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Update README.md #20
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sreichl authored Nov 29, 2023
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Expand Up @@ -109,11 +109,20 @@ Additionally, the report zip archive of the hg38 test example is provided to sho
# Resources
- Data Resources: To ensure the reproducibility of results and to make the workflow accessible we provide all required reference data for the analysis of ATAC-seq samples for [human GRCh38 (hg38)](https://doi.org/10.5281/zenodo.6344173) and [mouse GRCm38 (mm10)](https://doi.org/10.5281/zenodo.6344321) genomes on Zendodo.
```console
# download Zenodo records using zenodo_get v1.3.4
# download Zenodo records using zenodo_get

# install zenodo_get v1.3.4
conda install -c conda-forge zenodo_get=1.3.4

# human GRCh38 (hg38)
zenodo_get --record 6344173 --output-dir=resources/atacseq_pipeline/hg38/
cd resources/atacseq_pipeline/hg38
unzip indices_for_Bowtie2.zip && rm indices_for_Bowtie2.zip

# mouse GRCm38 (mm10)
zenodo_get --record 6344322 --output-dir=resources/atacseq_pipeline/mm10/
cd resources/atacseq_pipeline/mm10
unzip indices_for_Bowtie2.zip && rm indices_for_Bowtie2.zip
```
- Recommended [MR.PARETO](https://github.com/epigen/mr.pareto) modules for downstream analyses (in that order):
- [<ins>Sp</ins>lit, F<ins>ilter</ins>, Norma<ins>lize</ins> and <ins>Integrate</ins> Sequencing Data](https://github.com/epigen/spilterlize_integrate/) after quantification.
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