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Updated differential expression heatmaps, logOR filtering, and bugfixes

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@drewjbeh drewjbeh released this 30 Apr 13:43
· 261 commits to master since this release

New features and bugfixes

Differential expression heatmaps

  • New heatmaps in DESeq2 folders which show scaled expression (normalised DESeq2 read counts), and log2 fold change values for all conditions vs control condition for significantly differentially expressed genes (padj <= 0.05).
  • Line plot of basemean expression include on far right.
  • See DE_isodecodersScaled_hm.pdf and DE_anticodonScaled_hm.pdf in /DESeq2/isodecoder and /DESeq2/anticodon, respectively.

Normalised counts outputs

  • Tables of DESeq2 normalised counts for isodecoders and anticodons now output to /counts folder.
  • These are the same values present in the last columns of all DESeq2 diffexpr-results.csv files, which means they are also excluding undeconvoluted clusters.

Bugfixes

  • Fixed ZeroDivisionError in mmQuant.py for 0 coverage at cluster positions - see issue #7
  • Fixed errors for very sparse data plotting issues in modPlot.py - see #6