NovaCTF plugin

This plugin provides wrappers for several programs of NovaCTF software suite.

Installation

You will need to use 3.0+ version of Scipion to be able to run these protocols. To install the plugin, you have two options:

1. Stable version

   scipion installp -p scipion-em-novactf
   

2. Developer's version

   • download repository

   git clone -b devel https://github.com/scipion-em/scipion-em-novactf.git
   

   • install

   scipion installp -p /path/to/scipion-em-novactf --devel
   

NovaCTF binaries will be downloaded and installed automatically with the plugin, but you can also link an existing installation. Default installation path assumed is software/em/novactf-master, if you want to change it, set NOVACTF_HOME in scipion.conf file to the folder where the NovaCTF is installed.

To check the installation, simply run the test below:

scipion3 tests novactf.tests.test_protocols_novactf.TestNovaCtfReconstructionWorkflow

Supported versions

master (8 Nov 2018)

Protocols

• compute defocus array
• 3D CTF correction and reconstruction

References

1. Beata Turoňová and Florian K.M. Schur and William Wan and John A.G. Briggs. Efficient 3D-CTF correction for cryo-electron tomography using NovaCTF improves subtomogram averaging resolution to 3.4Å. Journal of Structural Biology 199 (3), p. 187-195, 2017. doi: https://doi.org/10.1016/j.jsb.2017.07.007