).toggleClass( + value.call( this, i, getClass( this ), stateVal ), + stateVal + ); + } ); + } + + return this.each( function() { + var className, i, self, classNames; + + if ( isValidValue ) { + + // Toggle individual class names + i = 0; + self = jQuery( this ); + classNames = classesToArray( value ); + + while ( ( className = classNames[ i++ ] ) ) { + + // Check each className given, space separated list + if ( self.hasClass( className ) ) { + self.removeClass( className ); + } else { + self.addClass( className ); + } + } + + // Toggle whole class name + } else if ( value === undefined || type === "boolean" ) { + className = getClass( this ); + if ( className ) { + + // Store className if set + dataPriv.set( this, "__className__", className ); + } + + // If the element has a class name or if we're passed `false`, + // then remove the whole classname (if there was one, the above saved it). + // Otherwise bring back whatever was previously saved (if anything), + // falling back to the empty string if nothing was stored. + if ( this.setAttribute ) { + this.setAttribute( "class", + className || value === false ? + "" : + dataPriv.get( this, "__className__" ) || "" + ); + } + } + } ); + }, + + hasClass: function( selector ) { + var className, elem, + i = 0; + + className = " " + selector + " "; + while ( ( elem = this[ i++ ] ) ) { + if ( elem.nodeType === 1 && + ( " " + stripAndCollapse( getClass( elem ) ) + " " ).indexOf( className ) > -1 ) { + return true; + } + } + + return false; + } +} ); + + + + +var rreturn = /\r/g; + +jQuery.fn.extend( { + val: function( value ) { + var hooks, ret, valueIsFunction, + elem = this[ 0 ]; + + if ( !arguments.length ) { + if ( elem ) { + hooks = jQuery.valHooks[ elem.type ] || + jQuery.valHooks[ elem.nodeName.toLowerCase() ]; + + if ( hooks && + "get" in hooks && + ( ret = hooks.get( elem, "value" ) ) !== undefined + ) { + return ret; + } + + ret = elem.value; + + // Handle most common string cases + if ( typeof ret === "string" ) { + return ret.replace( rreturn, "" ); + } + + // Handle cases where value is null/undef or number + return ret == null ? "" : ret; + } + + return; + } + + valueIsFunction = isFunction( value ); + + return this.each( function( i ) { + var val; + + if ( this.nodeType !== 1 ) { + return; + } + + if ( valueIsFunction ) { + val = value.call( this, i, jQuery( this ).val() ); + } else { + val = value; + } + + // Treat null/undefined as ""; convert numbers to string + if ( val == null ) { + val = ""; + + } else if ( typeof val === "number" ) { + val += ""; + + } else if ( Array.isArray( val ) ) { + val = jQuery.map( val, function( value ) { + return value == null ? "" : value + ""; + } ); + } + + hooks = jQuery.valHooks[ this.type ] || jQuery.valHooks[ this.nodeName.toLowerCase() ]; + + // If set returns undefined, fall back to normal setting + if ( !hooks || !( "set" in hooks ) || hooks.set( this, val, "value" ) === undefined ) { + this.value = val; + } + } ); + } +} ); + +jQuery.extend( { + valHooks: { + option: { + get: function( elem ) { + + var val = jQuery.find.attr( elem, "value" ); + return val != null ? + val : + + // Support: IE <=10 - 11 only + // option.text throws exceptions (#14686, #14858) + // Strip and collapse whitespace + // https://html.spec.whatwg.org/#strip-and-collapse-whitespace + stripAndCollapse( jQuery.text( elem ) ); + } + }, + select: { + get: function( elem ) { + var value, option, i, + options = elem.options, + index = elem.selectedIndex, + one = elem.type === "select-one", + values = one ? null : [], + max = one ? index + 1 : options.length; + + if ( index < 0 ) { + i = max; + + } else { + i = one ? index : 0; + } + + // Loop through all the selected options + for ( ; i < max; i++ ) { + option = options[ i ]; + + // Support: IE <=9 only + // IE8-9 doesn't update selected after form reset (#2551) + if ( ( option.selected || i === index ) && + + // Don't return options that are disabled or in a disabled optgroup + !option.disabled && + ( !option.parentNode.disabled || + !nodeName( option.parentNode, "optgroup" ) ) ) { + + // Get the specific value for the option + value = jQuery( option ).val(); + + // We don't need an array for one selects + if ( one ) { + return value; + } + + // Multi-Selects return an array + values.push( value ); + } + } + + return values; + }, + + set: function( elem, value ) { + var optionSet, option, + options = elem.options, + values = jQuery.makeArray( value ), + i = options.length; + + while ( i-- ) { + option = options[ i ]; + + /* eslint-disable no-cond-assign */ + + if ( option.selected = + jQuery.inArray( jQuery.valHooks.option.get( option ), values ) > -1 + ) { + optionSet = true; + } + + /* eslint-enable no-cond-assign */ + } + + // Force browsers to behave consistently when non-matching value is set + if ( !optionSet ) { + elem.selectedIndex = -1; + } + return values; + } + } + } +} ); + +// Radios and checkboxes getter/setter +jQuery.each( [ "radio", "checkbox" ], function() { + jQuery.valHooks[ this ] = { + set: function( elem, value ) { + if ( Array.isArray( value ) ) { + return ( elem.checked = jQuery.inArray( jQuery( elem ).val(), value ) > -1 ); + } + } + }; + if ( !support.checkOn ) { + jQuery.valHooks[ this ].get = function( elem ) { + return elem.getAttribute( "value" ) === null ? "on" : elem.value; + }; + } +} ); + + + + +// Return jQuery for attributes-only inclusion + + +support.focusin = "onfocusin" in window; + + +var rfocusMorph = /^(?:focusinfocus|focusoutblur)$/, + stopPropagationCallback = function( e ) { + e.stopPropagation(); + }; + +jQuery.extend( jQuery.event, { + + trigger: function( event, data, elem, onlyHandlers ) { + + var i, cur, tmp, bubbleType, ontype, handle, special, lastElement, + eventPath = [ elem || document ], + type = hasOwn.call( event, "type" ) ? event.type : event, + namespaces = hasOwn.call( event, "namespace" ) ? event.namespace.split( "." ) : []; + + cur = lastElement = tmp = elem = elem || document; + + // Don't do events on text and comment nodes + if ( elem.nodeType === 3 || elem.nodeType === 8 ) { + return; + } + + // focus/blur morphs to focusin/out; ensure we're not firing them right now + if ( rfocusMorph.test( type + jQuery.event.triggered ) ) { + return; + } + + if ( type.indexOf( "." ) > -1 ) { + + // Namespaced trigger; create a regexp to match event type in handle() + namespaces = type.split( "." ); + type = namespaces.shift(); + namespaces.sort(); + } + ontype = type.indexOf( ":" ) < 0 && "on" + type; + + // Caller can pass in a jQuery.Event object, Object, or just an event type string + event = event[ jQuery.expando ] ? + event : + new jQuery.Event( type, typeof event === "object" && event ); + + // Trigger bitmask: & 1 for native handlers; & 2 for jQuery (always true) + event.isTrigger = onlyHandlers ? 2 : 3; + event.namespace = namespaces.join( "." ); + event.rnamespace = event.namespace ? + new RegExp( "(^|\\.)" + namespaces.join( "\\.(?:.*\\.|)" ) + "(\\.|$)" ) : + null; + + // Clean up the event in case it is being reused + event.result = undefined; + if ( !event.target ) { + event.target = elem; + } + + // Clone any incoming data and prepend the event, creating the handler arg list + data = data == null ? + [ event ] : + jQuery.makeArray( data, [ event ] ); + + // Allow special events to draw outside the lines + special = jQuery.event.special[ type ] || {}; + if ( !onlyHandlers && special.trigger && special.trigger.apply( elem, data ) === false ) { + return; + } + + // Determine event propagation path in advance, per W3C events spec (#9951) + // Bubble up to document, then to window; watch for a global ownerDocument var (#9724) + if ( !onlyHandlers && !special.noBubble && !isWindow( elem ) ) { + + bubbleType = special.delegateType || type; + if ( !rfocusMorph.test( bubbleType + type ) ) { + cur = cur.parentNode; + } + for ( ; cur; cur = cur.parentNode ) { + eventPath.push( cur ); + tmp = cur; + } + + // Only add window if we got to document (e.g., not plain obj or detached DOM) + if ( tmp === ( elem.ownerDocument || document ) ) { + eventPath.push( tmp.defaultView || tmp.parentWindow || window ); + } + } + + // Fire handlers on the event path + i = 0; + while ( ( cur = eventPath[ i++ ] ) && !event.isPropagationStopped() ) { + lastElement = cur; + event.type = i > 1 ? + bubbleType : + special.bindType || type; + + // jQuery handler + handle = ( dataPriv.get( cur, "events" ) || Object.create( null ) )[ event.type ] && + dataPriv.get( cur, "handle" ); + if ( handle ) { + handle.apply( cur, data ); + } + + // Native handler + handle = ontype && cur[ ontype ]; + if ( handle && handle.apply && acceptData( cur ) ) { + event.result = handle.apply( cur, data ); + if ( event.result === false ) { + event.preventDefault(); + } + } + } + event.type = type; + + // If nobody prevented the default action, do it now + if ( !onlyHandlers && !event.isDefaultPrevented() ) { + + if ( ( !special._default || + special._default.apply( eventPath.pop(), data ) === false ) && + acceptData( elem ) ) { + + // Call a native DOM method on the target with the same name as the event. + // Don't do default actions on window, that's where global variables be (#6170) + if ( ontype && isFunction( elem[ type ] ) && !isWindow( elem ) ) { + + // Don't re-trigger an onFOO event when we call its FOO() method + tmp = elem[ ontype ]; + + if ( tmp ) { + elem[ ontype ] = null; + } + + // Prevent re-triggering of the same event, since we already bubbled it above + jQuery.event.triggered = type; + + if ( event.isPropagationStopped() ) { + lastElement.addEventListener( type, stopPropagationCallback ); + } + + elem[ type ](); + + if ( event.isPropagationStopped() ) { + lastElement.removeEventListener( type, stopPropagationCallback ); + } + + jQuery.event.triggered = undefined; + + if ( tmp ) { + elem[ ontype ] = tmp; + } + } + } + } + + return event.result; + }, + + // Piggyback on a donor event to simulate a different one + // Used only for `focus(in | out)` events + simulate: function( type, elem, event ) { + var e = jQuery.extend( + new jQuery.Event(), + event, + { + type: type, + isSimulated: true + } + ); + + jQuery.event.trigger( e, null, elem ); + } + +} ); + +jQuery.fn.extend( { + + trigger: function( type, data ) { + return this.each( function() { + jQuery.event.trigger( type, data, this ); + } ); + }, + triggerHandler: function( type, data ) { + var elem = this[ 0 ]; + if ( elem ) { + return jQuery.event.trigger( type, data, elem, true ); + } + } +} ); + + +// Support: Firefox <=44 +// Firefox doesn't have focus(in | out) events +// Related ticket - https://bugzilla.mozilla.org/show_bug.cgi?id=687787 +// +// Support: Chrome <=48 - 49, Safari <=9.0 - 9.1 +// focus(in | out) events fire after focus & blur events, +// which is spec violation - http://www.w3.org/TR/DOM-Level-3-Events/#events-focusevent-event-order +// Related ticket - https://bugs.chromium.org/p/chromium/issues/detail?id=449857 +if ( !support.focusin ) { + jQuery.each( { focus: "focusin", blur: "focusout" }, function( orig, fix ) { + + // Attach a single capturing handler on the document while someone wants focusin/focusout + var handler = function( event ) { + jQuery.event.simulate( fix, event.target, jQuery.event.fix( event ) ); + }; + + jQuery.event.special[ fix ] = { + setup: function() { + + // Handle: regular nodes (via `this.ownerDocument`), window + // (via `this.document`) & document (via `this`). + var doc = this.ownerDocument || this.document || this, + attaches = dataPriv.access( doc, fix ); + + if ( !attaches ) { + doc.addEventListener( orig, handler, true ); + } + dataPriv.access( doc, fix, ( attaches || 0 ) + 1 ); + }, + teardown: function() { + var doc = this.ownerDocument || this.document || this, + attaches = dataPriv.access( doc, fix ) - 1; + + if ( !attaches ) { + doc.removeEventListener( orig, handler, true ); + dataPriv.remove( doc, fix ); + + } else { + dataPriv.access( doc, fix, attaches ); + } + } + }; + } ); +} +var location = window.location; + +var nonce = { guid: Date.now() }; + +var rquery = ( /\?/ ); + + + +// Cross-browser xml parsing +jQuery.parseXML = function( data ) { + var xml, parserErrorElem; + if ( !data || typeof data !== "string" ) { + return null; + } + + // Support: IE 9 - 11 only + // IE throws on parseFromString with invalid input. + try { + xml = ( new window.DOMParser() ).parseFromString( data, "text/xml" ); + } catch ( e ) {} + + parserErrorElem = xml && xml.getElementsByTagName( "parsererror" )[ 0 ]; + if ( !xml || parserErrorElem ) { + jQuery.error( "Invalid XML: " + ( + parserErrorElem ? + jQuery.map( parserErrorElem.childNodes, function( el ) { + return el.textContent; + } ).join( "\n" ) : + data + ) ); + } + return xml; +}; + + +var + rbracket = /\[\]$/, + rCRLF = /\r?\n/g, + rsubmitterTypes = /^(?:submit|button|image|reset|file)$/i, + rsubmittable = /^(?:input|select|textarea|keygen)/i; + +function buildParams( prefix, obj, traditional, add ) { + var name; + + if ( Array.isArray( obj ) ) { + + // Serialize array item. + jQuery.each( obj, function( i, v ) { + if ( traditional || rbracket.test( prefix ) ) { + + // Treat each array item as a scalar. + add( prefix, v ); + + } else { + + // Item is non-scalar (array or object), encode its numeric index. + buildParams( + prefix + "[" + ( typeof v === "object" && v != null ? i : "" ) + "]", + v, + traditional, + add + ); + } + } ); + + } else if ( !traditional && toType( obj ) === "object" ) { + + // Serialize object item. + for ( name in obj ) { + buildParams( prefix + "[" + name + "]", obj[ name ], traditional, add ); + } + + } else { + + // Serialize scalar item. + add( prefix, obj ); + } +} + +// Serialize an array of form elements or a set of +// key/values into a query string +jQuery.param = function( a, traditional ) { + var prefix, + s = [], + add = function( key, valueOrFunction ) { + + // If value is a function, invoke it and use its return value + var value = isFunction( valueOrFunction ) ? + valueOrFunction() : + valueOrFunction; + + s[ s.length ] = encodeURIComponent( key ) + "=" + + encodeURIComponent( value == null ? "" : value ); + }; + + if ( a == null ) { + return ""; + } + + // If an array was passed in, assume that it is an array of form elements. + if ( Array.isArray( a ) || ( a.jquery && !jQuery.isPlainObject( a ) ) ) { + + // Serialize the form elements + jQuery.each( a, function() { + add( this.name, this.value ); + } ); + + } else { + + // If traditional, encode the "old" way (the way 1.3.2 or older + // did it), otherwise encode params recursively. + for ( prefix in a ) { + buildParams( prefix, a[ prefix ], traditional, add ); + } + } + + // Return the resulting serialization + return s.join( "&" ); +}; + +jQuery.fn.extend( { + serialize: function() { + return jQuery.param( this.serializeArray() ); + }, + serializeArray: function() { + return this.map( function() { + + // Can add propHook for "elements" to filter or add form elements + var elements = jQuery.prop( this, "elements" ); + return elements ? jQuery.makeArray( elements ) : this; + } ).filter( function() { + var type = this.type; + + // Use .is( ":disabled" ) so that fieldset[disabled] works + return this.name && !jQuery( this ).is( ":disabled" ) && + rsubmittable.test( this.nodeName ) && !rsubmitterTypes.test( type ) && + ( this.checked || !rcheckableType.test( type ) ); + } ).map( function( _i, elem ) { + var val = jQuery( this ).val(); + + if ( val == null ) { + return null; + } + + if ( Array.isArray( val ) ) { + return jQuery.map( val, function( val ) { + return { name: elem.name, value: val.replace( rCRLF, "\r\n" ) }; + } ); + } + + return { name: elem.name, value: val.replace( rCRLF, "\r\n" ) }; + } ).get(); + } +} ); + + +var + r20 = /%20/g, + rhash = /#.*$/, + rantiCache = /([?&])_=[^&]*/, + rheaders = /^(.*?):[ \t]*([^\r\n]*)$/mg, + + // #7653, #8125, #8152: local protocol detection + rlocalProtocol = /^(?:about|app|app-storage|.+-extension|file|res|widget):$/, + rnoContent = /^(?:GET|HEAD)$/, + rprotocol = /^\/\//, + + /* Prefilters + * 1) They are useful to introduce custom dataTypes (see ajax/jsonp.js for an example) + * 2) These are called: + * - BEFORE asking for a transport + * - AFTER param serialization (s.data is a string if s.processData is true) + * 3) key is the dataType + * 4) the catchall symbol "*" can be used + * 5) execution will start with transport dataType and THEN continue down to "*" if needed + */ + prefilters = {}, + + /* Transports bindings + * 1) key is the dataType + * 2) the catchall symbol "*" can be used + * 3) selection will start with transport dataType and THEN go to "*" if needed + */ + transports = {}, + + // Avoid comment-prolog char sequence (#10098); must appease lint and evade compression + allTypes = "*/".concat( "*" ), + + // Anchor tag for parsing the document origin + originAnchor = document.createElement( "a" ); + +originAnchor.href = location.href; + +// Base "constructor" for jQuery.ajaxPrefilter and jQuery.ajaxTransport +function addToPrefiltersOrTransports( structure ) { + + // dataTypeExpression is optional and defaults to "*" + return function( dataTypeExpression, func ) { + + if ( typeof dataTypeExpression !== "string" ) { + func = dataTypeExpression; + dataTypeExpression = "*"; + } + + var dataType, + i = 0, + dataTypes = dataTypeExpression.toLowerCase().match( rnothtmlwhite ) || []; + + if ( isFunction( func ) ) { + + // For each dataType in the dataTypeExpression + while ( ( dataType = dataTypes[ i++ ] ) ) { + + // Prepend if requested + if ( dataType[ 0 ] === "+" ) { + dataType = dataType.slice( 1 ) || "*"; + ( structure[ dataType ] = structure[ dataType ] || [] ).unshift( func ); + + // Otherwise append + } else { + ( structure[ dataType ] = structure[ dataType ] || [] ).push( func ); + } + } + } + }; +} + +// Base inspection function for prefilters and transports +function inspectPrefiltersOrTransports( structure, options, originalOptions, jqXHR ) { + + var inspected = {}, + seekingTransport = ( structure === transports ); + + function inspect( dataType ) { + var selected; + inspected[ dataType ] = true; + jQuery.each( structure[ dataType ] || [], function( _, prefilterOrFactory ) { + var dataTypeOrTransport = prefilterOrFactory( options, originalOptions, jqXHR ); + if ( typeof dataTypeOrTransport === "string" && + !seekingTransport && !inspected[ dataTypeOrTransport ] ) { + + options.dataTypes.unshift( dataTypeOrTransport ); + inspect( dataTypeOrTransport ); + return false; + } else if ( seekingTransport ) { + return !( selected = dataTypeOrTransport ); + } + } ); + return selected; + } + + return inspect( options.dataTypes[ 0 ] ) || !inspected[ "*" ] && inspect( "*" ); +} + +// A special extend for ajax options +// that takes "flat" options (not to be deep extended) +// Fixes #9887 +function ajaxExtend( target, src ) { + var key, deep, + flatOptions = jQuery.ajaxSettings.flatOptions || {}; + + for ( key in src ) { + if ( src[ key ] !== undefined ) { + ( flatOptions[ key ] ? target : ( deep || ( deep = {} ) ) )[ key ] = src[ key ]; + } + } + if ( deep ) { + jQuery.extend( true, target, deep ); + } + + return target; +} + +/* Handles responses to an ajax request: + * - finds the right dataType (mediates between content-type and expected dataType) + * - returns the corresponding response + */ +function ajaxHandleResponses( s, jqXHR, responses ) { + + var ct, type, finalDataType, firstDataType, + contents = s.contents, + dataTypes = s.dataTypes; + + // Remove auto dataType and get content-type in the process + while ( dataTypes[ 0 ] === "*" ) { + dataTypes.shift(); + if ( ct === undefined ) { + ct = s.mimeType || jqXHR.getResponseHeader( "Content-Type" ); + } + } + + // Check if we're dealing with a known content-type + if ( ct ) { + for ( type in contents ) { + if ( contents[ type ] && contents[ type ].test( ct ) ) { + dataTypes.unshift( type ); + break; + } + } + } + + // Check to see if we have a response for the expected dataType + if ( dataTypes[ 0 ] in responses ) { + finalDataType = dataTypes[ 0 ]; + } else { + + // Try convertible dataTypes + for ( type in responses ) { + if ( !dataTypes[ 0 ] || s.converters[ type + " " + dataTypes[ 0 ] ] ) { + finalDataType = type; + break; + } + if ( !firstDataType ) { + firstDataType = type; + } + } + + // Or just use first one + finalDataType = finalDataType || firstDataType; + } + + // If we found a dataType + // We add the dataType to the list if needed + // and return the corresponding response + if ( finalDataType ) { + if ( finalDataType !== dataTypes[ 0 ] ) { + dataTypes.unshift( finalDataType ); + } + return responses[ finalDataType ]; + } +} + +/* Chain conversions given the request and the original response + * Also sets the responseXXX fields on the jqXHR instance + */ +function ajaxConvert( s, response, jqXHR, isSuccess ) { + var conv2, current, conv, tmp, prev, + converters = {}, + + // Work with a copy of dataTypes in case we need to modify it for conversion + dataTypes = s.dataTypes.slice(); + + // Create converters map with lowercased keys + if ( dataTypes[ 1 ] ) { + for ( conv in s.converters ) { + converters[ conv.toLowerCase() ] = s.converters[ conv ]; + } + } + + current = dataTypes.shift(); + + // Convert to each sequential dataType + while ( current ) { + + if ( s.responseFields[ current ] ) { + jqXHR[ s.responseFields[ current ] ] = response; + } + + // Apply the dataFilter if provided + if ( !prev && isSuccess && s.dataFilter ) { + response = s.dataFilter( response, s.dataType ); + } + + prev = current; + current = dataTypes.shift(); + + if ( current ) { + + // There's only work to do if current dataType is non-auto + if ( current === "*" ) { + + current = prev; + + // Convert response if prev dataType is non-auto and differs from current + } else if ( prev !== "*" && prev !== current ) { + + // Seek a direct converter + conv = converters[ prev + " " + current ] || converters[ "* " + current ]; + + // If none found, seek a pair + if ( !conv ) { + for ( conv2 in converters ) { + + // If conv2 outputs current + tmp = conv2.split( " " ); + if ( tmp[ 1 ] === current ) { + + // If prev can be converted to accepted input + conv = converters[ prev + " " + tmp[ 0 ] ] || + converters[ "* " + tmp[ 0 ] ]; + if ( conv ) { + + // Condense equivalence converters + if ( conv === true ) { + conv = converters[ conv2 ]; + + // Otherwise, insert the intermediate dataType + } else if ( converters[ conv2 ] !== true ) { + current = tmp[ 0 ]; + dataTypes.unshift( tmp[ 1 ] ); + } + break; + } + } + } + } + + // Apply converter (if not an equivalence) + if ( conv !== true ) { + + // Unless errors are allowed to bubble, catch and return them + if ( conv && s.throws ) { + response = conv( response ); + } else { + try { + response = conv( response ); + } catch ( e ) { + return { + state: "parsererror", + error: conv ? e : "No conversion from " + prev + " to " + current + }; + } + } + } + } + } + } + + return { state: "success", data: response }; +} + +jQuery.extend( { + + // Counter for holding the number of active queries + active: 0, + + // Last-Modified header cache for next request + lastModified: {}, + etag: {}, + + ajaxSettings: { + url: location.href, + type: "GET", + isLocal: rlocalProtocol.test( location.protocol ), + global: true, + processData: true, + async: true, + contentType: "application/x-www-form-urlencoded; charset=UTF-8", + + /* + timeout: 0, + data: null, + dataType: null, + username: null, + password: null, + cache: null, + throws: false, + traditional: false, + headers: {}, + */ + + accepts: { + "*": allTypes, + text: "text/plain", + html: "text/html", + xml: "application/xml, text/xml", + json: "application/json, text/javascript" + }, + + contents: { + xml: /\bxml\b/, + html: /\bhtml/, + json: /\bjson\b/ + }, + + responseFields: { + xml: "responseXML", + text: "responseText", + json: "responseJSON" + }, + + // Data converters + // Keys separate source (or catchall "*") and destination types with a single space + converters: { + + // Convert anything to text + "* text": String, + + // Text to html (true = no transformation) + "text html": true, + + // Evaluate text as a json expression + "text json": JSON.parse, + + // Parse text as xml + "text xml": jQuery.parseXML + }, + + // For options that shouldn't be deep extended: + // you can add your own custom options here if + // and when you create one that shouldn't be + // deep extended (see ajaxExtend) + flatOptions: { + url: true, + context: true + } + }, + + // Creates a full fledged settings object into target + // with both ajaxSettings and settings fields. + // If target is omitted, writes into ajaxSettings. + ajaxSetup: function( target, settings ) { + return settings ? + + // Building a settings object + ajaxExtend( ajaxExtend( target, jQuery.ajaxSettings ), settings ) : + + // Extending ajaxSettings + ajaxExtend( jQuery.ajaxSettings, target ); + }, + + ajaxPrefilter: addToPrefiltersOrTransports( prefilters ), + ajaxTransport: addToPrefiltersOrTransports( transports ), + + // Main method + ajax: function( url, options ) { + + // If url is an object, simulate pre-1.5 signature + if ( typeof url === "object" ) { + options = url; + url = undefined; + } + + // Force options to be an object + options = options || {}; + + var transport, + + // URL without anti-cache param + cacheURL, + + // Response headers + responseHeadersString, + responseHeaders, + + // timeout handle + timeoutTimer, + + // Url cleanup var + urlAnchor, + + // Request state (becomes false upon send and true upon completion) + completed, + + // To know if global events are to be dispatched + fireGlobals, + + // Loop variable + i, + + // uncached part of the url + uncached, + + // Create the final options object + s = jQuery.ajaxSetup( {}, options ), + + // Callbacks context + callbackContext = s.context || s, + + // Context for global events is callbackContext if it is a DOM node or jQuery collection + globalEventContext = s.context && + ( callbackContext.nodeType || callbackContext.jquery ) ? + jQuery( callbackContext ) : + jQuery.event, + + // Deferreds + deferred = jQuery.Deferred(), + completeDeferred = jQuery.Callbacks( "once memory" ), + + // Status-dependent callbacks + statusCode = s.statusCode || {}, + + // Headers (they are sent all at once) + requestHeaders = {}, + requestHeadersNames = {}, + + // Default abort message + strAbort = "canceled", + + // Fake xhr + jqXHR = { + readyState: 0, + + // Builds headers hashtable if needed + getResponseHeader: function( key ) { + var match; + if ( completed ) { + if ( !responseHeaders ) { + responseHeaders = {}; + while ( ( match = rheaders.exec( responseHeadersString ) ) ) { + responseHeaders[ match[ 1 ].toLowerCase() + " " ] = + ( responseHeaders[ match[ 1 ].toLowerCase() + " " ] || [] ) + .concat( match[ 2 ] ); + } + } + match = responseHeaders[ key.toLowerCase() + " " ]; + } + return match == null ? null : match.join( ", " ); + }, + + // Raw string + getAllResponseHeaders: function() { + return completed ? responseHeadersString : null; + }, + + // Caches the header + setRequestHeader: function( name, value ) { + if ( completed == null ) { + name = requestHeadersNames[ name.toLowerCase() ] = + requestHeadersNames[ name.toLowerCase() ] || name; + requestHeaders[ name ] = value; + } + return this; + }, + + // Overrides response content-type header + overrideMimeType: function( type ) { + if ( completed == null ) { + s.mimeType = type; + } + return this; + }, + + // Status-dependent callbacks + statusCode: function( map ) { + var code; + if ( map ) { + if ( completed ) { + + // Execute the appropriate callbacks + jqXHR.always( map[ jqXHR.status ] ); + } else { + + // Lazy-add the new callbacks in a way that preserves old ones + for ( code in map ) { + statusCode[ code ] = [ statusCode[ code ], map[ code ] ]; + } + } + } + return this; + }, + + // Cancel the request + abort: function( statusText ) { + var finalText = statusText || strAbort; + if ( transport ) { + transport.abort( finalText ); + } + done( 0, finalText ); + return this; + } + }; + + // Attach deferreds + deferred.promise( jqXHR ); + + // Add protocol if not provided (prefilters might expect it) + // Handle falsy url in the settings object (#10093: consistency with old signature) + // We also use the url parameter if available + s.url = ( ( url || s.url || location.href ) + "" ) + .replace( rprotocol, location.protocol + "//" ); + + // Alias method option to type as per ticket #12004 + s.type = options.method || options.type || s.method || s.type; + + // Extract dataTypes list + s.dataTypes = ( s.dataType || "*" ).toLowerCase().match( rnothtmlwhite ) || [ "" ]; + + // A cross-domain request is in order when the origin doesn't match the current origin. + if ( s.crossDomain == null ) { + urlAnchor = document.createElement( "a" ); + + // Support: IE <=8 - 11, Edge 12 - 15 + // IE throws exception on accessing the href property if url is malformed, + // e.g. http://example.com:80x/ + try { + urlAnchor.href = s.url; + + // Support: IE <=8 - 11 only + // Anchor's host property isn't correctly set when s.url is relative + urlAnchor.href = urlAnchor.href; + s.crossDomain = originAnchor.protocol + "//" + originAnchor.host !== + urlAnchor.protocol + "//" + urlAnchor.host; + } catch ( e ) { + + // If there is an error parsing the URL, assume it is crossDomain, + // it can be rejected by the transport if it is invalid + s.crossDomain = true; + } + } + + // Convert data if not already a string + if ( s.data && s.processData && typeof s.data !== "string" ) { + s.data = jQuery.param( s.data, s.traditional ); + } + + // Apply prefilters + inspectPrefiltersOrTransports( prefilters, s, options, jqXHR ); + + // If request was aborted inside a prefilter, stop there + if ( completed ) { + return jqXHR; + } + + // We can fire global events as of now if asked to + // Don't fire events if jQuery.event is undefined in an AMD-usage scenario (#15118) + fireGlobals = jQuery.event && s.global; + + // Watch for a new set of requests + if ( fireGlobals && jQuery.active++ === 0 ) { + jQuery.event.trigger( "ajaxStart" ); + } + + // Uppercase the type + s.type = s.type.toUpperCase(); + + // Determine if request has content + s.hasContent = !rnoContent.test( s.type ); + + // Save the URL in case we're toying with the If-Modified-Since + // and/or If-None-Match header later on + // Remove hash to simplify url manipulation + cacheURL = s.url.replace( rhash, "" ); + + // More options handling for requests with no content + if ( !s.hasContent ) { + + // Remember the hash so we can put it back + uncached = s.url.slice( cacheURL.length ); + + // If data is available and should be processed, append data to url + if ( s.data && ( s.processData || typeof s.data === "string" ) ) { + cacheURL += ( rquery.test( cacheURL ) ? "&" : "?" ) + s.data; + + // #9682: remove data so that it's not used in an eventual retry + delete s.data; + } + + // Add or update anti-cache param if needed + if ( s.cache === false ) { + cacheURL = cacheURL.replace( rantiCache, "$1" ); + uncached = ( rquery.test( cacheURL ) ? "&" : "?" ) + "_=" + ( nonce.guid++ ) + + uncached; + } + + // Put hash and anti-cache on the URL that will be requested (gh-1732) + s.url = cacheURL + uncached; + + // Change '%20' to '+' if this is encoded form body content (gh-2658) + } else if ( s.data && s.processData && + ( s.contentType || "" ).indexOf( "application/x-www-form-urlencoded" ) === 0 ) { + s.data = s.data.replace( r20, "+" ); + } + + // Set the If-Modified-Since and/or If-None-Match header, if in ifModified mode. + if ( s.ifModified ) { + if ( jQuery.lastModified[ cacheURL ] ) { + jqXHR.setRequestHeader( "If-Modified-Since", jQuery.lastModified[ cacheURL ] ); + } + if ( jQuery.etag[ cacheURL ] ) { + jqXHR.setRequestHeader( "If-None-Match", jQuery.etag[ cacheURL ] ); + } + } + + // Set the correct header, if data is being sent + if ( s.data && s.hasContent && s.contentType !== false || options.contentType ) { + jqXHR.setRequestHeader( "Content-Type", s.contentType ); + } + + // Set the Accepts header for the server, depending on the dataType + jqXHR.setRequestHeader( + "Accept", + s.dataTypes[ 0 ] && s.accepts[ s.dataTypes[ 0 ] ] ? + s.accepts[ s.dataTypes[ 0 ] ] + + ( s.dataTypes[ 0 ] !== "*" ? ", " + allTypes + "; q=0.01" : "" ) : + s.accepts[ "*" ] + ); + + // Check for headers option + for ( i in s.headers ) { + jqXHR.setRequestHeader( i, s.headers[ i ] ); + } + + // Allow custom headers/mimetypes and early abort + if ( s.beforeSend && + ( s.beforeSend.call( callbackContext, jqXHR, s ) === false || completed ) ) { + + // Abort if not done already and return + return jqXHR.abort(); + } + + // Aborting is no longer a cancellation + strAbort = "abort"; + + // Install callbacks on deferreds + completeDeferred.add( s.complete ); + jqXHR.done( s.success ); + jqXHR.fail( s.error ); + + // Get transport + transport = inspectPrefiltersOrTransports( transports, s, options, jqXHR ); + + // If no transport, we auto-abort + if ( !transport ) { + done( -1, "No Transport" ); + } else { + jqXHR.readyState = 1; + + // Send global event + if ( fireGlobals ) { + globalEventContext.trigger( "ajaxSend", [ jqXHR, s ] ); + } + + // If request was aborted inside ajaxSend, stop there + if ( completed ) { + return jqXHR; + } + + // Timeout + if ( s.async && s.timeout > 0 ) { + timeoutTimer = window.setTimeout( function() { + jqXHR.abort( "timeout" ); + }, s.timeout ); + } + + try { + completed = false; + transport.send( requestHeaders, done ); + } catch ( e ) { + + // Rethrow post-completion exceptions + if ( completed ) { + throw e; + } + + // Propagate others as results + done( -1, e ); + } + } + + // Callback for when everything is done + function done( status, nativeStatusText, responses, headers ) { + var isSuccess, success, error, response, modified, + statusText = nativeStatusText; + + // Ignore repeat invocations + if ( completed ) { + return; + } + + completed = true; + + // Clear timeout if it exists + if ( timeoutTimer ) { + window.clearTimeout( timeoutTimer ); + } + + // Dereference transport for early garbage collection + // (no matter how long the jqXHR object will be used) + transport = undefined; + + // Cache response headers + responseHeadersString = headers || ""; + + // Set readyState + jqXHR.readyState = status > 0 ? 4 : 0; + + // Determine if successful + isSuccess = status >= 200 && status < 300 || status === 304; + + // Get response data + if ( responses ) { + response = ajaxHandleResponses( s, jqXHR, responses ); + } + + // Use a noop converter for missing script but not if jsonp + if ( !isSuccess && + jQuery.inArray( "script", s.dataTypes ) > -1 && + jQuery.inArray( "json", s.dataTypes ) < 0 ) { + s.converters[ "text script" ] = function() {}; + } + + // Convert no matter what (that way responseXXX fields are always set) + response = ajaxConvert( s, response, jqXHR, isSuccess ); + + // If successful, handle type chaining + if ( isSuccess ) { + + // Set the If-Modified-Since and/or If-None-Match header, if in ifModified mode. + if ( s.ifModified ) { + modified = jqXHR.getResponseHeader( "Last-Modified" ); + if ( modified ) { + jQuery.lastModified[ cacheURL ] = modified; + } + modified = jqXHR.getResponseHeader( "etag" ); + if ( modified ) { + jQuery.etag[ cacheURL ] = modified; + } + } + + // if no content + if ( status === 204 || s.type === "HEAD" ) { + statusText = "nocontent"; + + // if not modified + } else if ( status === 304 ) { + statusText = "notmodified"; + + // If we have data, let's convert it + } else { + statusText = response.state; + success = response.data; + error = response.error; + isSuccess = !error; + } + } else { + + // Extract error from statusText and normalize for non-aborts + error = statusText; + if ( status || !statusText ) { + statusText = "error"; + if ( status < 0 ) { + status = 0; + } + } + } + + // Set data for the fake xhr object + jqXHR.status = status; + jqXHR.statusText = ( nativeStatusText || statusText ) + ""; + + // Success/Error + if ( isSuccess ) { + deferred.resolveWith( callbackContext, [ success, statusText, jqXHR ] ); + } else { + deferred.rejectWith( callbackContext, [ jqXHR, statusText, error ] ); + } + + // Status-dependent callbacks + jqXHR.statusCode( statusCode ); + statusCode = undefined; + + if ( fireGlobals ) { + globalEventContext.trigger( isSuccess ? "ajaxSuccess" : "ajaxError", + [ jqXHR, s, isSuccess ? success : error ] ); + } + + // Complete + completeDeferred.fireWith( callbackContext, [ jqXHR, statusText ] ); + + if ( fireGlobals ) { + globalEventContext.trigger( "ajaxComplete", [ jqXHR, s ] ); + + // Handle the global AJAX counter + if ( !( --jQuery.active ) ) { + jQuery.event.trigger( "ajaxStop" ); + } + } + } + + return jqXHR; + }, + + getJSON: function( url, data, callback ) { + return jQuery.get( url, data, callback, "json" ); + }, + + getScript: function( url, callback ) { + return jQuery.get( url, undefined, callback, "script" ); + } +} ); + +jQuery.each( [ "get", "post" ], function( _i, method ) { + jQuery[ method ] = function( url, data, callback, type ) { + + // Shift arguments if data argument was omitted + if ( isFunction( data ) ) { + type = type || callback; + callback = data; + data = undefined; + } + + // The url can be an options object (which then must have .url) + return jQuery.ajax( jQuery.extend( { + url: url, + type: method, + dataType: type, + data: data, + success: callback + }, jQuery.isPlainObject( url ) && url ) ); + }; +} ); + +jQuery.ajaxPrefilter( function( s ) { + var i; + for ( i in s.headers ) { + if ( i.toLowerCase() === "content-type" ) { + s.contentType = s.headers[ i ] || ""; + } + } +} ); + + +jQuery._evalUrl = function( url, options, doc ) { + return jQuery.ajax( { + url: url, + + // Make this explicit, since user can override this through ajaxSetup (#11264) + type: "GET", + dataType: "script", + cache: true, + async: false, + global: false, + + // Only evaluate the response if it is successful (gh-4126) + // dataFilter is not invoked for failure responses, so using it instead + // of the default converter is kludgy but it works. + converters: { + "text script": function() {} + }, + dataFilter: function( response ) { + jQuery.globalEval( response, options, doc ); + } + } ); +}; + + +jQuery.fn.extend( { + wrapAll: function( html ) { + var wrap; + + if ( this[ 0 ] ) { + if ( isFunction( html ) ) { + html = html.call( this[ 0 ] ); + } + + // The elements to wrap the target around + wrap = jQuery( html, this[ 0 ].ownerDocument ).eq( 0 ).clone( true ); + + if ( this[ 0 ].parentNode ) { + wrap.insertBefore( this[ 0 ] ); + } + + wrap.map( function() { + var elem = this; + + while ( elem.firstElementChild ) { + elem = elem.firstElementChild; + } + + return elem; + } ).append( this ); + } + + return this; + }, + + wrapInner: function( html ) { + if ( isFunction( html ) ) { + return this.each( function( i ) { + jQuery( this ).wrapInner( html.call( this, i ) ); + } ); + } + + return this.each( function() { + var self = jQuery( this ), + contents = self.contents(); + + if ( contents.length ) { + contents.wrapAll( html ); + + } else { + self.append( html ); + } + } ); + }, + + wrap: function( html ) { + var htmlIsFunction = isFunction( html ); + + return this.each( function( i ) { + jQuery( this ).wrapAll( htmlIsFunction ? html.call( this, i ) : html ); + } ); + }, + + unwrap: function( selector ) { + this.parent( selector ).not( "body" ).each( function() { + jQuery( this ).replaceWith( this.childNodes ); + } ); + return this; + } +} ); + + +jQuery.expr.pseudos.hidden = function( elem ) { + return !jQuery.expr.pseudos.visible( elem ); +}; +jQuery.expr.pseudos.visible = function( elem ) { + return !!( elem.offsetWidth || elem.offsetHeight || elem.getClientRects().length ); +}; + + + + +jQuery.ajaxSettings.xhr = function() { + try { + return new window.XMLHttpRequest(); + } catch ( e ) {} +}; + +var xhrSuccessStatus = { + + // File protocol always yields status code 0, assume 200 + 0: 200, + + // Support: IE <=9 only + // #1450: sometimes IE returns 1223 when it should be 204 + 1223: 204 + }, + xhrSupported = jQuery.ajaxSettings.xhr(); + +support.cors = !!xhrSupported && ( "withCredentials" in xhrSupported ); +support.ajax = xhrSupported = !!xhrSupported; + +jQuery.ajaxTransport( function( options ) { + var callback, errorCallback; + + // Cross domain only allowed if supported through XMLHttpRequest + if ( support.cors || xhrSupported && !options.crossDomain ) { + return { + send: function( headers, complete ) { + var i, + xhr = options.xhr(); + + xhr.open( + options.type, + options.url, + options.async, + options.username, + options.password + ); + + // Apply custom fields if provided + if ( options.xhrFields ) { + for ( i in options.xhrFields ) { + xhr[ i ] = options.xhrFields[ i ]; + } + } + + // Override mime type if needed + if ( options.mimeType && xhr.overrideMimeType ) { + xhr.overrideMimeType( options.mimeType ); + } + + // X-Requested-With header + // For cross-domain requests, seeing as conditions for a preflight are + // akin to a jigsaw puzzle, we simply never set it to be sure. + // (it can always be set on a per-request basis or even using ajaxSetup) + // For same-domain requests, won't change header if already provided. + if ( !options.crossDomain && !headers[ "X-Requested-With" ] ) { + headers[ "X-Requested-With" ] = "XMLHttpRequest"; + } + + // Set headers + for ( i in headers ) { + xhr.setRequestHeader( i, headers[ i ] ); + } + + // Callback + callback = function( type ) { + return function() { + if ( callback ) { + callback = errorCallback = xhr.onload = + xhr.onerror = xhr.onabort = xhr.ontimeout = + xhr.onreadystatechange = null; + + if ( type === "abort" ) { + xhr.abort(); + } else if ( type === "error" ) { + + // Support: IE <=9 only + // On a manual native abort, IE9 throws + // errors on any property access that is not readyState + if ( typeof xhr.status !== "number" ) { + complete( 0, "error" ); + } else { + complete( + + // File: protocol always yields status 0; see #8605, #14207 + xhr.status, + xhr.statusText + ); + } + } else { + complete( + xhrSuccessStatus[ xhr.status ] || xhr.status, + xhr.statusText, + + // Support: IE <=9 only + // IE9 has no XHR2 but throws on binary (trac-11426) + // For XHR2 non-text, let the caller handle it (gh-2498) + ( xhr.responseType || "text" ) !== "text" || + typeof xhr.responseText !== "string" ? + { binary: xhr.response } : + { text: xhr.responseText }, + xhr.getAllResponseHeaders() + ); + } + } + }; + }; + + // Listen to events + xhr.onload = callback(); + errorCallback = xhr.onerror = xhr.ontimeout = callback( "error" ); + + // Support: IE 9 only + // Use onreadystatechange to replace onabort + // to handle uncaught aborts + if ( xhr.onabort !== undefined ) { + xhr.onabort = errorCallback; + } else { + xhr.onreadystatechange = function() { + + // Check readyState before timeout as it changes + if ( xhr.readyState === 4 ) { + + // Allow onerror to be called first, + // but that will not handle a native abort + // Also, save errorCallback to a variable + // as xhr.onerror cannot be accessed + window.setTimeout( function() { + if ( callback ) { + errorCallback(); + } + } ); + } + }; + } + + // Create the abort callback + callback = callback( "abort" ); + + try { + + // Do send the request (this may raise an exception) + xhr.send( options.hasContent && options.data || null ); + } catch ( e ) { + + // #14683: Only rethrow if this hasn't been notified as an error yet + if ( callback ) { + throw e; + } + } + }, + + abort: function() { + if ( callback ) { + callback(); + } + } + }; + } +} ); + + + + +// Prevent auto-execution of scripts when no explicit dataType was provided (See gh-2432) +jQuery.ajaxPrefilter( function( s ) { + if ( s.crossDomain ) { + s.contents.script = false; + } +} ); + +// Install script dataType +jQuery.ajaxSetup( { + accepts: { + script: "text/javascript, application/javascript, " + + "application/ecmascript, application/x-ecmascript" + }, + contents: { + script: /\b(?:java|ecma)script\b/ + }, + converters: { + "text script": function( text ) { + jQuery.globalEval( text ); + return text; + } + } +} ); + +// Handle cache's special case and crossDomain +jQuery.ajaxPrefilter( "script", function( s ) { + if ( s.cache === undefined ) { + s.cache = false; + } + if ( s.crossDomain ) { + s.type = "GET"; + } +} ); + +// Bind script tag hack transport +jQuery.ajaxTransport( "script", function( s ) { + + // This transport only deals with cross domain or forced-by-attrs requests + if ( s.crossDomain || s.scriptAttrs ) { + var script, callback; + return { + send: function( _, complete ) { + script = jQuery( "