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PCR primer filter #4499

Merged
merged 51 commits into from
Jan 23, 2025
Merged

PCR primer filter #4499

merged 51 commits into from
Jan 23, 2025

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xchang1
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@xchang1 xchang1 commented Jan 20, 2025

Changelog Entry

To be copied to the draft changelog by merger:

  • Add vg primers to get stats about variants in PCR primers from primer3

Description

I'm finally getting around to merging @AlanZhangUCSC's rotation project from last year .
He added a subcommand vg primers that takes a PRC primer file from primer3 and outputs a tsv of pangenome stats, including the minimum and maximum distance between primer pairs and a measurement of how much variation is in the primers, based on the haplotypes.
I also added the option to map the template sequence with giraffe if the reference coordinates are not given or badly formatted.

@xchang1 xchang1 changed the title PRC primer filter PCR primer filter Jan 20, 2025
@adamnovak adamnovak merged commit 8f1c1d8 into master Jan 23, 2025
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4 participants