Refactor

README.md

@@ -1,37 +1,51 @@
-# valody
-A tool to assign VAginaL DYnamic categories to individuals based on their Valencia CST assignments
+# VALODY
+A tool to assign VAginaL DYnamic categories to individuals based on their
+[VALENCIA](https://github.com/ravel-lab/VALENCIA) CST assignments.
 
 # Citation
 If using any part of this work, please cite "Hugerth LW, Krog MC et al, submitted"
 
 # The theory
-Vaginal microbiomes are tightly linked to the menstrual cycle, through menstrual bleeding and/or changes to the mucosal lining associated to estrogen levels. These microbiomes can also be affected by other factors, such as intercourse, hygiene, medication and stochastic processes. VALODY is designed to classify vaginal time-series according to the frequency and temporal pattern of their [community state types](https://github.com/ravel-lab/VALENCIA) in relation to subjects' menstrual cycles:
+Vaginal microbiomes are tightly linked to the menstrual cycle, through
+menstrual bleeding and/or changes to the mucosal lining associated with estrogen
+levels. These microbiomes can also be affected by other factors, such as
+intercourse, hygiene, medication and stochastic processes. VALODY is designed
+to classify vaginal time-series according to the frequency and temporal pattern
+of their [community state types](https://github.com/ravel-lab/VALENCIA) in
+relation to subjects' menstrual cycles:
 
 ![The Valody workflow](valody_outline.png)
 
 The user must therefore define:
 * which CST are considered eubiotic (by default: I, II and V)
 * Which days of the cycle are affected by menses. 
 
-Empirically, we find that the last 2-3 of the luteal phase should be included in the "perimenstrual" period, so that in a 28 day cycle, days 1-8 as well as 26-28 are considered perimentrual and days 9-25 as "central". 
+Empirically, we find that the last 2-3 days of the luteal phase should be
+included in the "perimenstrual" period, so that in a 28 day cycle, days 1-8 as
+well as 26-28 are considered perimenstrual and days 9-25 as "central". 
 
 # In practice
-The inputs to valody are the output of [Valencia](https://github.com/ravel-lab/VALENCIA) as well as a comma-separated file with the format
+The inputs to valody are the output of
+[VALENCIA](https://github.com/ravel-lab/VALENCIA) as well as a comma-separated
+file with the format
+
 | sampleID | subjectID | menses |
 |----------|-----------|--------|
 | sample1  | subj1     | 1      |
 | sample2  | subj1     | 1      |
 |   ...    |  ...      | ...    |
 | sampleN  | subjM     | 0      |
 
-Where sample ID:s should be identical to the ones in the Valencia file and menses should be marked as 1 for perimentrual samples and 0 otherwise.
+Where sample IDs should be identical to the ones in the VALENCIA output file
+and menses should be marked as `1` for perimenstrual samples and `0` otherwise.
+
+VALODY can then be run as:
 
-Valody can then be run as:
+    ./valody.py -i INPUT -m METADATA -o OUTPUT [-d DYSBIOSIS] [-e EUBIOSIS] [-s] 
 
-    python path/to/valody/assign_dynamics.py -i INPUT -m METADATA -o OUTPUT [-d DYSBIOSIS] [-e EUBIOSIS] [-s] 
-    -i INPUT, --input INPUT, Path to VALENCIA output
+    -i INPUT, --input INPUT, path to VALENCIA output
     -m METADATA, --metadata METADATA, CSV file with 'sampleID,subjectID,menses', where menses takes 1 for yes and 0 for no
-    -o OUTPUT, --output OUTPUT, Output csv file prefix
+    -o OUTPUT, --output OUTPUT, Output csv file 
     -d DYSBIOSIS, --dysbiosis DYSBIOSIS, comma-separated list of CST or sub-CST considered dysbiotic. Default: I, II, V
     -e EUBIOSIS, --eubiosis EUBIOSIS, comma-separated list of CST or sub-CST considered eubiotic. Default: III, IV
     -s, --subtypes, optional: use CST subtypes instead of main types; requires eubiosis and dysbiosis argument