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Merge pull request #31 from eastgenomics/fix_report_text_spacing
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Update vcf2xls_nirvana.pl
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Yu-jinKim authored Jun 22, 2020
2 parents b91bed9 + 9b59e7f commit 46cbe8f
Showing 1 changed file with 4 additions and 4 deletions.
8 changes: 4 additions & 4 deletions resources/home/dnanexus/vcf2xls_nirvana.pl
Original file line number Diff line number Diff line change
Expand Up @@ -277,17 +277,17 @@ sub fill_QC_sheets {
$meta_stats{ 'AVG_coverage'} = $avg_plus_20x;
}

my $report_blurb = "Next Generation Sequencing (NGS) of the coding region (+/-5 bp) of the following genes (reference sequences) using the Illumina TruSight One sequencing panel (NB. Whole exon deletions/duplications and other large rearrangements are not detected with this method) : \n\n";
my $report_blurb = "Next Generation Sequencing (NGS) of the coding region (+/-5 bp) of the following genes (reference sequences) using the Illumina TruSight One sequencing panel (NB. Whole exon deletions/duplications and other large rearrangements are not detected with this method):\n\n";

$report_blurb .= join("; ", @gene_transcripts ) . "\n\n";
$report_blurb .= "$panel_coverage % of this panel was sequenced to a depth of 20X or greater (this includes homologous regions where reads do not map uniquely), with analytical sensitivity of 99.5-99.9% (95% confidence interval from benchmarking against GIAB HG001 reference material). ";
$report_blurb .= "$panel_coverage % of this panel was sequenced to a depth of 20X or greater (this includes homologous regions where reads do not map uniquely), with analytical sensitivity of 99.5-99.9% (95% confidence interval from benchmarking against GIAB HG001 reference material). ";

# Dementia needs different (additional) text:
if (index($gene_list{ 'PANEL_IDS'}, "Dementia") != -1) {
$report_blurb .= "Targeted analysis of the exon/intron boundary of exon 9 of the MAPT gene (NM_005910.5), a known hot-spot for pathogenic variants, has been performed.";
$report_blurb .= "Targeted analysis of the exon/intron boundary of exon 9 of the MAPT gene (NM_005910.5), a known hot-spot for pathogenic variants, has been performed. ";
}

$report_blurb .= " \n\nThe presence of variants reported above, except for variants of unknown significance, has been confirmed by Sanger sequencing. Variants with a population frequency greater than 1 in 500 for dominant conditions, and 1 in 50 for recessive disorders have been deemed insignificant and are not reported. Variants are named using HGVS nomenclature, where nucleotide 1 is the A of the ATG-translation initiation codon. Identification of variants present in NGS data was performed using the Dias pipeline.";
$report_blurb .= "The presence of variants reported above, except for variants of unknown significance, has been confirmed by Sanger sequencing. Variants with a population frequency greater than 1 in 500 for dominant conditions, and 1 in 50 for recessive disorders have been deemed insignificant and are not reported. Variants are named using HGVS nomenclature, where nucleotide 1 is the A of the ATG-translation initiation codon. Identification of variants present in NGS data was performed using the Dias pipeline.";

if ( $gene_list{ 'PANEL_IDS'} ) {
worksheet_write('Summary', 1 , 6 , "Panel(s) w/ id's", $$formatting{ 'bold' });
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