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Update 02-dataset_integration.Rmd
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Co-authored-by: Joshua Shapiro <[email protected]>
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sjspielman and jashapiro authored Dec 6, 2024
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Expand Up @@ -740,7 +740,7 @@ However, unlike `fastMNN`, `harmony` does not "back-calculate" corrected express
For input, `harmony` needs a couple pieces of information:

- First, `harmony` takes a batch-weighted PCA matrix to perform integration.
We already calculated a batch-weighted PCA matrix (our `merged_PCA` reduced dimension), we'll provide this variable.
We already calculated a batch-weighted PCA matrix (our `merged_PCA` reduced dimension), we'll provide this as the the input.
- Second, we need to tell `harmony` about the covariates to use - `sample` and `patient`.
To do this, we provide two arguments:
- `meta_data`, a data frame that contains covariates across samples.
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