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Jan 27, 2022
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| <!DOCTYPE html> | ||
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@@ -22,7 +20,7 @@ <h3>Introduction</h3> | |
| of disease-linked mutation. | ||
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| <h3>Commptational method</h3> | ||
| <h3>Computational method</h3> | ||
| <p> | ||
| We used the command-line version of the <a href="https://services.healthtech.dtu.dk/service.php?SignalP-4.1">SignalP4.1 program</a>. The | ||
| program takes a sequence (FASTA formatted) of 70 amino acids length as input and predicts the | ||
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| <a href="https://pubmed.ncbi.nlm.nih.gov/14681372/">UniProt</a>, <a href="https://pubmed.ncbi.nlm.nih.gov/30371878/">COSMIC</a>, and <a href="https://pubmed.ncbi.nlm.nih.gov/29165669/">ClinVAR</a>. | ||
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| <h3>Validation experiments</h3> | ||
| <h3>Validation</h3> | ||
| <p> | ||
| Validation of the hits were then performed by ectopically expressing wt and mutant constructs in | ||
| wt HEK293T cells or lacking the regulatory SPC subunit SPCS1 and cell lysates were then analysed | ||
| via Western blot to detect possible SPCS1-dependent cleavage fragments (see methods for details). | ||
| </p> | ||
| <h3>Contacts</h3> | ||
| <h3>Contact</h3> | ||
| <p> | ||
| Gurdeep Singh: [email protected] (<a href="russelllab.org">Russell lab</a>, Heidelberg)<br> | ||
| Andrea Zanotti: [email protected] (<a href="https://biochemie-med.uni-koeln.de/en/research/research-groups/lemberg-lab">Lemberg lab</a>, Heidelberg/Cologne)<br> | ||
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