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sreichl authored Nov 6, 2024
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[![MR.PARETO](https://img.shields.io/badge/MR.PARETO-red)](https://github.com/epigen/mr.pareto/)
[![MrBiomics](https://img.shields.io/badge/MrBiomics-red)](https://github.com/epigen/MrBiomics/)
[![DOI](https://zenodo.org/badge/483638364.svg)](https://zenodo.org/doi/10.5281/zenodo.10689139)
[![](https://tokei.rs/b1/github/epigen/dea_seurat?category=code)]()
[![](https://tokei.rs/b1/github/epigen/dea_seurat?category=files)]()
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A [Snakemake 8](https://snakemake.readthedocs.io/en/stable/) workflow for performing differential expression analyses (DEA) of processed (multimodal) scRNA-seq data powered by the R package [Seurat's](https://satijalab.org/seurat/index.html) functions [FindMarkers](https://satijalab.org/seurat/reference/findmarkers) and [FindAllMarkers](https://satijalab.org/seurat/reference/findallmarkers).

> [!NOTE]
> This workflow adheres to the module specifications of [MR.PARETO](https://github.com/epigen/mr.pareto), an effort to augment research by modularizing (biomedical) data science. For more details, instructions, and modules check out the project's repository.
> This workflow adheres to the module specifications of [MrBiomics](https://github.com/epigen/MrBiomics), an effort to augment research by modularizing (biomedical) data science. For more details, instructions, and modules check out the project's repository.
>
> ⭐️ **Star and share modules you find valuable** 📤 - help others discover them, and guide our future work!
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- samples/patients: 15
- cells: 21657
- features (genes): 22276
- preprocessed using the compatible MR.PARETO module for [scRNA-seq data processing & visualization](https://github.com/epigen/scrnaseq_processing_seurat)
- preprocessed using the compatible MrBiomics module for [scRNA-seq data processing & visualization](https://github.com/epigen/scrnaseq_processing_seurat)
- We performed a 1 vs rest analysis using the cell type annotation ("ALL").
- total runtime on HPC w/ SLURM (32GB RAM; only DEA with 8 cores otherwise 1 core): <25 minutes for 17 jobs in total

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- [Snakemake Workflow Catalog Entry](https://snakemake.github.io/snakemake-workflow-catalog?usage=epigen/dea_seurat)

# 📚 Resources
- Recommended compatible [MR.PARETO](https://github.com/epigen/mr.pareto) modules for:
- Recommended compatible [MrBiomics](https://github.com/epigen/MrBiomics) modules for:
- upstream analysis
- [scRNA-seq Data Processing & Visualization](https://github.com/epigen/scrnaseq_processing_seurat) for processing (multimodal) single-cell transcriptome data.
- downstream analyses
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