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Merge pull request #5056 from Opentrons/develop
chore(Release): 2024-03-07
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| { | ||
| "content": "# flake8: noqa\n\nimport math\n\nmetadata = {\n 'protocolName': 'PCR Prep',\n 'author': 'Rami Farawi <[email protected]>',\n 'source': 'Custom Protocol Request',\n 'apiLevel': '2.13'\n}\n\n\ndef run(ctx):\n\n [num_samp,\n p300_mount, m20_mount] = get_values( # noqa: F821\n \"num_samp\", \"p300_mount\", \"m20_mount\")\n\n # num_samp = 16\n # p300_mount = 'left'\n # m20_mount = 'right'\n\n # labware\n tuberack = ctx.load_labware('opentrons_24_tuberack_2000ul', 4)\n\n source_plate = ctx.load_labware('pcrplate_96_wellplate_200ul', 1)\n middle_plate = ctx.load_labware('pcrplate_96_wellplate_200ul', 2)\n final_plate = ctx.load_labware('pcrplate_96_wellplate_200ul', 3)\n\n tip300 = [ctx.load_labware('opentrons_96_tiprack_300ul', slot)\n for slot in [6]]\n tip20 = [ctx.load_labware('opentrons_96_filtertiprack_20ul', slot)\n for slot in [10, 11]]\n\n # pipettes\n p300 = ctx.load_instrument('p300_single_gen2', p300_mount,\n tip_racks=tip300)\n m20 = ctx.load_instrument('p20_multi_gen2', m20_mount,\n tip_racks=tip20)\n\n # mapping\n mmx = tuberack['A1']\n primer = tuberack['A2']\n final_mmx = tuberack['A3']\n num_col = math.ceil(num_samp/8)\n\n # protocol\n ctx.comment('\\n---------------MAKING MASTERMIX----------------\\n\\n')\n mmx_vol = 9.1\n primer_vol = 5.57\n\n p300.pick_up_tip()\n p300.transfer(mmx_vol*num_samp*1.15, mmx, final_mmx, new_tip='never')\n p300.drop_tip()\n\n total_vol = num_samp*(primer_vol+mmx_vol)\n p300.pick_up_tip()\n p300.transfer(primer_vol*num_samp*1.15, primer, final_mmx, new_tip='never')\n p300.mix(20, total_vol if total_vol < 300 else 300, final_mmx)\n p300.drop_tip()\n\n vol_per_col_well = (mmx_vol+primer_vol)*12*1.1\n p300.pick_up_tip()\n for well in middle_plate.columns()[0]:\n p300.aspirate(vol_per_col_well, final_mmx)\n p300.dispense(vol_per_col_well, well)\n p300.drop_tip()\n\n ctx.comment('\\n---------------DISTRIBUTING MASTERMIX----------------\\n\\n')\n m20.pick_up_tip()\n for col in final_plate.rows()[0][:num_col]:\n m20.aspirate(14.7, middle_plate.rows()[0][0])\n m20.dispense(14.7, col)\n m20.drop_tip()\n\n ctx.comment('\\n---------------DISTRIBUTING SAMPLE----------------\\n\\n')\n for s, d in zip(source_plate.rows()[0][:num_col], final_plate.rows()[0]):\n m20.pick_up_tip()\n m20.aspirate(4, s)\n m20.dispense(4, d)\n m20.mix(5, 15, d)\n m20.drop_tip()\n", | ||
| "content": "# flake8: noqa\n\nimport math\n\nmetadata = {\n 'protocolName': 'PCR Prep',\n 'author': 'Rami Farawi <[email protected]>',\n 'source': 'Custom Protocol Request',\n 'apiLevel': '2.13'\n}\n\n\ndef run(ctx):\n\n [num_samp,\n p300_mount, m20_mount] = get_values( # noqa: F821\n \"num_samp\", \"p300_mount\", \"m20_mount\")\n\n # num_samp = 16\n # p300_mount = 'left'\n # m20_mount = 'right'\n\n # labware\n tuberack = ctx.load_labware('opentrons_24_tuberack_2000ul', 4)\n\n source_plate = ctx.load_labware('pcrplate_96_wellplate_200ul', 1)\n middle_plate = ctx.load_labware('pcrplate_96_wellplate_200ul', 2)\n final_plate = ctx.load_labware('pcrplate_96_wellplate_200ul', 3)\n\n tip300 = [ctx.load_labware('opentrons_96_tiprack_300ul', slot)\n for slot in [6]]\n tip20 = [ctx.load_labware('opentrons_96_filtertiprack_20ul', slot)\n for slot in [10, 11]]\n\n # pipettes\n p300 = ctx.load_instrument('p300_single_gen2', p300_mount,\n tip_racks=tip300)\n m20 = ctx.load_instrument('p20_multi_gen2', m20_mount,\n tip_racks=tip20)\n\n # mapping\n mmx = tuberack['A1']\n primer = tuberack['A2']\n final_mmx = tuberack['A3']\n num_col = math.ceil(num_samp/8)\n\n # protocol\n ctx.comment('\\n---------------MAKING MASTERMIX----------------\\n\\n')\n mmx_vol = 9.1\n primer_vol = 5.57\n\n p300.pick_up_tip()\n p300.transfer(mmx_vol*num_samp*1.15, mmx, final_mmx, new_tip='never')\n p300.drop_tip()\n\n total_vol = num_samp*(primer_vol+mmx_vol)\n p300.pick_up_tip()\n p300.transfer(primer_vol*num_samp*1.15, primer, final_mmx, new_tip='never')\n for _ in range(20):\n p300.aspirate(total_vol if total_vol < 300 else 300, final_mmx)\n p300.dispense(total_vol if total_vol < 300 else 300, final_mmx.top(z=-3))\n p300.mix(20, total_vol if total_vol < 300 else 300, final_mmx)\n p300.drop_tip()\n\n vol_per_col_well = (mmx_vol+primer_vol)*12*1.1\n p300.pick_up_tip()\n for well in middle_plate.columns()[0]:\n p300.aspirate(vol_per_col_well, final_mmx)\n p300.dispense(vol_per_col_well, well)\n p300.drop_tip()\n\n ctx.comment('\\n---------------DISTRIBUTING MASTERMIX----------------\\n\\n')\n m20.pick_up_tip()\n for col in final_plate.rows()[0][:num_col]:\n m20.aspirate(14.7, middle_plate.rows()[0][0])\n m20.dispense(14.7, col)\n m20.drop_tip()\n\n ctx.comment('\\n---------------DISTRIBUTING SAMPLE----------------\\n\\n')\n for s, d in zip(source_plate.rows()[0][:num_col], final_plate.rows()[0]):\n m20.pick_up_tip()\n m20.aspirate(4, s)\n m20.dispense(4, d)\n m20.mix(5, 15, d)\n m20.drop_tip()\n", | ||
| "custom_labware_defs": [ | ||
| { | ||
| "brand": { | ||
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