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Merge pull request drieslab#886 from sjcshin/suite
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Update figures in visium cytassist lung cancer
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josschavezf authored Feb 1, 2024
2 parents f8fc71f + 61e1f71 commit 5256826
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6 changes: 1 addition & 5 deletions vignettes/visium_cytassist_lungcancer.Rmd
Original file line number Diff line number Diff line change
Expand Up @@ -298,7 +298,6 @@ giotto_SC <- Giotto::loadGiotto("sc_lung_carcinoma")

The cell-type specific signature gene list was obtained from a previous study focused on investigating the therapy-induced evolution of lung cancer revealed by single-cell RNA sequencing. More information about the paper and scRNA-seq dataset can be found [here](https://doi.org/10.1016/j.cell.2020.07.017).


```{r, eval=FALSE}
# umap plots
# Create PAGE matrix
Expand Down Expand Up @@ -331,9 +330,6 @@ signature_matrix = makeSignMatrixPAGE(sign_names = c('T_Cells', 'Macrophage', 'D
pdcs_markers))
# 1.3 enrichment test with PAGE
giotto_SC = getSpatialDataset(dataset = 'mol_cart_lung_873_C1', directory = data_path, method = "curl")
markers_scran = findMarkers_one_vs_all(gobject=giotto_SC, method="scran",
expression_values="normalized", cluster_column = "leiden_clus", min_feats=3)
Expand Down Expand Up @@ -480,7 +476,7 @@ heatmSpatialCorFeats(visium_lungcancer,
heatmap_legend_param = list(title = NULL))
```

![](images/visium_cytassist_lungcancer/22-z1-heatmap_correlated_genes.pdf){width="50.0%"}
![](images/visium_cytassist_lungcancer/22-z1-heatmap_correlated_genes.png){width="50.0%"}

```{r, eval=FALSE}
# 4. rank spatial correlated clusters and show genes for selected clusters
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