diff --git a/altair/jupyter/js/index.js b/altair/jupyter/js/index.js
index 779514f86..57e8aa800 100644
--- a/altair/jupyter/js/index.js
+++ b/altair/jupyter/js/index.js
@@ -1,6 +1,11 @@
 import vegaEmbed from "https://esm.sh/vega-embed@6?deps=vega@5&deps=vega-lite@5.16.3";
 import lodashDebounce from "https://esm.sh/lodash-es@4.17.21/debounce";
 
+// Note: For offline support, the import lines above are removed and the remaining script
+// is bundled using vl-convert's javascript_bundle function. See the documentation of
+// the javascript_bundle function for details on the available imports and their names.
+// If an additional import is required in the future, it will need to be added to vl-convert
+// in order to preserve offline support.
 export async function render({ model, el }) {
     let finalize;
 
diff --git a/altair/jupyter/jupyter_chart.py b/altair/jupyter/jupyter_chart.py
index cb5b8e0f6..0331c9820 100644
--- a/altair/jupyter/jupyter_chart.py
+++ b/altair/jupyter/jupyter_chart.py
@@ -93,8 +93,12 @@ def _set_value(self, key, value):
         self.observe(self._make_read_only, names=key)
 
 
+def load_js_src() -> str:
+    return (_here / "js" / "index.js").read_text()
+
+
 class JupyterChart(anywidget.AnyWidget):
-    _esm = (_here / "js" / "index.js").read_text()
+    _esm = load_js_src()
     _css = r"""
     .vega-embed {
         /* Make sure action menu isn't cut off */
@@ -124,6 +128,57 @@ class JupyterChart(anywidget.AnyWidget):
     _js_to_py_updates = traitlets.Any(allow_none=True).tag(sync=True)
     _py_to_js_updates = traitlets.Any(allow_none=True).tag(sync=True)
 
+    # Track whether charts are configured for offline use
+    _is_offline = False
+
+    @classmethod
+    def enable_offline(cls, offline: bool = True):
+        """
+        Configure JupyterChart's offline behavior
+
+        Parameters
+        ----------
+        offline: bool
+            If True, configure JupyterChart to operate in offline mode where JavaScript
+            dependencies are loaded from vl-convert.
+            If False, configure it to operate in online mode where JavaScript dependencies
+            are loaded from CDN dynamically. This is the default behavior.
+        """
+        from altair.utils._importers import import_vl_convert, vl_version_for_vl_convert
+
+        if offline:
+            if cls._is_offline:
+                # Already offline
+                return
+
+            vlc = import_vl_convert()
+
+            src_lines = load_js_src().split("\n")
+
+            # Remove leading lines with only whitespace, comments, or imports
+            while src_lines and (
+                len(src_lines[0].strip()) == 0
+                or src_lines[0].startswith("import")
+                or src_lines[0].startswith("//")
+            ):
+                src_lines.pop(0)
+
+            src = "\n".join(src_lines)
+
+            # vl-convert's javascript_bundle function creates a self-contained JavaScript bundle
+            # for JavaScript snippets that import from a small set of dependencies that
+            # vl-convert includes. To see the available imports and their imported names, run
+            #       import vl_convert as vlc
+            #       help(vlc.javascript_bundle)
+            bundled_src = vlc.javascript_bundle(
+                src, vl_version=vl_version_for_vl_convert()
+            )
+            cls._esm = bundled_src
+            cls._is_offline = True
+        else:
+            cls._esm = load_js_src()
+            cls._is_offline = False
+
     def __init__(
         self,
         chart: TopLevelSpec,
diff --git a/altair/vegalite/v5/display.py b/altair/vegalite/v5/display.py
index 0b0a5b66a..e3733adcf 100644
--- a/altair/vegalite/v5/display.py
+++ b/altair/vegalite/v5/display.py
@@ -90,6 +90,13 @@ def jupyter_renderer(spec: dict, **metadata):
     """Render chart using the JupyterChart Jupyter Widget"""
     from altair import Chart, JupyterChart
 
+    # Configure offline mode
+    offline = metadata.get("offline", False)
+
+    # mypy doesn't see the enable_offline class method for some reason
+    JupyterChart.enable_offline(offline=offline)  # type: ignore[attr-defined]
+
+    # propagate embed options
     embed_options = metadata.get("embed_options", None)
 
     # Need to ignore attr-defined mypy rule because mypy doesn't see _repr_mimebundle_
diff --git a/doc/releases/changes.rst b/doc/releases/changes.rst
index 432975514..d065b28bf 100644
--- a/doc/releases/changes.rst
+++ b/doc/releases/changes.rst
@@ -9,6 +9,8 @@ Version 5.3.0 (unreleased month day, year)
 Enhancements
 ~~~~~~~~~~~~
 - Add "jupyter" renderer which uses JupyterChart for rendering (#3283). See :ref:`renderers` for more information.
+- Add offline support for JupyterChart and the new "jupyter" renderer. See :ref:`user-guide-jupyterchart-offline`
+  for more information.
 - Docs: Add :ref:`section on dashboards <display_dashboards>` which have support for Altair (#3299)
 - Support restrictive FIPS-compliant environment (#3291)
 
diff --git a/doc/user_guide/display_frontends.rst b/doc/user_guide/display_frontends.rst
index bb488986a..06c689011 100644
--- a/doc/user_guide/display_frontends.rst
+++ b/doc/user_guide/display_frontends.rst
@@ -50,6 +50,11 @@ The most used built-in renderers are:
   object explicitly following the instructions in the :ref:`user-guide-jupyterchart`
   documentation.
 
+``alt.renderers.enable("jupyter", offline=True)``
+  *(added in version 5.3):* Same as the ``"jupyter"`` renderer above, but loads JavaScript
+  dependencies from the ``vl-convert-python`` package (rather than from an online CDN)
+  so that an internet connection is not required.
+
 In addition, Altair includes the following renderers:
 
 - ``"default"``, ``"colab"``, ``"kaggle"``, ``"zeppelin"``: identical to ``"html"``
diff --git a/doc/user_guide/jupyter_chart.rst b/doc/user_guide/jupyter_chart.rst
index d2bcaa63a..d00327f3d 100644
--- a/doc/user_guide/jupyter_chart.rst
+++ b/doc/user_guide/jupyter_chart.rst
@@ -426,15 +426,33 @@ is used to combine the chart and HTML table in a column layout.
       Your browser does not support the video tag.
     </video>
 
+.. _user-guide-jupyterchart-offline:
+
+Offline Usage
+-------------
+By default, the ``JupyterChart`` widget loads its JavaScript dependencies dynamically from a CDN
+location, which requires an active internet connection. Starting in Altair 5.3, JupyterChart supports
+loading its JavaScript dependencies from the ``vl-convert-python`` package, which enables offline usage.
+
+Offline mode is enabled using the ``JupyterChart.enable_offline`` class method.
+
+.. code-block:: python
+
+    import altair as alt
+    alt.JupyterChart.enable_offline()
+
+This only needs to be called once, after which all displayed JupyterCharts will operate in offline mode.
+
+Offline mode can be disabled by passing ``offline=False`` to this same method.
+
+.. code-block:: python
+
+    import altair as alt
+    alt.JupyterChart.enable_offline(offline=False)
+
 Limitations
 -----------
 
 Setting Selections
 ~~~~~~~~~~~~~~~~~~
 It's not currently possible to set selection states from Python.
-
-Internet Connection Required
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-The JupyterChart class currently loads its JavaScript dependencies dynamically from a CDN location.
-This keeps the ``altair`` package small, but it means that an internet connection is required
-to display JupyterChart instances. In the future, we would like to provide optional offline support.