error occur step 2

[linshutian02]

Hi,
Recently, I applicated stLFR sequencing in nasal samples, and I tried to assemble these metagenomes reads with MetaTrass, something errors occured in Step2, the log are following:

{'subparser_name': 'TB', 'cleanfq1': './dir1_cleandata/split_reads.1.fq.gz.clean.gz', 'cleanfq2': './dir1_cleandata/split_reads.2.fq.gz.clean.gz', 'thread': '20', 'parallel': '20', 'sample': 'test', 'ref_db': ‘’/database/Kraken2/kraken_database', 'genome_size': '/database/Kraken2/genome_list.txt', 'max_depth': '300', 'min_depth': '10', 'pe_length': '100', 'tp_density': '0.1', 'outdir': './', 'runnow': 'yes'} The folder .//all_command_shell/ has been ceated! [2022-01-23 09:52:08] ###step2.1 kraken classfying starting Can't locate /MetaTrass/tools/kraken2lib.pm at /MetaTrass/tools/kraken2 line 24. [2022-01-23 09:52:09] ###step2.1 kraken classfying end The folder .//all_command_shell/ has been ceated! The folder .//dir2_taxonomy has been ceated! [2022-01-23 09:52:09] ###step2.2 TABrefining starting failed to open .//dir2_taxonomy/kraken/test.C for read! exit ... [2022-01-23 09:52:09] ###step2.2 TABrefining end The folder .//all_command_shell/ has been ceated! !!!.//dir2_taxonomy/SSRlist/tax_reads_depth.txt not found! Please run the step2.2 TABrefiner at first!!! [2022-01-23 09:52:09] ###step2.3 ReadID2Fastq starting [2022-01-23 09:52:10] ###step2.3 ReadID2Fastq end

it seem that not found the file 'kraken2lib.pm'. What's the matter with it ?

Yours

[QYanwei]

It's seem that your Kraken2 doesn't work well, please make sure the Kraken2 is installed correctly.