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remove PCR duplicate #47

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yx-xu opened this issue Jul 21, 2022 · 2 comments
Open

remove PCR duplicate #47

yx-xu opened this issue Jul 21, 2022 · 2 comments

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@yx-xu
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yx-xu commented Jul 21, 2022

Hi!
I'm sorry to bother you. I followed Ribocoed workflow, but I don't know whether need to remove PCR duplicate after STAR alignment. Could you give me some suggestions? Thank you very much!
@zhengtaoxiao
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If you only want to identify the novel translating ORFs, PCR duplication removal is not necessary.

PCR duplications might influence the quantification of the translational level of ORFs。

@yx-xu
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yx-xu commented Oct 6, 2022

Get it! Thanks for your reply!

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@zhengtaoxiao @yx-xu